Experiment: RCH2_defined_glucose with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5 x; and Iron (II) 0.209 mg/mL 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
     
    
        
         
     
    
        
         
     
    
        
         
     
    
         
     
     
 
500 nt BWI76_RS25220 and BWI76_RS25225 are separated by 4 nucleotides BWI76_RS25225 and BWI76_RS25230 are separated by 7 nucleotides BWI76_RS25230 and BWI76_RS25235 are separated by 209 nucleotides  
        
        BWI76_RS25220: BWI76_RS25220 - outer membrane lipid asymmetry maintenance protein MlaD, at 5,245,054 to 5,245,605 
         
        _RS25220 
         
        
        BWI76_RS25225: BWI76_RS25225 - ABC transporter permease, at 5,245,610 to 5,246,392 
         
        _RS25225 
         
        
        BWI76_RS25230: BWI76_RS25230 - phospholipid ABC transporter ATP-binding protein MlaF, at 5,246,400 to 5,247,212 
         
        _RS25230 
         
        
        BWI76_RS25235: BWI76_RS25235 - calcium/sodium antiporter, at 5,247,422 to 5,248,399 
         
        _RS25235 
         Position (kb)  
5246 
 
5247 
 
5248 Strain fitness (log2 ratio)  
-5 
 
-4 
 
-3 
 
-2 
 
-1 
 
0 
 
1 at 5245.572 kb on + strand at 5246.024 kb on + strand, within BWI76_RS25225 at 5246.161 kb on + strand, within BWI76_RS25225 at 5246.241 kb on + strand, within BWI76_RS25225 at 5246.241 kb on + strand, within BWI76_RS25225 at 5246.252 kb on - strand, within BWI76_RS25225 at 5246.294 kb on + strand, within BWI76_RS25225 at 5246.338 kb on - strand at 5246.538 kb on - strand, within BWI76_RS25230 at 5246.629 kb on + strand, within BWI76_RS25230 at 5246.740 kb on + strand, within BWI76_RS25230 at 5246.845 kb on - strand, within BWI76_RS25230 at 5246.909 kb on + strand, within BWI76_RS25230 at 5246.947 kb on - strand, within BWI76_RS25230 at 5246.968 kb on + strand, within BWI76_RS25230 at 5246.976 kb on - strand, within BWI76_RS25230 at 5246.976 kb on - strand, within BWI76_RS25230 at 5246.976 kb on - strand, within BWI76_RS25230 at 5247.247 kb on - strand at 5247.379 kb on - strand at 5247.432 kb on - strand at 5247.487 kb on + strand at 5247.487 kb on + strand at 5247.487 kb on + strand at 5247.487 kb on + strand at 5247.553 kb on + strand, within BWI76_RS25235 at 5247.605 kb on - strand, within BWI76_RS25235 at 5247.633 kb on + strand, within BWI76_RS25235 at 5247.699 kb on - strand, within BWI76_RS25235 at 5247.883 kb on - strand, within BWI76_RS25235 at 5247.883 kb on - strand at 5247.998 kb on + strand, within BWI76_RS25235 at 5248.166 kb on + strand, within BWI76_RS25235  
 
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction   RCH2_defined_glucose with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5 x; and Iron (II) 0.209 mg/mL             remove   5,245,572   +        -2.8   5,246,024   +    BWI76_RS25225   0.53  -4.2   5,246,161   +    BWI76_RS25225   0.70  -1.3   5,246,241   +    BWI76_RS25225   0.81  -4.6   5,246,241   +    BWI76_RS25225   0.81  -2.7   5,246,252   -    BWI76_RS25225   0.82  -5.7   5,246,294   +    BWI76_RS25225   0.87  -2.5   5,246,338   -        -2.6   5,246,538   -    BWI76_RS25230   0.17  -3.3   5,246,629   +    BWI76_RS25230   0.28  -3.6   5,246,740   +    BWI76_RS25230   0.42  -3.4   5,246,845   -    BWI76_RS25230   0.55  -2.6   5,246,909   +    BWI76_RS25230   0.63  -2.8   5,246,947   -    BWI76_RS25230   0.67  -4.0   5,246,968   +    BWI76_RS25230   0.70  -3.8   5,246,976   -    BWI76_RS25230   0.71  -2.7   5,246,976   -    BWI76_RS25230   0.71  -3.8   5,246,976   -    BWI76_RS25230   0.71  -5.3   5,247,247   -        +0.3   5,247,379   -        -0.2   5,247,432   -        -0.4   5,247,487   +        +0.4   5,247,487   +        +1.1   5,247,487   +        +0.7   5,247,487   +        -0.4   5,247,553   +    BWI76_RS25235   0.13  +0.1   5,247,605   -    BWI76_RS25235   0.19  -0.2   5,247,633   +    BWI76_RS25235   0.22  -0.1   5,247,699   -    BWI76_RS25235   0.28  +0.1   5,247,883   -    BWI76_RS25235   0.47  +0.2   5,247,883   -        +0.3   5,247,998   +    BWI76_RS25235   0.59  +0.1   5,248,166   +    BWI76_RS25235   0.76  -0.6 
 
Or see this region's nucleotide sequence