Experiment: Recovery broth with Streptomycin 0.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS14480 and BBR_RS14485 are separated by 181 nucleotides BBR_RS14485 and BBR_RS14490 overlap by 4 nucleotides BBR_RS14490 and BBR_RS14495 are separated by 54 nucleotides
BBR_RS14480: BBR_RS14480 - pyruvate formate-lyase 1-activating enzyme, at 1,023,478 to 1,024,359
_RS14480
BBR_RS14485: BBR_RS14485 - DUF3000 domain-containing protein, at 1,024,541 to 1,025,173
_RS14485
BBR_RS14490: BBR_RS14490 - ribonuclease D, at 1,025,170 to 1,026,471
_RS14490
BBR_RS14495: BBR_RS14495 - trigger factor, at 1,026,526 to 1,027,899
_RS14495
Position (kb)
1025
1026
1027 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1024.178 kb on - strand, within BBR_RS14480 at 1024.250 kb on + strand, within BBR_RS14480 at 1024.258 kb on - strand, within BBR_RS14480 at 1024.299 kb on - strand at 1024.349 kb on - strand at 1024.361 kb on - strand at 1024.383 kb on - strand at 1024.391 kb on - strand at 1024.437 kb on - strand at 1024.444 kb on + strand at 1024.494 kb on - strand at 1024.569 kb on - strand at 1024.569 kb on - strand at 1024.595 kb on + strand at 1024.605 kb on - strand, within BBR_RS14485 at 1024.617 kb on + strand, within BBR_RS14485 at 1024.617 kb on + strand, within BBR_RS14485 at 1024.617 kb on + strand, within BBR_RS14485 at 1024.692 kb on + strand, within BBR_RS14485 at 1024.699 kb on - strand, within BBR_RS14485 at 1024.726 kb on + strand, within BBR_RS14485 at 1024.838 kb on + strand, within BBR_RS14485 at 1024.905 kb on + strand, within BBR_RS14485 at 1024.994 kb on - strand, within BBR_RS14485 at 1024.999 kb on - strand, within BBR_RS14485 at 1025.071 kb on - strand, within BBR_RS14485 at 1025.071 kb on - strand, within BBR_RS14485 at 1025.078 kb on + strand, within BBR_RS14485 at 1025.088 kb on + strand, within BBR_RS14485 at 1025.096 kb on - strand, within BBR_RS14485 at 1025.096 kb on - strand, within BBR_RS14485 at 1025.147 kb on - strand at 1025.159 kb on + strand at 1025.219 kb on + strand at 1025.222 kb on - strand at 1025.262 kb on + strand at 1025.270 kb on - strand at 1025.298 kb on - strand at 1025.326 kb on - strand, within BBR_RS14490 at 1025.326 kb on - strand, within BBR_RS14490 at 1025.342 kb on + strand, within BBR_RS14490 at 1025.371 kb on + strand, within BBR_RS14490 at 1025.371 kb on + strand, within BBR_RS14490 at 1025.406 kb on + strand, within BBR_RS14490 at 1025.409 kb on - strand, within BBR_RS14490 at 1025.414 kb on - strand, within BBR_RS14490 at 1025.517 kb on + strand, within BBR_RS14490 at 1025.525 kb on - strand, within BBR_RS14490 at 1025.667 kb on - strand, within BBR_RS14490 at 1025.681 kb on - strand, within BBR_RS14490 at 1025.683 kb on - strand, within BBR_RS14490 at 1025.698 kb on - strand, within BBR_RS14490 at 1025.726 kb on + strand, within BBR_RS14490 at 1025.739 kb on - strand, within BBR_RS14490 at 1025.777 kb on + strand, within BBR_RS14490 at 1025.785 kb on - strand, within BBR_RS14490 at 1025.787 kb on + strand, within BBR_RS14490 at 1025.787 kb on + strand, within BBR_RS14490 at 1025.851 kb on + strand, within BBR_RS14490 at 1025.883 kb on + strand, within BBR_RS14490 at 1025.915 kb on - strand, within BBR_RS14490 at 1025.944 kb on - strand, within BBR_RS14490 at 1025.949 kb on - strand, within BBR_RS14490 at 1025.952 kb on - strand, within BBR_RS14490 at 1025.960 kb on - strand, within BBR_RS14490 at 1025.960 kb on - strand, within BBR_RS14490 at 1026.021 kb on - strand, within BBR_RS14490 at 1026.031 kb on - strand, within BBR_RS14490 at 1026.035 kb on - strand, within BBR_RS14490 at 1026.037 kb on - strand, within BBR_RS14490 at 1026.056 kb on - strand, within BBR_RS14490 at 1026.133 kb on + strand, within BBR_RS14490 at 1026.133 kb on + strand, within BBR_RS14490 at 1026.146 kb on - strand, within BBR_RS14490 at 1026.155 kb on + strand, within BBR_RS14490 at 1026.212 kb on - strand, within BBR_RS14490 at 1026.261 kb on - strand, within BBR_RS14490 at 1026.265 kb on + strand, within BBR_RS14490 at 1026.279 kb on - strand, within BBR_RS14490 at 1026.517 kb on + strand at 1026.639 kb on + strand at 1026.760 kb on + strand, within BBR_RS14495 at 1027.204 kb on + strand, within BBR_RS14495 at 1027.217 kb on + strand, within BBR_RS14495 at 1027.257 kb on + strand, within BBR_RS14495 at 1027.304 kb on + strand, within BBR_RS14495 at 1027.425 kb on + strand, within BBR_RS14495 at 1027.461 kb on + strand, within BBR_RS14495
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Streptomycin 0.3 mM remove 1,024,178 - BBR_RS14480 0.79 -1.6 1,024,250 + BBR_RS14480 0.88 -0.2 1,024,258 - BBR_RS14480 0.88 -1.9 1,024,299 - +0.3 1,024,349 - -0.4 1,024,361 - +0.9 1,024,383 - -2.0 1,024,391 - -1.0 1,024,437 - +1.5 1,024,444 + +1.1 1,024,494 - +1.0 1,024,569 - +1.9 1,024,569 - +1.5 1,024,595 + -0.7 1,024,605 - BBR_RS14485 0.10 +0.3 1,024,617 + BBR_RS14485 0.12 +2.2 1,024,617 + BBR_RS14485 0.12 +1.3 1,024,617 + BBR_RS14485 0.12 +0.1 1,024,692 + BBR_RS14485 0.24 +1.1 1,024,699 - BBR_RS14485 0.25 +1.1 1,024,726 + BBR_RS14485 0.29 +1.2 1,024,838 + BBR_RS14485 0.47 +1.0 1,024,905 + BBR_RS14485 0.58 +2.8 1,024,994 - BBR_RS14485 0.72 +1.0 1,024,999 - BBR_RS14485 0.72 +0.8 1,025,071 - BBR_RS14485 0.84 +2.6 1,025,071 - BBR_RS14485 0.84 +0.3 1,025,078 + BBR_RS14485 0.85 +2.4 1,025,088 + BBR_RS14485 0.86 +2.6 1,025,096 - BBR_RS14485 0.88 -1.9 1,025,096 - BBR_RS14485 0.88 +3.7 1,025,147 - -0.5 1,025,159 + +1.3 1,025,219 + -1.4 1,025,222 - +0.6 1,025,262 + +1.0 1,025,270 - +0.4 1,025,298 - -0.9 1,025,326 - BBR_RS14490 0.12 -0.6 1,025,326 - BBR_RS14490 0.12 -2.0 1,025,342 + BBR_RS14490 0.13 -1.6 1,025,371 + BBR_RS14490 0.15 -0.3 1,025,371 + BBR_RS14490 0.15 +0.2 1,025,406 + BBR_RS14490 0.18 +0.4 1,025,409 - BBR_RS14490 0.18 -0.4 1,025,414 - BBR_RS14490 0.19 -0.3 1,025,517 + BBR_RS14490 0.27 +1.3 1,025,525 - BBR_RS14490 0.27 -0.1 1,025,667 - BBR_RS14490 0.38 -1.5 1,025,681 - BBR_RS14490 0.39 -0.0 1,025,683 - BBR_RS14490 0.39 +0.6 1,025,698 - BBR_RS14490 0.41 -0.5 1,025,726 + BBR_RS14490 0.43 +0.6 1,025,739 - BBR_RS14490 0.44 -1.8 1,025,777 + BBR_RS14490 0.47 -1.2 1,025,785 - BBR_RS14490 0.47 -0.7 1,025,787 + BBR_RS14490 0.47 -1.0 1,025,787 + BBR_RS14490 0.47 -1.7 1,025,851 + BBR_RS14490 0.52 +2.4 1,025,883 + BBR_RS14490 0.55 +0.1 1,025,915 - BBR_RS14490 0.57 +0.1 1,025,944 - BBR_RS14490 0.59 -0.5 1,025,949 - BBR_RS14490 0.60 +0.6 1,025,952 - BBR_RS14490 0.60 -2.2 1,025,960 - BBR_RS14490 0.61 +0.2 1,025,960 - BBR_RS14490 0.61 -1.0 1,026,021 - BBR_RS14490 0.65 +0.7 1,026,031 - BBR_RS14490 0.66 +0.7 1,026,035 - BBR_RS14490 0.66 +0.3 1,026,037 - BBR_RS14490 0.67 +1.5 1,026,056 - BBR_RS14490 0.68 -0.6 1,026,133 + BBR_RS14490 0.74 -2.3 1,026,133 + BBR_RS14490 0.74 -0.9 1,026,146 - BBR_RS14490 0.75 +0.5 1,026,155 + BBR_RS14490 0.76 -1.8 1,026,212 - BBR_RS14490 0.80 -0.4 1,026,261 - BBR_RS14490 0.84 -2.0 1,026,265 + BBR_RS14490 0.84 -1.9 1,026,279 - BBR_RS14490 0.85 +0.7 1,026,517 + +1.6 1,026,639 + -0.5 1,026,760 + BBR_RS14495 0.17 -1.2 1,027,204 + BBR_RS14495 0.49 +1.0 1,027,217 + BBR_RS14495 0.50 +0.9 1,027,257 + BBR_RS14495 0.53 +0.9 1,027,304 + BBR_RS14495 0.57 -0.5 1,027,425 + BBR_RS14495 0.65 +0.7 1,027,461 + BBR_RS14495 0.68 +0.6
Or see this region's nucleotide sequence