Experiment: RCH2_defined_glucose_5mM with Pyridoxal 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE53_000695 and ABIE53_000696 are separated by 39 nucleotides ABIE53_000696 and ABIE53_000697 are separated by 2 nucleotides ABIE53_000697 and ABIE53_000698 are separated by 251 nucleotides ABIE53_000698 and ABIE53_000699 are separated by 26 nucleotides
ABIE53_000695: ABIE53_000695 - lipid A 3-O-deacylase, at 751,321 to 751,884
_000695
ABIE53_000696: ABIE53_000696 - ketosteroid isomerase-like protein, at 751,924 to 752,307
_000696
ABIE53_000697: ABIE53_000697 - anthranilate phosphoribosyltransferase, at 752,310 to 753,281
_000697
ABIE53_000698: ABIE53_000698 - fumarylpyruvate hydrolase, at 753,533 to 754,231
_000698
ABIE53_000699: ABIE53_000699 - maleylpyruvate isomerase, at 754,258 to 754,902
_000699
Position (kb) 752
753
754 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 751.358 kb on + strand at 751.563 kb on + strand, within ABIE53_000695 at 751.563 kb on + strand, within ABIE53_000695 at 751.563 kb on + strand, within ABIE53_000695 at 751.563 kb on + strand, within ABIE53_000695 at 751.564 kb on - strand, within ABIE53_000695 at 751.614 kb on + strand, within ABIE53_000695 at 751.614 kb on + strand, within ABIE53_000695 at 751.615 kb on - strand, within ABIE53_000695 at 751.615 kb on - strand, within ABIE53_000695 at 751.615 kb on - strand, within ABIE53_000695 at 751.615 kb on - strand, within ABIE53_000695 at 751.783 kb on + strand, within ABIE53_000695 at 751.784 kb on - strand, within ABIE53_000695 at 751.845 kb on + strand at 751.845 kb on + strand at 751.846 kb on - strand at 751.847 kb on + strand at 751.848 kb on - strand at 751.862 kb on + strand at 751.862 kb on + strand at 751.862 kb on + strand at 751.862 kb on + strand at 751.862 kb on + strand at 751.862 kb on + strand at 751.862 kb on + strand at 751.862 kb on + strand at 751.862 kb on + strand at 751.862 kb on + strand at 751.862 kb on + strand at 751.862 kb on + strand at 751.863 kb on - strand at 751.863 kb on - strand at 751.863 kb on - strand at 751.863 kb on - strand at 751.863 kb on - strand at 751.863 kb on - strand at 751.863 kb on - strand at 751.863 kb on - strand at 751.863 kb on - strand at 751.863 kb on - strand at 751.863 kb on - strand at 751.863 kb on - strand at 751.863 kb on - strand at 751.863 kb on - strand at 751.863 kb on - strand at 751.863 kb on - strand at 751.863 kb on - strand at 751.863 kb on - strand at 751.863 kb on - strand at 751.863 kb on - strand at 751.864 kb on + strand at 751.864 kb on + strand at 751.864 kb on + strand at 751.864 kb on + strand at 751.864 kb on + strand at 751.864 kb on + strand at 751.864 kb on + strand at 751.864 kb on + strand at 751.864 kb on + strand at 751.864 kb on + strand at 751.864 kb on + strand at 751.864 kb on + strand at 751.864 kb on + strand at 751.865 kb on - strand at 751.865 kb on - strand at 751.865 kb on - strand at 751.865 kb on - strand at 751.865 kb on - strand at 751.865 kb on - strand at 751.865 kb on - strand at 751.865 kb on - strand at 751.865 kb on - strand at 751.865 kb on - strand at 751.865 kb on - strand at 751.873 kb on + strand at 751.873 kb on + strand at 751.873 kb on + strand at 751.873 kb on + strand at 751.873 kb on + strand at 751.873 kb on + strand at 751.873 kb on + strand at 751.873 kb on + strand at 751.873 kb on + strand at 751.874 kb on - strand at 751.874 kb on - strand at 751.874 kb on - strand at 751.874 kb on - strand at 751.874 kb on - strand at 751.874 kb on - strand at 751.874 kb on - strand at 751.874 kb on - strand at 751.874 kb on - strand at 751.874 kb on - strand at 751.874 kb on - strand at 751.874 kb on - strand at 751.875 kb on + strand at 751.875 kb on + strand at 751.875 kb on + strand at 751.875 kb on + strand at 751.875 kb on + strand at 751.876 kb on - strand at 751.876 kb on - strand at 751.876 kb on - strand at 751.876 kb on - strand at 751.876 kb on - strand at 751.876 kb on - strand at 751.876 kb on - strand at 751.876 kb on - strand at 751.876 kb on - strand at 751.876 kb on - strand at 751.876 kb on - strand at 751.908 kb on + strand at 751.908 kb on + strand at 751.908 kb on + strand at 751.909 kb on - strand at 751.909 kb on - strand at 752.035 kb on + strand, within ABIE53_000696 at 752.035 kb on + strand, within ABIE53_000696 at 752.035 kb on + strand, within ABIE53_000696 at 752.035 kb on + strand, within ABIE53_000696 at 752.035 kb on + strand, within ABIE53_000696 at 752.035 kb on + strand, within ABIE53_000696 at 752.035 kb on + strand, within ABIE53_000696 at 752.035 kb on + strand, within ABIE53_000696 at 752.036 kb on - strand, within ABIE53_000696 at 752.036 kb on - strand, within ABIE53_000696 at 752.036 kb on - strand, within ABIE53_000696 at 752.036 kb on - strand, within ABIE53_000696 at 752.036 kb on - strand, within ABIE53_000696 at 752.036 kb on - strand, within ABIE53_000696 at 752.036 kb on - strand, within ABIE53_000696 at 752.036 kb on - strand, within ABIE53_000696 at 752.036 kb on - strand, within ABIE53_000696 at 752.036 kb on - strand, within ABIE53_000696 at 752.036 kb on - strand, within ABIE53_000696 at 752.036 kb on - strand, within ABIE53_000696 at 752.036 kb on - strand, within ABIE53_000696 at 752.036 kb on - strand, within ABIE53_000696 at 752.036 kb on - strand, within ABIE53_000696 at 752.036 kb on - strand, within ABIE53_000696 at 752.036 kb on - strand, within ABIE53_000696 at 752.036 kb on - strand, within ABIE53_000696 at 752.088 kb on + strand, within ABIE53_000696 at 752.088 kb on + strand, within ABIE53_000696 at 752.230 kb on + strand, within ABIE53_000696 at 752.230 kb on + strand, within ABIE53_000696 at 752.230 kb on + strand, within ABIE53_000696 at 752.230 kb on + strand, within ABIE53_000696 at 752.231 kb on - strand, within ABIE53_000696 at 752.231 kb on - strand, within ABIE53_000696 at 752.231 kb on - strand, within ABIE53_000696 at 752.231 kb on - strand, within ABIE53_000696 at 752.231 kb on - strand, within ABIE53_000696 at 752.231 kb on - strand, within ABIE53_000696 at 752.231 kb on - strand, within ABIE53_000696 at 752.231 kb on - strand, within ABIE53_000696 at 752.231 kb on - strand, within ABIE53_000696 at 752.244 kb on + strand, within ABIE53_000696 at 752.244 kb on + strand, within ABIE53_000696 at 752.244 kb on + strand, within ABIE53_000696 at 752.245 kb on - strand, within ABIE53_000696 at 752.311 kb on + strand at 752.311 kb on + strand at 752.311 kb on + strand at 752.311 kb on + strand at 752.311 kb on + strand at 752.312 kb on - strand at 752.312 kb on - strand at 752.312 kb on - strand at 752.440 kb on - strand, within ABIE53_000697 at 752.440 kb on - strand, within ABIE53_000697 at 752.613 kb on + strand, within ABIE53_000697 at 752.613 kb on + strand, within ABIE53_000697 at 752.613 kb on + strand, within ABIE53_000697 at 752.613 kb on + strand, within ABIE53_000697 at 752.614 kb on - strand, within ABIE53_000697 at 752.635 kb on + strand, within ABIE53_000697 at 752.650 kb on + strand, within ABIE53_000697 at 752.650 kb on + strand, within ABIE53_000697 at 752.650 kb on + strand, within ABIE53_000697 at 752.650 kb on + strand, within ABIE53_000697 at 752.650 kb on + strand, within ABIE53_000697 at 752.650 kb on + strand, within ABIE53_000697 at 752.650 kb on + strand, within ABIE53_000697 at 752.650 kb on + strand, within ABIE53_000697 at 752.650 kb on + strand, within ABIE53_000697 at 752.650 kb on + strand, within ABIE53_000697 at 752.651 kb on - strand, within ABIE53_000697 at 752.651 kb on - strand, within ABIE53_000697 at 752.651 kb on - strand, within ABIE53_000697 at 752.651 kb on - strand, within ABIE53_000697 at 752.651 kb on - strand, within ABIE53_000697 at 752.651 kb on - strand, within ABIE53_000697 at 752.651 kb on - strand, within ABIE53_000697 at 752.651 kb on - strand, within ABIE53_000697 at 752.651 kb on - strand, within ABIE53_000697 at 752.651 kb on - strand, within ABIE53_000697 at 752.651 kb on - strand, within ABIE53_000697 at 752.651 kb on - strand, within ABIE53_000697 at 752.651 kb on - strand, within ABIE53_000697 at 752.651 kb on - strand, within ABIE53_000697 at 752.651 kb on - strand, within ABIE53_000697 at 752.823 kb on - strand, within ABIE53_000697 at 752.990 kb on - strand, within ABIE53_000697 at 752.990 kb on - strand, within ABIE53_000697 at 752.990 kb on - strand, within ABIE53_000697 at 753.107 kb on - strand, within ABIE53_000697 at 753.166 kb on + strand, within ABIE53_000697 at 753.166 kb on + strand, within ABIE53_000697 at 753.166 kb on + strand, within ABIE53_000697 at 753.166 kb on + strand, within ABIE53_000697 at 753.166 kb on + strand, within ABIE53_000697 at 753.166 kb on + strand, within ABIE53_000697 at 753.167 kb on - strand, within ABIE53_000697 at 753.167 kb on - strand, within ABIE53_000697 at 753.167 kb on - strand, within ABIE53_000697 at 753.293 kb on - strand at 753.331 kb on - strand at 753.331 kb on - strand at 753.331 kb on - strand at 753.358 kb on - strand at 753.366 kb on + strand at 753.366 kb on + strand at 753.366 kb on + strand at 753.366 kb on + strand at 753.366 kb on + strand at 753.366 kb on + strand at 753.366 kb on + strand at 753.366 kb on + strand at 753.366 kb on + strand at 753.366 kb on + strand at 753.366 kb on + strand at 753.366 kb on + strand at 753.366 kb on + strand at 753.366 kb on + strand at 753.366 kb on + strand at 753.366 kb on + strand at 753.366 kb on + strand at 753.367 kb on - strand at 753.367 kb on - strand at 753.367 kb on - strand at 753.367 kb on - strand at 753.367 kb on - strand at 753.367 kb on - strand at 753.463 kb on + strand at 753.464 kb on - strand at 753.464 kb on - strand at 753.464 kb on - strand at 753.488 kb on + strand at 753.488 kb on + strand at 753.488 kb on + strand at 753.488 kb on + strand at 753.489 kb on - strand at 753.516 kb on + strand at 753.517 kb on - strand at 753.517 kb on - strand at 753.539 kb on + strand at 753.539 kb on + strand at 753.539 kb on + strand at 753.539 kb on + strand at 753.539 kb on + strand at 753.540 kb on - strand at 753.540 kb on - strand at 753.540 kb on - strand at 753.540 kb on - strand at 753.543 kb on + strand at 753.543 kb on + strand at 753.543 kb on + strand at 753.543 kb on + strand at 753.543 kb on + strand at 753.543 kb on + strand at 753.543 kb on + strand at 753.543 kb on + strand at 753.543 kb on + strand at 753.543 kb on + strand at 753.543 kb on + strand at 753.543 kb on + strand at 753.543 kb on + strand at 753.543 kb on + strand at 753.543 kb on + strand at 753.543 kb on + strand at 753.544 kb on - strand at 753.544 kb on - strand at 753.544 kb on - strand at 753.544 kb on - strand at 753.544 kb on - strand at 753.544 kb on - strand at 753.544 kb on - strand at 753.544 kb on - strand at 753.544 kb on - strand at 753.544 kb on - strand at 753.544 kb on - strand at 753.544 kb on - strand at 753.544 kb on - strand at 753.544 kb on - strand at 753.544 kb on - strand at 753.544 kb on - strand at 753.561 kb on + strand at 753.561 kb on + strand at 753.561 kb on + strand at 753.562 kb on - strand at 753.562 kb on - strand at 753.562 kb on - strand at 753.586 kb on + strand at 753.586 kb on + strand at 753.586 kb on + strand at 753.587 kb on - strand at 753.587 kb on - strand at 753.587 kb on - strand at 753.587 kb on - strand at 753.587 kb on - strand at 753.614 kb on + strand, within ABIE53_000698 at 753.615 kb on - strand, within ABIE53_000698 at 753.632 kb on + strand, within ABIE53_000698 at 753.632 kb on + strand, within ABIE53_000698 at 753.632 kb on + strand, within ABIE53_000698 at 753.632 kb on + strand, within ABIE53_000698 at 753.633 kb on - strand, within ABIE53_000698 at 753.716 kb on + strand, within ABIE53_000698 at 753.716 kb on + strand, within ABIE53_000698 at 753.716 kb on + strand, within ABIE53_000698 at 753.716 kb on + strand, within ABIE53_000698 at 753.716 kb on + strand, within ABIE53_000698 at 753.716 kb on + strand, within ABIE53_000698 at 753.716 kb on + strand, within ABIE53_000698 at 753.716 kb on + strand, within ABIE53_000698 at 753.716 kb on + strand, within ABIE53_000698 at 753.716 kb on + strand, within ABIE53_000698 at 753.716 kb on + strand, within ABIE53_000698 at 753.716 kb on + strand, within ABIE53_000698 at 753.716 kb on + strand, within ABIE53_000698 at 753.717 kb on - strand, within ABIE53_000698 at 753.717 kb on - strand, within ABIE53_000698 at 753.717 kb on - strand, within ABIE53_000698 at 753.717 kb on - strand, within ABIE53_000698 at 753.717 kb on - strand, within ABIE53_000698 at 753.717 kb on - strand, within ABIE53_000698 at 753.717 kb on - strand, within ABIE53_000698 at 753.717 kb on - strand, within ABIE53_000698 at 753.717 kb on - strand, within ABIE53_000698 at 753.717 kb on - strand, within ABIE53_000698 at 753.717 kb on - strand, within ABIE53_000698 at 753.717 kb on - strand, within ABIE53_000698 at 753.717 kb on - strand, within ABIE53_000698 at 753.717 kb on - strand, within ABIE53_000698 at 753.717 kb on - strand, within ABIE53_000698 at 753.717 kb on - strand, within ABIE53_000698 at 753.749 kb on + strand, within ABIE53_000698 at 753.750 kb on - strand, within ABIE53_000698 at 753.851 kb on + strand, within ABIE53_000698 at 753.851 kb on + strand, within ABIE53_000698 at 753.851 kb on + strand, within ABIE53_000698 at 753.851 kb on + strand, within ABIE53_000698 at 753.851 kb on + strand, within ABIE53_000698 at 753.852 kb on - strand, within ABIE53_000698 at 753.852 kb on - strand, within ABIE53_000698 at 753.852 kb on - strand, within ABIE53_000698 at 753.852 kb on - strand, within ABIE53_000698 at 753.852 kb on - strand, within ABIE53_000698 at 753.852 kb on - strand, within ABIE53_000698 at 753.874 kb on + strand, within ABIE53_000698 at 753.875 kb on - strand, within ABIE53_000698 at 753.875 kb on - strand, within ABIE53_000698 at 753.875 kb on - strand, within ABIE53_000698 at 754.084 kb on + strand, within ABIE53_000698 at 754.084 kb on + strand, within ABIE53_000698 at 754.084 kb on + strand, within ABIE53_000698 at 754.084 kb on + strand, within ABIE53_000698 at 754.084 kb on + strand, within ABIE53_000698 at 754.084 kb on + strand, within ABIE53_000698 at 754.085 kb on - strand, within ABIE53_000698 at 754.085 kb on - strand, within ABIE53_000698 at 754.085 kb on - strand, within ABIE53_000698 at 754.085 kb on - strand, within ABIE53_000698 at 754.085 kb on - strand, within ABIE53_000698 at 754.091 kb on + strand, within ABIE53_000698 at 754.091 kb on + strand, within ABIE53_000698 at 754.091 kb on + strand, within ABIE53_000698 at 754.091 kb on + strand, within ABIE53_000698 at 754.091 kb on + strand, within ABIE53_000698 at 754.091 kb on + strand, within ABIE53_000698 at 754.091 kb on + strand, within ABIE53_000698 at 754.091 kb on + strand, within ABIE53_000698 at 754.091 kb on + strand, within ABIE53_000698 at 754.091 kb on + strand, within ABIE53_000698 at 754.091 kb on + strand, within ABIE53_000698 at 754.092 kb on - strand, within ABIE53_000698 at 754.092 kb on - strand, within ABIE53_000698 at 754.092 kb on - strand, within ABIE53_000698 at 754.092 kb on - strand, within ABIE53_000698 at 754.092 kb on - strand, within ABIE53_000698 at 754.092 kb on - strand, within ABIE53_000698 at 754.092 kb on - strand, within ABIE53_000698 at 754.092 kb on - strand, within ABIE53_000698 at 754.092 kb on - strand, within ABIE53_000698 at 754.092 kb on - strand, within ABIE53_000698 at 754.092 kb on - strand, within ABIE53_000698 at 754.267 kb on + strand at 754.267 kb on + strand at 754.267 kb on + strand at 754.267 kb on + strand at 754.267 kb on + strand at 754.268 kb on - strand at 754.268 kb on - strand at 754.268 kb on - strand at 754.268 kb on - strand at 754.268 kb on - strand at 754.268 kb on - strand at 754.268 kb on - strand at 754.273 kb on + strand at 754.273 kb on + strand at 754.273 kb on + strand at 754.273 kb on + strand at 754.273 kb on + strand at 754.274 kb on - strand at 754.274 kb on - strand at 754.274 kb on - strand at 754.274 kb on - strand at 754.274 kb on - strand at 754.274 kb on - strand at 754.274 kb on - strand at 754.274 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose_5mM with Pyridoxal 1 mM remove 751,358 + +0.6 751,563 + ABIE53_000695 0.43 -0.1 751,563 + ABIE53_000695 0.43 -0.4 751,563 + ABIE53_000695 0.43 -0.2 751,563 + ABIE53_000695 0.43 -0.7 751,564 - ABIE53_000695 0.43 +0.1 751,614 + ABIE53_000695 0.52 -0.7 751,614 + ABIE53_000695 0.52 +1.3 751,615 - ABIE53_000695 0.52 +1.1 751,615 - ABIE53_000695 0.52 +1.5 751,615 - ABIE53_000695 0.52 +0.9 751,615 - ABIE53_000695 0.52 -1.5 751,783 + ABIE53_000695 0.82 +1.1 751,784 - ABIE53_000695 0.82 -0.3 751,845 + +0.2 751,845 + +0.1 751,846 - -0.2 751,847 + -0.0 751,848 - +0.1 751,862 + -0.1 751,862 + -0.2 751,862 + +1.7 751,862 + +0.1 751,862 + -0.5 751,862 + +2.5 751,862 + -0.6 751,862 + +1.4 751,862 + -0.5 751,862 + -0.5 751,862 + -0.1 751,862 + -0.5 751,863 - -0.2 751,863 - -1.2 751,863 - +0.2 751,863 - +0.1 751,863 - +0.7 751,863 - -0.5 751,863 - +1.1 751,863 - -1.0 751,863 - -0.5 751,863 - -0.6 751,863 - -0.8 751,863 - -0.6 751,863 - -1.7 751,863 - -0.9 751,863 - +1.7 751,863 - +0.4 751,863 - -0.6 751,863 - -0.2 751,863 - -1.2 751,863 - -1.8 751,864 + -1.6 751,864 + +0.4 751,864 + +0.7 751,864 + +0.1 751,864 + -0.7 751,864 + +1.1 751,864 + +0.5 751,864 + -0.6 751,864 + +0.5 751,864 + +0.3 751,864 + +0.4 751,864 + -0.9 751,864 + +0.5 751,865 - +0.4 751,865 - -0.6 751,865 - +0.4 751,865 - -1.3 751,865 - +1.5 751,865 - -0.9 751,865 - -1.3 751,865 - -1.9 751,865 - -1.2 751,865 - +2.1 751,865 - -0.7 751,873 + +0.3 751,873 + -0.8 751,873 + -1.1 751,873 + -0.7 751,873 + -0.1 751,873 + +1.1 751,873 + -0.1 751,873 + +0.1 751,873 + -0.1 751,874 - -0.2 751,874 - +1.6 751,874 - -2.1 751,874 - -1.3 751,874 - +4.4 751,874 - -0.2 751,874 - -0.7 751,874 - -0.9 751,874 - +0.5 751,874 - +1.0 751,874 - -2.5 751,874 - -1.1 751,875 + -0.2 751,875 + -0.7 751,875 + -0.8 751,875 + +0.4 751,875 + +0.7 751,876 - +2.1 751,876 - -0.4 751,876 - -1.8 751,876 - -0.5 751,876 - +0.2 751,876 - -2.1 751,876 - -3.0 751,876 - -0.1 751,876 - +2.1 751,876 - -0.2 751,876 - -0.6 751,908 + -0.6 751,908 + -0.1 751,908 + +0.1 751,909 - -1.2 751,909 - -1.0 752,035 + ABIE53_000696 0.29 -0.2 752,035 + ABIE53_000696 0.29 -0.6 752,035 + ABIE53_000696 0.29 -0.3 752,035 + ABIE53_000696 0.29 -2.5 752,035 + ABIE53_000696 0.29 -1.4 752,035 + ABIE53_000696 0.29 -0.8 752,035 + ABIE53_000696 0.29 -1.7 752,035 + ABIE53_000696 0.29 -0.7 752,036 - ABIE53_000696 0.29 -0.7 752,036 - ABIE53_000696 0.29 +2.1 752,036 - ABIE53_000696 0.29 +0.1 752,036 - ABIE53_000696 0.29 -1.0 752,036 - ABIE53_000696 0.29 +0.1 752,036 - ABIE53_000696 0.29 -0.1 752,036 - ABIE53_000696 0.29 +0.3 752,036 - ABIE53_000696 0.29 +0.3 752,036 - ABIE53_000696 0.29 -1.2 752,036 - ABIE53_000696 0.29 -0.5 752,036 - ABIE53_000696 0.29 -1.9 752,036 - ABIE53_000696 0.29 -0.5 752,036 - ABIE53_000696 0.29 +0.6 752,036 - ABIE53_000696 0.29 -0.5 752,036 - ABIE53_000696 0.29 -0.2 752,036 - ABIE53_000696 0.29 -1.6 752,036 - ABIE53_000696 0.29 -0.4 752,036 - ABIE53_000696 0.29 -2.3 752,088 + ABIE53_000696 0.43 +0.5 752,088 + ABIE53_000696 0.43 -1.9 752,230 + ABIE53_000696 0.80 -1.5 752,230 + ABIE53_000696 0.80 -0.8 752,230 + ABIE53_000696 0.80 -1.1 752,230 + ABIE53_000696 0.80 +0.1 752,231 - 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Or see this region's nucleotide sequence