Experiment: Recovery broth with Streptomycin 0.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS12455 and BBR_RS12460 are separated by 161 nucleotides BBR_RS12460 and BBR_RS20700 are separated by 163 nucleotides BBR_RS20700 and BBR_RS12470 are separated by 281 nucleotides
BBR_RS12455: BBR_RS12455 - ribonuclease HII, at 543,739 to 544,596
_RS12455
BBR_RS12460: BBR_RS12460 - LacI family DNA-binding transcriptional regulator, at 544,758 to 545,873
_RS12460
BBR_RS20700: BBR_RS20700 - ribulose phosphate epimerase, at 546,037 to 546,156
_RS20700
BBR_RS12470: BBR_RS12470 - long-chain fatty acid--CoA ligase, at 546,438 to 548,261
_RS12470
Position (kb) 544
545
546 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 543.793 kb on - strand at 543.834 kb on - strand, within BBR_RS12455 at 543.964 kb on + strand, within BBR_RS12455 at 543.964 kb on + strand, within BBR_RS12455 at 543.964 kb on + strand, within BBR_RS12455 at 543.964 kb on + strand, within BBR_RS12455 at 543.964 kb on + strand, within BBR_RS12455 at 543.982 kb on - strand, within BBR_RS12455 at 543.996 kb on + strand, within BBR_RS12455 at 543.996 kb on + strand, within BBR_RS12455 at 543.996 kb on + strand, within BBR_RS12455 at 544.065 kb on + strand, within BBR_RS12455 at 544.065 kb on + strand, within BBR_RS12455 at 544.073 kb on - strand, within BBR_RS12455 at 544.073 kb on - strand, within BBR_RS12455 at 544.073 kb on - strand, within BBR_RS12455 at 544.073 kb on - strand, within BBR_RS12455 at 544.082 kb on - strand, within BBR_RS12455 at 544.082 kb on - strand, within BBR_RS12455 at 544.082 kb on - strand, within BBR_RS12455 at 544.084 kb on + strand, within BBR_RS12455 at 544.084 kb on + strand, within BBR_RS12455 at 544.092 kb on - strand, within BBR_RS12455 at 544.092 kb on - strand, within BBR_RS12455 at 544.101 kb on + strand, within BBR_RS12455 at 544.101 kb on + strand, within BBR_RS12455 at 544.109 kb on - strand, within BBR_RS12455 at 544.136 kb on + strand, within BBR_RS12455 at 544.138 kb on - strand, within BBR_RS12455 at 544.150 kb on - strand, within BBR_RS12455 at 544.154 kb on - strand, within BBR_RS12455 at 544.164 kb on - strand, within BBR_RS12455 at 544.170 kb on + strand, within BBR_RS12455 at 544.176 kb on + strand, within BBR_RS12455 at 544.178 kb on - strand, within BBR_RS12455 at 544.181 kb on + strand, within BBR_RS12455 at 544.181 kb on + strand, within BBR_RS12455 at 544.187 kb on + strand, within BBR_RS12455 at 544.224 kb on - strand, within BBR_RS12455 at 544.232 kb on + strand, within BBR_RS12455 at 544.232 kb on + strand, within BBR_RS12455 at 544.232 kb on + strand, within BBR_RS12455 at 544.232 kb on + strand, within BBR_RS12455 at 544.232 kb on + strand, within BBR_RS12455 at 544.232 kb on + strand, within BBR_RS12455 at 544.254 kb on - strand, within BBR_RS12455 at 544.256 kb on - strand, within BBR_RS12455 at 544.256 kb on - strand, within BBR_RS12455 at 544.266 kb on + strand, within BBR_RS12455 at 544.272 kb on + strand, within BBR_RS12455 at 544.301 kb on + strand, within BBR_RS12455 at 544.324 kb on + strand, within BBR_RS12455 at 544.326 kb on - strand, within BBR_RS12455 at 544.334 kb on + strand, within BBR_RS12455 at 544.337 kb on - strand, within BBR_RS12455 at 544.380 kb on + strand, within BBR_RS12455 at 544.380 kb on + strand, within BBR_RS12455 at 544.380 kb on + strand, within BBR_RS12455 at 544.381 kb on + strand, within BBR_RS12455 at 544.388 kb on - strand, within BBR_RS12455 at 544.395 kb on + strand, within BBR_RS12455 at 544.398 kb on - strand, within BBR_RS12455 at 544.403 kb on - strand, within BBR_RS12455 at 544.415 kb on + strand, within BBR_RS12455 at 544.425 kb on - strand, within BBR_RS12455 at 544.448 kb on + strand, within BBR_RS12455 at 544.460 kb on + strand, within BBR_RS12455 at 544.460 kb on + strand, within BBR_RS12455 at 544.466 kb on - strand, within BBR_RS12455 at 544.499 kb on - strand, within BBR_RS12455 at 544.503 kb on - strand, within BBR_RS12455 at 544.506 kb on + strand, within BBR_RS12455 at 544.512 kb on + strand at 544.521 kb on - strand at 544.521 kb on - strand at 544.554 kb on - strand at 544.564 kb on + strand at 544.570 kb on - strand at 544.570 kb on - strand at 544.598 kb on - strand at 544.608 kb on + strand at 544.608 kb on + strand at 544.611 kb on - strand at 544.624 kb on + strand at 544.713 kb on - strand at 544.725 kb on + strand at 544.739 kb on + strand at 544.746 kb on + strand at 544.805 kb on + strand at 544.833 kb on + strand at 544.833 kb on + strand at 544.841 kb on - strand at 544.866 kb on + strand at 544.866 kb on - strand at 544.868 kb on + strand at 544.868 kb on + strand at 544.895 kb on - strand, within BBR_RS12460 at 544.900 kb on + strand, within BBR_RS12460 at 544.918 kb on - strand, within BBR_RS12460 at 544.918 kb on - strand, within BBR_RS12460 at 544.933 kb on - strand, within BBR_RS12460 at 544.955 kb on - strand, within BBR_RS12460 at 544.995 kb on - strand, within BBR_RS12460 at 545.044 kb on + strand, within BBR_RS12460 at 545.044 kb on + strand, within BBR_RS12460 at 545.052 kb on - strand, within BBR_RS12460 at 545.054 kb on + strand, within BBR_RS12460 at 545.083 kb on - strand, within BBR_RS12460 at 545.096 kb on + strand, within BBR_RS12460 at 545.096 kb on + strand, within BBR_RS12460 at 545.122 kb on + strand, within BBR_RS12460 at 545.132 kb on + strand, within BBR_RS12460 at 545.137 kb on + strand, within BBR_RS12460 at 545.138 kb on - strand, within BBR_RS12460 at 545.177 kb on - strand, within BBR_RS12460 at 545.177 kb on - strand, within BBR_RS12460 at 545.186 kb on + strand, within BBR_RS12460 at 545.192 kb on - strand, within BBR_RS12460 at 545.268 kb on - strand, within BBR_RS12460 at 545.291 kb on + strand, within BBR_RS12460 at 545.303 kb on + strand, within BBR_RS12460 at 545.313 kb on + strand, within BBR_RS12460 at 545.318 kb on + strand, within BBR_RS12460 at 545.320 kb on + strand, within BBR_RS12460 at 545.338 kb on - strand, within BBR_RS12460 at 545.345 kb on - strand, within BBR_RS12460 at 545.371 kb on - strand, within BBR_RS12460 at 545.371 kb on - strand, within BBR_RS12460 at 545.375 kb on + strand, within BBR_RS12460 at 545.378 kb on + strand, within BBR_RS12460 at 545.419 kb on + strand, within BBR_RS12460 at 545.420 kb on + strand, within BBR_RS12460 at 545.469 kb on + strand, within BBR_RS12460 at 545.477 kb on + strand, within BBR_RS12460 at 545.479 kb on + strand, within BBR_RS12460 at 545.483 kb on + strand, within BBR_RS12460 at 545.547 kb on + strand, within BBR_RS12460 at 545.563 kb on + strand, within BBR_RS12460 at 545.563 kb on + strand, within BBR_RS12460 at 545.624 kb on - strand, within BBR_RS12460 at 545.682 kb on + strand, within BBR_RS12460 at 545.686 kb on + strand, within BBR_RS12460 at 545.687 kb on + strand, within BBR_RS12460 at 545.695 kb on - strand, within BBR_RS12460 at 545.707 kb on - strand, within BBR_RS12460 at 545.742 kb on - strand, within BBR_RS12460 at 545.763 kb on - strand at 545.775 kb on - strand at 545.798 kb on - strand at 545.814 kb on + strand at 545.839 kb on - strand at 545.848 kb on + strand at 545.854 kb on + strand at 545.857 kb on - strand at 545.929 kb on + strand at 545.929 kb on + strand at 545.934 kb on + strand at 545.934 kb on + strand at 545.934 kb on + strand at 545.937 kb on + strand at 545.937 kb on - strand at 545.981 kb on + strand at 545.981 kb on + strand at 545.999 kb on - strand at 546.004 kb on - strand at 546.123 kb on - strand, within BBR_RS20700 at 546.131 kb on - strand, within BBR_RS20700 at 546.131 kb on - strand, within BBR_RS20700 at 546.134 kb on + strand, within BBR_RS20700 at 546.146 kb on + strand at 546.146 kb on + strand at 546.166 kb on + strand at 546.166 kb on + strand at 546.181 kb on + strand at 546.186 kb on - strand at 546.222 kb on + strand at 546.232 kb on + strand at 546.232 kb on + strand at 546.247 kb on + strand at 546.249 kb on - strand at 546.266 kb on + strand at 546.274 kb on - strand at 546.284 kb on + strand at 546.340 kb on + strand at 546.348 kb on + strand at 546.348 kb on + strand at 546.356 kb on - strand at 546.378 kb on - strand at 546.381 kb on - strand at 546.421 kb on + strand at 546.421 kb on + strand at 546.421 kb on + strand at 546.473 kb on + strand at 546.497 kb on - strand at 546.497 kb on - strand at 546.497 kb on - strand at 546.532 kb on + strand at 546.535 kb on - strand at 546.549 kb on - strand at 546.549 kb on - strand at 546.614 kb on - strand at 546.621 kb on - strand, within BBR_RS12470 at 546.646 kb on + strand, within BBR_RS12470 at 546.661 kb on + strand, within BBR_RS12470 at 546.671 kb on - strand, within BBR_RS12470 at 546.684 kb on - strand, within BBR_RS12470 at 546.694 kb on - strand, within BBR_RS12470 at 546.698 kb on - strand, within BBR_RS12470 at 546.709 kb on - strand, within BBR_RS12470 at 546.716 kb on + strand, within BBR_RS12470 at 546.727 kb on + strand, within BBR_RS12470 at 546.727 kb on + strand, within BBR_RS12470 at 546.727 kb on + strand, within BBR_RS12470 at 546.735 kb on - strand, within BBR_RS12470 at 546.735 kb on - strand, within BBR_RS12470 at 546.756 kb on - strand, within BBR_RS12470 at 546.767 kb on + strand, within BBR_RS12470 at 546.767 kb on - strand, within BBR_RS12470 at 546.767 kb on - strand, within BBR_RS12470 at 546.775 kb on - strand, within BBR_RS12470 at 546.787 kb on + strand, within BBR_RS12470 at 546.791 kb on + strand, within BBR_RS12470 at 546.795 kb on - strand, within BBR_RS12470 at 546.812 kb on - strand, within BBR_RS12470 at 546.817 kb on - strand, within BBR_RS12470 at 546.818 kb on + strand, within BBR_RS12470
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Streptomycin 0.3 mM remove 543,793 - +0.4 543,834 - BBR_RS12455 0.11 +0.1 543,964 + BBR_RS12455 0.26 -2.0 543,964 + BBR_RS12455 0.26 -2.5 543,964 + BBR_RS12455 0.26 -0.1 543,964 + BBR_RS12455 0.26 +1.0 543,964 + BBR_RS12455 0.26 -0.9 543,982 - BBR_RS12455 0.28 +0.9 543,996 + BBR_RS12455 0.30 -1.7 543,996 + BBR_RS12455 0.30 +0.5 543,996 + BBR_RS12455 0.30 -2.4 544,065 + BBR_RS12455 0.38 -0.9 544,065 + BBR_RS12455 0.38 -1.4 544,073 - BBR_RS12455 0.39 +0.4 544,073 - BBR_RS12455 0.39 -1.1 544,073 - BBR_RS12455 0.39 -0.5 544,073 - BBR_RS12455 0.39 -0.2 544,082 - BBR_RS12455 0.40 +0.8 544,082 - BBR_RS12455 0.40 -0.6 544,082 - BBR_RS12455 0.40 -1.3 544,084 + BBR_RS12455 0.40 -0.1 544,084 + BBR_RS12455 0.40 -0.2 544,092 - BBR_RS12455 0.41 -1.9 544,092 - BBR_RS12455 0.41 +0.0 544,101 + BBR_RS12455 0.42 -0.8 544,101 + BBR_RS12455 0.42 -2.0 544,109 - BBR_RS12455 0.43 +1.0 544,136 + BBR_RS12455 0.46 +0.5 544,138 - BBR_RS12455 0.47 -0.8 544,150 - BBR_RS12455 0.48 -1.3 544,154 - BBR_RS12455 0.48 +0.0 544,164 - BBR_RS12455 0.50 +0.0 544,170 + BBR_RS12455 0.50 +0.3 544,176 + BBR_RS12455 0.51 +0.3 544,178 - BBR_RS12455 0.51 -1.0 544,181 + BBR_RS12455 0.52 -1.1 544,181 + BBR_RS12455 0.52 -0.1 544,187 + BBR_RS12455 0.52 +0.8 544,224 - BBR_RS12455 0.57 -1.0 544,232 + BBR_RS12455 0.57 +2.0 544,232 + BBR_RS12455 0.57 -0.4 544,232 + BBR_RS12455 0.57 +0.7 544,232 + BBR_RS12455 0.57 -1.0 544,232 + BBR_RS12455 0.57 -0.3 544,232 + BBR_RS12455 0.57 +1.0 544,254 - BBR_RS12455 0.60 +0.2 544,256 - BBR_RS12455 0.60 -1.8 544,256 - BBR_RS12455 0.60 +0.7 544,266 + BBR_RS12455 0.61 +0.3 544,272 + BBR_RS12455 0.62 +0.1 544,301 + BBR_RS12455 0.66 -0.2 544,324 + BBR_RS12455 0.68 +1.1 544,326 - BBR_RS12455 0.68 +1.0 544,334 + BBR_RS12455 0.69 -0.8 544,337 - BBR_RS12455 0.70 +1.0 544,380 + BBR_RS12455 0.75 -0.8 544,380 + BBR_RS12455 0.75 +1.1 544,380 + BBR_RS12455 0.75 -0.6 544,381 + BBR_RS12455 0.75 -1.6 544,388 - BBR_RS12455 0.76 -1.3 544,395 + BBR_RS12455 0.76 -1.8 544,398 - BBR_RS12455 0.77 +0.6 544,403 - BBR_RS12455 0.77 -0.7 544,415 + BBR_RS12455 0.79 -0.8 544,425 - BBR_RS12455 0.80 +1.0 544,448 + BBR_RS12455 0.83 -2.5 544,460 + BBR_RS12455 0.84 -2.1 544,460 + BBR_RS12455 0.84 -1.0 544,466 - BBR_RS12455 0.85 -1.8 544,499 - BBR_RS12455 0.89 +0.1 544,503 - BBR_RS12455 0.89 -0.1 544,506 + BBR_RS12455 0.89 -0.6 544,512 + +0.7 544,521 - +1.8 544,521 - -1.9 544,554 - -1.5 544,564 + +1.2 544,570 - +1.8 544,570 - +0.7 544,598 - +0.4 544,608 + +0.3 544,608 + -0.2 544,611 - -0.6 544,624 + -0.2 544,713 - -0.2 544,725 + +0.4 544,739 + +0.0 544,746 + -1.1 544,805 + +0.3 544,833 + +0.9 544,833 + -0.3 544,841 - -0.7 544,866 + +1.4 544,866 - -1.5 544,868 + +1.7 544,868 + -1.4 544,895 - BBR_RS12460 0.12 -0.7 544,900 + BBR_RS12460 0.13 -1.2 544,918 - BBR_RS12460 0.14 +0.1 544,918 - BBR_RS12460 0.14 -1.0 544,933 - BBR_RS12460 0.16 -0.7 544,955 - BBR_RS12460 0.18 +1.4 544,995 - BBR_RS12460 0.21 -0.4 545,044 + BBR_RS12460 0.26 -1.6 545,044 + BBR_RS12460 0.26 -0.9 545,052 - BBR_RS12460 0.26 +1.1 545,054 + BBR_RS12460 0.27 -1.3 545,083 - BBR_RS12460 0.29 +1.4 545,096 + BBR_RS12460 0.30 +0.1 545,096 + BBR_RS12460 0.30 -0.7 545,122 + BBR_RS12460 0.33 -2.6 545,132 + BBR_RS12460 0.34 +0.1 545,137 + BBR_RS12460 0.34 -0.1 545,138 - BBR_RS12460 0.34 +1.4 545,177 - BBR_RS12460 0.38 -0.5 545,177 - BBR_RS12460 0.38 -0.2 545,186 + BBR_RS12460 0.38 +1.4 545,192 - BBR_RS12460 0.39 -1.0 545,268 - BBR_RS12460 0.46 -0.6 545,291 + BBR_RS12460 0.48 -0.2 545,303 + BBR_RS12460 0.49 -0.7 545,313 + BBR_RS12460 0.50 -2.1 545,318 + BBR_RS12460 0.50 -0.7 545,320 + BBR_RS12460 0.50 +0.0 545,338 - BBR_RS12460 0.52 -0.0 545,345 - BBR_RS12460 0.53 +1.2 545,371 - BBR_RS12460 0.55 +1.7 545,371 - BBR_RS12460 0.55 +2.2 545,375 + BBR_RS12460 0.55 -1.6 545,378 + BBR_RS12460 0.56 +0.3 545,419 + BBR_RS12460 0.59 -0.9 545,420 + BBR_RS12460 0.59 -0.4 545,469 + BBR_RS12460 0.64 -0.0 545,477 + BBR_RS12460 0.64 -0.5 545,479 + BBR_RS12460 0.65 -0.7 545,483 + BBR_RS12460 0.65 +0.7 545,547 + BBR_RS12460 0.71 +1.4 545,563 + BBR_RS12460 0.72 -0.3 545,563 + BBR_RS12460 0.72 -0.9 545,624 - BBR_RS12460 0.78 -0.1 545,682 + BBR_RS12460 0.83 +0.9 545,686 + BBR_RS12460 0.83 +0.7 545,687 + BBR_RS12460 0.83 -1.0 545,695 - BBR_RS12460 0.84 -0.0 545,707 - BBR_RS12460 0.85 +0.2 545,742 - BBR_RS12460 0.88 -2.6 545,763 - -0.8 545,775 - -0.0 545,798 - +1.7 545,814 + +0.9 545,839 - +0.7 545,848 + -0.3 545,854 + -1.6 545,857 - +0.5 545,929 + -0.9 545,929 + -0.1 545,934 + -2.9 545,934 + +1.3 545,934 + +0.8 545,937 + +1.2 545,937 - -0.9 545,981 + +0.1 545,981 + +0.1 545,999 - -2.0 546,004 - +0.4 546,123 - BBR_RS20700 0.72 +1.6 546,131 - BBR_RS20700 0.78 -2.6 546,131 - BBR_RS20700 0.78 -0.2 546,134 + BBR_RS20700 0.81 -0.4 546,146 + -0.1 546,146 + -1.5 546,166 + -1.6 546,166 + -1.0 546,181 + -1.0 546,186 - +0.7 546,222 + +1.7 546,232 + -0.4 546,232 + +0.7 546,247 + +0.5 546,249 - -2.5 546,266 + -0.6 546,274 - -1.9 546,284 + -0.5 546,340 + -0.8 546,348 + +0.2 546,348 + +0.7 546,356 - -0.3 546,378 - -0.8 546,381 - +0.6 546,421 + -0.7 546,421 + -2.2 546,421 + +0.6 546,473 + -0.5 546,497 - +0.4 546,497 - -1.1 546,497 - +0.8 546,532 + +4.0 546,535 - +1.1 546,549 - -1.8 546,549 - -1.3 546,614 - -1.0 546,621 - BBR_RS12470 0.10 -0.4 546,646 + BBR_RS12470 0.11 -1.4 546,661 + BBR_RS12470 0.12 -0.5 546,671 - BBR_RS12470 0.13 +1.6 546,684 - BBR_RS12470 0.13 -2.5 546,694 - BBR_RS12470 0.14 +0.5 546,698 - BBR_RS12470 0.14 -1.2 546,709 - BBR_RS12470 0.15 -2.2 546,716 + BBR_RS12470 0.15 -2.2 546,727 + BBR_RS12470 0.16 -0.3 546,727 + BBR_RS12470 0.16 +0.4 546,727 + BBR_RS12470 0.16 -0.8 546,735 - BBR_RS12470 0.16 -0.8 546,735 - BBR_RS12470 0.16 -0.4 546,756 - BBR_RS12470 0.17 -1.7 546,767 + BBR_RS12470 0.18 -0.5 546,767 - BBR_RS12470 0.18 -0.2 546,767 - BBR_RS12470 0.18 -0.1 546,775 - BBR_RS12470 0.18 -0.8 546,787 + BBR_RS12470 0.19 -0.5 546,791 + BBR_RS12470 0.19 -1.4 546,795 - BBR_RS12470 0.20 -0.3 546,812 - BBR_RS12470 0.21 -1.0 546,817 - BBR_RS12470 0.21 +0.8 546,818 + BBR_RS12470 0.21 +1.6
Or see this region's nucleotide sequence