Experiment: Recovery broth with Streptomycin 0.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS14205 and BBR_RS14210 overlap by 4 nucleotides BBR_RS14210 and BBR_RS14215 are separated by 21 nucleotides BBR_RS14215 and BBR_RS14220 are separated by 151 nucleotides
BBR_RS14205: BBR_RS14205 - hypothetical protein, at 960,443 to 963,535
_RS14205
BBR_RS14210: BBR_RS14210 - hypothetical protein, at 963,532 to 965,073
_RS14210
BBR_RS14215: BBR_RS14215 - peptidase, at 965,095 to 965,457
_RS14215
BBR_RS14220: BBR_RS14220 - phosphomannomutase, at 965,609 to 966,412
_RS14220
Position (kb) 963
964
965
966 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 962.742 kb on - strand, within BBR_RS14205 at 962.794 kb on + strand, within BBR_RS14205 at 962.802 kb on - strand, within BBR_RS14205 at 962.827 kb on + strand, within BBR_RS14205 at 962.830 kb on + strand, within BBR_RS14205 at 962.836 kb on + strand, within BBR_RS14205 at 962.946 kb on + strand, within BBR_RS14205 at 963.245 kb on + strand at 963.275 kb on - strand at 963.383 kb on + strand at 963.391 kb on + strand at 963.391 kb on + strand at 963.392 kb on + strand at 963.399 kb on - strand at 963.414 kb on + strand at 963.422 kb on - strand at 963.456 kb on - strand at 963.483 kb on - strand at 963.495 kb on + strand at 963.516 kb on + strand at 963.555 kb on + strand at 963.709 kb on - strand, within BBR_RS14210 at 963.993 kb on - strand, within BBR_RS14210 at 964.075 kb on + strand, within BBR_RS14210 at 964.448 kb on - strand, within BBR_RS14210 at 964.526 kb on - strand, within BBR_RS14210 at 965.078 kb on - strand at 965.129 kb on - strand at 965.220 kb on - strand, within BBR_RS14215 at 965.232 kb on + strand, within BBR_RS14215 at 965.235 kb on - strand, within BBR_RS14215 at 965.240 kb on - strand, within BBR_RS14215 at 965.358 kb on + strand, within BBR_RS14215 at 965.358 kb on + strand, within BBR_RS14215 at 965.361 kb on - strand, within BBR_RS14215 at 965.454 kb on + strand at 965.471 kb on + strand at 965.472 kb on - strand at 965.479 kb on - strand at 965.503 kb on + strand at 965.570 kb on - strand at 965.589 kb on - strand at 965.620 kb on + strand at 965.673 kb on + strand at 965.678 kb on + strand at 965.681 kb on - strand at 965.700 kb on + strand, within BBR_RS14220 at 965.700 kb on + strand, within BBR_RS14220 at 965.703 kb on - strand, within BBR_RS14220 at 965.708 kb on - strand, within BBR_RS14220 at 965.710 kb on + strand, within BBR_RS14220 at 965.786 kb on + strand, within BBR_RS14220 at 965.810 kb on - strand, within BBR_RS14220 at 965.834 kb on - strand, within BBR_RS14220 at 965.862 kb on - strand, within BBR_RS14220 at 965.865 kb on + strand, within BBR_RS14220 at 965.883 kb on + strand, within BBR_RS14220 at 965.891 kb on + strand, within BBR_RS14220 at 965.897 kb on - strand, within BBR_RS14220 at 965.919 kb on - strand, within BBR_RS14220 at 965.961 kb on + strand, within BBR_RS14220 at 965.984 kb on - strand, within BBR_RS14220
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Streptomycin 0.3 mM remove 962,742 - BBR_RS14205 0.74 -0.3 962,794 + BBR_RS14205 0.76 +0.0 962,802 - BBR_RS14205 0.76 +1.1 962,827 + BBR_RS14205 0.77 -0.4 962,830 + BBR_RS14205 0.77 -3.0 962,836 + BBR_RS14205 0.77 -0.3 962,946 + BBR_RS14205 0.81 -0.6 963,245 + -0.0 963,275 - +0.1 963,383 + +0.8 963,391 + -1.4 963,391 + -0.4 963,392 + +0.3 963,399 - +0.5 963,414 + -0.3 963,422 - -0.5 963,456 - +1.4 963,483 - -0.9 963,495 + +0.3 963,516 + +0.4 963,555 + -1.4 963,709 - BBR_RS14210 0.11 -0.9 963,993 - BBR_RS14210 0.30 -0.9 964,075 + BBR_RS14210 0.35 -0.5 964,448 - BBR_RS14210 0.59 -0.8 964,526 - BBR_RS14210 0.64 -0.4 965,078 - +2.0 965,129 - -1.2 965,220 - BBR_RS14215 0.34 -0.1 965,232 + BBR_RS14215 0.38 -1.1 965,235 - BBR_RS14215 0.39 -0.2 965,240 - BBR_RS14215 0.40 +0.1 965,358 + BBR_RS14215 0.72 -0.2 965,358 + BBR_RS14215 0.72 +0.1 965,361 - BBR_RS14215 0.73 -1.2 965,454 + -1.9 965,471 + -2.4 965,472 - -1.6 965,479 - -0.4 965,503 + -0.8 965,570 - -0.1 965,589 - +0.7 965,620 + -0.7 965,673 + +3.0 965,678 + +2.0 965,681 - -0.8 965,700 + BBR_RS14220 0.11 +0.5 965,700 + BBR_RS14220 0.11 +1.2 965,703 - BBR_RS14220 0.12 -1.2 965,708 - BBR_RS14220 0.12 +2.4 965,710 + BBR_RS14220 0.13 +1.7 965,786 + BBR_RS14220 0.22 +1.6 965,810 - BBR_RS14220 0.25 +0.7 965,834 - BBR_RS14220 0.28 +0.0 965,862 - BBR_RS14220 0.31 +0.2 965,865 + BBR_RS14220 0.32 +0.0 965,883 + BBR_RS14220 0.34 -0.7 965,891 + BBR_RS14220 0.35 +1.2 965,897 - BBR_RS14220 0.36 +0.3 965,919 - BBR_RS14220 0.39 +2.3 965,961 + BBR_RS14220 0.44 +2.1 965,984 - BBR_RS14220 0.47 +0.9
Or see this region's nucleotide sequence