Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS23940

Experiment: RCH2_defined_glucose with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5 x; and Iron (II) 0.209 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS23935 and BWI76_RS23940 overlap by 8 nucleotidesBWI76_RS23940 and BWI76_RS23945 are separated by 10 nucleotidesBWI76_RS23945 and BWI76_RS23950 are separated by 24 nucleotides BWI76_RS23935: BWI76_RS23935 - methylmalonyl-CoA mutase, at 4,994,947 to 4,997,091 _RS23935 BWI76_RS23940: BWI76_RS23940 - ATPase/protein kinase, at 4,997,084 to 4,998,079 _RS23940 BWI76_RS23945: BWI76_RS23945 - methylmalonyl-CoA decarboxylase, at 4,998,090 to 4,998,875 _RS23945 BWI76_RS23950: BWI76_RS23950 - succinate CoA transferase, at 4,998,900 to 5,000,366 _RS23950 Position (kb) 4997 4998 4999Strain fitness (log2 ratio) -1 0 1at 4996.117 kb on - strand, within BWI76_RS23935at 4996.176 kb on + strand, within BWI76_RS23935at 4996.298 kb on - strand, within BWI76_RS23935at 4996.367 kb on + strand, within BWI76_RS23935at 4996.375 kb on - strand, within BWI76_RS23935at 4996.454 kb on + strand, within BWI76_RS23935at 4996.528 kb on - strand, within BWI76_RS23935at 4996.587 kb on + strand, within BWI76_RS23935at 4996.781 kb on + strand, within BWI76_RS23935at 4996.795 kb on - strand, within BWI76_RS23935at 4996.909 kb on - strandat 4997.038 kb on + strandat 4997.148 kb on - strandat 4997.426 kb on + strand, within BWI76_RS23940at 4997.519 kb on + strand, within BWI76_RS23940at 4997.519 kb on + strand, within BWI76_RS23940at 4997.701 kb on - strand, within BWI76_RS23940at 4997.701 kb on - strand, within BWI76_RS23940at 4997.947 kb on + strand, within BWI76_RS23940at 4998.334 kb on - strand, within BWI76_RS23945at 4998.407 kb on + strand, within BWI76_RS23945at 4998.806 kb on - strandat 4998.891 kb on - strandat 4998.891 kb on - strandat 4998.891 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_glucose with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5 x; and Iron (II) 0.209 mg/mL
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4,996,117 - BWI76_RS23935 0.55 +0.6
4,996,176 + BWI76_RS23935 0.57 -1.0
4,996,298 - BWI76_RS23935 0.63 -0.0
4,996,367 + BWI76_RS23935 0.66 -0.4
4,996,375 - BWI76_RS23935 0.67 +0.3
4,996,454 + BWI76_RS23935 0.70 -0.1
4,996,528 - BWI76_RS23935 0.74 +0.4
4,996,587 + BWI76_RS23935 0.76 -0.5
4,996,781 + BWI76_RS23935 0.86 +0.9
4,996,795 - BWI76_RS23935 0.86 -0.2
4,996,909 - -0.7
4,997,038 + +0.1
4,997,148 - +0.2
4,997,426 + BWI76_RS23940 0.34 -0.6
4,997,519 + BWI76_RS23940 0.44 -0.3
4,997,519 + BWI76_RS23940 0.44 -0.4
4,997,701 - BWI76_RS23940 0.62 -0.0
4,997,701 - BWI76_RS23940 0.62 +0.8
4,997,947 + BWI76_RS23940 0.87 +0.2
4,998,334 - BWI76_RS23945 0.31 -0.3
4,998,407 + BWI76_RS23945 0.40 -0.4
4,998,806 - +0.3
4,998,891 - +0.5
4,998,891 - -0.3
4,998,891 - +0.5

Or see this region's nucleotide sequence