Experiment: Recovery broth with Streptomycin 0.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS10575 and BBR_RS10580 are separated by 529 nucleotides BBR_RS10580 and BBR_RS10585 are separated by 88 nucleotides BBR_RS10585 and BBR_RS10590 overlap by 4 nucleotides
BBR_RS10575: BBR_RS10575 - rhomboid family intramembrane serine protease, at 90,455 to 91,195
_RS10575
BBR_RS10580: BBR_RS10580 - septation inhibitor protein, at 91,725 to 92,186
_RS10580
BBR_RS10585: BBR_RS10585 - DUF881 domain-containing protein, at 92,275 to 93,063
_RS10585
BBR_RS10590: BBR_RS10590 - class E sortase, at 93,060 to 94,265
_RS10590
Position (kb) 91
92
93 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 90.729 kb on - strand, within BBR_RS10575 at 90.729 kb on - strand, within BBR_RS10575 at 90.729 kb on - strand, within BBR_RS10575 at 90.729 kb on - strand, within BBR_RS10575 at 90.729 kb on - strand, within BBR_RS10575 at 90.729 kb on - strand, within BBR_RS10575 at 90.732 kb on + strand, within BBR_RS10575 at 90.732 kb on + strand, within BBR_RS10575 at 90.737 kb on + strand, within BBR_RS10575 at 90.740 kb on - strand, within BBR_RS10575 at 90.756 kb on - strand, within BBR_RS10575 at 90.768 kb on + strand, within BBR_RS10575 at 90.782 kb on - strand, within BBR_RS10575 at 90.786 kb on + strand, within BBR_RS10575 at 90.808 kb on + strand, within BBR_RS10575 at 90.810 kb on + strand, within BBR_RS10575 at 90.810 kb on + strand, within BBR_RS10575 at 90.810 kb on + strand, within BBR_RS10575 at 90.816 kb on - strand, within BBR_RS10575 at 90.816 kb on - strand, within BBR_RS10575 at 90.816 kb on - strand, within BBR_RS10575 at 90.816 kb on - strand, within BBR_RS10575 at 90.816 kb on - strand, within BBR_RS10575 at 90.817 kb on + strand, within BBR_RS10575 at 90.817 kb on + strand, within BBR_RS10575 at 90.817 kb on + strand, within BBR_RS10575 at 90.817 kb on + strand, within BBR_RS10575 at 90.818 kb on - strand, within BBR_RS10575 at 90.824 kb on - strand, within BBR_RS10575 at 90.825 kb on - strand, within BBR_RS10575 at 90.827 kb on + strand, within BBR_RS10575 at 90.827 kb on + strand, within BBR_RS10575 at 90.827 kb on + strand, within BBR_RS10575 at 90.827 kb on + strand, within BBR_RS10575 at 90.835 kb on - strand, within BBR_RS10575 at 90.835 kb on - strand, within BBR_RS10575 at 90.835 kb on - strand, within BBR_RS10575 at 90.842 kb on + strand, within BBR_RS10575 at 90.850 kb on - strand, within BBR_RS10575 at 90.871 kb on + strand, within BBR_RS10575 at 90.881 kb on - strand, within BBR_RS10575 at 90.886 kb on + strand, within BBR_RS10575 at 90.907 kb on + strand, within BBR_RS10575 at 90.932 kb on + strand, within BBR_RS10575 at 90.942 kb on - strand, within BBR_RS10575 at 90.950 kb on - strand, within BBR_RS10575 at 90.955 kb on - strand, within BBR_RS10575 at 90.960 kb on - strand, within BBR_RS10575 at 90.962 kb on + strand, within BBR_RS10575 at 90.962 kb on + strand, within BBR_RS10575 at 90.977 kb on - strand, within BBR_RS10575 at 90.979 kb on + strand, within BBR_RS10575 at 90.979 kb on + strand, within BBR_RS10575 at 90.981 kb on + strand, within BBR_RS10575 at 91.008 kb on - strand, within BBR_RS10575 at 91.008 kb on - strand, within BBR_RS10575 at 91.023 kb on - strand, within BBR_RS10575 at 91.028 kb on + strand, within BBR_RS10575 at 91.031 kb on - strand, within BBR_RS10575 at 91.047 kb on + strand, within BBR_RS10575 at 91.050 kb on - strand, within BBR_RS10575 at 91.050 kb on - strand, within BBR_RS10575 at 91.091 kb on - strand, within BBR_RS10575 at 91.106 kb on + strand, within BBR_RS10575 at 91.109 kb on + strand, within BBR_RS10575 at 91.109 kb on + strand, within BBR_RS10575 at 91.109 kb on + strand, within BBR_RS10575 at 91.109 kb on + strand, within BBR_RS10575 at 91.116 kb on - strand, within BBR_RS10575 at 91.117 kb on - strand, within BBR_RS10575 at 91.117 kb on - strand, within BBR_RS10575 at 91.117 kb on - strand, within BBR_RS10575 at 91.130 kb on - strand at 91.138 kb on - strand at 91.138 kb on - strand at 91.138 kb on - strand at 91.147 kb on + strand at 91.172 kb on + strand at 91.178 kb on + strand at 91.200 kb on + strand at 91.211 kb on + strand at 91.211 kb on + strand at 91.225 kb on + strand at 91.244 kb on + strand at 91.249 kb on - strand at 91.252 kb on - strand at 91.262 kb on - strand at 91.330 kb on - strand at 91.365 kb on - strand at 91.398 kb on + strand at 91.422 kb on + strand at 91.482 kb on - strand at 91.489 kb on + strand at 91.489 kb on + strand at 91.507 kb on - strand at 91.541 kb on + strand at 91.541 kb on + strand at 91.549 kb on - strand at 91.559 kb on + strand at 91.561 kb on - strand at 91.561 kb on - strand at 91.602 kb on + strand at 91.671 kb on + strand at 91.692 kb on + strand at 91.692 kb on + strand at 91.700 kb on - strand at 91.708 kb on + strand at 91.731 kb on - strand at 91.831 kb on + strand, within BBR_RS10580 at 92.028 kb on - strand, within BBR_RS10580 at 92.028 kb on - strand, within BBR_RS10580 at 92.052 kb on - strand, within BBR_RS10580 at 92.142 kb on - strand at 92.151 kb on - strand at 92.169 kb on - strand at 92.174 kb on - strand at 92.184 kb on + strand at 92.192 kb on - strand at 92.198 kb on + strand at 92.198 kb on + strand at 92.198 kb on + strand at 92.206 kb on - strand at 92.206 kb on - strand at 92.206 kb on - strand at 92.215 kb on + strand at 92.215 kb on + strand at 92.223 kb on - strand at 92.234 kb on + strand at 92.234 kb on + strand at 92.242 kb on - strand at 92.249 kb on - strand at 92.290 kb on + strand at 92.290 kb on + strand at 92.290 kb on + strand at 92.290 kb on + strand at 92.290 kb on + strand at 92.298 kb on - strand at 92.298 kb on - strand at 92.298 kb on - strand at 92.314 kb on + strand at 92.314 kb on + strand at 92.316 kb on + strand at 92.322 kb on - strand at 92.334 kb on + strand at 93.001 kb on - strand at 93.027 kb on - strand at 93.029 kb on - strand at 93.031 kb on - strand at 93.033 kb on + strand at 93.033 kb on + strand at 93.033 kb on + strand at 93.033 kb on + strand at 93.033 kb on + strand at 93.033 kb on + strand at 93.035 kb on + strand at 93.041 kb on - strand at 93.041 kb on - strand at 93.041 kb on - strand at 93.041 kb on - strand at 93.041 kb on - strand at 93.041 kb on - strand at 93.045 kb on + strand at 93.048 kb on - strand at 93.048 kb on - strand at 93.053 kb on + strand at 93.074 kb on + strand at 93.074 kb on + strand at 93.074 kb on + strand at 93.082 kb on - strand at 93.108 kb on - strand at 93.109 kb on - strand at 93.109 kb on - strand at 93.113 kb on + strand at 93.134 kb on - strand at 93.136 kb on + strand at 93.142 kb on + strand at 93.146 kb on + strand at 93.146 kb on + strand at 93.147 kb on + strand at 93.147 kb on + strand at 93.155 kb on - strand at 93.171 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Streptomycin 0.3 mM remove 90,729 - BBR_RS10575 0.37 -1.0 90,729 - BBR_RS10575 0.37 +1.4 90,729 - BBR_RS10575 0.37 -1.7 90,729 - BBR_RS10575 0.37 +0.5 90,729 - BBR_RS10575 0.37 -0.3 90,729 - BBR_RS10575 0.37 +0.4 90,732 + BBR_RS10575 0.37 -1.7 90,732 + BBR_RS10575 0.37 -1.0 90,737 + BBR_RS10575 0.38 -1.0 90,740 - BBR_RS10575 0.38 -0.9 90,756 - BBR_RS10575 0.41 -0.2 90,768 + BBR_RS10575 0.42 -1.8 90,782 - BBR_RS10575 0.44 -0.0 90,786 + BBR_RS10575 0.45 -1.7 90,808 + BBR_RS10575 0.48 -1.4 90,810 + BBR_RS10575 0.48 +0.5 90,810 + BBR_RS10575 0.48 +0.1 90,810 + BBR_RS10575 0.48 -0.7 90,816 - BBR_RS10575 0.49 -0.4 90,816 - BBR_RS10575 0.49 -0.6 90,816 - BBR_RS10575 0.49 -0.2 90,816 - BBR_RS10575 0.49 -0.1 90,816 - BBR_RS10575 0.49 -2.3 90,817 + BBR_RS10575 0.49 -1.4 90,817 + BBR_RS10575 0.49 -0.4 90,817 + BBR_RS10575 0.49 -0.7 90,817 + BBR_RS10575 0.49 -1.2 90,818 - BBR_RS10575 0.49 -0.3 90,824 - BBR_RS10575 0.50 -0.5 90,825 - BBR_RS10575 0.50 -0.2 90,827 + BBR_RS10575 0.50 +0.0 90,827 + BBR_RS10575 0.50 -0.1 90,827 + BBR_RS10575 0.50 -1.2 90,827 + BBR_RS10575 0.50 -0.8 90,835 - BBR_RS10575 0.51 -1.6 90,835 - BBR_RS10575 0.51 -0.3 90,835 - BBR_RS10575 0.51 -1.4 90,842 + BBR_RS10575 0.52 -0.7 90,850 - BBR_RS10575 0.53 -1.2 90,871 + BBR_RS10575 0.56 -1.5 90,881 - BBR_RS10575 0.57 -1.6 90,886 + BBR_RS10575 0.58 +0.4 90,907 + BBR_RS10575 0.61 -1.8 90,932 + BBR_RS10575 0.64 -0.9 90,942 - BBR_RS10575 0.66 +1.0 90,950 - BBR_RS10575 0.67 -0.3 90,955 - BBR_RS10575 0.67 -1.3 90,960 - BBR_RS10575 0.68 +0.1 90,962 + BBR_RS10575 0.68 -0.4 90,962 + BBR_RS10575 0.68 +1.6 90,977 - BBR_RS10575 0.70 -1.2 90,979 + BBR_RS10575 0.71 -1.9 90,979 + BBR_RS10575 0.71 +0.1 90,981 + BBR_RS10575 0.71 -0.1 91,008 - BBR_RS10575 0.75 -1.9 91,008 - BBR_RS10575 0.75 -1.2 91,023 - BBR_RS10575 0.77 -1.1 91,028 + BBR_RS10575 0.77 -0.4 91,031 - BBR_RS10575 0.78 -1.5 91,047 + BBR_RS10575 0.80 +0.1 91,050 - BBR_RS10575 0.80 +0.7 91,050 - BBR_RS10575 0.80 +0.0 91,091 - BBR_RS10575 0.86 -0.1 91,106 + BBR_RS10575 0.88 -2.0 91,109 + BBR_RS10575 0.88 +1.0 91,109 + BBR_RS10575 0.88 -0.2 91,109 + BBR_RS10575 0.88 -2.2 91,109 + BBR_RS10575 0.88 -1.6 91,116 - BBR_RS10575 0.89 -1.0 91,117 - BBR_RS10575 0.89 -0.5 91,117 - BBR_RS10575 0.89 -0.8 91,117 - BBR_RS10575 0.89 +1.1 91,130 - -1.3 91,138 - -1.8 91,138 - -1.3 91,138 - -2.1 91,147 + +0.7 91,172 + -0.5 91,178 + -1.9 91,200 + +0.2 91,211 + +1.9 91,211 + +0.1 91,225 + +0.2 91,244 + +1.7 91,249 - +0.1 91,252 - +0.1 91,262 - -2.0 91,330 - +0.8 91,365 - -2.0 91,398 + +0.1 91,422 + +0.2 91,482 - +1.6 91,489 + +1.7 91,489 + +0.9 91,507 - +1.3 91,541 + -1.9 91,541 + +2.0 91,549 - -2.4 91,559 + -1.7 91,561 - -1.2 91,561 - +1.8 91,602 + +1.2 91,671 + -0.9 91,692 + -2.6 91,692 + +0.9 91,700 - -1.8 91,708 + +0.6 91,731 - +0.5 91,831 + BBR_RS10580 0.23 +1.6 92,028 - BBR_RS10580 0.66 +0.4 92,028 - BBR_RS10580 0.66 -1.5 92,052 - BBR_RS10580 0.71 -3.2 92,142 - -2.1 92,151 - +0.4 92,169 - +0.6 92,174 - +0.7 92,184 + -0.7 92,192 - -1.8 92,198 + -0.6 92,198 + -2.1 92,198 + -0.7 92,206 - -1.7 92,206 - +0.1 92,206 - +1.1 92,215 + -0.5 92,215 + -1.3 92,223 - -2.1 92,234 + -0.1 92,234 + +0.2 92,242 - +0.5 92,249 - -0.7 92,290 + -0.9 92,290 + -2.8 92,290 + +0.7 92,290 + -2.1 92,290 + -3.6 92,298 - -0.5 92,298 - -2.0 92,298 - -2.0 92,314 + -1.2 92,314 + -0.8 92,316 + -0.2 92,322 - -0.6 92,334 + -1.1 93,001 - -2.6 93,027 - -0.2 93,029 - +1.3 93,031 - +0.7 93,033 + +0.4 93,033 + +0.2 93,033 + -1.5 93,033 + +1.7 93,033 + -0.1 93,033 + +1.5 93,035 + +4.6 93,041 - +0.5 93,041 - +1.0 93,041 - -0.4 93,041 - -0.1 93,041 - -0.3 93,041 - +0.1 93,045 + +1.9 93,048 - +0.5 93,048 - -3.0 93,053 + -0.5 93,074 + -0.9 93,074 + +0.1 93,074 + +1.5 93,082 - +0.6 93,108 - +0.9 93,109 - -0.9 93,109 - -0.5 93,113 + -1.0 93,134 - +0.7 93,136 + +0.9 93,142 + -0.1 93,146 + -0.5 93,146 + +0.2 93,147 + +1.3 93,147 + +1.6 93,155 - +1.4 93,171 - +0.1
Or see this region's nucleotide sequence