Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS10295 and HSERO_RS10300 are separated by 38 nucleotides HSERO_RS10300 and HSERO_RS10305 are separated by 53 nucleotides HSERO_RS10305 and HSERO_RS10310 overlap by 8 nucleotides
HSERO_RS10295: HSERO_RS10295 - ATP synthase, at 2,319,713 to 2,321,119
_RS10295
HSERO_RS10300: HSERO_RS10300 - flagellar assembly protein FliH, at 2,321,158 to 2,321,859
_RS10300
HSERO_RS10305: HSERO_RS10305 - flagellar motor switch protein FliG, at 2,321,913 to 2,322,908
_RS10305
HSERO_RS10310: HSERO_RS10310 - flagellar M-ring protein FliF, at 2,322,901 to 2,324,682
_RS10310
Position (kb)
2321
2322
2323 Strain fitness (log2 ratio)
-1
0
1 at 2320.921 kb on + strand, within HSERO_RS10295 at 2321.134 kb on - strand at 2321.313 kb on + strand, within HSERO_RS10300 at 2321.881 kb on - strand at 2321.901 kb on + strand at 2322.144 kb on + strand, within HSERO_RS10305 at 2322.264 kb on + strand, within HSERO_RS10305 at 2322.418 kb on - strand, within HSERO_RS10305 at 2322.418 kb on - strand, within HSERO_RS10305 at 2322.666 kb on + strand, within HSERO_RS10305 at 2322.953 kb on + strand at 2322.986 kb on + strand at 2322.986 kb on + strand at 2322.994 kb on - strand at 2322.994 kb on - strand at 2323.038 kb on - strand at 2323.208 kb on + strand, within HSERO_RS10310 at 2323.286 kb on + strand, within HSERO_RS10310 at 2323.502 kb on + strand, within HSERO_RS10310 at 2323.525 kb on + strand, within HSERO_RS10310 at 2323.546 kb on + strand, within HSERO_RS10310
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI remove 2,320,921 + HSERO_RS10295 0.86 -1.0 2,321,134 - +0.1 2,321,313 + HSERO_RS10300 0.22 -1.2 2,321,881 - +1.2 2,321,901 + -0.7 2,322,144 + HSERO_RS10305 0.23 -1.2 2,322,264 + HSERO_RS10305 0.35 -1.5 2,322,418 - HSERO_RS10305 0.51 -1.3 2,322,418 - HSERO_RS10305 0.51 -0.0 2,322,666 + HSERO_RS10305 0.76 -1.5 2,322,953 + -0.3 2,322,986 + -1.4 2,322,986 + -1.1 2,322,994 - -0.6 2,322,994 - -1.7 2,323,038 - +0.2 2,323,208 + HSERO_RS10310 0.17 -0.9 2,323,286 + HSERO_RS10310 0.22 -1.1 2,323,502 + HSERO_RS10310 0.34 -1.6 2,323,525 + HSERO_RS10310 0.35 -0.1 2,323,546 + HSERO_RS10310 0.36 -1.4
Or see this region's nucleotide sequence