Strain Fitness in Bifidobacterium breve UCC2003 around BBR_RS20465

Experiment: Recovery broth with Lacidipine 0.4 mM

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Recovery broth with Lacidipine 0.4 mM
2,416,898	+		BBR_RS20460	0.24	-0.4
2,416,911	-		BBR_RS20460	0.26	-1.3
2,416,968	-		BBR_RS20460	0.30	-1.3
2,416,978	-		BBR_RS20460	0.31	+0.2
2,417,082	+		BBR_RS20460	0.39	+1.1
2,417,112	-		BBR_RS20460	0.41	-1.7
2,417,146	+		BBR_RS20460	0.44	+0.3
2,417,187	-		BBR_RS20460	0.47	-0.0
2,417,216	+		BBR_RS20460	0.50	+0.2
2,417,236	+		BBR_RS20460	0.51	-0.1
2,417,304	-		BBR_RS20460	0.57	+0.1
2,417,309	-		BBR_RS20460	0.57	+0.1
2,417,311	-		BBR_RS20460	0.57	+0.1
2,417,361	-		BBR_RS20460	0.61	-0.9
2,417,363	+		BBR_RS20460	0.61	+2.5
2,417,479	-		BBR_RS20460	0.70	-2.1
2,417,497	-		BBR_RS20460	0.72	-1.5
2,417,530	-		BBR_RS20460	0.74	-1.4
2,417,723	-		BBR_RS20460	0.90	-0.6
2,417,738	+				-2.2
2,417,801	-				-1.2
2,417,838	-				-1.1
2,417,838	-				-0.2
2,417,860	-				-0.3
2,417,867	+				-0.6
2,417,875	+				+0.4
2,417,875	-				+0.2
2,417,875	-				-1.8
2,417,875	-				+0.9
2,417,883	-				-1.6
2,417,883	-				-0.6
2,417,930	-				-0.3
2,417,944	-				-1.5
2,417,946	-				-1.5
2,418,024	-		BBR_RS20465	0.18	+1.1
2,418,039	-		BBR_RS20465	0.19	-0.2
2,418,039	-		BBR_RS20465	0.19	-0.4
2,418,054	-		BBR_RS20465	0.21	-1.2
2,418,054	-		BBR_RS20465	0.21	+0.8
2,418,065	+		BBR_RS20465	0.22	-0.1
2,418,065	-		BBR_RS20465	0.22	-0.6
2,418,066	-		BBR_RS20465	0.22	-1.0
2,418,072	+		BBR_RS20465	0.23	-0.8
2,418,078	-		BBR_RS20465	0.23	+0.3
2,418,078	-		BBR_RS20465	0.23	-1.9
2,418,078	-		BBR_RS20465	0.23	-1.4
2,418,080	+		BBR_RS20465	0.23	-0.9
2,418,080	-		BBR_RS20465	0.23	+2.4
2,418,080	-		BBR_RS20465	0.23	-0.7
2,418,080	-		BBR_RS20465	0.23	-1.7
2,418,080	-		BBR_RS20465	0.23	+0.6
2,418,080	-		BBR_RS20465	0.23	-0.5
2,418,080	-		BBR_RS20465	0.23	-2.4
2,418,080	-		BBR_RS20465	0.23	+0.6
2,418,080	-		BBR_RS20465	0.23	-0.1
2,418,080	-		BBR_RS20465	0.23	-1.5
2,418,088	-		BBR_RS20465	0.24	-1.1
2,418,088	-		BBR_RS20465	0.24	-0.5
2,418,088	-		BBR_RS20465	0.24	-0.1
2,418,104	-		BBR_RS20465	0.26	-0.9
2,418,115	-		BBR_RS20465	0.27	-1.2
2,418,154	-		BBR_RS20465	0.31	-1.6
2,418,154	-		BBR_RS20465	0.31	+1.1
2,418,160	-		BBR_RS20465	0.32	+0.1
2,418,160	-		BBR_RS20465	0.32	-1.3
2,418,168	-		BBR_RS20465	0.33	-0.9
2,418,168	-		BBR_RS20465	0.33	-0.6
2,418,181	-		BBR_RS20465	0.34	-0.4
2,418,182	-		BBR_RS20465	0.34	-2.0
2,418,191	-		BBR_RS20465	0.35	-1.4
2,418,272	-		BBR_RS20465	0.43	-1.3
2,418,294	-		BBR_RS20465	0.46	+1.8
2,418,307	-		BBR_RS20465	0.47	-1.2
2,418,309	-		BBR_RS20465	0.47	-1.4
2,418,377	+		BBR_RS20465	0.54	-0.4
2,418,447	-		BBR_RS20465	0.61	+0.6
2,418,447	-		BBR_RS20465	0.61	-1.1
2,418,547	-		BBR_RS20465	0.72	-1.7
2,418,556	-		BBR_RS20465	0.73	-2.0
2,418,579	-		BBR_RS20465	0.75	+1.5
2,418,579	-		BBR_RS20465	0.75	-0.7
2,418,584	-		BBR_RS20465	0.75	+0.1
2,418,607	-		BBR_RS20465	0.78	-1.8
2,418,624	-		BBR_RS20465	0.80	-1.7
2,418,657	+		BBR_RS20465	0.83	-0.3
2,418,659	-		BBR_RS20465	0.83	-2.2
2,418,659	-		BBR_RS20465	0.83	+0.5
2,418,664	+		BBR_RS20465	0.84	-1.4
2,418,672	-		BBR_RS20465	0.85	-0.2
2,418,727	+				-0.1
2,418,727	+				+0.6
2,418,727	+				-1.0
2,418,735	-				-2.0
2,418,735	-				-0.1
2,418,745	-				-1.6
2,418,745	-				-2.5
2,418,798	+				-0.2
2,418,817	+				+2.1
2,418,822	-				-1.4
2,418,828	+				+0.0
2,418,836	-				-1.3
2,418,844	+				-1.0
2,418,846	-				-0.8
2,418,850	+				+0.3
2,418,852	+				+0.8
2,418,852	+				-0.4
2,418,852	-				-0.7
2,418,852	-				-0.1
2,418,872	+				-2.3
2,418,882	+				+0.1
2,418,897	+				-0.8
2,418,900	-				-2.0
2,418,907	+				-1.1
2,418,907	+				-0.7
2,418,907	+				+0.3
2,418,921	-				-0.1
2,418,931	-				+0.0
2,418,946	-				-1.2
2,418,946	-				-0.8
2,419,012	+				+0.7
2,419,012	-				-0.3
2,419,046	-				+0.4
2,419,046	-				+0.3
2,419,068	-				-2.6
2,419,079	-				+0.3
2,419,112	+				+0.2
2,419,114	-				-0.3
2,419,115	-				+1.2
2,419,117	+				-0.3
2,419,120	-				+0.3
2,419,120	-				+0.1
2,419,120	-				-0.8
2,419,125	-				+0.8
2,419,125	-				-0.3
2,419,146	-		BBR_RS20470	0.11	+0.6
2,419,151	+		BBR_RS20470	0.12	+0.1
2,419,159	-		BBR_RS20470	0.13	+0.1
2,419,175	-		BBR_RS20470	0.15	+0.2
2,419,175	-		BBR_RS20470	0.15	-0.7
2,419,175	-		BBR_RS20470	0.15	-2.2
2,419,180	+		BBR_RS20470	0.16	-0.5
2,419,185	+		BBR_RS20470	0.17	-0.2
2,419,187	-		BBR_RS20470	0.17	+0.2
2,419,188	-		BBR_RS20470	0.17	+0.2
2,419,193	-		BBR_RS20470	0.18	-0.1
2,419,198	-		BBR_RS20470	0.19	+0.3
2,419,204	+		BBR_RS20470	0.20	+1.2
2,419,209	+		BBR_RS20470	0.20	+0.3
2,419,218	-		BBR_RS20470	0.22	+0.2
2,419,220	-		BBR_RS20470	0.22	+1.1
2,419,220	-		BBR_RS20470	0.22	-1.5
2,419,237	+		BBR_RS20470	0.24	-1.1
2,419,245	-		BBR_RS20470	0.26	+0.1
2,419,249	+		BBR_RS20470	0.26	-0.9
2,419,249	+		BBR_RS20470	0.26	+0.3
2,419,249	+		BBR_RS20470	0.26	-0.3
2,419,255	-		BBR_RS20470	0.27	+0.0
2,419,256	-		BBR_RS20470	0.27	-0.7
2,419,257	-		BBR_RS20470	0.27	-2.6
2,419,257	-		BBR_RS20470	0.27	+0.6
2,419,269	-		BBR_RS20470	0.29	+0.7
2,419,286	+		BBR_RS20470	0.32	+0.1
2,419,286	+		BBR_RS20470	0.32	+2.6
2,419,294	-		BBR_RS20470	0.33	-1.1
2,419,294	-		BBR_RS20470	0.33	+0.3
2,419,294	-		BBR_RS20470	0.33	-0.9
2,419,294	-		BBR_RS20470	0.33	+0.4
2,419,302	-		BBR_RS20470	0.34	-1.8
2,419,304	-		BBR_RS20470	0.35	+0.9
2,419,325	-		BBR_RS20470	0.38	+0.8
2,419,326	+		BBR_RS20470	0.38	-0.7
2,419,327	-		BBR_RS20470	0.38	+0.3
2,419,334	+		BBR_RS20470	0.39	-2.0
2,419,336	+		BBR_RS20470	0.39	+0.7
2,419,336	+		BBR_RS20470	0.39	-1.0
2,419,342	-		BBR_RS20470	0.40	-1.5
2,419,344	-		BBR_RS20470	0.41	-0.3
2,419,346	-		BBR_RS20470	0.41	-0.5
2,419,354	-		BBR_RS20470	0.42	-0.7
2,419,372	-		BBR_RS20470	0.45	-0.9
2,419,372	-		BBR_RS20470	0.45	-0.4
2,419,380	+		BBR_RS20470	0.46	-0.8
2,419,388	-		BBR_RS20470	0.47	+0.0
2,419,389	+		BBR_RS20470	0.47	-0.3
2,419,389	+		BBR_RS20470	0.47	-1.6
2,419,394	+		BBR_RS20470	0.48	-0.1
2,419,394	+		BBR_RS20470	0.48	-0.7
2,419,397	-		BBR_RS20470	0.48	+0.3
2,419,406	+		BBR_RS20470	0.50	+0.7
2,419,414	-		BBR_RS20470	0.51	-0.0
2,419,414	-		BBR_RS20470	0.51	-1.7
2,419,414	-		BBR_RS20470	0.51	-0.6
2,419,414	-		BBR_RS20470	0.51	-0.8
2,419,421	-		BBR_RS20470	0.52	-0.9
2,419,421	-		BBR_RS20470	0.52	-0.3
2,419,421	-		BBR_RS20470	0.52	-0.0
2,419,423	-		BBR_RS20470	0.52	-0.2
2,419,425	+		BBR_RS20470	0.53	+0.2
2,419,433	-		BBR_RS20470	0.54	-0.7
2,419,433	-		BBR_RS20470	0.54	-2.1
2,419,435	+		BBR_RS20470	0.54	-1.4
2,419,435	+		BBR_RS20470	0.54	+0.2
2,419,435	+		BBR_RS20470	0.54	-0.1
2,419,443	-		BBR_RS20470	0.55	-1.0
2,419,443	-		BBR_RS20470	0.55	+0.5
2,419,453	-		BBR_RS20470	0.57	+0.2
2,419,453	-		BBR_RS20470	0.57	+0.6
2,419,453	-		BBR_RS20470	0.57	-0.9
2,419,479	+		BBR_RS20470	0.61	-0.2
2,419,487	-		BBR_RS20470	0.62	-0.9
2,419,487	-		BBR_RS20470	0.62	+0.2
2,419,489	+		BBR_RS20470	0.62	+0.7
2,419,489	+		BBR_RS20470	0.62	-1.7
2,419,492	-		BBR_RS20470	0.63	-1.5
2,419,494	+		BBR_RS20470	0.63	-0.3
2,419,494	+		BBR_RS20470	0.63	-0.5
2,419,496	+		BBR_RS20470	0.63	+0.3
2,419,502	-		BBR_RS20470	0.64	-0.9
2,419,503	-		BBR_RS20470	0.64	+0.2
2,419,504	+		BBR_RS20470	0.65	+0.3
2,419,511	+		BBR_RS20470	0.66	+1.4
2,419,511	+		BBR_RS20470	0.66	+1.1
2,419,512	-		BBR_RS20470	0.66	-0.8
2,419,512	-		BBR_RS20470	0.66	+0.3
2,419,514	+		BBR_RS20470	0.66	+0.5
2,419,522	+		BBR_RS20470	0.67	-2.4
2,419,530	-		BBR_RS20470	0.68	+0.5
2,419,540	+		BBR_RS20470	0.70	+0.5
2,419,546	+		BBR_RS20470	0.71	+0.1
2,419,547	-		BBR_RS20470	0.71	-0.2
2,419,554	-		BBR_RS20470	0.72	-1.0
2,419,556	+		BBR_RS20470	0.72	-1.5
2,419,565	-		BBR_RS20470	0.74	-1.4
2,419,565	-		BBR_RS20470	0.74	+0.2
2,419,565	-		BBR_RS20470	0.74	-1.3
2,419,565	-		BBR_RS20470	0.74	-0.4
2,419,605	-		BBR_RS20470	0.80	+0.5
2,419,619	+		BBR_RS20470	0.82	+1.8
2,419,637	+		BBR_RS20470	0.85	+0.3
2,419,637	+		BBR_RS20470	0.85	-0.1
2,419,643	+		BBR_RS20470	0.85	+0.2
2,419,645	-		BBR_RS20470	0.86	+0.7
2,419,645	-		BBR_RS20470	0.86	+1.1
2,419,646	+		BBR_RS20470	0.86	-0.6
2,419,653	-		BBR_RS20470	0.87	+0.9
2,419,683	-				+1.4
2,419,687	+				+0.4
2,419,687	+				+0.0
2,419,688	-				+0.5
2,419,688	-				-2.1
2,419,688	-				-0.6
2,419,688	-				-0.9
2,419,688	-				-1.8
2,419,692	+				-0.3
2,419,692	+				+0.1
2,419,694	+				-1.0
2,419,695	-				-0.7
2,419,700	-				-0.2
2,419,700	-				+0.4
2,419,700	-				-0.1
2,419,702	-				-0.4
2,419,704	+				+0.2
2,419,704	+				+0.0
2,419,712	-				+0.3
2,419,721	+				+0.2
2,419,728	+				-2.3
2,419,728	-				-1.2
2,419,734	+				-0.5
2,419,734	+				-0.2
2,419,735	+				-1.6
2,419,739	+				-0.9
2,419,742	-				-0.3
2,419,742	-				-0.5
2,419,742	-				-0.0
2,419,744	-				+0.5
2,419,748	+				-0.3
2,419,748	+				-0.7
2,419,757	-				-0.1
2,419,757	-				-0.9
2,419,759	-				+1.7
2,419,778	-				-0.8
2,419,779	+				+0.1
2,419,779	+				+0.4
2,419,781	+				-0.3
2,419,787	-				-0.8
2,419,788	-				+0.0
2,419,788	-				+1.1
2,419,800	-				+1.0
2,419,800	-				-1.1
2,419,813	-				-0.9
2,419,818	-				-0.3