Experiment: Recovery broth with Streptomycin 0.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS16840 and BBR_RS16845 are separated by 44 nucleotides BBR_RS16845 and BBR_RS16850 are separated by 570 nucleotides
BBR_RS16840: BBR_RS16840 - chromosome segregation protein SMC, at 1,546,370 to 1,550,017
_RS16840
BBR_RS16845: BBR_RS16845 - bifunctional folylpolyglutamate synthase/dihydrofolate synthase, at 1,550,062 to 1,551,663
_RS16845
BBR_RS16850: BBR_RS16850 - peptidase M24 family protein, at 1,552,234 to 1,553,829
_RS16850
Position (kb)
1550
1551
1552 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1549.105 kb on + strand, within BBR_RS16840 at 1549.113 kb on - strand, within BBR_RS16840 at 1549.218 kb on + strand, within BBR_RS16840 at 1549.220 kb on - strand, within BBR_RS16840 at 1549.225 kb on - strand, within BBR_RS16840 at 1549.226 kb on - strand, within BBR_RS16840 at 1549.226 kb on - strand, within BBR_RS16840 at 1549.226 kb on - strand, within BBR_RS16840 at 1549.226 kb on - strand, within BBR_RS16840 at 1549.228 kb on + strand, within BBR_RS16840 at 1549.228 kb on + strand, within BBR_RS16840 at 1549.251 kb on - strand, within BBR_RS16840 at 1549.253 kb on + strand, within BBR_RS16840 at 1549.321 kb on + strand, within BBR_RS16840 at 1549.347 kb on - strand, within BBR_RS16840 at 1549.361 kb on - strand, within BBR_RS16840 at 1549.475 kb on - strand, within BBR_RS16840 at 1549.484 kb on - strand, within BBR_RS16840 at 1549.508 kb on - strand, within BBR_RS16840 at 1549.508 kb on - strand, within BBR_RS16840 at 1549.510 kb on + strand, within BBR_RS16840 at 1549.558 kb on + strand, within BBR_RS16840 at 1549.790 kb on + strand at 1549.790 kb on + strand at 1549.792 kb on + strand at 1549.798 kb on - strand at 1549.798 kb on - strand at 1549.800 kb on - strand at 1549.842 kb on - strand at 1549.842 kb on - strand at 1549.853 kb on - strand at 1549.865 kb on + strand at 1549.865 kb on + strand at 1549.878 kb on + strand at 1549.886 kb on - strand at 1549.886 kb on - strand at 1549.944 kb on - strand at 1550.026 kb on - strand at 1550.042 kb on + strand at 1550.042 kb on + strand at 1550.050 kb on - strand at 1550.063 kb on - strand at 1550.081 kb on + strand at 1550.094 kb on - strand at 1550.094 kb on - strand at 1550.124 kb on + strand at 1550.130 kb on + strand at 1550.168 kb on + strand at 1550.176 kb on - strand at 1550.195 kb on + strand at 1550.213 kb on - strand at 1550.258 kb on + strand, within BBR_RS16845 at 1550.258 kb on + strand, within BBR_RS16845 at 1550.274 kb on + strand, within BBR_RS16845 at 1550.279 kb on + strand, within BBR_RS16845 at 1551.607 kb on - strand at 1551.607 kb on - strand at 1551.677 kb on - strand at 1551.677 kb on - strand at 1551.677 kb on - strand at 1551.858 kb on - strand at 1551.860 kb on + strand at 1551.868 kb on - strand at 1551.868 kb on - strand at 1551.868 kb on - strand at 1551.878 kb on - strand at 1551.900 kb on - strand at 1551.900 kb on - strand at 1551.906 kb on + strand at 1552.098 kb on - strand at 1552.103 kb on + strand at 1552.105 kb on + strand at 1552.111 kb on - strand at 1552.113 kb on - strand at 1552.216 kb on + strand at 1552.216 kb on + strand at 1552.223 kb on + strand at 1552.224 kb on - strand at 1552.224 kb on - strand at 1552.224 kb on - strand at 1552.281 kb on + strand at 1552.308 kb on + strand at 1552.316 kb on - strand at 1552.500 kb on + strand, within BBR_RS16850 at 1552.509 kb on + strand, within BBR_RS16850 at 1552.509 kb on + strand, within BBR_RS16850 at 1552.567 kb on + strand, within BBR_RS16850 at 1552.567 kb on + strand, within BBR_RS16850 at 1552.575 kb on - strand, within BBR_RS16850 at 1552.575 kb on - strand, within BBR_RS16850 at 1552.575 kb on - strand, within BBR_RS16850 at 1552.652 kb on - strand, within BBR_RS16850 at 1552.652 kb on - strand, within BBR_RS16850
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Streptomycin 0.3 mM remove 1,549,105 + BBR_RS16840 0.75 -0.1 1,549,113 - BBR_RS16840 0.75 -0.4 1,549,218 + BBR_RS16840 0.78 -0.8 1,549,220 - BBR_RS16840 0.78 -1.0 1,549,225 - BBR_RS16840 0.78 +0.6 1,549,226 - BBR_RS16840 0.78 -0.6 1,549,226 - BBR_RS16840 0.78 +0.1 1,549,226 - BBR_RS16840 0.78 -0.1 1,549,226 - BBR_RS16840 0.78 -0.1 1,549,228 + BBR_RS16840 0.78 -1.0 1,549,228 + BBR_RS16840 0.78 -0.5 1,549,251 - BBR_RS16840 0.79 -0.4 1,549,253 + BBR_RS16840 0.79 -1.1 1,549,321 + BBR_RS16840 0.81 -0.4 1,549,347 - BBR_RS16840 0.82 -0.3 1,549,361 - BBR_RS16840 0.82 -1.9 1,549,475 - BBR_RS16840 0.85 +0.4 1,549,484 - BBR_RS16840 0.85 +0.6 1,549,508 - BBR_RS16840 0.86 +2.8 1,549,508 - BBR_RS16840 0.86 +0.5 1,549,510 + BBR_RS16840 0.86 -1.3 1,549,558 + BBR_RS16840 0.87 +0.3 1,549,790 + -0.9 1,549,790 + +1.1 1,549,792 + -0.4 1,549,798 - +1.6 1,549,798 - -0.7 1,549,800 - -0.3 1,549,842 - +0.6 1,549,842 - -1.1 1,549,853 - +0.2 1,549,865 + +0.6 1,549,865 + -0.9 1,549,878 + +1.0 1,549,886 - +0.6 1,549,886 - -0.9 1,549,944 - -0.1 1,550,026 - -2.0 1,550,042 + -0.9 1,550,042 + +1.5 1,550,050 - -0.4 1,550,063 - -0.1 1,550,081 + +1.3 1,550,094 - +1.4 1,550,094 - -1.4 1,550,124 + +1.4 1,550,130 + +2.0 1,550,168 + +0.8 1,550,176 - +1.3 1,550,195 + -1.7 1,550,213 - -0.5 1,550,258 + BBR_RS16845 0.12 -0.6 1,550,258 + BBR_RS16845 0.12 +0.7 1,550,274 + BBR_RS16845 0.13 -0.1 1,550,279 + BBR_RS16845 0.14 -0.0 1,551,607 - -0.5 1,551,607 - +0.2 1,551,677 - +2.1 1,551,677 - -0.6 1,551,677 - +0.4 1,551,858 - -0.3 1,551,860 + +0.9 1,551,868 - -1.7 1,551,868 - +0.4 1,551,868 - +0.1 1,551,878 - +0.2 1,551,900 - -1.9 1,551,900 - +1.1 1,551,906 + +0.6 1,552,098 - +1.7 1,552,103 + -1.5 1,552,105 + +1.6 1,552,111 - +0.4 1,552,113 - +0.8 1,552,216 + +0.2 1,552,216 + +2.0 1,552,223 + +1.1 1,552,224 - +1.8 1,552,224 - -0.2 1,552,224 - -0.7 1,552,281 + +1.7 1,552,308 + +0.1 1,552,316 - +0.9 1,552,500 + BBR_RS16850 0.17 -0.7 1,552,509 + BBR_RS16850 0.17 +1.0 1,552,509 + BBR_RS16850 0.17 +0.6 1,552,567 + BBR_RS16850 0.21 +0.7 1,552,567 + BBR_RS16850 0.21 +0.9 1,552,575 - BBR_RS16850 0.21 -1.5 1,552,575 - BBR_RS16850 0.21 -2.1 1,552,575 - BBR_RS16850 0.21 -1.2 1,552,652 - BBR_RS16850 0.26 -0.6 1,552,652 - BBR_RS16850 0.26 -0.3
Or see this region's nucleotide sequence