Experiment: Recovery broth with Streptomycin 0.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS17635 and BBR_RS17640 are separated by 166 nucleotides BBR_RS17640 and BBR_RS17645 are separated by 285 nucleotides
BBR_RS17635: BBR_RS17635 - dihydroxy-acid dehydratase, at 1,734,196 to 1,736,058
_RS17635
BBR_RS17640: BBR_RS17640 - DNA-directed RNA polymerase subunit omega, at 1,736,225 to 1,736,509
_RS17640
BBR_RS17645: BBR_RS17645 - methionine adenosyltransferase, at 1,736,795 to 1,738,015
_RS17645
Position (kb)
1736
1737 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1735.225 kb on + strand, within BBR_RS17635 at 1735.226 kb on + strand, within BBR_RS17635 at 1735.226 kb on + strand, within BBR_RS17635 at 1735.233 kb on - strand, within BBR_RS17635 at 1735.233 kb on - strand, within BBR_RS17635 at 1735.234 kb on - strand, within BBR_RS17635 at 1735.234 kb on - strand, within BBR_RS17635 at 1735.234 kb on - strand, within BBR_RS17635 at 1735.236 kb on + strand, within BBR_RS17635 at 1735.244 kb on - strand, within BBR_RS17635 at 1735.244 kb on - strand, within BBR_RS17635 at 1735.244 kb on - strand, within BBR_RS17635 at 1735.252 kb on - strand, within BBR_RS17635 at 1735.307 kb on - strand, within BBR_RS17635 at 1735.321 kb on - strand, within BBR_RS17635 at 1735.360 kb on - strand, within BBR_RS17635 at 1735.360 kb on - strand, within BBR_RS17635 at 1735.369 kb on - strand, within BBR_RS17635 at 1735.380 kb on - strand, within BBR_RS17635 at 1735.382 kb on - strand, within BBR_RS17635 at 1735.397 kb on - strand, within BBR_RS17635 at 1735.410 kb on + strand, within BBR_RS17635 at 1735.418 kb on - strand, within BBR_RS17635 at 1735.418 kb on - strand, within BBR_RS17635 at 1735.456 kb on + strand, within BBR_RS17635 at 1735.456 kb on + strand, within BBR_RS17635 at 1735.464 kb on - strand, within BBR_RS17635 at 1735.473 kb on + strand, within BBR_RS17635 at 1735.504 kb on + strand, within BBR_RS17635 at 1735.546 kb on + strand, within BBR_RS17635 at 1735.549 kb on - strand, within BBR_RS17635 at 1735.552 kb on - strand, within BBR_RS17635 at 1735.557 kb on - strand, within BBR_RS17635 at 1735.563 kb on + strand, within BBR_RS17635 at 1735.570 kb on + strand, within BBR_RS17635 at 1735.571 kb on - strand, within BBR_RS17635 at 1735.578 kb on - strand, within BBR_RS17635 at 1735.588 kb on + strand, within BBR_RS17635 at 1735.604 kb on - strand, within BBR_RS17635 at 1735.609 kb on - strand, within BBR_RS17635 at 1735.654 kb on - strand, within BBR_RS17635 at 1735.656 kb on + strand, within BBR_RS17635 at 1735.658 kb on - strand, within BBR_RS17635 at 1735.661 kb on + strand, within BBR_RS17635 at 1735.667 kb on + strand, within BBR_RS17635 at 1735.676 kb on + strand, within BBR_RS17635 at 1735.691 kb on - strand, within BBR_RS17635 at 1735.713 kb on + strand, within BBR_RS17635 at 1735.721 kb on - strand, within BBR_RS17635 at 1735.732 kb on + strand, within BBR_RS17635 at 1735.735 kb on - strand, within BBR_RS17635 at 1735.756 kb on - strand, within BBR_RS17635 at 1735.778 kb on + strand, within BBR_RS17635 at 1735.778 kb on + strand, within BBR_RS17635 at 1735.778 kb on + strand, within BBR_RS17635 at 1735.781 kb on - strand, within BBR_RS17635 at 1735.781 kb on - strand, within BBR_RS17635 at 1735.781 kb on - strand, within BBR_RS17635 at 1735.786 kb on - strand, within BBR_RS17635 at 1735.786 kb on - strand, within BBR_RS17635 at 1735.793 kb on + strand, within BBR_RS17635 at 1735.806 kb on - strand, within BBR_RS17635 at 1735.829 kb on + strand, within BBR_RS17635 at 1735.845 kb on + strand, within BBR_RS17635 at 1735.848 kb on - strand, within BBR_RS17635 at 1735.889 kb on + strand at 1735.921 kb on - strand at 1735.921 kb on - strand at 1735.921 kb on - strand at 1735.921 kb on - strand at 1735.931 kb on + strand at 1735.980 kb on + strand at 1735.988 kb on - strand at 1736.001 kb on - strand at 1736.006 kb on - strand at 1736.068 kb on - strand at 1736.068 kb on - strand at 1736.073 kb on - strand at 1736.085 kb on + strand at 1736.090 kb on - strand at 1736.173 kb on + strand at 1736.248 kb on - strand at 1736.373 kb on - strand, within BBR_RS17640 at 1736.373 kb on - strand, within BBR_RS17640 at 1736.408 kb on + strand, within BBR_RS17640 at 1736.410 kb on + strand, within BBR_RS17640 at 1736.431 kb on + strand, within BBR_RS17640 at 1736.463 kb on + strand, within BBR_RS17640 at 1736.532 kb on + strand at 1736.533 kb on - strand at 1736.533 kb on - strand at 1736.576 kb on + strand at 1736.674 kb on + strand at 1736.679 kb on + strand at 1736.689 kb on + strand at 1736.701 kb on + strand at 1736.711 kb on - strand at 1736.744 kb on + strand at 1736.764 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Streptomycin 0.3 mM remove 1,735,225 + BBR_RS17635 0.55 +0.5 1,735,226 + BBR_RS17635 0.55 -1.1 1,735,226 + BBR_RS17635 0.55 -0.6 1,735,233 - BBR_RS17635 0.56 +0.5 1,735,233 - BBR_RS17635 0.56 +2.1 1,735,234 - BBR_RS17635 0.56 +0.1 1,735,234 - BBR_RS17635 0.56 -0.8 1,735,234 - BBR_RS17635 0.56 -0.8 1,735,236 + BBR_RS17635 0.56 +0.6 1,735,244 - BBR_RS17635 0.56 -0.3 1,735,244 - BBR_RS17635 0.56 +0.6 1,735,244 - BBR_RS17635 0.56 -1.2 1,735,252 - BBR_RS17635 0.57 -1.5 1,735,307 - BBR_RS17635 0.60 -1.7 1,735,321 - BBR_RS17635 0.60 +0.7 1,735,360 - BBR_RS17635 0.62 +1.8 1,735,360 - BBR_RS17635 0.62 -0.8 1,735,369 - BBR_RS17635 0.63 +0.7 1,735,380 - BBR_RS17635 0.64 -1.6 1,735,382 - BBR_RS17635 0.64 -0.2 1,735,397 - BBR_RS17635 0.64 -0.3 1,735,410 + BBR_RS17635 0.65 +0.1 1,735,418 - BBR_RS17635 0.66 +2.7 1,735,418 - BBR_RS17635 0.66 -0.9 1,735,456 + BBR_RS17635 0.68 +0.4 1,735,456 + BBR_RS17635 0.68 -0.4 1,735,464 - BBR_RS17635 0.68 +3.4 1,735,473 + BBR_RS17635 0.69 -0.1 1,735,504 + BBR_RS17635 0.70 +0.6 1,735,546 + BBR_RS17635 0.72 +1.0 1,735,549 - BBR_RS17635 0.73 +1.0 1,735,552 - BBR_RS17635 0.73 +1.5 1,735,557 - BBR_RS17635 0.73 +3.8 1,735,563 + BBR_RS17635 0.73 +0.1 1,735,570 + BBR_RS17635 0.74 +0.3 1,735,571 - BBR_RS17635 0.74 -1.9 1,735,578 - BBR_RS17635 0.74 -1.8 1,735,588 + BBR_RS17635 0.75 -2.5 1,735,604 - BBR_RS17635 0.76 +1.1 1,735,609 - BBR_RS17635 0.76 -0.5 1,735,654 - BBR_RS17635 0.78 +1.9 1,735,656 + BBR_RS17635 0.78 +0.7 1,735,658 - BBR_RS17635 0.78 -0.5 1,735,661 + BBR_RS17635 0.79 -2.0 1,735,667 + BBR_RS17635 0.79 -0.3 1,735,676 + BBR_RS17635 0.79 +0.8 1,735,691 - BBR_RS17635 0.80 +0.4 1,735,713 + BBR_RS17635 0.81 +0.8 1,735,721 - BBR_RS17635 0.82 -0.9 1,735,732 + BBR_RS17635 0.82 -1.0 1,735,735 - BBR_RS17635 0.83 +1.5 1,735,756 - BBR_RS17635 0.84 -0.1 1,735,778 + BBR_RS17635 0.85 -0.1 1,735,778 + BBR_RS17635 0.85 +1.7 1,735,778 + BBR_RS17635 0.85 -0.8 1,735,781 - BBR_RS17635 0.85 -0.9 1,735,781 - BBR_RS17635 0.85 -0.9 1,735,781 - BBR_RS17635 0.85 +1.2 1,735,786 - BBR_RS17635 0.85 +0.3 1,735,786 - BBR_RS17635 0.85 +1.6 1,735,793 + BBR_RS17635 0.86 +1.8 1,735,806 - BBR_RS17635 0.86 -0.3 1,735,829 + BBR_RS17635 0.88 +0.6 1,735,845 + BBR_RS17635 0.89 -1.9 1,735,848 - BBR_RS17635 0.89 +0.5 1,735,889 + -2.0 1,735,921 - +0.5 1,735,921 - -0.7 1,735,921 - -0.7 1,735,921 - -1.8 1,735,931 + -0.3 1,735,980 + +1.7 1,735,988 - -0.3 1,736,001 - -1.9 1,736,006 - -1.7 1,736,068 - +4.0 1,736,068 - -1.6 1,736,073 - -2.0 1,736,085 + +0.2 1,736,090 - -0.5 1,736,173 + -0.5 1,736,248 - -0.9 1,736,373 - BBR_RS17640 0.52 -0.3 1,736,373 - BBR_RS17640 0.52 -0.4 1,736,408 + BBR_RS17640 0.64 -1.0 1,736,410 + BBR_RS17640 0.65 +0.8 1,736,431 + BBR_RS17640 0.72 -1.7 1,736,463 + BBR_RS17640 0.84 +2.0 1,736,532 + +0.7 1,736,533 - -2.0 1,736,533 - +0.8 1,736,576 + +0.1 1,736,674 + -0.3 1,736,679 + -0.7 1,736,689 + +1.5 1,736,701 + +0.6 1,736,711 - +0.4 1,736,744 + +0.5 1,736,764 - -0.2
Or see this region's nucleotide sequence