Experiment: Recovery broth with Streptomycin 0.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS12935 and BBR_RS12940 are separated by 400 nucleotides BBR_RS12940 and BBR_RS12945 are separated by 234 nucleotides
BBR_RS12935: BBR_RS12935 - A/G-specific adenine glycosylase, at 653,605 to 654,567
_RS12935
BBR_RS12940: BBR_RS12940 - hypothetical protein, at 654,968 to 655,549
_RS12940
BBR_RS12945: BBR_RS12945 - DNA-directed RNA polymerase subunit beta, at 655,784 to 659,347
_RS12945
Position (kb) 654
655
656 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 653.977 kb on + strand, within BBR_RS12935 at 653.977 kb on + strand, within BBR_RS12935 at 653.977 kb on + strand, within BBR_RS12935 at 653.977 kb on + strand, within BBR_RS12935 at 653.977 kb on + strand, within BBR_RS12935 at 653.977 kb on + strand, within BBR_RS12935 at 653.985 kb on - strand, within BBR_RS12935 at 653.985 kb on - strand, within BBR_RS12935 at 654.052 kb on - strand, within BBR_RS12935 at 654.052 kb on - strand, within BBR_RS12935 at 654.084 kb on - strand, within BBR_RS12935 at 654.110 kb on - strand, within BBR_RS12935 at 654.123 kb on + strand, within BBR_RS12935 at 654.124 kb on + strand, within BBR_RS12935 at 654.124 kb on - strand, within BBR_RS12935 at 654.147 kb on + strand, within BBR_RS12935 at 654.147 kb on + strand, within BBR_RS12935 at 654.161 kb on + strand, within BBR_RS12935 at 654.190 kb on + strand, within BBR_RS12935 at 654.195 kb on + strand, within BBR_RS12935 at 654.195 kb on - strand, within BBR_RS12935 at 654.226 kb on - strand, within BBR_RS12935 at 654.240 kb on - strand, within BBR_RS12935 at 654.245 kb on + strand, within BBR_RS12935 at 654.260 kb on - strand, within BBR_RS12935 at 654.260 kb on - strand, within BBR_RS12935 at 654.262 kb on + strand, within BBR_RS12935 at 654.270 kb on - strand, within BBR_RS12935 at 654.312 kb on + strand, within BBR_RS12935 at 654.332 kb on + strand, within BBR_RS12935 at 654.332 kb on + strand, within BBR_RS12935 at 654.369 kb on + strand, within BBR_RS12935 at 654.377 kb on + strand, within BBR_RS12935 at 654.382 kb on + strand, within BBR_RS12935 at 654.387 kb on + strand, within BBR_RS12935 at 654.417 kb on - strand, within BBR_RS12935 at 654.480 kb on - strand at 654.535 kb on - strand at 654.539 kb on + strand at 654.555 kb on + strand at 654.577 kb on + strand at 654.577 kb on + strand at 654.577 kb on + strand at 654.577 kb on + strand at 654.577 kb on + strand at 654.577 kb on + strand at 654.585 kb on - strand at 654.585 kb on - strand at 654.585 kb on - strand at 654.585 kb on - strand at 654.587 kb on - strand at 654.587 kb on - strand at 654.587 kb on - strand at 654.611 kb on - strand at 654.611 kb on - strand at 654.620 kb on - strand at 654.620 kb on - strand at 654.626 kb on - strand at 654.631 kb on + strand at 654.703 kb on + strand at 654.718 kb on - strand at 654.720 kb on - strand at 654.722 kb on + strand at 654.727 kb on + strand at 654.727 kb on + strand at 654.727 kb on + strand at 654.728 kb on - strand at 654.735 kb on - strand at 654.740 kb on - strand at 654.772 kb on + strand at 654.800 kb on - strand at 654.853 kb on + strand at 654.897 kb on + strand at 654.932 kb on + strand at 654.949 kb on + strand at 654.968 kb on + strand at 655.004 kb on - strand at 655.028 kb on - strand, within BBR_RS12940 at 655.039 kb on + strand, within BBR_RS12940 at 655.052 kb on - strand, within BBR_RS12940 at 655.052 kb on - strand, within BBR_RS12940 at 655.065 kb on - strand, within BBR_RS12940 at 655.065 kb on - strand, within BBR_RS12940 at 655.082 kb on - strand, within BBR_RS12940 at 655.094 kb on + strand, within BBR_RS12940 at 655.143 kb on + strand, within BBR_RS12940 at 655.145 kb on + strand, within BBR_RS12940 at 655.145 kb on + strand, within BBR_RS12940 at 655.153 kb on - strand, within BBR_RS12940 at 655.160 kb on + strand, within BBR_RS12940 at 655.186 kb on - strand, within BBR_RS12940 at 655.192 kb on + strand, within BBR_RS12940 at 655.215 kb on + strand, within BBR_RS12940 at 655.299 kb on + strand, within BBR_RS12940 at 655.322 kb on + strand, within BBR_RS12940 at 655.322 kb on + strand, within BBR_RS12940 at 655.330 kb on - strand, within BBR_RS12940 at 655.330 kb on - strand, within BBR_RS12940 at 655.337 kb on - strand, within BBR_RS12940 at 655.366 kb on - strand, within BBR_RS12940 at 655.369 kb on + strand, within BBR_RS12940 at 655.379 kb on + strand, within BBR_RS12940 at 655.386 kb on + strand, within BBR_RS12940 at 655.386 kb on + strand, within BBR_RS12940 at 655.435 kb on + strand, within BBR_RS12940 at 655.455 kb on + strand, within BBR_RS12940 at 655.458 kb on - strand, within BBR_RS12940 at 655.463 kb on - strand, within BBR_RS12940 at 655.463 kb on - strand, within BBR_RS12940 at 655.468 kb on + strand, within BBR_RS12940 at 655.493 kb on + strand at 655.513 kb on + strand at 655.522 kb on - strand at 655.556 kb on - strand at 655.557 kb on + strand at 655.557 kb on + strand at 655.562 kb on + strand at 655.570 kb on - strand at 655.579 kb on + strand at 655.579 kb on + strand at 655.579 kb on + strand at 655.587 kb on - strand at 655.612 kb on - strand at 655.669 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Streptomycin 0.3 mM remove 653,977 + BBR_RS12935 0.39 -0.8 653,977 + BBR_RS12935 0.39 -1.3 653,977 + BBR_RS12935 0.39 +1.1 653,977 + BBR_RS12935 0.39 +0.8 653,977 + BBR_RS12935 0.39 -0.7 653,977 + BBR_RS12935 0.39 -1.1 653,985 - BBR_RS12935 0.39 +1.4 653,985 - BBR_RS12935 0.39 -0.7 654,052 - BBR_RS12935 0.46 -0.7 654,052 - BBR_RS12935 0.46 -1.6 654,084 - BBR_RS12935 0.50 -0.5 654,110 - BBR_RS12935 0.52 -0.9 654,123 + BBR_RS12935 0.54 -0.5 654,124 + BBR_RS12935 0.54 -0.3 654,124 - BBR_RS12935 0.54 +1.4 654,147 + BBR_RS12935 0.56 +0.7 654,147 + BBR_RS12935 0.56 -0.5 654,161 + BBR_RS12935 0.58 -0.7 654,190 + BBR_RS12935 0.61 +0.8 654,195 + BBR_RS12935 0.61 +0.0 654,195 - BBR_RS12935 0.61 +0.2 654,226 - BBR_RS12935 0.64 +0.2 654,240 - BBR_RS12935 0.66 +3.1 654,245 + BBR_RS12935 0.66 +0.3 654,260 - BBR_RS12935 0.68 +1.6 654,260 - BBR_RS12935 0.68 -0.5 654,262 + BBR_RS12935 0.68 -2.6 654,270 - BBR_RS12935 0.69 -0.3 654,312 + BBR_RS12935 0.73 -1.6 654,332 + BBR_RS12935 0.75 -0.2 654,332 + BBR_RS12935 0.75 +0.1 654,369 + BBR_RS12935 0.79 -0.5 654,377 + BBR_RS12935 0.80 -0.5 654,382 + BBR_RS12935 0.81 -0.7 654,387 + BBR_RS12935 0.81 +0.6 654,417 - BBR_RS12935 0.84 -2.1 654,480 - -1.6 654,535 - +1.8 654,539 + +0.9 654,555 + +1.8 654,577 + +0.4 654,577 + -1.2 654,577 + +1.6 654,577 + +0.8 654,577 + +1.6 654,577 + +0.6 654,585 - +0.3 654,585 - +0.0 654,585 - +0.7 654,585 - -0.2 654,587 - +1.9 654,587 - +2.0 654,587 - -0.3 654,611 - -0.9 654,611 - +0.6 654,620 - -0.4 654,620 - -0.9 654,626 - +0.6 654,631 + -0.6 654,703 + +0.3 654,718 - +0.8 654,720 - +0.1 654,722 + +0.5 654,727 + +0.8 654,727 + -1.9 654,727 + -0.8 654,728 - -2.2 654,735 - -0.1 654,740 - +1.7 654,772 + +0.3 654,800 - -0.4 654,853 + -0.6 654,897 + -1.2 654,932 + -0.5 654,949 + +1.1 654,968 + -1.3 655,004 - +0.3 655,028 - BBR_RS12940 0.10 -1.8 655,039 + BBR_RS12940 0.12 +1.1 655,052 - BBR_RS12940 0.14 +0.0 655,052 - BBR_RS12940 0.14 -3.1 655,065 - BBR_RS12940 0.17 +1.7 655,065 - BBR_RS12940 0.17 -2.4 655,082 - BBR_RS12940 0.20 -1.3 655,094 + BBR_RS12940 0.22 -1.2 655,143 + BBR_RS12940 0.30 -2.9 655,145 + BBR_RS12940 0.30 -0.0 655,145 + BBR_RS12940 0.30 -1.1 655,153 - BBR_RS12940 0.32 -0.0 655,160 + BBR_RS12940 0.33 -0.8 655,186 - BBR_RS12940 0.37 -1.3 655,192 + BBR_RS12940 0.38 -0.6 655,215 + BBR_RS12940 0.42 -0.2 655,299 + BBR_RS12940 0.57 -1.5 655,322 + BBR_RS12940 0.61 -2.8 655,322 + BBR_RS12940 0.61 +0.6 655,330 - BBR_RS12940 0.62 -0.3 655,330 - BBR_RS12940 0.62 -1.1 655,337 - BBR_RS12940 0.63 -1.4 655,366 - BBR_RS12940 0.68 -1.6 655,369 + BBR_RS12940 0.69 -1.6 655,379 + BBR_RS12940 0.71 -0.7 655,386 + BBR_RS12940 0.72 -1.3 655,386 + BBR_RS12940 0.72 -2.7 655,435 + BBR_RS12940 0.80 -0.1 655,455 + BBR_RS12940 0.84 -1.8 655,458 - BBR_RS12940 0.84 -0.8 655,463 - BBR_RS12940 0.85 +1.4 655,463 - BBR_RS12940 0.85 -2.0 655,468 + BBR_RS12940 0.86 -2.4 655,493 + -0.4 655,513 + -2.3 655,522 - -0.9 655,556 - +0.0 655,557 + -0.4 655,557 + -0.3 655,562 + -1.0 655,570 - -0.4 655,579 + +0.8 655,579 + -1.0 655,579 + +1.1 655,587 - -0.7 655,612 - +1.0 655,669 + -2.4
Or see this region's nucleotide sequence