Experiment: Recovery broth with Streptomycin 0.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS17645 and BBR_RS17650 are separated by 184 nucleotides BBR_RS17650 and BBR_RS17655 are separated by 442 nucleotides
BBR_RS17645: BBR_RS17645 - methionine adenosyltransferase, at 1,736,795 to 1,738,015
_RS17645
BBR_RS17650: BBR_RS17650 - cell filamentation protein Fic, at 1,738,200 to 1,739,012
_RS17650
BBR_RS17655: BBR_RS17655 - transposase, at 1,739,455 to 1,740,216
_RS17655
Position (kb)
1738
1739
1740 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1737.996 kb on - strand at 1737.996 kb on - strand at 1737.996 kb on - strand at 1737.996 kb on - strand at 1738.004 kb on + strand at 1738.009 kb on + strand at 1738.043 kb on - strand at 1738.226 kb on + strand at 1738.268 kb on - strand at 1738.399 kb on - strand, within BBR_RS17650 at 1738.466 kb on - strand, within BBR_RS17650 at 1738.800 kb on + strand, within BBR_RS17650 at 1738.800 kb on + strand, within BBR_RS17650 at 1738.943 kb on + strand at 1738.948 kb on + strand at 1738.948 kb on + strand at 1738.994 kb on + strand at 1738.994 kb on + strand at 1739.006 kb on + strand at 1739.019 kb on + strand at 1739.019 kb on + strand at 1739.019 kb on - strand at 1739.028 kb on - strand at 1739.031 kb on + strand at 1739.066 kb on + strand at 1739.079 kb on - strand at 1739.098 kb on - strand at 1739.105 kb on + strand at 1739.105 kb on + strand at 1739.105 kb on + strand at 1739.107 kb on + strand at 1739.107 kb on + strand at 1739.107 kb on + strand at 1739.113 kb on - strand at 1739.113 kb on - strand at 1739.113 kb on - strand at 1739.162 kb on + strand at 1739.170 kb on - strand at 1739.170 kb on - strand at 1739.211 kb on - strand at 1739.293 kb on + strand at 1739.295 kb on + strand at 1739.309 kb on - strand at 1739.319 kb on - strand at 1739.349 kb on - strand at 1739.349 kb on - strand at 1739.364 kb on - strand at 1739.435 kb on + strand at 1739.460 kb on - strand at 1739.460 kb on - strand at 1739.479 kb on + strand at 1739.484 kb on + strand at 1739.487 kb on - strand at 1739.502 kb on + strand at 1739.541 kb on - strand, within BBR_RS17655 at 1739.556 kb on - strand, within BBR_RS17655 at 1739.645 kb on - strand, within BBR_RS17655 at 1739.645 kb on - strand, within BBR_RS17655 at 1739.717 kb on - strand, within BBR_RS17655 at 1739.724 kb on + strand, within BBR_RS17655 at 1739.741 kb on + strand, within BBR_RS17655 at 1739.742 kb on - strand, within BBR_RS17655 at 1739.749 kb on - strand, within BBR_RS17655 at 1739.763 kb on - strand, within BBR_RS17655 at 1739.792 kb on + strand, within BBR_RS17655 at 1739.811 kb on + strand, within BBR_RS17655 at 1739.825 kb on + strand, within BBR_RS17655 at 1739.825 kb on + strand, within BBR_RS17655 at 1739.833 kb on - strand, within BBR_RS17655 at 1739.835 kb on - strand, within BBR_RS17655 at 1739.854 kb on - strand, within BBR_RS17655 at 1739.899 kb on + strand, within BBR_RS17655 at 1739.900 kb on + strand, within BBR_RS17655 at 1739.908 kb on + strand, within BBR_RS17655 at 1739.941 kb on + strand, within BBR_RS17655 at 1739.946 kb on + strand, within BBR_RS17655 at 1739.965 kb on + strand, within BBR_RS17655 at 1739.985 kb on + strand, within BBR_RS17655 at 1739.994 kb on - strand, within BBR_RS17655
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Streptomycin 0.3 mM remove 1,737,996 - +1.1 1,737,996 - +1.6 1,737,996 - +1.5 1,737,996 - -0.7 1,738,004 + +0.7 1,738,009 + -0.3 1,738,043 - -2.1 1,738,226 + +0.8 1,738,268 - +0.7 1,738,399 - BBR_RS17650 0.24 -0.5 1,738,466 - BBR_RS17650 0.33 -0.9 1,738,800 + BBR_RS17650 0.74 +0.2 1,738,800 + BBR_RS17650 0.74 -0.3 1,738,943 + -0.4 1,738,948 + +0.7 1,738,948 + -1.1 1,738,994 + -1.4 1,738,994 + +0.1 1,739,006 + -0.3 1,739,019 + -1.6 1,739,019 + +1.5 1,739,019 - +0.8 1,739,028 - -0.2 1,739,031 + -2.0 1,739,066 + +1.1 1,739,079 - +1.0 1,739,098 - +1.1 1,739,105 + -1.1 1,739,105 + +0.0 1,739,105 + +0.6 1,739,107 + +0.4 1,739,107 + +0.4 1,739,107 + -0.6 1,739,113 - +0.3 1,739,113 - +2.5 1,739,113 - -1.4 1,739,162 + -0.1 1,739,170 - -2.8 1,739,170 - +0.4 1,739,211 - +0.4 1,739,293 + -1.2 1,739,295 + -2.5 1,739,309 - +0.3 1,739,319 - +0.9 1,739,349 - -0.3 1,739,349 - +0.8 1,739,364 - +1.8 1,739,435 + +0.5 1,739,460 - -0.2 1,739,460 - +0.5 1,739,479 + -0.2 1,739,484 + +0.4 1,739,487 - -1.4 1,739,502 + +0.2 1,739,541 - BBR_RS17655 0.11 -1.7 1,739,556 - BBR_RS17655 0.13 -0.5 1,739,645 - BBR_RS17655 0.25 -0.0 1,739,645 - BBR_RS17655 0.25 +0.3 1,739,717 - BBR_RS17655 0.34 -0.8 1,739,724 + BBR_RS17655 0.35 -0.6 1,739,741 + BBR_RS17655 0.38 +1.0 1,739,742 - BBR_RS17655 0.38 +0.7 1,739,749 - BBR_RS17655 0.39 +0.9 1,739,763 - BBR_RS17655 0.40 +0.5 1,739,792 + BBR_RS17655 0.44 +1.1 1,739,811 + BBR_RS17655 0.47 +0.9 1,739,825 + BBR_RS17655 0.49 -0.2 1,739,825 + BBR_RS17655 0.49 -0.9 1,739,833 - BBR_RS17655 0.50 -0.2 1,739,835 - BBR_RS17655 0.50 +0.3 1,739,854 - BBR_RS17655 0.52 +0.5 1,739,899 + BBR_RS17655 0.58 +0.1 1,739,900 + BBR_RS17655 0.58 +0.3 1,739,908 + BBR_RS17655 0.59 +0.2 1,739,941 + BBR_RS17655 0.64 +0.5 1,739,946 + BBR_RS17655 0.64 +1.2 1,739,965 + BBR_RS17655 0.67 +0.1 1,739,985 + BBR_RS17655 0.70 -1.2 1,739,994 - BBR_RS17655 0.71 +0.5
Or see this region's nucleotide sequence