Experiment: RCH2_defined_glucose_5mM with Pyridoxal 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE53_001741 and ABIE53_001742 are separated by 227 nucleotides ABIE53_001742 and ABIE53_001743 are separated by 28 nucleotides ABIE53_001743 and ABIE53_001744 are separated by 101 nucleotides
ABIE53_001741: ABIE53_001741 - pyridoxine kinase, at 1,964,625 to 1,965,491
_001741
ABIE53_001742: ABIE53_001742 - starch synthase, at 1,965,719 to 1,966,969
_001742
ABIE53_001743: ABIE53_001743 - hypothetical protein, at 1,966,998 to 1,967,165
_001743
ABIE53_001744: ABIE53_001744 - glucose-1-phosphate adenylyltransferase, at 1,967,267 to 1,968,532
_001744
Position (kb) 1965
1966
1967 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 1965.434 kb on + strand at 1965.435 kb on - strand at 1965.494 kb on + strand at 1965.495 kb on - strand at 1965.495 kb on - strand at 1965.495 kb on - strand at 1965.599 kb on + strand at 1965.599 kb on + strand at 1965.599 kb on + strand at 1965.599 kb on + strand at 1965.599 kb on + strand at 1965.599 kb on + strand at 1965.599 kb on + strand at 1965.600 kb on - strand at 1965.600 kb on - strand at 1965.600 kb on - strand at 1965.600 kb on - strand at 1965.600 kb on - strand at 1965.600 kb on - strand at 1965.600 kb on - strand at 1965.640 kb on + strand at 1965.640 kb on + strand at 1965.640 kb on + strand at 1965.640 kb on + strand at 1965.641 kb on - strand at 1965.641 kb on - strand at 1965.641 kb on - strand at 1965.641 kb on - strand at 1965.641 kb on - strand at 1965.747 kb on + strand at 1965.747 kb on + strand at 1965.748 kb on - strand at 1965.748 kb on - strand at 1965.987 kb on + strand, within ABIE53_001742 at 1965.987 kb on + strand, within ABIE53_001742 at 1965.987 kb on + strand, within ABIE53_001742 at 1966.359 kb on + strand, within ABIE53_001742 at 1966.360 kb on - strand, within ABIE53_001742 at 1966.360 kb on - strand, within ABIE53_001742 at 1966.402 kb on + strand, within ABIE53_001742 at 1966.402 kb on + strand, within ABIE53_001742 at 1966.402 kb on + strand, within ABIE53_001742 at 1966.403 kb on - strand, within ABIE53_001742 at 1966.403 kb on - strand, within ABIE53_001742 at 1966.405 kb on - strand, within ABIE53_001742 at 1966.504 kb on - strand, within ABIE53_001742 at 1966.536 kb on + strand, within ABIE53_001742 at 1966.536 kb on + strand, within ABIE53_001742 at 1966.536 kb on + strand, within ABIE53_001742 at 1966.536 kb on + strand, within ABIE53_001742 at 1966.537 kb on - strand, within ABIE53_001742 at 1966.537 kb on - strand, within ABIE53_001742 at 1966.537 kb on - strand, within ABIE53_001742 at 1966.537 kb on - strand, within ABIE53_001742 at 1966.540 kb on - strand, within ABIE53_001742 at 1966.540 kb on - strand, within ABIE53_001742 at 1966.571 kb on + strand, within ABIE53_001742 at 1966.572 kb on + strand, within ABIE53_001742 at 1966.572 kb on + strand, within ABIE53_001742 at 1966.573 kb on - strand, within ABIE53_001742 at 1966.573 kb on - strand, within ABIE53_001742 at 1966.573 kb on - strand, within ABIE53_001742 at 1966.643 kb on + strand, within ABIE53_001742 at 1966.643 kb on + strand, within ABIE53_001742 at 1966.643 kb on + strand, within ABIE53_001742 at 1966.644 kb on - strand, within ABIE53_001742 at 1966.653 kb on + strand, within ABIE53_001742 at 1966.726 kb on - strand, within ABIE53_001742 at 1966.859 kb on + strand at 1966.859 kb on + strand at 1966.859 kb on + strand at 1966.860 kb on - strand at 1966.860 kb on - strand at 1966.860 kb on - strand at 1966.878 kb on + strand at 1966.879 kb on - strand at 1966.899 kb on + strand at 1966.900 kb on - strand at 1966.923 kb on + strand at 1966.923 kb on + strand at 1966.923 kb on + strand at 1966.923 kb on + strand at 1966.924 kb on - strand at 1966.924 kb on - strand at 1966.924 kb on - strand at 1967.144 kb on - strand, within ABIE53_001743 at 1967.144 kb on - strand, within ABIE53_001743 at 1967.164 kb on - strand at 1967.200 kb on + strand at 1967.200 kb on + strand at 1967.201 kb on - strand at 1967.201 kb on - strand at 1967.201 kb on - strand at 1967.201 kb on - strand at 1967.268 kb on + strand at 1967.268 kb on + strand at 1967.268 kb on + strand at 1967.269 kb on - strand at 1967.269 kb on - strand at 1967.269 kb on - strand at 1967.269 kb on - strand at 1967.269 kb on - strand at 1967.269 kb on - strand at 1967.269 kb on - strand at 1967.324 kb on - strand at 1967.328 kb on + strand at 1967.328 kb on + strand at 1967.328 kb on + strand at 1967.328 kb on + strand at 1967.467 kb on + strand, within ABIE53_001744 at 1967.467 kb on + strand, within ABIE53_001744 at 1967.467 kb on + strand, within ABIE53_001744 at 1967.467 kb on + strand, within ABIE53_001744 at 1967.468 kb on - strand, within ABIE53_001744 at 1967.468 kb on - strand, within ABIE53_001744 at 1967.468 kb on - strand, within ABIE53_001744 at 1967.468 kb on - strand, within ABIE53_001744 at 1967.471 kb on - strand, within ABIE53_001744 at 1967.471 kb on - strand, within ABIE53_001744 at 1967.471 kb on - strand, within ABIE53_001744 at 1967.625 kb on + strand, within ABIE53_001744 at 1967.626 kb on - strand, within ABIE53_001744 at 1967.652 kb on + strand, within ABIE53_001744 at 1967.653 kb on - strand, within ABIE53_001744 at 1967.653 kb on - strand, within ABIE53_001744 at 1967.653 kb on - strand, within ABIE53_001744 at 1967.653 kb on - strand, within ABIE53_001744 at 1967.653 kb on - strand, within ABIE53_001744 at 1967.704 kb on - strand, within ABIE53_001744 at 1967.733 kb on + strand, within ABIE53_001744 at 1967.734 kb on - strand, within ABIE53_001744 at 1967.880 kb on + strand, within ABIE53_001744 at 1967.881 kb on - strand, within ABIE53_001744 at 1967.886 kb on + strand, within ABIE53_001744 at 1967.886 kb on + strand, within ABIE53_001744 at 1967.887 kb on - strand, within ABIE53_001744 at 1967.887 kb on - strand, within ABIE53_001744 at 1967.904 kb on + strand, within ABIE53_001744 at 1967.904 kb on + strand, within ABIE53_001744 at 1967.904 kb on + strand, within ABIE53_001744 at 1967.904 kb on + strand, within ABIE53_001744 at 1967.904 kb on + strand, within ABIE53_001744 at 1967.904 kb on + strand, within ABIE53_001744 at 1967.904 kb on + strand, within ABIE53_001744 at 1967.905 kb on - strand, within ABIE53_001744 at 1967.905 kb on - strand, within ABIE53_001744 at 1967.905 kb on - strand, within ABIE53_001744 at 1967.905 kb on - strand, within ABIE53_001744
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose_5mM with Pyridoxal 1 mM remove 1,965,434 + -0.4 1,965,435 - +1.0 1,965,494 + -0.3 1,965,495 - +0.3 1,965,495 - +0.6 1,965,495 - +0.1 1,965,599 + +0.1 1,965,599 + +0.1 1,965,599 + +0.1 1,965,599 + -0.4 1,965,599 + -0.4 1,965,599 + +0.3 1,965,599 + -0.4 1,965,600 - +1.0 1,965,600 - -1.7 1,965,600 - -1.0 1,965,600 - -0.4 1,965,600 - +0.1 1,965,600 - -0.6 1,965,600 - -0.2 1,965,640 + -2.7 1,965,640 + -0.1 1,965,640 + -1.0 1,965,640 + -0.3 1,965,641 - +2.4 1,965,641 - -0.0 1,965,641 - -0.0 1,965,641 - -0.0 1,965,641 - +1.8 1,965,747 + +1.1 1,965,747 + +0.8 1,965,748 - +0.1 1,965,748 - -2.3 1,965,987 + ABIE53_001742 0.21 -0.4 1,965,987 + ABIE53_001742 0.21 +0.8 1,965,987 + ABIE53_001742 0.21 +0.3 1,966,359 + ABIE53_001742 0.51 -1.4 1,966,360 - ABIE53_001742 0.51 -0.5 1,966,360 - ABIE53_001742 0.51 -0.6 1,966,402 + ABIE53_001742 0.55 -0.5 1,966,402 + ABIE53_001742 0.55 -0.2 1,966,402 + ABIE53_001742 0.55 +0.2 1,966,403 - ABIE53_001742 0.55 +0.6 1,966,403 - ABIE53_001742 0.55 -0.1 1,966,405 - ABIE53_001742 0.55 -0.0 1,966,504 - ABIE53_001742 0.63 +3.1 1,966,536 + ABIE53_001742 0.65 -1.0 1,966,536 + ABIE53_001742 0.65 -0.9 1,966,536 + ABIE53_001742 0.65 -0.8 1,966,536 + ABIE53_001742 0.65 -0.5 1,966,537 - ABIE53_001742 0.65 -0.0 1,966,537 - ABIE53_001742 0.65 -1.0 1,966,537 - ABIE53_001742 0.65 +0.1 1,966,537 - ABIE53_001742 0.65 -2.0 1,966,540 - ABIE53_001742 0.66 -0.0 1,966,540 - ABIE53_001742 0.66 -0.4 1,966,571 + ABIE53_001742 0.68 +2.1 1,966,572 + ABIE53_001742 0.68 -1.1 1,966,572 + ABIE53_001742 0.68 -0.0 1,966,573 - ABIE53_001742 0.68 +0.5 1,966,573 - ABIE53_001742 0.68 -1.9 1,966,573 - ABIE53_001742 0.68 +0.6 1,966,643 + ABIE53_001742 0.74 -0.2 1,966,643 + ABIE53_001742 0.74 -0.3 1,966,643 + ABIE53_001742 0.74 -0.4 1,966,644 - ABIE53_001742 0.74 -0.2 1,966,653 + ABIE53_001742 0.75 -1.0 1,966,726 - ABIE53_001742 0.80 +3.9 1,966,859 + +0.8 1,966,859 + +1.6 1,966,859 + -0.4 1,966,860 - +0.8 1,966,860 - -1.4 1,966,860 - -0.9 1,966,878 + -0.5 1,966,879 - -0.0 1,966,899 + -0.1 1,966,900 - +1.6 1,966,923 + -0.2 1,966,923 + -0.6 1,966,923 + -0.8 1,966,923 + +0.1 1,966,924 - -1.3 1,966,924 - +0.1 1,966,924 - +0.4 1,967,144 - ABIE53_001743 0.87 +0.1 1,967,144 - ABIE53_001743 0.87 +1.2 1,967,164 - -0.9 1,967,200 + -0.0 1,967,200 + -0.9 1,967,201 - +0.0 1,967,201 - +0.6 1,967,201 - -1.3 1,967,201 - -0.6 1,967,268 + -0.1 1,967,268 + +0.6 1,967,268 + +0.3 1,967,269 - +0.7 1,967,269 - -3.0 1,967,269 - +2.5 1,967,269 - +0.3 1,967,269 - +0.6 1,967,269 - +2.0 1,967,269 - -0.4 1,967,324 - -0.7 1,967,328 + +0.6 1,967,328 + +0.8 1,967,328 + +0.1 1,967,328 + -1.8 1,967,467 + ABIE53_001744 0.16 -0.4 1,967,467 + ABIE53_001744 0.16 -2.1 1,967,467 + ABIE53_001744 0.16 -1.0 1,967,467 + ABIE53_001744 0.16 -0.4 1,967,468 - ABIE53_001744 0.16 +0.1 1,967,468 - ABIE53_001744 0.16 -0.4 1,967,468 - ABIE53_001744 0.16 +0.3 1,967,468 - ABIE53_001744 0.16 -0.0 1,967,471 - ABIE53_001744 0.16 +0.3 1,967,471 - ABIE53_001744 0.16 +3.1 1,967,471 - ABIE53_001744 0.16 +1.2 1,967,625 + ABIE53_001744 0.28 +0.6 1,967,626 - ABIE53_001744 0.28 +1.0 1,967,652 + ABIE53_001744 0.30 -0.3 1,967,653 - ABIE53_001744 0.30 -1.0 1,967,653 - ABIE53_001744 0.30 -0.0 1,967,653 - ABIE53_001744 0.30 -0.2 1,967,653 - ABIE53_001744 0.30 +0.4 1,967,653 - ABIE53_001744 0.30 -1.0 1,967,704 - ABIE53_001744 0.35 +1.4 1,967,733 + ABIE53_001744 0.37 +0.6 1,967,734 - ABIE53_001744 0.37 +0.6 1,967,880 + ABIE53_001744 0.48 +0.4 1,967,881 - ABIE53_001744 0.48 -0.2 1,967,886 + ABIE53_001744 0.49 +0.3 1,967,886 + ABIE53_001744 0.49 -0.6 1,967,887 - ABIE53_001744 0.49 +1.6 1,967,887 - ABIE53_001744 0.49 -0.3 1,967,904 + ABIE53_001744 0.50 -0.8 1,967,904 + ABIE53_001744 0.50 -0.1 1,967,904 + ABIE53_001744 0.50 -0.4 1,967,904 + ABIE53_001744 0.50 +0.6 1,967,904 + ABIE53_001744 0.50 +0.6 1,967,904 + ABIE53_001744 0.50 +1.6 1,967,904 + ABIE53_001744 0.50 +0.4 1,967,905 - ABIE53_001744 0.50 -1.1 1,967,905 - ABIE53_001744 0.50 -0.2 1,967,905 - ABIE53_001744 0.50 +1.2 1,967,905 - ABIE53_001744 0.50 -2.4
Or see this region's nucleotide sequence