Experiment: Recovery broth with Lacidipine 0.4 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS12605 and BBR_RS12610 overlap by 4 nucleotides BBR_RS12610 and BBR_RS20720 overlap by 14 nucleotides BBR_RS20720 and BBR_RS12620 are separated by 27 nucleotides
BBR_RS12605: BBR_RS12605 - glycosyltransferase family 2 protein, at 584,085 to 585,095
_RS12605
BBR_RS12610: BBR_RS12610 - capsular polysaccharide biosynthesis protein, at 585,092 to 586,027
_RS12610
BBR_RS20720: BBR_RS20720 - DUF4422 domain-containing protein, at 586,014 to 586,844
_RS20720
BBR_RS12620: BBR_RS12620 - glycosyltransferase family 1 protein, at 586,872 to 587,945
_RS12620
Position (kb)
586
587 Strain fitness (log2 ratio)
-1
0
1
2 at 585.106 kb on + strand at 585.197 kb on - strand, within BBR_RS12610 at 585.216 kb on - strand, within BBR_RS12610 at 585.274 kb on - strand, within BBR_RS12610 at 585.282 kb on - strand, within BBR_RS12610 at 585.342 kb on + strand, within BBR_RS12610 at 585.355 kb on - strand, within BBR_RS12610 at 585.434 kb on + strand, within BBR_RS12610 at 585.505 kb on + strand, within BBR_RS12610 at 585.605 kb on - strand, within BBR_RS12610 at 585.672 kb on - strand, within BBR_RS12610 at 585.769 kb on - strand, within BBR_RS12610 at 585.778 kb on + strand, within BBR_RS12610 at 585.786 kb on - strand, within BBR_RS12610 at 585.832 kb on + strand, within BBR_RS12610 at 585.896 kb on - strand, within BBR_RS12610 at 585.953 kb on - strand at 585.953 kb on - strand at 585.973 kb on - strand at 585.981 kb on + strand at 585.989 kb on - strand at 585.989 kb on - strand at 585.991 kb on - strand at 586.002 kb on + strand at 586.010 kb on - strand at 586.010 kb on - strand at 586.010 kb on - strand at 586.010 kb on - strand at 586.010 kb on - strand at 586.043 kb on - strand at 586.056 kb on - strand at 586.061 kb on + strand at 586.083 kb on - strand at 586.101 kb on - strand, within BBR_RS20720 at 586.153 kb on + strand, within BBR_RS20720 at 586.153 kb on + strand, within BBR_RS20720 at 586.161 kb on - strand, within BBR_RS20720 at 586.171 kb on + strand, within BBR_RS20720 at 586.240 kb on - strand, within BBR_RS20720 at 586.251 kb on - strand, within BBR_RS20720 at 586.272 kb on - strand, within BBR_RS20720 at 586.366 kb on + strand, within BBR_RS20720 at 586.458 kb on - strand, within BBR_RS20720 at 586.458 kb on - strand, within BBR_RS20720 at 586.502 kb on + strand, within BBR_RS20720 at 586.597 kb on + strand, within BBR_RS20720 at 586.636 kb on - strand, within BBR_RS20720 at 586.636 kb on - strand, within BBR_RS20720 at 586.679 kb on - strand, within BBR_RS20720 at 586.789 kb on + strand at 586.789 kb on - strand at 586.819 kb on - strand at 586.819 kb on - strand at 586.855 kb on - strand at 586.888 kb on + strand at 586.888 kb on + strand at 586.896 kb on - strand at 586.901 kb on - strand at 586.906 kb on + strand at 586.906 kb on - strand at 586.908 kb on + strand at 586.916 kb on - strand at 586.921 kb on - strand at 586.924 kb on - strand at 586.951 kb on + strand at 586.959 kb on - strand at 586.969 kb on - strand at 586.969 kb on - strand at 586.982 kb on - strand, within BBR_RS12620 at 586.983 kb on - strand, within BBR_RS12620 at 586.983 kb on - strand, within BBR_RS12620 at 587.001 kb on - strand, within BBR_RS12620 at 587.039 kb on - strand, within BBR_RS12620 at 587.092 kb on - strand, within BBR_RS12620 at 587.092 kb on - strand, within BBR_RS12620 at 587.109 kb on + strand, within BBR_RS12620 at 587.109 kb on + strand, within BBR_RS12620 at 587.135 kb on - strand, within BBR_RS12620 at 587.135 kb on - strand, within BBR_RS12620 at 587.138 kb on + strand, within BBR_RS12620 at 587.139 kb on + strand, within BBR_RS12620 at 587.139 kb on + strand, within BBR_RS12620 at 587.144 kb on + strand, within BBR_RS12620 at 587.144 kb on + strand, within BBR_RS12620 at 587.152 kb on - strand, within BBR_RS12620 at 587.178 kb on - strand, within BBR_RS12620 at 587.178 kb on - strand, within BBR_RS12620 at 587.227 kb on - strand, within BBR_RS12620 at 587.235 kb on + strand, within BBR_RS12620 at 587.248 kb on + strand, within BBR_RS12620 at 587.248 kb on + strand, within BBR_RS12620 at 587.248 kb on + strand, within BBR_RS12620 at 587.254 kb on + strand, within BBR_RS12620 at 587.256 kb on - strand, within BBR_RS12620 at 587.257 kb on - strand, within BBR_RS12620 at 587.318 kb on - strand, within BBR_RS12620 at 587.338 kb on - strand, within BBR_RS12620 at 587.447 kb on + strand, within BBR_RS12620 at 587.470 kb on - strand, within BBR_RS12620 at 587.488 kb on + strand, within BBR_RS12620 at 587.488 kb on + strand, within BBR_RS12620 at 587.530 kb on + strand, within BBR_RS12620 at 587.530 kb on + strand, within BBR_RS12620 at 587.540 kb on + strand, within BBR_RS12620 at 587.550 kb on + strand, within BBR_RS12620 at 587.555 kb on + strand, within BBR_RS12620 at 587.558 kb on + strand, within BBR_RS12620 at 587.563 kb on + strand, within BBR_RS12620 at 587.571 kb on - strand, within BBR_RS12620 at 587.640 kb on - strand, within BBR_RS12620 at 587.648 kb on - strand, within BBR_RS12620 at 587.704 kb on - strand, within BBR_RS12620 at 587.716 kb on - strand, within BBR_RS12620 at 587.758 kb on - strand, within BBR_RS12620 at 587.787 kb on + strand, within BBR_RS12620 at 587.787 kb on + strand, within BBR_RS12620 at 587.828 kb on - strand, within BBR_RS12620
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Lacidipine 0.4 mM remove 585,106 + +1.4 585,197 - BBR_RS12610 0.11 -0.5 585,216 - BBR_RS12610 0.13 +0.6 585,274 - BBR_RS12610 0.19 -0.4 585,282 - BBR_RS12610 0.20 +2.2 585,342 + BBR_RS12610 0.27 -0.8 585,355 - BBR_RS12610 0.28 -1.0 585,434 + BBR_RS12610 0.37 +0.1 585,505 + BBR_RS12610 0.44 -0.2 585,605 - BBR_RS12610 0.55 -1.2 585,672 - BBR_RS12610 0.62 -0.1 585,769 - BBR_RS12610 0.72 +0.8 585,778 + BBR_RS12610 0.73 +0.8 585,786 - BBR_RS12610 0.74 +0.7 585,832 + BBR_RS12610 0.79 +0.7 585,896 - BBR_RS12610 0.86 +0.5 585,953 - +1.2 585,953 - +0.3 585,973 - +1.1 585,981 + +0.0 585,989 - -0.7 585,989 - -0.7 585,991 - -1.4 586,002 + +0.1 586,010 - -0.3 586,010 - +0.7 586,010 - -1.0 586,010 - -1.2 586,010 - +0.5 586,043 - +0.0 586,056 - +1.9 586,061 + +0.7 586,083 - -0.9 586,101 - BBR_RS20720 0.10 -0.2 586,153 + BBR_RS20720 0.17 -1.0 586,153 + BBR_RS20720 0.17 -0.8 586,161 - BBR_RS20720 0.18 -0.8 586,171 + BBR_RS20720 0.19 +0.1 586,240 - BBR_RS20720 0.27 -0.3 586,251 - BBR_RS20720 0.29 -1.2 586,272 - BBR_RS20720 0.31 +0.0 586,366 + BBR_RS20720 0.42 -1.3 586,458 - BBR_RS20720 0.53 -0.8 586,458 - BBR_RS20720 0.53 +0.2 586,502 + BBR_RS20720 0.59 -0.7 586,597 + BBR_RS20720 0.70 +0.0 586,636 - BBR_RS20720 0.75 +0.9 586,636 - BBR_RS20720 0.75 -0.9 586,679 - BBR_RS20720 0.80 -0.0 586,789 + +0.4 586,789 - +0.4 586,819 - +0.0 586,819 - -1.2 586,855 - +0.2 586,888 + -0.2 586,888 + +1.8 586,896 - +1.0 586,901 - +0.6 586,906 + +0.8 586,906 - +0.5 586,908 + -0.6 586,916 - +0.1 586,921 - -0.3 586,924 - +0.9 586,951 + +1.2 586,959 - +0.8 586,969 - -0.1 586,969 - +0.6 586,982 - BBR_RS12620 0.10 +0.2 586,983 - BBR_RS12620 0.10 -0.4 586,983 - BBR_RS12620 0.10 +0.9 587,001 - BBR_RS12620 0.12 +0.7 587,039 - BBR_RS12620 0.16 -1.0 587,092 - BBR_RS12620 0.20 +0.2 587,092 - BBR_RS12620 0.20 -0.1 587,109 + BBR_RS12620 0.22 +1.0 587,109 + BBR_RS12620 0.22 +1.3 587,135 - BBR_RS12620 0.24 +0.9 587,135 - BBR_RS12620 0.24 +1.3 587,138 + BBR_RS12620 0.25 +0.4 587,139 + BBR_RS12620 0.25 -0.1 587,139 + BBR_RS12620 0.25 -0.9 587,144 + BBR_RS12620 0.25 -1.7 587,144 + BBR_RS12620 0.25 +1.6 587,152 - BBR_RS12620 0.26 +1.3 587,178 - BBR_RS12620 0.28 +0.4 587,178 - BBR_RS12620 0.28 +1.4 587,227 - BBR_RS12620 0.33 +1.3 587,235 + BBR_RS12620 0.34 +0.3 587,248 + BBR_RS12620 0.35 +0.1 587,248 + BBR_RS12620 0.35 +1.3 587,248 + BBR_RS12620 0.35 +0.5 587,254 + BBR_RS12620 0.36 +0.9 587,256 - BBR_RS12620 0.36 +0.6 587,257 - BBR_RS12620 0.36 -0.1 587,318 - BBR_RS12620 0.42 +0.9 587,338 - BBR_RS12620 0.43 +1.5 587,447 + BBR_RS12620 0.54 +1.3 587,470 - BBR_RS12620 0.56 +0.1 587,488 + BBR_RS12620 0.57 -0.2 587,488 + BBR_RS12620 0.57 -0.5 587,530 + BBR_RS12620 0.61 +0.8 587,530 + BBR_RS12620 0.61 -0.8 587,540 + BBR_RS12620 0.62 +1.2 587,550 + BBR_RS12620 0.63 +0.4 587,555 + BBR_RS12620 0.64 +0.5 587,558 + BBR_RS12620 0.64 +0.3 587,563 + BBR_RS12620 0.64 -0.9 587,571 - BBR_RS12620 0.65 +0.5 587,640 - BBR_RS12620 0.72 +0.3 587,648 - BBR_RS12620 0.72 +0.4 587,704 - BBR_RS12620 0.77 +0.5 587,716 - BBR_RS12620 0.79 +0.7 587,758 - BBR_RS12620 0.82 -0.1 587,787 + BBR_RS12620 0.85 +1.1 587,787 + BBR_RS12620 0.85 +0.4 587,828 - BBR_RS12620 0.89 -0.1
Or see this region's nucleotide sequence