Experiment: Recovery broth with Streptomycin 0.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS12595 and BBR_RS12600 are separated by 10 nucleotides BBR_RS12600 and BBR_RS12605 overlap by 8 nucleotides BBR_RS12605 and BBR_RS12610 overlap by 4 nucleotides BBR_RS12610 and BBR_RS20720 overlap by 14 nucleotides
BBR_RS12595: BBR_RS12595 - membrane spanning protein, at 582,391 to 583,617
_RS12595
BBR_RS12600: BBR_RS12600 - serine acetyltransferase, at 583,628 to 584,092
_RS12600
BBR_RS12605: BBR_RS12605 - glycosyltransferase family 2 protein, at 584,085 to 585,095
_RS12605
BBR_RS12610: BBR_RS12610 - capsular polysaccharide biosynthesis protein, at 585,092 to 586,027
_RS12610
BBR_RS20720: BBR_RS20720 - DUF4422 domain-containing protein, at 586,014 to 586,844
_RS20720
Position (kb) 584
585
586 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 583.729 kb on - strand, within BBR_RS12600 at 583.741 kb on + strand, within BBR_RS12600 at 583.763 kb on - strand, within BBR_RS12600 at 583.765 kb on - strand, within BBR_RS12600 at 583.767 kb on - strand, within BBR_RS12600 at 583.771 kb on + strand, within BBR_RS12600 at 583.798 kb on + strand, within BBR_RS12600 at 583.814 kb on - strand, within BBR_RS12600 at 583.898 kb on - strand, within BBR_RS12600 at 583.941 kb on - strand, within BBR_RS12600 at 583.980 kb on + strand, within BBR_RS12600 at 583.988 kb on - strand, within BBR_RS12600 at 584.002 kb on - strand, within BBR_RS12600 at 584.025 kb on - strand, within BBR_RS12600 at 584.034 kb on - strand, within BBR_RS12600 at 584.034 kb on - strand, within BBR_RS12600 at 584.090 kb on - strand at 584.090 kb on - strand at 584.150 kb on + strand at 584.171 kb on - strand at 584.201 kb on - strand, within BBR_RS12605 at 584.441 kb on - strand, within BBR_RS12605 at 584.474 kb on + strand, within BBR_RS12605 at 584.482 kb on - strand, within BBR_RS12605 at 584.482 kb on - strand, within BBR_RS12605 at 584.504 kb on + strand, within BBR_RS12605 at 584.565 kb on - strand, within BBR_RS12605 at 584.742 kb on - strand, within BBR_RS12605 at 585.106 kb on + strand at 585.197 kb on - strand, within BBR_RS12610 at 585.216 kb on - strand, within BBR_RS12610 at 585.274 kb on - strand, within BBR_RS12610 at 585.282 kb on - strand, within BBR_RS12610 at 585.342 kb on + strand, within BBR_RS12610 at 585.355 kb on - strand, within BBR_RS12610 at 585.434 kb on + strand, within BBR_RS12610 at 585.505 kb on + strand, within BBR_RS12610 at 585.605 kb on - strand, within BBR_RS12610 at 585.672 kb on - strand, within BBR_RS12610 at 585.769 kb on - strand, within BBR_RS12610 at 585.778 kb on + strand, within BBR_RS12610 at 585.786 kb on - strand, within BBR_RS12610 at 585.832 kb on + strand, within BBR_RS12610 at 585.896 kb on - strand, within BBR_RS12610 at 585.953 kb on - strand at 585.953 kb on - strand at 585.973 kb on - strand at 585.981 kb on + strand at 585.989 kb on - strand at 585.989 kb on - strand at 585.991 kb on - strand at 586.002 kb on + strand at 586.010 kb on - strand at 586.010 kb on - strand at 586.010 kb on - strand at 586.010 kb on - strand at 586.010 kb on - strand at 586.043 kb on - strand at 586.056 kb on - strand at 586.061 kb on + strand at 586.083 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Streptomycin 0.3 mM remove 583,729 - BBR_RS12600 0.22 -1.4 583,741 + BBR_RS12600 0.24 +0.7 583,763 - BBR_RS12600 0.29 +0.1 583,765 - BBR_RS12600 0.29 +0.9 583,767 - BBR_RS12600 0.30 +0.3 583,771 + BBR_RS12600 0.31 +0.3 583,798 + BBR_RS12600 0.37 +1.2 583,814 - BBR_RS12600 0.40 -0.3 583,898 - BBR_RS12600 0.58 +0.8 583,941 - BBR_RS12600 0.67 -0.6 583,980 + BBR_RS12600 0.76 -1.1 583,988 - BBR_RS12600 0.77 +1.7 584,002 - BBR_RS12600 0.80 -1.0 584,025 - BBR_RS12600 0.85 +1.2 584,034 - BBR_RS12600 0.87 -0.9 584,034 - BBR_RS12600 0.87 +0.1 584,090 - -0.2 584,090 - +0.1 584,150 + -0.5 584,171 - +1.1 584,201 - BBR_RS12605 0.11 -2.1 584,441 - BBR_RS12605 0.35 -2.8 584,474 + BBR_RS12605 0.38 -1.7 584,482 - BBR_RS12605 0.39 -0.3 584,482 - BBR_RS12605 0.39 -0.5 584,504 + BBR_RS12605 0.41 -0.8 584,565 - BBR_RS12605 0.47 -1.2 584,742 - BBR_RS12605 0.65 +1.2 585,106 + +2.0 585,197 - BBR_RS12610 0.11 +2.4 585,216 - BBR_RS12610 0.13 +1.2 585,274 - BBR_RS12610 0.19 -0.8 585,282 - BBR_RS12610 0.20 +0.2 585,342 + BBR_RS12610 0.27 +1.5 585,355 - BBR_RS12610 0.28 +0.2 585,434 + BBR_RS12610 0.37 -0.3 585,505 + BBR_RS12610 0.44 +0.4 585,605 - BBR_RS12610 0.55 +0.1 585,672 - BBR_RS12610 0.62 +0.0 585,769 - BBR_RS12610 0.72 -1.1 585,778 + BBR_RS12610 0.73 +0.4 585,786 - BBR_RS12610 0.74 -0.0 585,832 + BBR_RS12610 0.79 +1.9 585,896 - BBR_RS12610 0.86 +0.1 585,953 - -0.6 585,953 - -0.5 585,973 - -0.2 585,981 + -1.4 585,989 - -0.1 585,989 - +0.4 585,991 - -2.4 586,002 + -1.4 586,010 - +1.0 586,010 - +1.0 586,010 - -0.0 586,010 - -2.2 586,010 - +0.3 586,043 - -2.3 586,056 - +3.1 586,061 + +1.0 586,083 - -1.9
Or see this region's nucleotide sequence