Experiment: RCH2_defined_glucose with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5 x; and Iron (II) 0.209 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00840 and BWI76_RS00845 are separated by 184 nucleotides BWI76_RS00845 and BWI76_RS00850 are separated by 9 nucleotides BWI76_RS00850 and BWI76_RS00855 are separated by 77 nucleotides
BWI76_RS00840: BWI76_RS00840 - acetylornithine deacetylase, at 173,585 to 174,736
_RS00840
BWI76_RS00845: BWI76_RS00845 - N-acetyl-gamma-glutamyl-phosphate reductase, at 174,921 to 175,925
_RS00845
BWI76_RS00850: BWI76_RS00850 - acetylglutamate kinase, at 175,935 to 176,708
_RS00850
BWI76_RS00855: BWI76_RS00855 - argininosuccinate lyase, at 176,786 to 178,159
_RS00855
Position (kb) 174
175
176 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 173.960 kb on - strand, within BWI76_RS00840 at 173.960 kb on - strand, within BWI76_RS00840 at 174.257 kb on + strand, within BWI76_RS00840 at 174.352 kb on + strand, within BWI76_RS00840 at 174.432 kb on - strand, within BWI76_RS00840 at 174.432 kb on - strand, within BWI76_RS00840 at 174.445 kb on + strand, within BWI76_RS00840 at 174.445 kb on + strand, within BWI76_RS00840 at 174.453 kb on - strand, within BWI76_RS00840 at 174.497 kb on - strand, within BWI76_RS00840 at 174.674 kb on + strand at 174.707 kb on - strand at 174.966 kb on + strand at 174.996 kb on + strand at 175.207 kb on + strand, within BWI76_RS00845 at 175.291 kb on + strand, within BWI76_RS00845 at 175.291 kb on + strand, within BWI76_RS00845 at 175.329 kb on - strand, within BWI76_RS00845 at 175.483 kb on - strand, within BWI76_RS00845 at 175.498 kb on - strand, within BWI76_RS00845 at 175.593 kb on + strand, within BWI76_RS00845 at 175.709 kb on - strand, within BWI76_RS00845 at 175.714 kb on - strand, within BWI76_RS00845 at 175.739 kb on + strand, within BWI76_RS00845 at 175.775 kb on - strand, within BWI76_RS00845 at 175.803 kb on + strand, within BWI76_RS00845 at 175.825 kb on - strand at 176.129 kb on + strand, within BWI76_RS00850 at 176.171 kb on + strand, within BWI76_RS00850 at 176.235 kb on - strand, within BWI76_RS00850 at 176.263 kb on + strand, within BWI76_RS00850 at 176.350 kb on + strand, within BWI76_RS00850 at 176.350 kb on + strand, within BWI76_RS00850 at 176.358 kb on - strand, within BWI76_RS00850 at 176.583 kb on - strand, within BWI76_RS00850 at 176.612 kb on - strand, within BWI76_RS00850 at 176.652 kb on + strand at 176.655 kb on - strand at 176.669 kb on + strand at 176.669 kb on + strand at 176.737 kb on + strand at 176.861 kb on + strand at 176.876 kb on + strand at 176.917 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5 x; and Iron (II) 0.209 mg/mL remove 173,960 - BWI76_RS00840 0.33 -0.4 173,960 - BWI76_RS00840 0.33 -0.8 174,257 + BWI76_RS00840 0.58 -0.5 174,352 + BWI76_RS00840 0.67 -1.0 174,432 - BWI76_RS00840 0.74 -0.7 174,432 - BWI76_RS00840 0.74 -0.3 174,445 + BWI76_RS00840 0.75 -0.3 174,445 + BWI76_RS00840 0.75 -0.7 174,453 - BWI76_RS00840 0.75 -0.3 174,497 - BWI76_RS00840 0.79 -0.8 174,674 + -0.2 174,707 - +0.3 174,966 + -2.2 174,996 + -1.8 175,207 + BWI76_RS00845 0.28 -1.5 175,291 + BWI76_RS00845 0.37 -1.8 175,291 + BWI76_RS00845 0.37 -0.9 175,329 - BWI76_RS00845 0.41 -2.3 175,483 - BWI76_RS00845 0.56 -2.8 175,498 - BWI76_RS00845 0.57 -2.1 175,593 + BWI76_RS00845 0.67 -2.8 175,709 - BWI76_RS00845 0.78 -3.8 175,714 - BWI76_RS00845 0.79 -3.9 175,739 + BWI76_RS00845 0.81 -3.3 175,775 - BWI76_RS00845 0.85 -2.0 175,803 + BWI76_RS00845 0.88 -2.9 175,825 - -3.1 176,129 + BWI76_RS00850 0.25 -2.0 176,171 + BWI76_RS00850 0.30 -3.2 176,235 - BWI76_RS00850 0.39 -2.6 176,263 + BWI76_RS00850 0.42 -2.2 176,350 + BWI76_RS00850 0.54 -3.3 176,350 + BWI76_RS00850 0.54 -3.1 176,358 - BWI76_RS00850 0.55 -3.4 176,583 - BWI76_RS00850 0.84 -3.1 176,612 - BWI76_RS00850 0.87 -2.5 176,652 + -0.6 176,655 - -3.1 176,669 + -2.4 176,669 + -2.7 176,737 + -0.1 176,861 + -3.6 176,876 + -2.9 176,917 + -1.8
Or see this region's nucleotide sequence