Experiment: RCH2_defined_glucose_5mM with Pyridoxal 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE53_000596 and ABIE53_000597 are separated by 3 nucleotides ABIE53_000597 and ABIE53_000598 are separated by 16 nucleotides ABIE53_000598 and ABIE53_000599 are separated by 149 nucleotides
ABIE53_000596: ABIE53_000596 - ABC-type Fe3+-hydroxamate transport system substrate-binding protein, at 654,700 to 655,527
_000596
ABIE53_000597: ABIE53_000597 - short-subunit dehydrogenase, at 655,531 to 656,304
_000597
ABIE53_000598: ABIE53_000598 - thiol:disulfide interchange protein DsbA, at 656,321 to 657,013
_000598
ABIE53_000599: ABIE53_000599 - cell division protein FtsN, at 657,163 to 657,990
_000599
Position (kb) 655
656
657 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 654.546 kb on + strand at 654.615 kb on + strand at 654.615 kb on + strand at 654.616 kb on - strand at 654.645 kb on + strand at 654.645 kb on + strand at 654.646 kb on - strand at 654.646 kb on - strand at 654.646 kb on - strand at 654.646 kb on - strand at 654.660 kb on + strand at 654.670 kb on + strand at 654.671 kb on - strand at 654.729 kb on - strand at 654.729 kb on - strand at 654.729 kb on - strand at 654.758 kb on + strand at 654.854 kb on + strand, within ABIE53_000596 at 654.855 kb on - strand, within ABIE53_000596 at 654.855 kb on - strand, within ABIE53_000596 at 654.855 kb on - strand, within ABIE53_000596 at 655.013 kb on + strand, within ABIE53_000596 at 655.013 kb on + strand, within ABIE53_000596 at 655.013 kb on + strand, within ABIE53_000596 at 655.013 kb on + strand, within ABIE53_000596 at 655.013 kb on + strand, within ABIE53_000596 at 655.013 kb on + strand, within ABIE53_000596 at 655.013 kb on + strand, within ABIE53_000596 at 655.013 kb on + strand, within ABIE53_000596 at 655.013 kb on + strand, within ABIE53_000596 at 655.013 kb on + strand, within ABIE53_000596 at 655.013 kb on + strand, within ABIE53_000596 at 655.013 kb on + strand, within ABIE53_000596 at 655.013 kb on + strand, within ABIE53_000596 at 655.013 kb on + strand, within ABIE53_000596 at 655.013 kb on + strand, within ABIE53_000596 at 655.013 kb on + strand, within ABIE53_000596 at 655.013 kb on + strand, within ABIE53_000596 at 655.014 kb on - strand, within ABIE53_000596 at 655.014 kb on - strand, within ABIE53_000596 at 655.014 kb on - strand, within ABIE53_000596 at 655.014 kb on - strand, within ABIE53_000596 at 655.014 kb on - strand, within ABIE53_000596 at 655.014 kb on - strand, within ABIE53_000596 at 655.014 kb on - strand, within ABIE53_000596 at 655.014 kb on - strand, within ABIE53_000596 at 655.014 kb on - strand, within ABIE53_000596 at 655.014 kb on - strand, within ABIE53_000596 at 655.014 kb on - strand, within ABIE53_000596 at 655.014 kb on - strand, within ABIE53_000596 at 655.014 kb on - strand, within ABIE53_000596 at 655.014 kb on - strand, within ABIE53_000596 at 655.014 kb on - strand, within ABIE53_000596 at 655.058 kb on + strand, within ABIE53_000596 at 655.058 kb on + strand, within ABIE53_000596 at 655.058 kb on + strand, within ABIE53_000596 at 655.058 kb on + strand, within ABIE53_000596 at 655.058 kb on + strand, within ABIE53_000596 at 655.058 kb on + strand, within ABIE53_000596 at 655.059 kb on - strand, within ABIE53_000596 at 655.059 kb on - strand, within ABIE53_000596 at 655.059 kb on - strand, within ABIE53_000596 at 655.176 kb on - strand, within ABIE53_000596 at 655.176 kb on - strand, within ABIE53_000596 at 655.176 kb on - strand, within ABIE53_000596 at 655.275 kb on + strand, within ABIE53_000596 at 655.293 kb on + strand, within ABIE53_000596 at 655.293 kb on + strand, within ABIE53_000596 at 655.293 kb on + strand, within ABIE53_000596 at 655.392 kb on + strand, within ABIE53_000596 at 655.414 kb on + strand, within ABIE53_000596 at 655.533 kb on - strand at 655.583 kb on + strand at 655.583 kb on + strand at 655.583 kb on + strand at 655.724 kb on + strand, within ABIE53_000597 at 655.724 kb on + strand, within ABIE53_000597 at 655.724 kb on + strand, within ABIE53_000597 at 655.725 kb on - strand, within ABIE53_000597 at 655.725 kb on - strand, within ABIE53_000597 at 655.755 kb on + strand, within ABIE53_000597 at 655.755 kb on + strand, within ABIE53_000597 at 655.755 kb on + strand, within ABIE53_000597 at 655.756 kb on - strand, within ABIE53_000597 at 655.756 kb on - strand, within ABIE53_000597 at 655.757 kb on + strand, within ABIE53_000597 at 655.757 kb on + strand, within ABIE53_000597 at 655.758 kb on - strand, within ABIE53_000597 at 655.823 kb on + strand, within ABIE53_000597 at 655.823 kb on + strand, within ABIE53_000597 at 655.823 kb on + strand, within ABIE53_000597 at 655.824 kb on - strand, within ABIE53_000597 at 655.824 kb on - strand, within ABIE53_000597 at 655.824 kb on - strand, within ABIE53_000597 at 655.824 kb on - strand, within ABIE53_000597 at 655.851 kb on + strand, within ABIE53_000597 at 655.852 kb on - strand, within ABIE53_000597 at 655.852 kb on - strand, within ABIE53_000597 at 655.852 kb on - strand, within ABIE53_000597 at 655.852 kb on - strand, within ABIE53_000597 at 655.853 kb on + strand, within ABIE53_000597 at 655.853 kb on + strand, within ABIE53_000597 at 655.853 kb on + strand, within ABIE53_000597 at 655.853 kb on + strand, within ABIE53_000597 at 655.853 kb on + strand, within ABIE53_000597 at 655.973 kb on + strand, within ABIE53_000597 at 655.973 kb on + strand, within ABIE53_000597 at 655.973 kb on + strand, within ABIE53_000597 at 655.973 kb on + strand, within ABIE53_000597 at 655.973 kb on + strand, within ABIE53_000597 at 655.973 kb on + strand, within ABIE53_000597 at 655.974 kb on - strand, within ABIE53_000597 at 655.974 kb on - strand, within ABIE53_000597 at 655.974 kb on - strand, within ABIE53_000597 at 655.974 kb on - strand, within ABIE53_000597 at 655.974 kb on - strand, within ABIE53_000597 at 655.974 kb on - strand, within ABIE53_000597 at 656.038 kb on + strand, within ABIE53_000597 at 656.038 kb on + strand, within ABIE53_000597 at 656.039 kb on - strand, within ABIE53_000597 at 656.039 kb on - strand, within ABIE53_000597 at 656.136 kb on + strand, within ABIE53_000597 at 656.136 kb on + strand, within ABIE53_000597 at 656.138 kb on + strand, within ABIE53_000597 at 656.138 kb on + strand, within ABIE53_000597 at 656.138 kb on + strand, within ABIE53_000597 at 656.138 kb on + strand, within ABIE53_000597 at 656.138 kb on + strand, within ABIE53_000597 at 656.138 kb on + strand, within ABIE53_000597 at 656.139 kb on - strand, within ABIE53_000597 at 656.139 kb on - strand, within ABIE53_000597 at 656.139 kb on - strand, within ABIE53_000597 at 656.322 kb on + strand at 656.322 kb on + strand at 656.322 kb on + strand at 656.322 kb on + strand at 656.322 kb on + strand at 656.322 kb on + strand at 656.323 kb on - strand at 656.323 kb on - strand at 656.323 kb on - strand at 656.355 kb on + strand at 656.355 kb on + strand at 656.412 kb on + strand, within ABIE53_000598 at 656.412 kb on + strand, within ABIE53_000598 at 656.413 kb on - strand, within ABIE53_000598 at 656.454 kb on + strand, within ABIE53_000598 at 656.511 kb on + strand, within ABIE53_000598 at 656.523 kb on + strand, within ABIE53_000598 at 656.523 kb on + strand, within ABIE53_000598 at 656.523 kb on + strand, within ABIE53_000598 at 656.524 kb on - strand, within ABIE53_000598 at 656.524 kb on - strand, within ABIE53_000598 at 656.524 kb on - strand, within ABIE53_000598 at 656.524 kb on - strand, within ABIE53_000598 at 656.586 kb on + strand, within ABIE53_000598 at 656.586 kb on + strand, within ABIE53_000598 at 656.587 kb on - strand, within ABIE53_000598 at 656.664 kb on + strand, within ABIE53_000598 at 656.665 kb on - strand, within ABIE53_000598 at 656.665 kb on - strand, within ABIE53_000598 at 656.665 kb on - strand, within ABIE53_000598 at 656.665 kb on - strand, within ABIE53_000598 at 656.760 kb on + strand, within ABIE53_000598 at 656.761 kb on - strand, within ABIE53_000598 at 656.793 kb on + strand, within ABIE53_000598 at 656.794 kb on - strand, within ABIE53_000598 at 656.794 kb on - strand, within ABIE53_000598 at 656.865 kb on + strand, within ABIE53_000598 at 656.865 kb on + strand, within ABIE53_000598 at 656.976 kb on + strand at 656.976 kb on + strand at 656.977 kb on - strand at 656.977 kb on - strand at 656.982 kb on + strand at 656.982 kb on + strand at 656.982 kb on + strand at 656.982 kb on + strand at 656.982 kb on + strand at 656.982 kb on + strand at 656.983 kb on - strand at 656.983 kb on - strand at 657.077 kb on + strand at 657.077 kb on + strand at 657.077 kb on + strand at 657.077 kb on + strand at 657.078 kb on - strand at 657.078 kb on - strand at 657.078 kb on - strand at 657.078 kb on - strand at 657.078 kb on - strand at 657.078 kb on - strand at 657.124 kb on + strand at 657.125 kb on - strand at 657.164 kb on + strand at 657.164 kb on + strand at 657.164 kb on + strand at 657.164 kb on + strand at 657.164 kb on + strand at 657.164 kb on + strand at 657.164 kb on + strand at 657.165 kb on - strand at 657.165 kb on - strand at 657.165 kb on - strand at 657.165 kb on - strand at 657.165 kb on - strand at 657.165 kb on - strand at 657.165 kb on - strand at 657.165 kb on - strand at 657.165 kb on - strand at 657.165 kb on - strand at 657.165 kb on - strand at 657.275 kb on + strand, within ABIE53_000599 at 657.275 kb on + strand, within ABIE53_000599 at 657.275 kb on + strand, within ABIE53_000599 at 657.275 kb on + strand, within ABIE53_000599 at 657.275 kb on + strand, within ABIE53_000599 at 657.275 kb on + strand, within ABIE53_000599 at 657.276 kb on - strand, within ABIE53_000599 at 657.276 kb on - strand, within ABIE53_000599 at 657.276 kb on - strand, within ABIE53_000599 at 657.276 kb on - strand, within ABIE53_000599
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose_5mM with Pyridoxal 0.5 mM remove 654,546 + +2.1 654,615 + +0.2 654,615 + +0.6 654,616 - +1.8 654,645 + +1.4 654,645 + -1.0 654,646 - -0.7 654,646 - -0.1 654,646 - +0.8 654,646 - -0.3 654,660 + -0.5 654,670 + +0.2 654,671 - +0.6 654,729 - -1.8 654,729 - -1.4 654,729 - -0.1 654,758 + +0.2 654,854 + ABIE53_000596 0.19 -0.5 654,855 - ABIE53_000596 0.19 -0.0 654,855 - ABIE53_000596 0.19 -0.3 654,855 - ABIE53_000596 0.19 +0.5 655,013 + ABIE53_000596 0.38 +1.1 655,013 + ABIE53_000596 0.38 -1.4 655,013 + ABIE53_000596 0.38 +0.7 655,013 + ABIE53_000596 0.38 -0.8 655,013 + ABIE53_000596 0.38 -0.6 655,013 + ABIE53_000596 0.38 -0.5 655,013 + ABIE53_000596 0.38 +1.4 655,013 + ABIE53_000596 0.38 -1.7 655,013 + ABIE53_000596 0.38 -2.4 655,013 + ABIE53_000596 0.38 +1.1 655,013 + ABIE53_000596 0.38 -0.3 655,013 + ABIE53_000596 0.38 +1.5 655,013 + ABIE53_000596 0.38 -0.4 655,013 + ABIE53_000596 0.38 -0.1 655,013 + ABIE53_000596 0.38 +1.0 655,013 + ABIE53_000596 0.38 -1.2 655,013 + ABIE53_000596 0.38 -2.7 655,014 - ABIE53_000596 0.38 -0.0 655,014 - ABIE53_000596 0.38 +0.8 655,014 - ABIE53_000596 0.38 -0.5 655,014 - ABIE53_000596 0.38 -0.6 655,014 - ABIE53_000596 0.38 +0.3 655,014 - ABIE53_000596 0.38 +0.8 655,014 - ABIE53_000596 0.38 -0.3 655,014 - ABIE53_000596 0.38 +0.5 655,014 - ABIE53_000596 0.38 -0.8 655,014 - ABIE53_000596 0.38 -1.1 655,014 - ABIE53_000596 0.38 +0.8 655,014 - ABIE53_000596 0.38 -1.0 655,014 - ABIE53_000596 0.38 +1.1 655,014 - ABIE53_000596 0.38 +1.1 655,014 - ABIE53_000596 0.38 +0.3 655,058 + ABIE53_000596 0.43 -0.3 655,058 + ABIE53_000596 0.43 -1.0 655,058 + ABIE53_000596 0.43 -0.1 655,058 + ABIE53_000596 0.43 -1.7 655,058 + ABIE53_000596 0.43 -0.1 655,058 + ABIE53_000596 0.43 -0.4 655,059 - ABIE53_000596 0.43 -0.0 655,059 - ABIE53_000596 0.43 -0.5 655,059 - ABIE53_000596 0.43 -0.1 655,176 - ABIE53_000596 0.57 +0.2 655,176 - ABIE53_000596 0.57 +0.8 655,176 - ABIE53_000596 0.57 -0.4 655,275 + ABIE53_000596 0.69 -0.4 655,293 + ABIE53_000596 0.72 -0.3 655,293 + ABIE53_000596 0.72 -1.5 655,293 + ABIE53_000596 0.72 -0.2 655,392 + ABIE53_000596 0.84 -0.8 655,414 + ABIE53_000596 0.86 +1.2 655,533 - +0.7 655,583 + -0.4 655,583 + +0.0 655,583 + +0.3 655,724 + ABIE53_000597 0.25 +0.4 655,724 + ABIE53_000597 0.25 -0.9 655,724 + ABIE53_000597 0.25 +1.8 655,725 - ABIE53_000597 0.25 -0.1 655,725 - ABIE53_000597 0.25 -0.5 655,755 + ABIE53_000597 0.29 -0.0 655,755 + ABIE53_000597 0.29 +1.0 655,755 + ABIE53_000597 0.29 -1.9 655,756 - ABIE53_000597 0.29 -0.8 655,756 - ABIE53_000597 0.29 -0.8 655,757 + ABIE53_000597 0.29 -2.6 655,757 + ABIE53_000597 0.29 -1.6 655,758 - ABIE53_000597 0.29 -1.6 655,823 + ABIE53_000597 0.38 +0.2 655,823 + ABIE53_000597 0.38 +1.8 655,823 + ABIE53_000597 0.38 +1.5 655,824 - ABIE53_000597 0.38 -2.2 655,824 - ABIE53_000597 0.38 -0.4 655,824 - ABIE53_000597 0.38 -0.3 655,824 - ABIE53_000597 0.38 +0.4 655,851 + ABIE53_000597 0.41 -1.8 655,852 - ABIE53_000597 0.41 -0.1 655,852 - ABIE53_000597 0.41 -0.5 655,852 - ABIE53_000597 0.41 -1.0 655,852 - ABIE53_000597 0.41 -0.4 655,853 + ABIE53_000597 0.42 +0.8 655,853 + ABIE53_000597 0.42 +0.3 655,853 + ABIE53_000597 0.42 +1.0 655,853 + ABIE53_000597 0.42 +1.8 655,853 + ABIE53_000597 0.42 -0.8 655,973 + ABIE53_000597 0.57 -0.1 655,973 + ABIE53_000597 0.57 -1.4 655,973 + ABIE53_000597 0.57 -0.4 655,973 + ABIE53_000597 0.57 +0.7 655,973 + ABIE53_000597 0.57 -0.2 655,973 + ABIE53_000597 0.57 -1.2 655,974 - ABIE53_000597 0.57 -0.5 655,974 - ABIE53_000597 0.57 -0.2 655,974 - ABIE53_000597 0.57 +0.8 655,974 - ABIE53_000597 0.57 -0.8 655,974 - ABIE53_000597 0.57 +0.2 655,974 - ABIE53_000597 0.57 +0.0 656,038 + ABIE53_000597 0.66 -0.6 656,038 + ABIE53_000597 0.66 -0.4 656,039 - ABIE53_000597 0.66 -0.3 656,039 - ABIE53_000597 0.66 +2.4 656,136 + ABIE53_000597 0.78 +0.2 656,136 + ABIE53_000597 0.78 +0.8 656,138 + ABIE53_000597 0.78 +0.2 656,138 + ABIE53_000597 0.78 +0.7 656,138 + ABIE53_000597 0.78 -0.2 656,138 + ABIE53_000597 0.78 -0.8 656,138 + ABIE53_000597 0.78 -0.8 656,138 + ABIE53_000597 0.78 -1.2 656,139 - ABIE53_000597 0.79 -1.4 656,139 - ABIE53_000597 0.79 +0.0 656,139 - ABIE53_000597 0.79 +0.4 656,322 + -0.2 656,322 + -0.8 656,322 + -0.1 656,322 + -1.8 656,322 + -1.7 656,322 + -0.1 656,323 - -1.0 656,323 - +1.0 656,323 - +0.6 656,355 + -0.8 656,355 + +0.2 656,412 + ABIE53_000598 0.13 -1.9 656,412 + ABIE53_000598 0.13 -1.7 656,413 - ABIE53_000598 0.13 -1.9 656,454 + ABIE53_000598 0.19 -3.0 656,511 + ABIE53_000598 0.27 -0.8 656,523 + ABIE53_000598 0.29 -1.2 656,523 + ABIE53_000598 0.29 -1.0 656,523 + ABIE53_000598 0.29 -2.1 656,524 - ABIE53_000598 0.29 -0.5 656,524 - ABIE53_000598 0.29 -1.5 656,524 - ABIE53_000598 0.29 -1.5 656,524 - ABIE53_000598 0.29 -1.4 656,586 + ABIE53_000598 0.38 -1.8 656,586 + ABIE53_000598 0.38 +0.2 656,587 - ABIE53_000598 0.38 -3.1 656,664 + ABIE53_000598 0.49 -1.4 656,665 - ABIE53_000598 0.50 -1.5 656,665 - ABIE53_000598 0.50 -3.5 656,665 - ABIE53_000598 0.50 -0.2 656,665 - ABIE53_000598 0.50 -1.2 656,760 + ABIE53_000598 0.63 +0.8 656,761 - ABIE53_000598 0.63 -1.0 656,793 + ABIE53_000598 0.68 +0.2 656,794 - ABIE53_000598 0.68 -3.9 656,794 - ABIE53_000598 0.68 +0.2 656,865 + ABIE53_000598 0.78 -2.8 656,865 + ABIE53_000598 0.78 -1.2 656,976 + -1.5 656,976 + -1.5 656,977 - -0.1 656,977 - +1.7 656,982 + +0.2 656,982 + +0.8 656,982 + -2.3 656,982 + -0.5 656,982 + -1.4 656,982 + +0.8 656,983 - -0.3 656,983 - -0.5 657,077 + -0.9 657,077 + -0.8 657,077 + -3.4 657,077 + +0.0 657,078 - -0.4 657,078 - -2.1 657,078 - -0.0 657,078 - -0.4 657,078 - -0.2 657,078 - +0.5 657,124 + -1.4 657,125 - +0.8 657,164 + -1.0 657,164 + -1.7 657,164 + -1.0 657,164 + -0.4 657,164 + -0.5 657,164 + +1.8 657,164 + -0.8 657,165 - +0.2 657,165 - +0.4 657,165 - -1.7 657,165 - -0.5 657,165 - -0.2 657,165 - -0.3 657,165 - -0.4 657,165 - -0.2 657,165 - +0.7 657,165 - +0.8 657,165 - -1.0 657,275 + ABIE53_000599 0.14 -1.4 657,275 + ABIE53_000599 0.14 -0.8 657,275 + ABIE53_000599 0.14 +0.8 657,275 + ABIE53_000599 0.14 -0.4 657,275 + ABIE53_000599 0.14 +0.2 657,275 + ABIE53_000599 0.14 -0.6 657,276 - ABIE53_000599 0.14 -1.3 657,276 - ABIE53_000599 0.14 -0.4 657,276 - ABIE53_000599 0.14 -1.5 657,276 - ABIE53_000599 0.14 +0.1
Or see this region's nucleotide sequence