Experiment: Recovery broth with Streptomycin 0.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS11795 and BBR_RS11800 are separated by 175 nucleotides BBR_RS11800 and BBR_RS11805 are separated by 59 nucleotides BBR_RS11805 and BBR_RS11810 are separated by 233 nucleotides BBR_RS11810 and BBR_RS11815 are separated by 85 nucleotides
BBR_RS11795: BBR_RS11795 - DNA-binding response regulator, at 405,623 to 406,393
_RS11795
BBR_RS11800: BBR_RS11800 - hypothetical protein, at 406,569 to 407,273
_RS11800
BBR_RS11805: BBR_RS11805 - hypothetical protein, at 407,333 to 407,911
_RS11805
BBR_RS11810: BBR_RS11810 - inorganic pyrophosphatase, at 408,145 to 408,639
_RS11810
BBR_RS11815: BBR_RS11815 - DUF3416 domain-containing protein, at 408,725 to 410,953
_RS11815
Position (kb) 407
408 Strain fitness (log2 ratio) -2
-1
0
1
2 at 406.411 kb on + strand at 406.411 kb on + strand at 406.419 kb on - strand at 406.420 kb on + strand at 406.424 kb on + strand at 406.428 kb on - strand at 406.428 kb on - strand at 406.467 kb on - strand at 406.502 kb on - strand at 406.517 kb on - strand at 406.520 kb on - strand at 406.520 kb on - strand at 406.520 kb on - strand at 407.261 kb on - strand at 407.271 kb on - strand at 407.281 kb on - strand at 407.281 kb on - strand at 407.305 kb on - strand at 407.305 kb on - strand at 407.323 kb on + strand at 407.326 kb on + strand at 407.331 kb on - strand at 407.331 kb on - strand at 407.360 kb on + strand at 407.362 kb on + strand at 407.368 kb on + strand at 407.408 kb on + strand, within BBR_RS11805 at 407.448 kb on + strand, within BBR_RS11805 at 407.468 kb on + strand, within BBR_RS11805 at 407.485 kb on + strand, within BBR_RS11805 at 407.497 kb on - strand, within BBR_RS11805 at 407.528 kb on - strand, within BBR_RS11805 at 407.553 kb on - strand, within BBR_RS11805 at 407.721 kb on - strand, within BBR_RS11805 at 407.736 kb on + strand, within BBR_RS11805 at 407.781 kb on - strand, within BBR_RS11805 at 407.790 kb on + strand, within BBR_RS11805 at 407.809 kb on - strand, within BBR_RS11805 at 407.809 kb on - strand, within BBR_RS11805 at 407.826 kb on + strand, within BBR_RS11805 at 407.826 kb on + strand, within BBR_RS11805 at 407.832 kb on - strand, within BBR_RS11805 at 407.833 kb on - strand, within BBR_RS11805 at 407.848 kb on - strand, within BBR_RS11805 at 407.879 kb on + strand at 407.882 kb on + strand at 407.887 kb on - strand at 407.889 kb on + strand at 407.896 kb on - strand at 407.907 kb on + strand at 407.939 kb on + strand at 407.939 kb on + strand at 407.954 kb on - strand at 407.964 kb on - strand at 407.970 kb on - strand at 407.977 kb on + strand at 408.000 kb on + strand at 408.008 kb on + strand at 408.008 kb on - strand at 408.008 kb on - strand at 408.008 kb on - strand at 408.010 kb on - strand at 408.016 kb on - strand at 408.044 kb on - strand at 408.052 kb on + strand at 408.070 kb on + strand at 408.072 kb on + strand at 408.080 kb on - strand at 408.140 kb on - strand at 408.670 kb on - strand at 408.670 kb on - strand at 408.670 kb on - strand at 408.672 kb on - strand at 408.673 kb on + strand at 408.676 kb on + strand at 408.676 kb on + strand at 408.676 kb on + strand at 408.676 kb on + strand at 408.676 kb on + strand at 408.676 kb on + strand at 408.676 kb on + strand at 408.678 kb on + strand at 408.678 kb on + strand at 408.680 kb on + strand at 408.684 kb on - strand at 408.684 kb on - strand at 408.684 kb on - strand at 408.684 kb on - strand at 408.684 kb on - strand at 408.684 kb on - strand at 408.684 kb on - strand at 408.684 kb on - strand at 408.686 kb on - strand at 408.686 kb on - strand at 408.686 kb on - strand at 408.693 kb on + strand at 408.699 kb on + strand at 408.703 kb on - strand at 408.707 kb on - strand at 408.707 kb on - strand at 408.707 kb on - strand at 408.707 kb on - strand at 408.707 kb on - strand at 408.707 kb on - strand at 408.707 kb on - strand at 408.707 kb on - strand at 408.716 kb on + strand at 408.790 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Streptomycin 0.3 mM remove 406,411 + -1.3 406,411 + +2.1 406,419 - -1.1 406,420 + +1.8 406,424 + -1.0 406,428 - -0.6 406,428 - -0.2 406,467 - -2.7 406,502 - +0.1 406,517 - -0.2 406,520 - -0.4 406,520 - +0.6 406,520 - -1.1 407,261 - +1.2 407,271 - +1.3 407,281 - +0.3 407,281 - +0.1 407,305 - +0.4 407,305 - +1.0 407,323 + +0.9 407,326 + +0.9 407,331 - +1.6 407,331 - -1.6 407,360 + -0.6 407,362 + +1.1 407,368 + -0.5 407,408 + BBR_RS11805 0.13 +1.0 407,448 + BBR_RS11805 0.20 -1.4 407,468 + BBR_RS11805 0.23 -1.1 407,485 + BBR_RS11805 0.26 +0.0 407,497 - BBR_RS11805 0.28 +0.4 407,528 - BBR_RS11805 0.34 -2.4 407,553 - BBR_RS11805 0.38 -1.0 407,721 - BBR_RS11805 0.67 -0.8 407,736 + BBR_RS11805 0.70 -0.8 407,781 - BBR_RS11805 0.77 -0.4 407,790 + BBR_RS11805 0.79 -0.8 407,809 - BBR_RS11805 0.82 -1.9 407,809 - BBR_RS11805 0.82 +0.1 407,826 + BBR_RS11805 0.85 -0.6 407,826 + BBR_RS11805 0.85 -0.9 407,832 - BBR_RS11805 0.86 -0.4 407,833 - BBR_RS11805 0.86 +0.3 407,848 - BBR_RS11805 0.89 -0.9 407,879 + -0.6 407,882 + -1.3 407,887 - -0.5 407,889 + -0.2 407,896 - +0.3 407,907 + +2.4 407,939 + -1.2 407,939 + -0.2 407,954 - -0.6 407,964 - +1.3 407,970 - -0.6 407,977 + -0.5 408,000 + -1.7 408,008 + +0.1 408,008 - +0.1 408,008 - -1.5 408,008 - -0.2 408,010 - -1.4 408,016 - +0.6 408,044 - +2.0 408,052 + +1.0 408,070 + +0.5 408,072 + -2.1 408,080 - +0.5 408,140 - +0.3 408,670 - +0.1 408,670 - -1.0 408,670 - +0.2 408,672 - -0.1 408,673 + -0.3 408,676 + -0.7 408,676 + +0.1 408,676 + +2.2 408,676 + +1.3 408,676 + +1.5 408,676 + -1.8 408,676 + -1.2 408,678 + -0.4 408,678 + -1.3 408,680 + -0.6 408,684 - +0.2 408,684 - +0.1 408,684 - -1.4 408,684 - -0.1 408,684 - +0.1 408,684 - -0.6 408,684 - +1.6 408,684 - -0.5 408,686 - -0.1 408,686 - -0.4 408,686 - -0.4 408,693 + -2.4 408,699 + -1.1 408,703 - -1.4 408,707 - -0.3 408,707 - -1.4 408,707 - +0.3 408,707 - -2.7 408,707 - -1.1 408,707 - -0.7 408,707 - -1.2 408,707 - -0.6 408,716 + +0.5 408,790 - +0.6
Or see this region's nucleotide sequence