Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS20620 and HSERO_RS20625 are separated by 17 nucleotides HSERO_RS20625 and HSERO_RS20630 are separated by 63 nucleotides HSERO_RS20630 and HSERO_RS20635 are separated by 22 nucleotides
HSERO_RS20620: HSERO_RS20620 - phenylacetic acid degradation protein, at 4,728,633 to 4,729,385
_RS20620
HSERO_RS20625: HSERO_RS20625 - phenylacetate-CoA oxygenase, at 4,729,403 to 4,729,927
_RS20625
HSERO_RS20630: HSERO_RS20630 - phenylacetic acid degradation protein, at 4,729,991 to 4,731,061
_RS20630
HSERO_RS20635: HSERO_RS20635 - aldehyde dehydrogenase, at 4,731,084 to 4,732,769
_RS20635
Position (kb) 4729
4730
4731
4732 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 4729.328 kb on - strand at 4729.379 kb on + strand at 4729.387 kb on - strand at 4729.609 kb on + strand, within HSERO_RS20625 at 4729.675 kb on + strand, within HSERO_RS20625 at 4730.024 kb on - strand at 4730.261 kb on + strand, within HSERO_RS20630 at 4730.425 kb on + strand, within HSERO_RS20630 at 4730.871 kb on + strand, within HSERO_RS20630 at 4731.165 kb on + strand at 4731.270 kb on + strand, within HSERO_RS20635 at 4731.273 kb on - strand, within HSERO_RS20635 at 4731.298 kb on + strand, within HSERO_RS20635 at 4731.306 kb on - strand, within HSERO_RS20635 at 4731.623 kb on + strand, within HSERO_RS20635 at 4731.758 kb on + strand, within HSERO_RS20635 at 4731.780 kb on - strand, within HSERO_RS20635 at 4731.806 kb on + strand, within HSERO_RS20635
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI remove 4,729,328 - +0.4 4,729,379 + -1.0 4,729,387 - -2.2 4,729,609 + HSERO_RS20625 0.39 +0.6 4,729,675 + HSERO_RS20625 0.52 -3.5 4,730,024 - -0.3 4,730,261 + HSERO_RS20630 0.25 -1.4 4,730,425 + HSERO_RS20630 0.41 -3.2 4,730,871 + HSERO_RS20630 0.82 -2.5 4,731,165 + +0.4 4,731,270 + HSERO_RS20635 0.11 -0.7 4,731,273 - HSERO_RS20635 0.11 +0.0 4,731,298 + HSERO_RS20635 0.13 -0.7 4,731,306 - HSERO_RS20635 0.13 +0.2 4,731,623 + HSERO_RS20635 0.32 +1.2 4,731,758 + HSERO_RS20635 0.40 +0.3 4,731,780 - HSERO_RS20635 0.41 +0.4 4,731,806 + HSERO_RS20635 0.43 +0.7
Or see this region's nucleotide sequence