Experiment: Recovery broth with Streptomycin 0.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS13000 and BBR_RS13005 are separated by 41 nucleotides BBR_RS13005 and BBR_RS20740 are separated by 227 nucleotides BBR_RS20740 and BBR_RS13010 are separated by 16 nucleotides
BBR_RS13000: BBR_RS13000 - RNase J family beta-CASP ribonuclease, at 686,538 to 688,394
_RS13000
BBR_RS13005: BBR_RS13005 - aminopeptidase N, at 688,436 to 691,045
_RS13005
BBR_RS20740: BBR_RS20740 - hypothetical protein, at 691,273 to 691,515
_RS20740
BBR_RS13010: BBR_RS13010 - phosphoglucosamine mutase, at 691,532 to 692,917
_RS13010
Position (kb) 688
689
690
691
692 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 688.003 kb on + strand, within BBR_RS13000 at 688.362 kb on + strand at 688.388 kb on + strand at 688.388 kb on + strand at 688.403 kb on + strand at 688.422 kb on - strand at 688.485 kb on + strand at 688.504 kb on + strand at 688.523 kb on - strand at 688.608 kb on - strand at 688.618 kb on + strand at 688.619 kb on - strand at 688.653 kb on - strand at 688.680 kb on + strand at 688.680 kb on + strand at 688.680 kb on + strand at 688.694 kb on + strand at 688.727 kb on + strand, within BBR_RS13005 at 688.741 kb on + strand, within BBR_RS13005 at 688.749 kb on - strand, within BBR_RS13005 at 688.757 kb on + strand, within BBR_RS13005 at 688.757 kb on + strand, within BBR_RS13005 at 688.757 kb on + strand, within BBR_RS13005 at 688.834 kb on + strand, within BBR_RS13005 at 688.834 kb on + strand, within BBR_RS13005 at 688.834 kb on + strand, within BBR_RS13005 at 688.854 kb on + strand, within BBR_RS13005 at 688.854 kb on + strand, within BBR_RS13005 at 688.859 kb on + strand, within BBR_RS13005 at 688.897 kb on + strand, within BBR_RS13005 at 688.958 kb on + strand, within BBR_RS13005 at 688.966 kb on - strand, within BBR_RS13005 at 688.966 kb on - strand, within BBR_RS13005 at 688.973 kb on - strand, within BBR_RS13005 at 688.987 kb on + strand, within BBR_RS13005 at 688.992 kb on + strand, within BBR_RS13005 at 689.034 kb on + strand, within BBR_RS13005 at 689.034 kb on + strand, within BBR_RS13005 at 689.034 kb on + strand, within BBR_RS13005 at 689.034 kb on + strand, within BBR_RS13005 at 689.034 kb on + strand, within BBR_RS13005 at 689.055 kb on + strand, within BBR_RS13005 at 689.063 kb on - strand, within BBR_RS13005 at 689.096 kb on - strand, within BBR_RS13005 at 689.103 kb on + strand, within BBR_RS13005 at 689.103 kb on + strand, within BBR_RS13005 at 689.110 kb on - strand, within BBR_RS13005 at 689.137 kb on + strand, within BBR_RS13005 at 689.137 kb on + strand, within BBR_RS13005 at 689.215 kb on + strand, within BBR_RS13005 at 689.215 kb on + strand, within BBR_RS13005 at 689.215 kb on + strand, within BBR_RS13005 at 689.239 kb on - strand, within BBR_RS13005 at 689.252 kb on - strand, within BBR_RS13005 at 689.254 kb on + strand, within BBR_RS13005 at 689.272 kb on - strand, within BBR_RS13005 at 689.283 kb on + strand, within BBR_RS13005 at 689.285 kb on + strand, within BBR_RS13005 at 689.303 kb on + strand, within BBR_RS13005 at 689.316 kb on + strand, within BBR_RS13005 at 689.339 kb on - strand, within BBR_RS13005 at 689.375 kb on + strand, within BBR_RS13005 at 689.375 kb on + strand, within BBR_RS13005 at 689.410 kb on + strand, within BBR_RS13005 at 689.437 kb on + strand, within BBR_RS13005 at 689.442 kb on + strand, within BBR_RS13005 at 689.462 kb on + strand, within BBR_RS13005 at 689.464 kb on + strand, within BBR_RS13005 at 689.486 kb on + strand, within BBR_RS13005 at 689.504 kb on - strand, within BBR_RS13005 at 689.539 kb on + strand, within BBR_RS13005 at 689.541 kb on + strand, within BBR_RS13005 at 689.566 kb on + strand, within BBR_RS13005 at 689.571 kb on - strand, within BBR_RS13005 at 689.574 kb on - strand, within BBR_RS13005 at 689.578 kb on + strand, within BBR_RS13005 at 689.649 kb on - strand, within BBR_RS13005 at 689.674 kb on + strand, within BBR_RS13005 at 689.674 kb on + strand, within BBR_RS13005 at 689.698 kb on + strand, within BBR_RS13005 at 689.716 kb on - strand, within BBR_RS13005 at 689.732 kb on + strand, within BBR_RS13005 at 689.734 kb on - strand, within BBR_RS13005 at 689.742 kb on + strand, within BBR_RS13005 at 689.742 kb on + strand, within BBR_RS13005 at 689.802 kb on - strand, within BBR_RS13005 at 689.817 kb on + strand, within BBR_RS13005 at 689.817 kb on + strand, within BBR_RS13005 at 689.877 kb on + strand, within BBR_RS13005 at 689.887 kb on + strand, within BBR_RS13005 at 689.887 kb on + strand, within BBR_RS13005 at 689.895 kb on - strand, within BBR_RS13005 at 689.895 kb on - strand, within BBR_RS13005 at 689.897 kb on + strand, within BBR_RS13005 at 689.939 kb on + strand, within BBR_RS13005 at 689.992 kb on + strand, within BBR_RS13005 at 689.996 kb on + strand, within BBR_RS13005 at 690.058 kb on + strand, within BBR_RS13005 at 690.065 kb on + strand, within BBR_RS13005 at 690.076 kb on + strand, within BBR_RS13005 at 690.089 kb on + strand, within BBR_RS13005 at 690.089 kb on + strand, within BBR_RS13005 at 690.089 kb on + strand, within BBR_RS13005 at 690.089 kb on + strand, within BBR_RS13005 at 690.167 kb on + strand, within BBR_RS13005 at 690.167 kb on + strand, within BBR_RS13005 at 690.172 kb on + strand, within BBR_RS13005 at 690.175 kb on - strand, within BBR_RS13005 at 690.230 kb on + strand, within BBR_RS13005 at 690.252 kb on - strand, within BBR_RS13005 at 690.294 kb on + strand, within BBR_RS13005 at 690.294 kb on + strand, within BBR_RS13005 at 690.294 kb on + strand, within BBR_RS13005 at 690.294 kb on + strand, within BBR_RS13005 at 690.299 kb on + strand, within BBR_RS13005 at 690.299 kb on + strand, within BBR_RS13005 at 690.299 kb on + strand, within BBR_RS13005 at 690.302 kb on - strand, within BBR_RS13005 at 690.307 kb on - strand, within BBR_RS13005 at 690.323 kb on + strand, within BBR_RS13005 at 690.323 kb on + strand, within BBR_RS13005 at 690.328 kb on + strand, within BBR_RS13005 at 690.328 kb on + strand, within BBR_RS13005 at 690.340 kb on - strand, within BBR_RS13005 at 690.367 kb on - strand, within BBR_RS13005 at 690.389 kb on + strand, within BBR_RS13005 at 690.414 kb on + strand, within BBR_RS13005 at 690.427 kb on + strand, within BBR_RS13005 at 690.427 kb on + strand, within BBR_RS13005 at 690.464 kb on - strand, within BBR_RS13005 at 690.510 kb on + strand, within BBR_RS13005 at 690.513 kb on - strand, within BBR_RS13005 at 690.513 kb on - strand, within BBR_RS13005 at 690.541 kb on + strand, within BBR_RS13005 at 690.552 kb on - strand, within BBR_RS13005 at 690.576 kb on - strand, within BBR_RS13005 at 690.635 kb on + strand, within BBR_RS13005 at 690.641 kb on - strand, within BBR_RS13005 at 690.678 kb on + strand, within BBR_RS13005 at 690.683 kb on + strand, within BBR_RS13005 at 690.716 kb on + strand, within BBR_RS13005 at 690.726 kb on - strand, within BBR_RS13005 at 690.745 kb on + strand, within BBR_RS13005 at 690.745 kb on + strand, within BBR_RS13005 at 690.745 kb on + strand, within BBR_RS13005 at 690.746 kb on - strand, within BBR_RS13005 at 690.758 kb on + strand, within BBR_RS13005 at 690.833 kb on - strand at 690.833 kb on - strand at 690.863 kb on + strand at 690.863 kb on + strand at 690.871 kb on - strand at 690.892 kb on + strand at 690.944 kb on - strand at 691.011 kb on + strand at 691.011 kb on + strand at 691.011 kb on + strand at 691.011 kb on + strand at 691.011 kb on + strand at 691.011 kb on + strand at 691.011 kb on + strand at 691.011 kb on + strand at 691.011 kb on + strand at 691.011 kb on + strand at 691.011 kb on + strand at 691.011 kb on + strand at 691.011 kb on + strand at 691.011 kb on + strand at 691.011 kb on + strand at 691.011 kb on + strand at 691.011 kb on + strand at 691.011 kb on + strand at 691.011 kb on + strand at 691.013 kb on + strand at 691.019 kb on - strand at 691.019 kb on - strand at 691.019 kb on - strand at 691.019 kb on - strand at 691.057 kb on + strand at 691.060 kb on + strand at 691.060 kb on - strand at 691.099 kb on + strand at 691.122 kb on + strand at 691.124 kb on + strand at 691.132 kb on - strand at 691.151 kb on - strand at 691.166 kb on - strand at 691.166 kb on - strand at 691.185 kb on + strand at 691.217 kb on - strand at 691.222 kb on - strand at 691.261 kb on + strand at 691.271 kb on + strand at 691.276 kb on + strand at 691.280 kb on + strand at 691.296 kb on + strand at 691.321 kb on - strand, within BBR_RS20740 at 691.370 kb on + strand, within BBR_RS20740 at 691.370 kb on + strand, within BBR_RS20740 at 691.370 kb on + strand, within BBR_RS20740 at 691.378 kb on - strand, within BBR_RS20740 at 691.378 kb on - strand, within BBR_RS20740 at 691.378 kb on - strand, within BBR_RS20740 at 691.378 kb on - strand, within BBR_RS20740 at 691.378 kb on - strand, within BBR_RS20740 at 691.378 kb on - strand, within BBR_RS20740 at 691.387 kb on - strand, within BBR_RS20740 at 691.472 kb on + strand, within BBR_RS20740 at 691.480 kb on - strand, within BBR_RS20740 at 691.490 kb on + strand, within BBR_RS20740 at 691.494 kb on - strand at 691.517 kb on + strand at 691.525 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Streptomycin 0.3 mM remove 688,003 + BBR_RS13000 0.79 -0.5 688,362 + +0.0 688,388 + +0.8 688,388 + +0.8 688,403 + +0.6 688,422 - +0.9 688,485 + -1.3 688,504 + -0.8 688,523 - -0.9 688,608 - -1.0 688,618 + +1.1 688,619 - +1.5 688,653 - +0.9 688,680 + +0.8 688,680 + -0.0 688,680 + -0.2 688,694 + -0.6 688,727 + BBR_RS13005 0.11 -2.2 688,741 + BBR_RS13005 0.12 -0.6 688,749 - BBR_RS13005 0.12 -1.1 688,757 + BBR_RS13005 0.12 -0.9 688,757 + BBR_RS13005 0.12 -0.6 688,757 + BBR_RS13005 0.12 -0.5 688,834 + BBR_RS13005 0.15 -1.6 688,834 + BBR_RS13005 0.15 -0.3 688,834 + BBR_RS13005 0.15 -0.0 688,854 + BBR_RS13005 0.16 -0.4 688,854 + BBR_RS13005 0.16 -0.2 688,859 + BBR_RS13005 0.16 +1.0 688,897 + BBR_RS13005 0.18 -1.5 688,958 + BBR_RS13005 0.20 -0.4 688,966 - BBR_RS13005 0.20 -0.6 688,966 - BBR_RS13005 0.20 -1.3 688,973 - BBR_RS13005 0.21 +2.6 688,987 + BBR_RS13005 0.21 -0.5 688,992 + BBR_RS13005 0.21 +2.0 689,034 + BBR_RS13005 0.23 -2.1 689,034 + BBR_RS13005 0.23 -1.8 689,034 + BBR_RS13005 0.23 -0.6 689,034 + BBR_RS13005 0.23 +0.1 689,034 + BBR_RS13005 0.23 +0.3 689,055 + BBR_RS13005 0.24 -1.1 689,063 - BBR_RS13005 0.24 +0.3 689,096 - BBR_RS13005 0.25 -0.8 689,103 + BBR_RS13005 0.26 -0.1 689,103 + BBR_RS13005 0.26 -1.7 689,110 - BBR_RS13005 0.26 -0.5 689,137 + BBR_RS13005 0.27 -0.9 689,137 + BBR_RS13005 0.27 -2.0 689,215 + BBR_RS13005 0.30 +0.5 689,215 + BBR_RS13005 0.30 -2.1 689,215 + BBR_RS13005 0.30 -2.0 689,239 - BBR_RS13005 0.31 -0.8 689,252 - BBR_RS13005 0.31 +0.6 689,254 + BBR_RS13005 0.31 -0.9 689,272 - BBR_RS13005 0.32 -0.4 689,283 + BBR_RS13005 0.32 -3.3 689,285 + BBR_RS13005 0.33 -0.1 689,303 + BBR_RS13005 0.33 -0.6 689,316 + BBR_RS13005 0.34 -1.7 689,339 - BBR_RS13005 0.35 -0.2 689,375 + BBR_RS13005 0.36 -2.5 689,375 + BBR_RS13005 0.36 -1.8 689,410 + BBR_RS13005 0.37 +0.3 689,437 + BBR_RS13005 0.38 -1.3 689,442 + BBR_RS13005 0.39 +1.3 689,462 + BBR_RS13005 0.39 -2.2 689,464 + BBR_RS13005 0.39 -0.7 689,486 + BBR_RS13005 0.40 -0.2 689,504 - BBR_RS13005 0.41 -1.3 689,539 + BBR_RS13005 0.42 -1.5 689,541 + BBR_RS13005 0.42 -0.7 689,566 + BBR_RS13005 0.43 -0.4 689,571 - BBR_RS13005 0.43 -1.2 689,574 - BBR_RS13005 0.44 -1.2 689,578 + BBR_RS13005 0.44 -1.7 689,649 - BBR_RS13005 0.46 -2.3 689,674 + BBR_RS13005 0.47 +0.3 689,674 + BBR_RS13005 0.47 -1.6 689,698 + BBR_RS13005 0.48 -1.5 689,716 - BBR_RS13005 0.49 -0.2 689,732 + BBR_RS13005 0.50 -1.3 689,734 - BBR_RS13005 0.50 -0.3 689,742 + BBR_RS13005 0.50 -0.7 689,742 + BBR_RS13005 0.50 +0.1 689,802 - BBR_RS13005 0.52 -0.9 689,817 + BBR_RS13005 0.53 -0.7 689,817 + BBR_RS13005 0.53 -1.5 689,877 + BBR_RS13005 0.55 -1.6 689,887 + BBR_RS13005 0.56 -2.4 689,887 + BBR_RS13005 0.56 +0.1 689,895 - BBR_RS13005 0.56 +2.3 689,895 - BBR_RS13005 0.56 +2.0 689,897 + BBR_RS13005 0.56 +0.1 689,939 + BBR_RS13005 0.58 -1.4 689,992 + BBR_RS13005 0.60 -2.4 689,996 + BBR_RS13005 0.60 -0.8 690,058 + BBR_RS13005 0.62 -1.1 690,065 + BBR_RS13005 0.62 -0.1 690,076 + BBR_RS13005 0.63 -1.5 690,089 + BBR_RS13005 0.63 -1.6 690,089 + BBR_RS13005 0.63 -0.6 690,089 + BBR_RS13005 0.63 +0.2 690,089 + BBR_RS13005 0.63 -0.2 690,167 + BBR_RS13005 0.66 -0.4 690,167 + BBR_RS13005 0.66 +0.7 690,172 + BBR_RS13005 0.67 +1.9 690,175 - BBR_RS13005 0.67 -1.2 690,230 + BBR_RS13005 0.69 -1.3 690,252 - BBR_RS13005 0.70 -1.1 690,294 + BBR_RS13005 0.71 -0.8 690,294 + BBR_RS13005 0.71 +0.1 690,294 + BBR_RS13005 0.71 -2.4 690,294 + BBR_RS13005 0.71 -0.3 690,299 + BBR_RS13005 0.71 +1.4 690,299 + BBR_RS13005 0.71 -0.0 690,299 + BBR_RS13005 0.71 -0.4 690,302 - BBR_RS13005 0.71 -1.5 690,307 - BBR_RS13005 0.72 -0.8 690,323 + BBR_RS13005 0.72 -0.7 690,323 + BBR_RS13005 0.72 +1.5 690,328 + BBR_RS13005 0.72 -1.1 690,328 + BBR_RS13005 0.72 -0.7 690,340 - BBR_RS13005 0.73 -0.6 690,367 - BBR_RS13005 0.74 -0.4 690,389 + BBR_RS13005 0.75 -2.3 690,414 + BBR_RS13005 0.76 -0.3 690,427 + BBR_RS13005 0.76 -1.4 690,427 + BBR_RS13005 0.76 -0.4 690,464 - BBR_RS13005 0.78 -1.2 690,510 + BBR_RS13005 0.79 +0.2 690,513 - BBR_RS13005 0.80 -1.5 690,513 - BBR_RS13005 0.80 -1.9 690,541 + BBR_RS13005 0.81 -1.5 690,552 - BBR_RS13005 0.81 +0.9 690,576 - BBR_RS13005 0.82 -0.2 690,635 + BBR_RS13005 0.84 +1.8 690,641 - BBR_RS13005 0.84 +0.1 690,678 + BBR_RS13005 0.86 +0.2 690,683 + BBR_RS13005 0.86 +0.6 690,716 + BBR_RS13005 0.87 +0.2 690,726 - BBR_RS13005 0.88 +1.0 690,745 + BBR_RS13005 0.88 +0.1 690,745 + BBR_RS13005 0.88 -0.3 690,745 + BBR_RS13005 0.88 -1.2 690,746 - BBR_RS13005 0.89 +1.5 690,758 + BBR_RS13005 0.89 -0.2 690,833 - -0.5 690,833 - -1.0 690,863 + -2.0 690,863 + -2.8 690,871 - -0.0 690,892 + -0.9 690,944 - +0.0 691,011 + +2.3 691,011 + +0.5 691,011 + -2.5 691,011 + -0.7 691,011 + +1.2 691,011 + -2.2 691,011 + +0.1 691,011 + -0.2 691,011 + +1.0 691,011 + +0.3 691,011 + +1.7 691,011 + +0.9 691,011 + -0.0 691,011 + -0.7 691,011 + -2.5 691,011 + +0.8 691,011 + +1.0 691,011 + +0.1 691,011 + -0.0 691,013 + -0.8 691,019 - -1.5 691,019 - -1.9 691,019 - -1.5 691,019 - +1.2 691,057 + -0.8 691,060 + -0.0 691,060 - -0.7 691,099 + +1.0 691,122 + -0.9 691,124 + -0.1 691,132 - -0.9 691,151 - +1.1 691,166 - +0.5 691,166 - +0.7 691,185 + -0.0 691,217 - -0.1 691,222 - +0.1 691,261 + +0.2 691,271 + +0.9 691,276 + -0.9 691,280 + +0.2 691,296 + -0.3 691,321 - BBR_RS20740 0.20 +0.5 691,370 + BBR_RS20740 0.40 +1.4 691,370 + BBR_RS20740 0.40 -2.0 691,370 + BBR_RS20740 0.40 +0.1 691,378 - BBR_RS20740 0.43 -1.5 691,378 - BBR_RS20740 0.43 +0.5 691,378 - BBR_RS20740 0.43 -0.8 691,378 - BBR_RS20740 0.43 +1.0 691,378 - BBR_RS20740 0.43 +0.6 691,378 - BBR_RS20740 0.43 +0.7 691,387 - BBR_RS20740 0.47 +1.1 691,472 + BBR_RS20740 0.82 -2.8 691,480 - BBR_RS20740 0.85 +0.2 691,490 + BBR_RS20740 0.89 -0.3 691,494 - -0.0 691,517 + +0.1 691,525 - -1.1
Or see this region's nucleotide sequence