Experiment: Recovery broth with Lacidipine 0.4 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS13675 and BBR_RS20795 are separated by 666 nucleotides BBR_RS20795 and BBR_RS20800 are separated by 4 nucleotides BBR_RS20800 and BBR_RS13680 overlap by 28 nucleotides
BBR_RS13675: BBR_RS13675 - glutaredoxin-like protein, at 842,201 to 842,452
_RS13675
BBR_RS20795: BBR_RS20795 - cystathionine gamma-lyase, at 843,119 to 843,286
_RS20795
BBR_RS20800: BBR_RS20800 - hypothetical protein, at 843,291 to 843,473
_RS20800
BBR_RS13680: BBR_RS13680 - SPFH domain-containing protein, at 843,446 to 844,357
_RS13680
Position (kb) 843
844 Strain fitness (log2 ratio) -2
-1
0
1 at 842.353 kb on + strand, within BBR_RS13675 at 842.365 kb on + strand, within BBR_RS13675 at 842.400 kb on + strand, within BBR_RS13675 at 842.408 kb on - strand, within BBR_RS13675 at 842.408 kb on - strand, within BBR_RS13675 at 842.510 kb on + strand at 842.518 kb on - strand at 842.518 kb on - strand at 842.552 kb on + strand at 842.562 kb on + strand at 842.628 kb on + strand at 842.628 kb on + strand at 842.695 kb on + strand at 842.695 kb on + strand at 842.700 kb on + strand at 842.708 kb on - strand at 842.717 kb on + strand at 842.767 kb on - strand at 842.785 kb on + strand at 842.937 kb on + strand at 842.943 kb on - strand at 843.008 kb on + strand at 843.064 kb on + strand at 843.072 kb on - strand at 843.072 kb on - strand at 843.111 kb on - strand at 843.233 kb on - strand, within BBR_RS20795 at 843.244 kb on + strand, within BBR_RS20795 at 843.275 kb on + strand at 843.285 kb on + strand at 843.323 kb on + strand, within BBR_RS20800 at 843.450 kb on - strand at 843.476 kb on + strand at 843.484 kb on - strand at 843.502 kb on + strand at 843.502 kb on + strand at 843.506 kb on + strand at 843.514 kb on - strand at 843.555 kb on - strand, within BBR_RS13680 at 843.599 kb on + strand, within BBR_RS13680 at 843.605 kb on - strand, within BBR_RS13680 at 843.705 kb on + strand, within BBR_RS13680 at 843.722 kb on + strand, within BBR_RS13680 at 843.728 kb on - strand, within BBR_RS13680 at 843.728 kb on - strand, within BBR_RS13680 at 843.762 kb on + strand, within BBR_RS13680 at 843.770 kb on - strand, within BBR_RS13680 at 843.779 kb on - strand, within BBR_RS13680 at 843.796 kb on + strand, within BBR_RS13680 at 843.838 kb on + strand, within BBR_RS13680 at 843.838 kb on + strand, within BBR_RS13680 at 843.838 kb on + strand, within BBR_RS13680 at 843.847 kb on - strand, within BBR_RS13680 at 843.851 kb on + strand, within BBR_RS13680 at 843.884 kb on + strand, within BBR_RS13680 at 843.892 kb on - strand, within BBR_RS13680 at 843.894 kb on - strand, within BBR_RS13680 at 843.929 kb on + strand, within BBR_RS13680 at 843.931 kb on + strand, within BBR_RS13680 at 843.939 kb on - strand, within BBR_RS13680 at 843.939 kb on - strand, within BBR_RS13680 at 843.970 kb on + strand, within BBR_RS13680 at 844.101 kb on + strand, within BBR_RS13680 at 844.107 kb on - strand, within BBR_RS13680 at 844.107 kb on - strand, within BBR_RS13680 at 844.146 kb on - strand, within BBR_RS13680 at 844.153 kb on + strand, within BBR_RS13680 at 844.153 kb on + strand, within BBR_RS13680 at 844.153 kb on + strand, within BBR_RS13680 at 844.153 kb on + strand, within BBR_RS13680 at 844.153 kb on + strand, within BBR_RS13680 at 844.156 kb on - strand, within BBR_RS13680 at 844.161 kb on - strand, within BBR_RS13680 at 844.168 kb on + strand, within BBR_RS13680 at 844.183 kb on + strand, within BBR_RS13680 at 844.191 kb on - strand, within BBR_RS13680 at 844.205 kb on + strand, within BBR_RS13680 at 844.208 kb on - strand, within BBR_RS13680 at 844.208 kb on - strand, within BBR_RS13680 at 844.216 kb on + strand, within BBR_RS13680 at 844.258 kb on + strand, within BBR_RS13680 at 844.258 kb on + strand, within BBR_RS13680 at 844.258 kb on + strand, within BBR_RS13680 at 844.289 kb on - strand at 844.328 kb on + strand at 844.387 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Lacidipine 0.4 mM remove 842,353 + BBR_RS13675 0.60 +1.0 842,365 + BBR_RS13675 0.65 -1.8 842,400 + BBR_RS13675 0.79 -0.6 842,408 - BBR_RS13675 0.82 +0.2 842,408 - BBR_RS13675 0.82 +0.1 842,510 + -0.1 842,518 - +0.6 842,518 - +0.0 842,552 + -0.0 842,562 + +1.0 842,628 + -0.5 842,628 + +0.5 842,695 + +0.9 842,695 + +0.3 842,700 + +0.2 842,708 - +1.0 842,717 + -0.4 842,767 - +0.2 842,785 + +0.4 842,937 + +0.5 842,943 - -0.4 843,008 + +0.3 843,064 + +0.0 843,072 - +1.1 843,072 - +0.2 843,111 - +0.8 843,233 - BBR_RS20795 0.68 +0.4 843,244 + BBR_RS20795 0.74 +0.2 843,275 + +0.2 843,285 + -0.1 843,323 + BBR_RS20800 0.17 -0.5 843,450 - +0.7 843,476 + +0.8 843,484 - -1.4 843,502 + -1.2 843,502 + +0.1 843,506 + +0.5 843,514 - -0.1 843,555 - BBR_RS13680 0.12 +0.1 843,599 + BBR_RS13680 0.17 +0.8 843,605 - BBR_RS13680 0.17 +0.2 843,705 + BBR_RS13680 0.28 +0.6 843,722 + BBR_RS13680 0.30 +1.3 843,728 - BBR_RS13680 0.31 -0.9 843,728 - BBR_RS13680 0.31 -1.0 843,762 + BBR_RS13680 0.35 +0.2 843,770 - BBR_RS13680 0.36 +1.3 843,779 - BBR_RS13680 0.37 -0.3 843,796 + BBR_RS13680 0.38 -0.9 843,838 + BBR_RS13680 0.43 -2.2 843,838 + BBR_RS13680 0.43 -0.3 843,838 + BBR_RS13680 0.43 +0.3 843,847 - BBR_RS13680 0.44 +0.2 843,851 + BBR_RS13680 0.44 +0.1 843,884 + BBR_RS13680 0.48 +0.2 843,892 - BBR_RS13680 0.49 +0.2 843,894 - BBR_RS13680 0.49 +0.2 843,929 + BBR_RS13680 0.53 -0.3 843,931 + BBR_RS13680 0.53 +0.1 843,939 - BBR_RS13680 0.54 +0.5 843,939 - BBR_RS13680 0.54 +0.7 843,970 + BBR_RS13680 0.57 -0.4 844,101 + BBR_RS13680 0.72 -1.8 844,107 - BBR_RS13680 0.72 +0.8 844,107 - BBR_RS13680 0.72 +0.7 844,146 - BBR_RS13680 0.77 +0.9 844,153 + BBR_RS13680 0.78 -0.2 844,153 + BBR_RS13680 0.78 +0.4 844,153 + BBR_RS13680 0.78 -0.3 844,153 + BBR_RS13680 0.78 +0.5 844,153 + BBR_RS13680 0.78 -1.3 844,156 - BBR_RS13680 0.78 +0.1 844,161 - BBR_RS13680 0.78 -0.9 844,168 + BBR_RS13680 0.79 -0.5 844,183 + BBR_RS13680 0.81 +0.4 844,191 - BBR_RS13680 0.82 +0.8 844,205 + BBR_RS13680 0.83 -0.7 844,208 - BBR_RS13680 0.84 +1.0 844,208 - BBR_RS13680 0.84 +0.2 844,216 + BBR_RS13680 0.84 +0.3 844,258 + BBR_RS13680 0.89 +0.1 844,258 + BBR_RS13680 0.89 -1.0 844,258 + BBR_RS13680 0.89 +0.2 844,289 - +0.5 844,328 + -1.1 844,387 + -0.3
Or see this region's nucleotide sequence