Experiment: Recovery broth with Streptomycin 0.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS13505 and BBR_RS13510 are separated by 17 nucleotides BBR_RS13510 and BBR_RS13515 are separated by 114 nucleotides BBR_RS13515 and BBR_RS13520 are separated by 84 nucleotides BBR_RS13520 and BBR_RS13525 are separated by 80 nucleotides
BBR_RS13505: BBR_RS13505 - DUF4391 domain-containing protein, at 803,720 to 804,451
_RS13505
BBR_RS13510: BBR_RS13510 - LysR family transcriptional regulator, at 804,469 to 805,284
_RS13510
BBR_RS13515: BBR_RS13515 - putative sulfate exporter family transporter, at 805,399 to 806,463
_RS13515
BBR_RS13520: BBR_RS13520 - polysaccharide synthesis protein GtrA, at 806,548 to 807,035
_RS13520
BBR_RS13525: BBR_RS13525 - CrcB family protein, at 807,116 to 807,481
_RS13525
Position (kb) 805
806
807 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5
6 at 804.439 kb on - strand at 804.446 kb on + strand at 804.446 kb on + strand at 804.446 kb on + strand at 804.446 kb on + strand at 804.446 kb on + strand at 804.446 kb on + strand at 804.446 kb on + strand at 804.454 kb on - strand at 804.454 kb on - strand at 804.459 kb on + strand at 804.467 kb on - strand at 804.488 kb on - strand at 804.499 kb on + strand at 804.506 kb on + strand at 804.509 kb on - strand at 804.516 kb on + strand at 804.516 kb on + strand at 804.596 kb on + strand, within BBR_RS13510 at 804.599 kb on - strand, within BBR_RS13510 at 804.630 kb on + strand, within BBR_RS13510 at 804.653 kb on - strand, within BBR_RS13510 at 804.783 kb on - strand, within BBR_RS13510 at 804.876 kb on + strand, within BBR_RS13510 at 804.876 kb on + strand, within BBR_RS13510 at 804.884 kb on - strand, within BBR_RS13510 at 804.889 kb on + strand, within BBR_RS13510 at 804.935 kb on + strand, within BBR_RS13510 at 804.935 kb on + strand, within BBR_RS13510 at 804.935 kb on + strand, within BBR_RS13510 at 804.943 kb on - strand, within BBR_RS13510 at 804.943 kb on - strand, within BBR_RS13510 at 804.943 kb on - strand, within BBR_RS13510 at 804.954 kb on - strand, within BBR_RS13510 at 804.956 kb on + strand, within BBR_RS13510 at 804.991 kb on - strand, within BBR_RS13510 at 805.007 kb on + strand, within BBR_RS13510 at 805.007 kb on + strand, within BBR_RS13510 at 805.028 kb on - strand, within BBR_RS13510 at 805.056 kb on - strand, within BBR_RS13510 at 805.061 kb on - strand, within BBR_RS13510 at 805.078 kb on - strand, within BBR_RS13510 at 805.080 kb on + strand, within BBR_RS13510 at 805.126 kb on - strand, within BBR_RS13510 at 805.126 kb on - strand, within BBR_RS13510 at 805.152 kb on + strand, within BBR_RS13510 at 805.152 kb on + strand, within BBR_RS13510 at 805.154 kb on + strand, within BBR_RS13510 at 805.162 kb on - strand, within BBR_RS13510 at 805.228 kb on - strand at 805.309 kb on - strand at 805.441 kb on + strand at 805.603 kb on - strand, within BBR_RS13515 at 805.613 kb on + strand, within BBR_RS13515 at 805.667 kb on + strand, within BBR_RS13515 at 805.700 kb on - strand, within BBR_RS13515 at 805.702 kb on + strand, within BBR_RS13515 at 805.716 kb on + strand, within BBR_RS13515 at 805.716 kb on + strand, within BBR_RS13515 at 805.770 kb on + strand, within BBR_RS13515 at 805.775 kb on + strand, within BBR_RS13515 at 805.778 kb on - strand, within BBR_RS13515 at 805.791 kb on + strand, within BBR_RS13515 at 805.804 kb on - strand, within BBR_RS13515 at 805.824 kb on - strand, within BBR_RS13515 at 805.824 kb on - strand, within BBR_RS13515 at 805.824 kb on - strand, within BBR_RS13515 at 805.864 kb on + strand, within BBR_RS13515 at 805.872 kb on - strand, within BBR_RS13515 at 805.931 kb on - strand, within BBR_RS13515 at 805.956 kb on - strand, within BBR_RS13515 at 805.969 kb on + strand, within BBR_RS13515 at 805.969 kb on + strand, within BBR_RS13515 at 806.016 kb on + strand, within BBR_RS13515 at 806.027 kb on - strand, within BBR_RS13515 at 806.027 kb on - strand, within BBR_RS13515 at 806.078 kb on + strand, within BBR_RS13515 at 806.092 kb on - strand, within BBR_RS13515 at 806.120 kb on - strand, within BBR_RS13515 at 806.177 kb on + strand, within BBR_RS13515 at 806.182 kb on + strand, within BBR_RS13515 at 806.187 kb on + strand, within BBR_RS13515 at 806.242 kb on - strand, within BBR_RS13515 at 806.253 kb on + strand, within BBR_RS13515 at 806.309 kb on + strand, within BBR_RS13515 at 806.324 kb on + strand, within BBR_RS13515 at 806.336 kb on - strand, within BBR_RS13515 at 806.336 kb on - strand, within BBR_RS13515 at 806.336 kb on - strand, within BBR_RS13515 at 806.336 kb on - strand, within BBR_RS13515 at 806.336 kb on - strand, within BBR_RS13515 at 806.358 kb on + strand at 806.358 kb on + strand at 806.387 kb on - strand at 806.403 kb on - strand at 806.449 kb on + strand at 806.449 kb on + strand at 806.449 kb on - strand at 806.465 kb on - strand at 806.465 kb on - strand at 806.495 kb on - strand at 806.497 kb on + strand at 806.530 kb on + strand at 806.538 kb on - strand at 806.539 kb on - strand at 806.564 kb on + strand at 806.606 kb on + strand, within BBR_RS13520 at 806.748 kb on + strand, within BBR_RS13520 at 806.767 kb on - strand, within BBR_RS13520 at 806.767 kb on - strand, within BBR_RS13520 at 806.797 kb on + strand, within BBR_RS13520 at 806.821 kb on + strand, within BBR_RS13520 at 806.858 kb on + strand, within BBR_RS13520 at 806.866 kb on + strand, within BBR_RS13520 at 806.874 kb on - strand, within BBR_RS13520 at 806.874 kb on - strand, within BBR_RS13520 at 806.875 kb on + strand, within BBR_RS13520 at 806.876 kb on - strand, within BBR_RS13520 at 806.876 kb on - strand, within BBR_RS13520 at 806.878 kb on - strand, within BBR_RS13520 at 806.892 kb on + strand, within BBR_RS13520 at 806.902 kb on - strand, within BBR_RS13520 at 807.004 kb on - strand at 807.006 kb on - strand at 807.032 kb on - strand at 807.045 kb on - strand at 807.114 kb on + strand at 807.164 kb on - strand, within BBR_RS13525 at 807.164 kb on - strand, within BBR_RS13525 at 807.169 kb on + strand, within BBR_RS13525 at 807.186 kb on - strand, within BBR_RS13525 at 807.189 kb on + strand, within BBR_RS13525 at 807.201 kb on + strand, within BBR_RS13525 at 807.234 kb on + strand, within BBR_RS13525 at 807.253 kb on - strand, within BBR_RS13525 at 807.269 kb on + strand, within BBR_RS13525 at 807.409 kb on + strand, within BBR_RS13525
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Streptomycin 0.3 mM remove 804,439 - +0.6 804,446 + -1.4 804,446 + +1.6 804,446 + +0.8 804,446 + +0.4 804,446 + -1.9 804,446 + +0.2 804,446 + -0.5 804,454 - +0.4 804,454 - +0.6 804,459 + -0.9 804,467 - +0.1 804,488 - +2.7 804,499 + -1.2 804,506 + +0.0 804,509 - -2.7 804,516 + -0.4 804,516 + +0.3 804,596 + BBR_RS13510 0.16 +0.1 804,599 - BBR_RS13510 0.16 +2.0 804,630 + BBR_RS13510 0.20 +0.4 804,653 - BBR_RS13510 0.23 +0.6 804,783 - BBR_RS13510 0.38 -1.5 804,876 + BBR_RS13510 0.50 -0.0 804,876 + BBR_RS13510 0.50 +0.2 804,884 - BBR_RS13510 0.51 +0.1 804,889 + BBR_RS13510 0.51 -2.0 804,935 + BBR_RS13510 0.57 -0.8 804,935 + BBR_RS13510 0.57 +1.4 804,935 + BBR_RS13510 0.57 -1.2 804,943 - BBR_RS13510 0.58 -0.6 804,943 - BBR_RS13510 0.58 +1.2 804,943 - BBR_RS13510 0.58 -0.1 804,954 - BBR_RS13510 0.59 -0.2 804,956 + BBR_RS13510 0.60 -0.4 804,991 - BBR_RS13510 0.64 -0.2 805,007 + BBR_RS13510 0.66 +1.7 805,007 + BBR_RS13510 0.66 -1.4 805,028 - BBR_RS13510 0.69 +1.5 805,056 - BBR_RS13510 0.72 +0.6 805,061 - BBR_RS13510 0.73 +0.1 805,078 - BBR_RS13510 0.75 -0.7 805,080 + BBR_RS13510 0.75 -1.9 805,126 - BBR_RS13510 0.81 +1.3 805,126 - BBR_RS13510 0.81 +0.7 805,152 + BBR_RS13510 0.84 +1.5 805,152 + BBR_RS13510 0.84 +0.8 805,154 + BBR_RS13510 0.84 -1.0 805,162 - BBR_RS13510 0.85 +1.1 805,228 - +1.0 805,309 - -1.4 805,441 + +1.6 805,603 - BBR_RS13515 0.19 +6.0 805,613 + BBR_RS13515 0.20 +3.9 805,667 + BBR_RS13515 0.25 +1.5 805,700 - BBR_RS13515 0.28 +3.5 805,702 + BBR_RS13515 0.28 +3.1 805,716 + BBR_RS13515 0.30 +2.4 805,716 + BBR_RS13515 0.30 +3.3 805,770 + BBR_RS13515 0.35 +2.6 805,775 + BBR_RS13515 0.35 +1.4 805,778 - BBR_RS13515 0.36 +4.8 805,791 + BBR_RS13515 0.37 +2.4 805,804 - BBR_RS13515 0.38 +3.1 805,824 - BBR_RS13515 0.40 +4.8 805,824 - BBR_RS13515 0.40 +4.7 805,824 - BBR_RS13515 0.40 +0.5 805,864 + BBR_RS13515 0.44 +0.3 805,872 - BBR_RS13515 0.44 +3.0 805,931 - BBR_RS13515 0.50 +2.6 805,956 - BBR_RS13515 0.52 +2.8 805,969 + BBR_RS13515 0.54 +2.5 805,969 + BBR_RS13515 0.54 +2.4 806,016 + BBR_RS13515 0.58 -0.1 806,027 - BBR_RS13515 0.59 -0.2 806,027 - BBR_RS13515 0.59 -0.4 806,078 + BBR_RS13515 0.64 +2.0 806,092 - BBR_RS13515 0.65 +1.8 806,120 - BBR_RS13515 0.68 +1.2 806,177 + BBR_RS13515 0.73 +4.0 806,182 + BBR_RS13515 0.74 +0.5 806,187 + BBR_RS13515 0.74 +4.0 806,242 - BBR_RS13515 0.79 +2.2 806,253 + BBR_RS13515 0.80 +0.9 806,309 + BBR_RS13515 0.85 -0.4 806,324 + BBR_RS13515 0.87 +0.9 806,336 - BBR_RS13515 0.88 +3.3 806,336 - BBR_RS13515 0.88 +2.8 806,336 - BBR_RS13515 0.88 +2.6 806,336 - BBR_RS13515 0.88 +2.4 806,336 - BBR_RS13515 0.88 +5.1 806,358 + +3.2 806,358 + +0.9 806,387 - +2.7 806,403 - +3.7 806,449 + -1.9 806,449 + -0.8 806,449 - +0.1 806,465 - +2.5 806,465 - +0.1 806,495 - +2.3 806,497 + +1.9 806,530 + +2.4 806,538 - +1.4 806,539 - +0.3 806,564 + -0.4 806,606 + BBR_RS13520 0.12 +1.8 806,748 + BBR_RS13520 0.41 -0.2 806,767 - BBR_RS13520 0.45 +2.2 806,767 - BBR_RS13520 0.45 -1.7 806,797 + BBR_RS13520 0.51 +0.6 806,821 + BBR_RS13520 0.56 +0.8 806,858 + BBR_RS13520 0.64 -0.3 806,866 + BBR_RS13520 0.65 -0.5 806,874 - BBR_RS13520 0.67 +2.5 806,874 - BBR_RS13520 0.67 +2.7 806,875 + BBR_RS13520 0.67 -1.2 806,876 - BBR_RS13520 0.67 -1.4 806,876 - BBR_RS13520 0.67 -0.0 806,878 - BBR_RS13520 0.68 +1.2 806,892 + BBR_RS13520 0.70 -0.4 806,902 - BBR_RS13520 0.73 -2.1 807,004 - +0.8 807,006 - +1.6 807,032 - +0.3 807,045 - +0.8 807,114 + -0.5 807,164 - BBR_RS13525 0.13 -0.3 807,164 - BBR_RS13525 0.13 +1.4 807,169 + BBR_RS13525 0.14 -0.0 807,186 - BBR_RS13525 0.19 +1.0 807,189 + BBR_RS13525 0.20 -1.2 807,201 + BBR_RS13525 0.23 +1.8 807,234 + BBR_RS13525 0.32 +1.8 807,253 - BBR_RS13525 0.37 -2.3 807,269 + BBR_RS13525 0.42 -0.0 807,409 + BBR_RS13525 0.80 -0.4
Or see this region's nucleotide sequence