Experiment: Recovery broth with Streptomycin 0.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS20480 and BBR_RS21170 overlap by 4 nucleotides BBR_RS21170 and BBR_RS20485 are separated by 3 nucleotides BBR_RS20485 and BBR_RS20490 are separated by 31 nucleotides
BBR_RS20480: BBR_RS20480 - membrane protein, at 2,420,522 to 2,421,532
_RS20480
BBR_RS21170: BBR_RS21170 - membrane protein insertion efficiency factor YidD, at 2,421,529 to 2,421,846
_RS21170
BBR_RS20485: BBR_RS20485 - ribonuclease P protein component, at 2,421,850 to 2,422,200
_RS20485
BBR_RS20490: BBR_RS20490 - 50S ribosomal protein L34, at 2,422,232 to 2,422,366
_RS20490
Position (kb)
2421
2422 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2420.600 kb on - strand at 2420.634 kb on - strand, within BBR_RS20480 at 2421.470 kb on - strand at 2421.482 kb on - strand at 2421.497 kb on - strand at 2421.521 kb on - strand at 2421.551 kb on - strand at 2421.551 kb on - strand at 2421.551 kb on - strand at 2421.551 kb on - strand at 2421.551 kb on - strand at 2421.551 kb on - strand at 2421.551 kb on - strand at 2421.551 kb on - strand at 2421.551 kb on - strand at 2421.551 kb on - strand at 2421.551 kb on - strand at 2421.551 kb on - strand at 2421.572 kb on - strand, within BBR_RS21170 at 2421.577 kb on - strand, within BBR_RS21170 at 2421.577 kb on - strand, within BBR_RS21170 at 2421.585 kb on - strand, within BBR_RS21170 at 2421.587 kb on - strand, within BBR_RS21170 at 2421.587 kb on - strand, within BBR_RS21170 at 2421.587 kb on - strand, within BBR_RS21170 at 2421.587 kb on - strand, within BBR_RS21170 at 2421.595 kb on - strand, within BBR_RS21170 at 2421.595 kb on - strand, within BBR_RS21170 at 2421.630 kb on - strand, within BBR_RS21170 at 2421.644 kb on - strand, within BBR_RS21170 at 2421.644 kb on - strand, within BBR_RS21170 at 2421.660 kb on - strand, within BBR_RS21170 at 2421.660 kb on - strand, within BBR_RS21170 at 2421.660 kb on - strand, within BBR_RS21170 at 2421.660 kb on - strand, within BBR_RS21170 at 2421.693 kb on - strand, within BBR_RS21170 at 2421.699 kb on - strand, within BBR_RS21170 at 2421.699 kb on - strand, within BBR_RS21170 at 2421.699 kb on - strand, within BBR_RS21170 at 2421.699 kb on - strand, within BBR_RS21170 at 2421.699 kb on - strand, within BBR_RS21170 at 2421.715 kb on - strand, within BBR_RS21170 at 2421.715 kb on - strand, within BBR_RS21170 at 2421.715 kb on - strand, within BBR_RS21170 at 2421.715 kb on - strand, within BBR_RS21170 at 2421.746 kb on - strand, within BBR_RS21170 at 2421.760 kb on - strand, within BBR_RS21170 at 2421.767 kb on - strand, within BBR_RS21170 at 2421.796 kb on - strand, within BBR_RS21170 at 2421.806 kb on - strand, within BBR_RS21170 at 2421.807 kb on - strand, within BBR_RS21170 at 2421.813 kb on - strand, within BBR_RS21170 at 2421.825 kb on - strand at 2421.835 kb on - strand at 2421.835 kb on - strand at 2421.840 kb on - strand at 2421.840 kb on - strand at 2421.840 kb on - strand at 2421.844 kb on - strand at 2421.845 kb on - strand at 2422.192 kb on - strand at 2422.192 kb on - strand at 2422.201 kb on - strand at 2422.209 kb on + strand at 2422.244 kb on + strand at 2422.443 kb on + strand at 2422.443 kb on + strand at 2422.490 kb on + strand at 2422.522 kb on - strand at 2422.523 kb on + strand at 2422.523 kb on + strand at 2422.544 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Streptomycin 0.3 mM remove 2,420,600 - -0.6 2,420,634 - BBR_RS20480 0.11 -1.8 2,421,470 - -0.7 2,421,482 - -0.9 2,421,497 - -1.1 2,421,521 - -1.9 2,421,551 - -1.8 2,421,551 - +0.7 2,421,551 - -0.8 2,421,551 - -2.3 2,421,551 - -1.5 2,421,551 - +1.0 2,421,551 - -0.5 2,421,551 - -2.0 2,421,551 - -0.4 2,421,551 - -0.4 2,421,551 - -0.5 2,421,551 - +0.6 2,421,572 - BBR_RS21170 0.14 +1.0 2,421,577 - BBR_RS21170 0.15 -0.3 2,421,577 - BBR_RS21170 0.15 -2.7 2,421,585 - BBR_RS21170 0.18 -0.3 2,421,587 - BBR_RS21170 0.18 -1.2 2,421,587 - BBR_RS21170 0.18 +0.2 2,421,587 - BBR_RS21170 0.18 -1.6 2,421,587 - BBR_RS21170 0.18 -0.8 2,421,595 - BBR_RS21170 0.21 -0.2 2,421,595 - BBR_RS21170 0.21 +0.0 2,421,630 - BBR_RS21170 0.32 -2.6 2,421,644 - BBR_RS21170 0.36 -1.4 2,421,644 - BBR_RS21170 0.36 -1.6 2,421,660 - BBR_RS21170 0.41 +0.2 2,421,660 - BBR_RS21170 0.41 -1.7 2,421,660 - BBR_RS21170 0.41 -1.0 2,421,660 - BBR_RS21170 0.41 -3.0 2,421,693 - BBR_RS21170 0.52 -0.2 2,421,699 - BBR_RS21170 0.53 -0.1 2,421,699 - BBR_RS21170 0.53 -2.8 2,421,699 - BBR_RS21170 0.53 -1.1 2,421,699 - BBR_RS21170 0.53 -3.6 2,421,699 - BBR_RS21170 0.53 -0.9 2,421,715 - BBR_RS21170 0.58 -3.8 2,421,715 - BBR_RS21170 0.58 -1.7 2,421,715 - BBR_RS21170 0.58 -1.4 2,421,715 - BBR_RS21170 0.58 -1.6 2,421,746 - BBR_RS21170 0.68 +0.2 2,421,760 - BBR_RS21170 0.73 -1.5 2,421,767 - BBR_RS21170 0.75 -3.3 2,421,796 - BBR_RS21170 0.84 -1.9 2,421,806 - BBR_RS21170 0.87 -1.3 2,421,807 - BBR_RS21170 0.87 -2.0 2,421,813 - BBR_RS21170 0.89 -2.0 2,421,825 - -0.5 2,421,835 - -2.2 2,421,835 - +0.2 2,421,840 - -1.3 2,421,840 - -2.0 2,421,840 - -0.4 2,421,844 - +0.2 2,421,845 - -0.2 2,422,192 - -1.0 2,422,192 - -1.9 2,422,201 - -1.1 2,422,209 + -1.3 2,422,244 + -0.9 2,422,443 + +1.0 2,422,443 + -0.4 2,422,490 + -0.4 2,422,522 - +0.8 2,422,523 + +0.1 2,422,523 + -0.9 2,422,544 + -2.3
Or see this region's nucleotide sequence