Experiment: Recovery broth with Streptomycin 0.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS17215 and BBR_RS17220 are separated by 63 nucleotides BBR_RS17220 and BBR_RS21000 are separated by 358 nucleotides BBR_RS21000 and BBR_RS17225 overlap by 68 nucleotides
BBR_RS17215: BBR_RS17215 - bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase, at 1,640,812 to 1,641,621
_RS17215
BBR_RS17220: BBR_RS17220 - thiamine-binding protein, at 1,641,685 to 1,642,062
_RS17220
BBR_RS21000: BBR_RS21000 - hypothetical protein, at 1,642,421 to 1,642,654
_RS21000
BBR_RS17225: BBR_RS17225 - MFS transporter, at 1,642,587 to 1,643,966
_RS17225
Position (kb)
1642
1643 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1641.485 kb on + strand, within BBR_RS17215 at 1641.513 kb on + strand, within BBR_RS17215 at 1641.513 kb on + strand, within BBR_RS17215 at 1641.513 kb on + strand, within BBR_RS17215 at 1641.521 kb on - strand, within BBR_RS17215 at 1641.521 kb on - strand, within BBR_RS17215 at 1641.521 kb on - strand, within BBR_RS17215 at 1641.553 kb on + strand at 1641.563 kb on - strand at 1641.575 kb on - strand at 1641.635 kb on - strand at 1641.643 kb on + strand at 1641.643 kb on + strand at 1641.658 kb on + strand at 1641.666 kb on - strand at 1641.688 kb on + strand at 1641.698 kb on + strand at 1641.718 kb on + strand at 1641.748 kb on + strand, within BBR_RS17220 at 1641.750 kb on + strand, within BBR_RS17220 at 1641.758 kb on - strand, within BBR_RS17220 at 1641.766 kb on + strand, within BBR_RS17220 at 1641.777 kb on - strand, within BBR_RS17220 at 1641.780 kb on + strand, within BBR_RS17220 at 1641.808 kb on - strand, within BBR_RS17220 at 1641.869 kb on - strand, within BBR_RS17220 at 1641.871 kb on + strand, within BBR_RS17220 at 1641.873 kb on + strand, within BBR_RS17220 at 1641.904 kb on + strand, within BBR_RS17220 at 1641.906 kb on + strand, within BBR_RS17220 at 1641.913 kb on - strand, within BBR_RS17220 at 1641.967 kb on - strand, within BBR_RS17220 at 1641.967 kb on - strand, within BBR_RS17220 at 1642.003 kb on - strand, within BBR_RS17220 at 1642.025 kb on - strand at 1642.026 kb on + strand at 1642.081 kb on + strand at 1642.086 kb on + strand at 1642.092 kb on + strand at 1642.145 kb on + strand at 1642.166 kb on - strand at 1642.246 kb on + strand at 1642.260 kb on - strand at 1642.306 kb on - strand at 1642.312 kb on + strand at 1642.321 kb on + strand at 1642.321 kb on + strand at 1642.349 kb on - strand at 1642.416 kb on - strand at 1642.436 kb on - strand at 1642.482 kb on + strand, within BBR_RS21000 at 1642.518 kb on - strand, within BBR_RS21000 at 1642.539 kb on + strand, within BBR_RS21000 at 1642.539 kb on + strand, within BBR_RS21000 at 1642.602 kb on - strand at 1642.639 kb on + strand at 1642.673 kb on + strand at 1642.675 kb on - strand at 1642.752 kb on + strand, within BBR_RS17225 at 1642.774 kb on + strand, within BBR_RS17225 at 1642.774 kb on + strand, within BBR_RS17225 at 1642.822 kb on + strand, within BBR_RS17225 at 1642.822 kb on + strand, within BBR_RS17225 at 1642.830 kb on - strand, within BBR_RS17225 at 1642.853 kb on - strand, within BBR_RS17225 at 1642.903 kb on + strand, within BBR_RS17225 at 1642.916 kb on - strand, within BBR_RS17225 at 1642.988 kb on + strand, within BBR_RS17225 at 1642.988 kb on + strand, within BBR_RS17225 at 1642.999 kb on + strand, within BBR_RS17225 at 1643.003 kb on + strand, within BBR_RS17225 at 1643.097 kb on + strand, within BBR_RS17225 at 1643.105 kb on - strand, within BBR_RS17225 at 1643.110 kb on + strand, within BBR_RS17225 at 1643.129 kb on + strand, within BBR_RS17225 at 1643.151 kb on + strand, within BBR_RS17225 at 1643.151 kb on + strand, within BBR_RS17225 at 1643.151 kb on + strand, within BBR_RS17225 at 1643.156 kb on + strand, within BBR_RS17225 at 1643.159 kb on + strand, within BBR_RS17225 at 1643.159 kb on - strand, within BBR_RS17225 at 1643.159 kb on - strand, within BBR_RS17225 at 1643.161 kb on - strand, within BBR_RS17225 at 1643.169 kb on - strand, within BBR_RS17225 at 1643.183 kb on - strand, within BBR_RS17225 at 1643.196 kb on - strand, within BBR_RS17225 at 1643.206 kb on - strand, within BBR_RS17225 at 1643.206 kb on - strand, within BBR_RS17225 at 1643.220 kb on - strand, within BBR_RS17225 at 1643.236 kb on - strand, within BBR_RS17225 at 1643.254 kb on + strand, within BBR_RS17225 at 1643.277 kb on + strand, within BBR_RS17225 at 1643.285 kb on - strand, within BBR_RS17225 at 1643.318 kb on + strand, within BBR_RS17225 at 1643.332 kb on + strand, within BBR_RS17225 at 1643.332 kb on + strand, within BBR_RS17225 at 1643.340 kb on - strand, within BBR_RS17225 at 1643.340 kb on - strand, within BBR_RS17225 at 1643.340 kb on - strand, within BBR_RS17225 at 1643.342 kb on + strand, within BBR_RS17225 at 1643.422 kb on - strand, within BBR_RS17225 at 1643.453 kb on + strand, within BBR_RS17225 at 1643.455 kb on + strand, within BBR_RS17225 at 1643.455 kb on - strand, within BBR_RS17225 at 1643.492 kb on - strand, within BBR_RS17225 at 1643.500 kb on + strand, within BBR_RS17225 at 1643.500 kb on + strand, within BBR_RS17225 at 1643.500 kb on + strand, within BBR_RS17225 at 1643.500 kb on + strand, within BBR_RS17225 at 1643.500 kb on + strand, within BBR_RS17225 at 1643.500 kb on + strand, within BBR_RS17225 at 1643.500 kb on + strand, within BBR_RS17225 at 1643.500 kb on + strand, within BBR_RS17225 at 1643.500 kb on + strand, within BBR_RS17225 at 1643.508 kb on - strand, within BBR_RS17225 at 1643.508 kb on - strand, within BBR_RS17225 at 1643.508 kb on - strand, within BBR_RS17225 at 1643.518 kb on - strand, within BBR_RS17225 at 1643.546 kb on - strand, within BBR_RS17225 at 1643.580 kb on - strand, within BBR_RS17225 at 1643.648 kb on + strand, within BBR_RS17225 at 1643.651 kb on - strand, within BBR_RS17225
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Streptomycin 0.3 mM remove 1,641,485 + BBR_RS17215 0.83 +0.6 1,641,513 + BBR_RS17215 0.87 -0.4 1,641,513 + BBR_RS17215 0.87 +0.5 1,641,513 + BBR_RS17215 0.87 -0.8 1,641,521 - BBR_RS17215 0.88 +0.4 1,641,521 - BBR_RS17215 0.88 +0.1 1,641,521 - BBR_RS17215 0.88 +0.3 1,641,553 + -0.1 1,641,563 - +1.1 1,641,575 - +0.4 1,641,635 - +1.7 1,641,643 + -0.4 1,641,643 + +0.2 1,641,658 + -0.1 1,641,666 - +0.1 1,641,688 + +0.8 1,641,698 + +2.1 1,641,718 + -0.6 1,641,748 + BBR_RS17220 0.17 +1.7 1,641,750 + BBR_RS17220 0.17 -1.6 1,641,758 - BBR_RS17220 0.19 -0.9 1,641,766 + BBR_RS17220 0.21 -0.6 1,641,777 - BBR_RS17220 0.24 +1.4 1,641,780 + BBR_RS17220 0.25 +1.9 1,641,808 - BBR_RS17220 0.33 -1.5 1,641,869 - BBR_RS17220 0.49 +1.2 1,641,871 + BBR_RS17220 0.49 -0.5 1,641,873 + BBR_RS17220 0.50 -0.3 1,641,904 + BBR_RS17220 0.58 -0.7 1,641,906 + BBR_RS17220 0.58 +0.2 1,641,913 - BBR_RS17220 0.60 +0.1 1,641,967 - BBR_RS17220 0.75 +1.5 1,641,967 - BBR_RS17220 0.75 +0.1 1,642,003 - BBR_RS17220 0.84 -0.1 1,642,025 - +0.8 1,642,026 + +0.3 1,642,081 + -0.0 1,642,086 + +0.8 1,642,092 + +1.1 1,642,145 + +0.5 1,642,166 - -0.0 1,642,246 + +2.6 1,642,260 - +0.1 1,642,306 - +0.1 1,642,312 + -2.5 1,642,321 + -0.9 1,642,321 + +0.8 1,642,349 - -0.5 1,642,416 - -1.5 1,642,436 - +1.0 1,642,482 + BBR_RS21000 0.26 -0.0 1,642,518 - BBR_RS21000 0.41 -1.6 1,642,539 + BBR_RS21000 0.50 -0.3 1,642,539 + BBR_RS21000 0.50 +0.9 1,642,602 - -0.4 1,642,639 + +1.1 1,642,673 + -0.7 1,642,675 - +0.1 1,642,752 + BBR_RS17225 0.12 -2.2 1,642,774 + BBR_RS17225 0.14 +0.6 1,642,774 + BBR_RS17225 0.14 +1.1 1,642,822 + BBR_RS17225 0.17 +0.6 1,642,822 + BBR_RS17225 0.17 +0.3 1,642,830 - BBR_RS17225 0.18 +1.3 1,642,853 - BBR_RS17225 0.19 +0.4 1,642,903 + BBR_RS17225 0.23 -0.7 1,642,916 - BBR_RS17225 0.24 +0.3 1,642,988 + BBR_RS17225 0.29 +0.4 1,642,988 + BBR_RS17225 0.29 -0.5 1,642,999 + BBR_RS17225 0.30 -0.6 1,643,003 + BBR_RS17225 0.30 +0.9 1,643,097 + BBR_RS17225 0.37 -0.1 1,643,105 - BBR_RS17225 0.38 -0.2 1,643,110 + BBR_RS17225 0.38 +1.5 1,643,129 + BBR_RS17225 0.39 +0.4 1,643,151 + BBR_RS17225 0.41 +0.8 1,643,151 + BBR_RS17225 0.41 +1.6 1,643,151 + BBR_RS17225 0.41 -2.2 1,643,156 + BBR_RS17225 0.41 +0.4 1,643,159 + BBR_RS17225 0.41 +0.3 1,643,159 - BBR_RS17225 0.41 -0.7 1,643,159 - BBR_RS17225 0.41 -0.6 1,643,161 - BBR_RS17225 0.42 +0.3 1,643,169 - BBR_RS17225 0.42 -0.5 1,643,183 - BBR_RS17225 0.43 +1.5 1,643,196 - BBR_RS17225 0.44 -0.5 1,643,206 - BBR_RS17225 0.45 -0.7 1,643,206 - BBR_RS17225 0.45 -0.0 1,643,220 - BBR_RS17225 0.46 +2.1 1,643,236 - BBR_RS17225 0.47 +0.0 1,643,254 + BBR_RS17225 0.48 +1.1 1,643,277 + BBR_RS17225 0.50 -0.6 1,643,285 - BBR_RS17225 0.51 -0.0 1,643,318 + BBR_RS17225 0.53 +0.0 1,643,332 + BBR_RS17225 0.54 -0.7 1,643,332 + BBR_RS17225 0.54 -0.0 1,643,340 - BBR_RS17225 0.55 +0.1 1,643,340 - BBR_RS17225 0.55 -0.1 1,643,340 - BBR_RS17225 0.55 +0.2 1,643,342 + BBR_RS17225 0.55 -0.9 1,643,422 - BBR_RS17225 0.61 -0.2 1,643,453 + BBR_RS17225 0.63 -2.2 1,643,455 + BBR_RS17225 0.63 +0.1 1,643,455 - BBR_RS17225 0.63 -0.9 1,643,492 - BBR_RS17225 0.66 +0.4 1,643,500 + BBR_RS17225 0.66 +0.5 1,643,500 + BBR_RS17225 0.66 -0.6 1,643,500 + BBR_RS17225 0.66 +0.0 1,643,500 + BBR_RS17225 0.66 -1.0 1,643,500 + BBR_RS17225 0.66 -1.2 1,643,500 + BBR_RS17225 0.66 +0.9 1,643,500 + BBR_RS17225 0.66 -0.5 1,643,500 + BBR_RS17225 0.66 -1.1 1,643,500 + BBR_RS17225 0.66 +0.4 1,643,508 - BBR_RS17225 0.67 -0.0 1,643,508 - BBR_RS17225 0.67 +0.4 1,643,508 - BBR_RS17225 0.67 -0.3 1,643,518 - BBR_RS17225 0.67 +2.4 1,643,546 - BBR_RS17225 0.69 -1.9 1,643,580 - BBR_RS17225 0.72 -2.1 1,643,648 + BBR_RS17225 0.77 -1.2 1,643,651 - BBR_RS17225 0.77 -1.5
Or see this region's nucleotide sequence