Experiment: RCH2_defined_glucose_5mM with Pyridoxal 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE53_000455 and ABIE53_000456 are separated by 155 nucleotides ABIE53_000456 and ABIE53_000457 are separated by 332 nucleotides
ABIE53_000455: ABIE53_000455 - L-2-hydroxyglutarate oxidase LhgO, at 491,043 to 492,149
_000455
ABIE53_000456: ABIE53_000456 - hypothetical protein, at 492,305 to 492,616
_000456
ABIE53_000457: ABIE53_000457 - amidase, at 492,949 to 494,487
_000457
Position (kb) 492
493 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 491.402 kb on + strand, within ABIE53_000455 at 491.718 kb on + strand, within ABIE53_000455 at 491.718 kb on + strand, within ABIE53_000455 at 491.718 kb on + strand, within ABIE53_000455 at 491.719 kb on - strand, within ABIE53_000455 at 491.736 kb on + strand, within ABIE53_000455 at 491.736 kb on + strand, within ABIE53_000455 at 491.736 kb on + strand, within ABIE53_000455 at 491.736 kb on + strand, within ABIE53_000455 at 491.736 kb on + strand, within ABIE53_000455 at 491.736 kb on + strand, within ABIE53_000455 at 491.737 kb on - strand, within ABIE53_000455 at 491.737 kb on - strand, within ABIE53_000455 at 491.737 kb on - strand, within ABIE53_000455 at 491.737 kb on - strand, within ABIE53_000455 at 491.737 kb on - strand, within ABIE53_000455 at 491.737 kb on - strand, within ABIE53_000455 at 491.737 kb on - strand, within ABIE53_000455 at 491.778 kb on + strand, within ABIE53_000455 at 491.779 kb on - strand, within ABIE53_000455 at 491.779 kb on - strand, within ABIE53_000455 at 491.779 kb on - strand, within ABIE53_000455 at 491.880 kb on + strand, within ABIE53_000455 at 491.880 kb on + strand, within ABIE53_000455 at 491.880 kb on + strand, within ABIE53_000455 at 491.880 kb on + strand, within ABIE53_000455 at 491.880 kb on + strand, within ABIE53_000455 at 491.880 kb on + strand, within ABIE53_000455 at 491.880 kb on + strand, within ABIE53_000455 at 491.880 kb on + strand, within ABIE53_000455 at 491.881 kb on - strand, within ABIE53_000455 at 491.881 kb on - strand, within ABIE53_000455 at 491.881 kb on - strand, within ABIE53_000455 at 491.881 kb on - strand, within ABIE53_000455 at 491.881 kb on - strand, within ABIE53_000455 at 491.881 kb on - strand, within ABIE53_000455 at 491.913 kb on + strand, within ABIE53_000455 at 491.913 kb on + strand, within ABIE53_000455 at 491.913 kb on + strand, within ABIE53_000455 at 491.914 kb on - strand, within ABIE53_000455 at 491.931 kb on + strand, within ABIE53_000455 at 491.932 kb on - strand, within ABIE53_000455 at 491.932 kb on - strand, within ABIE53_000455 at 491.970 kb on + strand, within ABIE53_000455 at 491.970 kb on + strand, within ABIE53_000455 at 492.201 kb on + strand at 492.201 kb on + strand at 492.201 kb on + strand at 492.201 kb on + strand at 492.201 kb on + strand at 492.202 kb on - strand at 492.202 kb on - strand at 492.202 kb on - strand at 492.202 kb on - strand at 492.202 kb on - strand at 492.202 kb on - strand at 492.202 kb on - strand at 492.202 kb on - strand at 492.202 kb on - strand at 492.207 kb on + strand at 492.207 kb on + strand at 492.208 kb on - strand at 492.208 kb on - strand at 492.243 kb on + strand at 492.243 kb on + strand at 492.243 kb on + strand at 492.244 kb on - strand at 492.244 kb on - strand at 492.285 kb on + strand at 492.285 kb on + strand at 492.285 kb on + strand at 492.286 kb on - strand at 492.286 kb on - strand at 492.286 kb on - strand at 492.286 kb on - strand at 492.286 kb on - strand at 492.287 kb on + strand at 492.287 kb on + strand at 492.287 kb on + strand at 492.287 kb on + strand at 492.287 kb on + strand at 492.287 kb on + strand at 492.287 kb on + strand at 492.287 kb on + strand at 492.287 kb on + strand at 492.288 kb on - strand at 492.288 kb on - strand at 492.288 kb on - strand at 492.288 kb on - strand at 492.288 kb on - strand at 492.288 kb on - strand at 492.288 kb on - strand at 492.288 kb on - strand at 492.288 kb on - strand at 492.340 kb on + strand, within ABIE53_000456 at 492.435 kb on - strand, within ABIE53_000456 at 492.435 kb on - strand, within ABIE53_000456 at 492.435 kb on - strand, within ABIE53_000456 at 492.479 kb on + strand, within ABIE53_000456 at 492.479 kb on + strand, within ABIE53_000456 at 492.479 kb on + strand, within ABIE53_000456 at 492.479 kb on + strand, within ABIE53_000456 at 492.479 kb on + strand, within ABIE53_000456 at 492.479 kb on + strand, within ABIE53_000456 at 492.479 kb on + strand, within ABIE53_000456 at 492.479 kb on + strand, within ABIE53_000456 at 492.479 kb on + strand, within ABIE53_000456 at 492.479 kb on + strand, within ABIE53_000456 at 492.480 kb on - strand, within ABIE53_000456 at 492.480 kb on - strand, within ABIE53_000456 at 492.480 kb on - strand, within ABIE53_000456 at 492.480 kb on - strand, within ABIE53_000456 at 492.480 kb on - strand, within ABIE53_000456 at 492.480 kb on - strand, within ABIE53_000456 at 492.480 kb on - strand, within ABIE53_000456 at 492.480 kb on - strand, within ABIE53_000456 at 492.480 kb on - strand, within ABIE53_000456 at 492.515 kb on + strand, within ABIE53_000456 at 492.515 kb on + strand, within ABIE53_000456 at 492.515 kb on + strand, within ABIE53_000456 at 492.515 kb on + strand, within ABIE53_000456 at 492.515 kb on + strand, within ABIE53_000456 at 492.515 kb on + strand, within ABIE53_000456 at 492.515 kb on + strand, within ABIE53_000456 at 492.515 kb on + strand, within ABIE53_000456 at 492.515 kb on + strand, within ABIE53_000456 at 492.516 kb on - strand, within ABIE53_000456 at 492.516 kb on - strand, within ABIE53_000456 at 492.516 kb on - strand, within ABIE53_000456 at 492.516 kb on - strand, within ABIE53_000456 at 492.540 kb on - strand, within ABIE53_000456 at 492.599 kb on + strand at 492.599 kb on + strand at 492.599 kb on + strand at 492.599 kb on + strand at 492.599 kb on + strand at 492.599 kb on + strand at 492.599 kb on + strand at 492.599 kb on + strand at 492.599 kb on + strand at 492.599 kb on + strand at 492.599 kb on + strand at 492.600 kb on - strand at 492.600 kb on - strand at 492.600 kb on - strand at 492.600 kb on - strand at 492.600 kb on - strand at 492.601 kb on + strand at 492.601 kb on + strand at 492.601 kb on + strand at 492.601 kb on + strand at 492.601 kb on + strand at 492.602 kb on - strand at 492.602 kb on - strand at 492.602 kb on - strand at 492.666 kb on + strand at 492.666 kb on + strand at 492.666 kb on + strand at 492.666 kb on + strand at 492.666 kb on + strand at 492.666 kb on + strand at 492.666 kb on + strand at 492.666 kb on + strand at 492.666 kb on + strand at 492.666 kb on + strand at 492.667 kb on - strand at 492.667 kb on - strand at 492.667 kb on - strand at 492.667 kb on - strand at 492.731 kb on + strand at 492.731 kb on + strand at 492.731 kb on + strand at 492.731 kb on + strand at 492.731 kb on + strand at 492.732 kb on - strand at 492.732 kb on - strand at 492.742 kb on + strand at 492.742 kb on + strand at 492.743 kb on - strand at 492.743 kb on - strand at 492.798 kb on + strand at 492.798 kb on + strand at 492.832 kb on + strand at 492.832 kb on + strand at 492.832 kb on + strand at 492.832 kb on + strand at 492.832 kb on + strand at 492.832 kb on + strand at 492.832 kb on + strand at 492.832 kb on + strand at 492.832 kb on + strand at 492.832 kb on + strand at 492.833 kb on - strand at 492.833 kb on - strand at 492.833 kb on - strand at 492.833 kb on - strand at 492.833 kb on - strand at 492.833 kb on - strand at 492.833 kb on - strand at 492.833 kb on - strand at 492.833 kb on - strand at 492.833 kb on - strand at 492.835 kb on + strand at 492.835 kb on + strand at 492.835 kb on + strand at 492.835 kb on + strand at 492.835 kb on + strand at 492.835 kb on + strand at 492.835 kb on + strand at 492.835 kb on + strand at 492.836 kb on - strand at 492.836 kb on - strand at 492.836 kb on - strand at 492.836 kb on - strand at 492.836 kb on - strand at 492.836 kb on - strand at 492.836 kb on - strand at 492.836 kb on - strand at 492.836 kb on - strand at 492.836 kb on - strand at 492.836 kb on - strand at 492.950 kb on + strand at 492.950 kb on + strand at 492.951 kb on - strand at 493.316 kb on + strand, within ABIE53_000457
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose_5mM with Pyridoxal 0.5 mM remove 491,402 + ABIE53_000455 0.32 -0.8 491,718 + ABIE53_000455 0.61 -0.0 491,718 + ABIE53_000455 0.61 +0.7 491,718 + ABIE53_000455 0.61 -0.6 491,719 - ABIE53_000455 0.61 +0.7 491,736 + ABIE53_000455 0.63 -1.7 491,736 + ABIE53_000455 0.63 +0.3 491,736 + ABIE53_000455 0.63 -1.3 491,736 + ABIE53_000455 0.63 +1.3 491,736 + ABIE53_000455 0.63 -0.6 491,736 + ABIE53_000455 0.63 +0.9 491,737 - ABIE53_000455 0.63 -0.1 491,737 - ABIE53_000455 0.63 +0.9 491,737 - ABIE53_000455 0.63 +0.0 491,737 - ABIE53_000455 0.63 +0.3 491,737 - ABIE53_000455 0.63 +0.4 491,737 - ABIE53_000455 0.63 -0.3 491,737 - ABIE53_000455 0.63 -0.5 491,778 + ABIE53_000455 0.66 -1.6 491,779 - ABIE53_000455 0.66 -0.8 491,779 - ABIE53_000455 0.66 +0.7 491,779 - ABIE53_000455 0.66 -0.3 491,880 + ABIE53_000455 0.76 -1.0 491,880 + ABIE53_000455 0.76 +0.7 491,880 + ABIE53_000455 0.76 -0.6 491,880 + ABIE53_000455 0.76 -0.4 491,880 + ABIE53_000455 0.76 -1.0 491,880 + ABIE53_000455 0.76 -0.3 491,880 + ABIE53_000455 0.76 -0.1 491,880 + ABIE53_000455 0.76 +1.7 491,881 - ABIE53_000455 0.76 +0.7 491,881 - ABIE53_000455 0.76 +1.2 491,881 - ABIE53_000455 0.76 -0.3 491,881 - ABIE53_000455 0.76 -2.4 491,881 - ABIE53_000455 0.76 +0.1 491,881 - ABIE53_000455 0.76 -0.0 491,913 + ABIE53_000455 0.79 +0.7 491,913 + ABIE53_000455 0.79 +0.0 491,913 + ABIE53_000455 0.79 +0.2 491,914 - ABIE53_000455 0.79 -0.6 491,931 + ABIE53_000455 0.80 -0.3 491,932 - ABIE53_000455 0.80 +0.0 491,932 - ABIE53_000455 0.80 +0.5 491,970 + ABIE53_000455 0.84 +0.4 491,970 + ABIE53_000455 0.84 -0.9 492,201 + -0.4 492,201 + -0.1 492,201 + -0.3 492,201 + +1.7 492,201 + -0.5 492,202 - -0.3 492,202 - -0.6 492,202 - -0.6 492,202 - +0.4 492,202 - +1.3 492,202 - -1.8 492,202 - -0.6 492,202 - +2.3 492,202 - +1.7 492,207 + +0.1 492,207 + +0.5 492,208 - +0.2 492,208 - -0.6 492,243 + +0.2 492,243 + -0.3 492,243 + -0.3 492,244 - -0.2 492,244 - -1.1 492,285 + -1.7 492,285 + -0.3 492,285 + -0.7 492,286 - +0.3 492,286 - -1.1 492,286 - +0.7 492,286 - -0.5 492,286 - -0.7 492,287 + -0.3 492,287 + +1.7 492,287 + -0.3 492,287 + -0.8 492,287 + -0.2 492,287 + -1.1 492,287 + -0.6 492,287 + +0.9 492,287 + +0.4 492,288 - -1.3 492,288 - -0.3 492,288 - -0.1 492,288 - -0.6 492,288 - -0.2 492,288 - -0.8 492,288 - -0.6 492,288 - -1.8 492,288 - +1.9 492,340 + ABIE53_000456 0.11 +0.1 492,435 - ABIE53_000456 0.42 -1.4 492,435 - ABIE53_000456 0.42 -0.9 492,435 - ABIE53_000456 0.42 -0.5 492,479 + ABIE53_000456 0.56 +0.2 492,479 + ABIE53_000456 0.56 -0.6 492,479 + ABIE53_000456 0.56 -0.6 492,479 + ABIE53_000456 0.56 +0.0 492,479 + ABIE53_000456 0.56 +1.2 492,479 + ABIE53_000456 0.56 -0.3 492,479 + ABIE53_000456 0.56 -0.0 492,479 + ABIE53_000456 0.56 +1.5 492,479 + ABIE53_000456 0.56 -0.3 492,479 + ABIE53_000456 0.56 -0.7 492,480 - ABIE53_000456 0.56 -0.7 492,480 - ABIE53_000456 0.56 -3.7 492,480 - ABIE53_000456 0.56 +0.1 492,480 - ABIE53_000456 0.56 -0.7 492,480 - ABIE53_000456 0.56 -0.4 492,480 - ABIE53_000456 0.56 -3.6 492,480 - ABIE53_000456 0.56 -0.3 492,480 - ABIE53_000456 0.56 -1.8 492,480 - ABIE53_000456 0.56 -0.8 492,515 + ABIE53_000456 0.67 -0.6 492,515 + ABIE53_000456 0.67 -0.5 492,515 + ABIE53_000456 0.67 -1.8 492,515 + ABIE53_000456 0.67 -1.0 492,515 + ABIE53_000456 0.67 +1.2 492,515 + ABIE53_000456 0.67 -0.3 492,515 + ABIE53_000456 0.67 -0.8 492,515 + ABIE53_000456 0.67 -1.3 492,515 + ABIE53_000456 0.67 +2.2 492,516 - ABIE53_000456 0.68 -0.1 492,516 - ABIE53_000456 0.68 +0.2 492,516 - ABIE53_000456 0.68 +0.7 492,516 - ABIE53_000456 0.68 +0.2 492,540 - ABIE53_000456 0.75 -0.4 492,599 + -0.6 492,599 + +0.5 492,599 + +1.0 492,599 + -0.6 492,599 + -0.4 492,599 + -0.3 492,599 + -0.3 492,599 + +0.2 492,599 + -1.1 492,599 + +0.2 492,599 + -0.4 492,600 - +0.2 492,600 - +0.6 492,600 - +0.2 492,600 - -0.6 492,600 - +0.7 492,601 + -0.9 492,601 + -0.4 492,601 + -0.9 492,601 + -0.9 492,601 + +0.7 492,602 - -1.7 492,602 - -1.4 492,602 - -0.5 492,666 + +0.7 492,666 + -0.4 492,666 + -0.8 492,666 + -0.3 492,666 + -0.6 492,666 + -0.6 492,666 + -0.3 492,666 + +0.6 492,666 + -0.5 492,666 + +0.1 492,667 - -0.8 492,667 - -0.3 492,667 - +0.3 492,667 - +0.4 492,731 + -0.6 492,731 + +0.3 492,731 + +0.7 492,731 + -0.2 492,731 + +0.7 492,732 - -1.1 492,732 - +0.5 492,742 + +0.8 492,742 + -1.8 492,743 - -0.4 492,743 - +0.2 492,798 + -0.8 492,798 + -0.0 492,832 + -0.4 492,832 + +0.5 492,832 + -1.1 492,832 + -1.4 492,832 + -0.2 492,832 + +1.9 492,832 + -0.2 492,832 + +0.2 492,832 + -0.4 492,832 + +0.3 492,833 - +0.2 492,833 - +0.6 492,833 - +0.6 492,833 - -0.0 492,833 - +0.7 492,833 - -0.0 492,833 - -0.7 492,833 - -0.3 492,833 - -0.3 492,833 - -0.6 492,835 + +0.5 492,835 + -0.3 492,835 + -0.2 492,835 + -0.3 492,835 + +1.2 492,835 + +1.7 492,835 + +0.2 492,835 + +1.0 492,836 - +0.1 492,836 - +0.1 492,836 - +0.1 492,836 - -0.3 492,836 - +2.5 492,836 - -0.4 492,836 - -1.6 492,836 - -0.2 492,836 - -1.6 492,836 - +3.3 492,836 - +0.7 492,950 + +0.9 492,950 + +1.6 492,951 - -0.3 493,316 + ABIE53_000457 0.24 -0.8
Or see this region's nucleotide sequence