Experiment: Recovery broth with Lacidipine 0.4 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS20455 and BBR_RS20460 are separated by 116 nucleotides BBR_RS20460 and BBR_RS20465 overlap by 1 nucleotides
BBR_RS20455: BBR_RS20455 - thioredoxin-disulfide reductase, at 2,415,452 to 2,416,471
_RS20455
BBR_RS20460: BBR_RS20460 - chromosome partitioning protein ParB, at 2,416,588 to 2,417,853
_RS20460
BBR_RS20465: BBR_RS20465 - chromosome partitioning protein, at 2,417,853 to 2,418,821
_RS20465
Position (kb)
2416
2417
2418 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2415.590 kb on - strand, within BBR_RS20455 at 2415.635 kb on - strand, within BBR_RS20455 at 2415.687 kb on - strand, within BBR_RS20455 at 2415.695 kb on + strand, within BBR_RS20455 at 2415.708 kb on + strand, within BBR_RS20455 at 2415.712 kb on + strand, within BBR_RS20455 at 2415.740 kb on + strand, within BBR_RS20455 at 2415.740 kb on + strand, within BBR_RS20455 at 2415.748 kb on - strand, within BBR_RS20455 at 2415.758 kb on - strand, within BBR_RS20455 at 2415.768 kb on - strand, within BBR_RS20455 at 2415.861 kb on + strand, within BBR_RS20455 at 2415.861 kb on - strand, within BBR_RS20455 at 2415.871 kb on + strand, within BBR_RS20455 at 2415.920 kb on - strand, within BBR_RS20455 at 2415.965 kb on - strand, within BBR_RS20455 at 2416.010 kb on - strand, within BBR_RS20455 at 2416.015 kb on - strand, within BBR_RS20455 at 2416.028 kb on - strand, within BBR_RS20455 at 2416.056 kb on - strand, within BBR_RS20455 at 2416.058 kb on + strand, within BBR_RS20455 at 2416.072 kb on + strand, within BBR_RS20455 at 2416.072 kb on + strand, within BBR_RS20455 at 2416.080 kb on - strand, within BBR_RS20455 at 2416.105 kb on + strand, within BBR_RS20455 at 2416.128 kb on - strand, within BBR_RS20455 at 2416.141 kb on - strand, within BBR_RS20455 at 2416.157 kb on - strand, within BBR_RS20455 at 2416.191 kb on + strand, within BBR_RS20455 at 2416.235 kb on - strand, within BBR_RS20455 at 2416.267 kb on + strand, within BBR_RS20455 at 2416.271 kb on - strand, within BBR_RS20455 at 2416.272 kb on + strand, within BBR_RS20455 at 2416.275 kb on - strand, within BBR_RS20455 at 2416.297 kb on + strand, within BBR_RS20455 at 2416.302 kb on + strand, within BBR_RS20455 at 2416.305 kb on - strand, within BBR_RS20455 at 2416.305 kb on - strand, within BBR_RS20455 at 2416.340 kb on + strand, within BBR_RS20455 at 2416.340 kb on + strand, within BBR_RS20455 at 2416.344 kb on - strand, within BBR_RS20455 at 2416.346 kb on - strand, within BBR_RS20455 at 2416.364 kb on - strand, within BBR_RS20455 at 2416.401 kb on - strand at 2416.428 kb on - strand at 2416.428 kb on - strand at 2416.428 kb on - strand at 2416.439 kb on + strand at 2416.440 kb on + strand at 2416.446 kb on + strand at 2416.453 kb on + strand at 2416.456 kb on + strand at 2416.456 kb on + strand at 2416.456 kb on + strand at 2416.457 kb on + strand at 2416.461 kb on - strand at 2416.464 kb on + strand at 2416.478 kb on + strand at 2416.478 kb on - strand at 2416.480 kb on + strand at 2416.480 kb on + strand at 2416.488 kb on - strand at 2416.488 kb on - strand at 2416.495 kb on + strand at 2416.497 kb on + strand at 2416.497 kb on + strand at 2416.497 kb on + strand at 2416.497 kb on + strand at 2416.497 kb on + strand at 2416.498 kb on - strand at 2416.505 kb on + strand at 2416.505 kb on - strand at 2416.505 kb on - strand at 2416.505 kb on - strand at 2416.505 kb on - strand at 2416.505 kb on - strand at 2416.505 kb on - strand at 2416.505 kb on - strand at 2416.505 kb on - strand at 2416.505 kb on - strand at 2416.505 kb on - strand at 2416.508 kb on - strand at 2416.510 kb on + strand at 2416.513 kb on - strand at 2416.513 kb on - strand at 2416.518 kb on - strand at 2416.518 kb on - strand at 2416.523 kb on - strand at 2416.523 kb on - strand at 2416.523 kb on - strand at 2416.523 kb on - strand at 2416.523 kb on - strand at 2416.524 kb on + strand at 2416.537 kb on + strand at 2416.542 kb on + strand at 2416.542 kb on + strand at 2416.542 kb on - strand at 2416.542 kb on - strand at 2416.542 kb on - strand at 2416.550 kb on - strand at 2416.550 kb on - strand at 2416.550 kb on - strand at 2416.550 kb on - strand at 2416.552 kb on + strand at 2416.556 kb on + strand at 2416.561 kb on + strand at 2416.561 kb on + strand at 2416.564 kb on - strand at 2416.564 kb on - strand at 2416.570 kb on + strand at 2416.578 kb on - strand at 2416.578 kb on - strand at 2416.578 kb on - strand at 2416.578 kb on - strand at 2416.593 kb on - strand at 2416.597 kb on + strand at 2416.605 kb on - strand at 2416.607 kb on + strand at 2416.615 kb on - strand at 2416.615 kb on - strand at 2416.615 kb on - strand at 2416.625 kb on - strand at 2416.625 kb on - strand at 2416.707 kb on - strand at 2416.743 kb on + strand, within BBR_RS20460 at 2416.825 kb on - strand, within BBR_RS20460 at 2416.898 kb on + strand, within BBR_RS20460 at 2416.911 kb on - strand, within BBR_RS20460 at 2416.968 kb on - strand, within BBR_RS20460 at 2416.978 kb on - strand, within BBR_RS20460 at 2417.082 kb on + strand, within BBR_RS20460 at 2417.112 kb on - strand, within BBR_RS20460 at 2417.146 kb on + strand, within BBR_RS20460 at 2417.187 kb on - strand, within BBR_RS20460 at 2417.216 kb on + strand, within BBR_RS20460 at 2417.236 kb on + strand, within BBR_RS20460 at 2417.304 kb on - strand, within BBR_RS20460 at 2417.309 kb on - strand, within BBR_RS20460 at 2417.311 kb on - strand, within BBR_RS20460 at 2417.361 kb on - strand, within BBR_RS20460 at 2417.363 kb on + strand, within BBR_RS20460 at 2417.479 kb on - strand, within BBR_RS20460 at 2417.497 kb on - strand, within BBR_RS20460 at 2417.530 kb on - strand, within BBR_RS20460 at 2417.723 kb on - strand, within BBR_RS20460 at 2417.738 kb on + strand at 2417.801 kb on - strand at 2417.838 kb on - strand at 2417.838 kb on - strand at 2417.860 kb on - strand at 2417.867 kb on + strand at 2417.875 kb on + strand at 2417.875 kb on - strand at 2417.875 kb on - strand at 2417.875 kb on - strand at 2417.883 kb on - strand at 2417.883 kb on - strand at 2417.930 kb on - strand at 2417.944 kb on - strand at 2417.946 kb on - strand at 2418.024 kb on - strand, within BBR_RS20465 at 2418.039 kb on - strand, within BBR_RS20465 at 2418.039 kb on - strand, within BBR_RS20465 at 2418.054 kb on - strand, within BBR_RS20465 at 2418.054 kb on - strand, within BBR_RS20465 at 2418.065 kb on + strand, within BBR_RS20465 at 2418.065 kb on - strand, within BBR_RS20465 at 2418.066 kb on - strand, within BBR_RS20465 at 2418.072 kb on + strand, within BBR_RS20465 at 2418.078 kb on - strand, within BBR_RS20465 at 2418.078 kb on - strand, within BBR_RS20465 at 2418.078 kb on - strand, within BBR_RS20465 at 2418.080 kb on + strand, within BBR_RS20465 at 2418.080 kb on - strand, within BBR_RS20465 at 2418.080 kb on - strand, within BBR_RS20465 at 2418.080 kb on - strand, within BBR_RS20465 at 2418.080 kb on - strand, within BBR_RS20465 at 2418.080 kb on - strand, within BBR_RS20465 at 2418.080 kb on - strand, within BBR_RS20465 at 2418.080 kb on - strand, within BBR_RS20465 at 2418.080 kb on - strand, within BBR_RS20465 at 2418.080 kb on - strand, within BBR_RS20465 at 2418.088 kb on - strand, within BBR_RS20465 at 2418.088 kb on - strand, within BBR_RS20465 at 2418.088 kb on - strand, within BBR_RS20465 at 2418.104 kb on - strand, within BBR_RS20465 at 2418.115 kb on - strand, within BBR_RS20465 at 2418.154 kb on - strand, within BBR_RS20465 at 2418.154 kb on - strand, within BBR_RS20465 at 2418.160 kb on - strand, within BBR_RS20465 at 2418.160 kb on - strand, within BBR_RS20465 at 2418.168 kb on - strand, within BBR_RS20465 at 2418.168 kb on - strand, within BBR_RS20465 at 2418.181 kb on - strand, within BBR_RS20465 at 2418.182 kb on - strand, within BBR_RS20465 at 2418.191 kb on - strand, within BBR_RS20465 at 2418.272 kb on - strand, within BBR_RS20465 at 2418.294 kb on - strand, within BBR_RS20465 at 2418.307 kb on - strand, within BBR_RS20465 at 2418.309 kb on - strand, within BBR_RS20465 at 2418.377 kb on + strand, within BBR_RS20465 at 2418.447 kb on - strand, within BBR_RS20465 at 2418.447 kb on - strand, within BBR_RS20465 at 2418.547 kb on - strand, within BBR_RS20465 at 2418.556 kb on - strand, within BBR_RS20465 at 2418.579 kb on - strand, within BBR_RS20465 at 2418.579 kb on - strand, within BBR_RS20465 at 2418.584 kb on - strand, within BBR_RS20465 at 2418.607 kb on - strand, within BBR_RS20465 at 2418.624 kb on - strand, within BBR_RS20465 at 2418.657 kb on + strand, within BBR_RS20465 at 2418.659 kb on - strand, within BBR_RS20465 at 2418.659 kb on - strand, within BBR_RS20465 at 2418.664 kb on + strand, within BBR_RS20465 at 2418.672 kb on - strand, within BBR_RS20465 at 2418.727 kb on + strand at 2418.727 kb on + strand at 2418.727 kb on + strand at 2418.735 kb on - strand at 2418.735 kb on - strand at 2418.745 kb on - strand at 2418.745 kb on - strand at 2418.798 kb on + strand at 2418.817 kb on + strand at 2418.822 kb on - strand at 2418.828 kb on + strand at 2418.836 kb on - strand at 2418.844 kb on + strand at 2418.846 kb on - strand at 2418.850 kb on + strand at 2418.852 kb on + strand at 2418.852 kb on + strand at 2418.852 kb on - strand at 2418.852 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Lacidipine 0.4 mM remove 2,415,590 - BBR_RS20455 0.14 -0.0 2,415,635 - BBR_RS20455 0.18 +0.4 2,415,687 - BBR_RS20455 0.23 +1.4 2,415,695 + BBR_RS20455 0.24 +0.6 2,415,708 + BBR_RS20455 0.25 +0.2 2,415,712 + BBR_RS20455 0.25 -1.9 2,415,740 + BBR_RS20455 0.28 -1.2 2,415,740 + BBR_RS20455 0.28 -0.3 2,415,748 - BBR_RS20455 0.29 -1.5 2,415,758 - BBR_RS20455 0.30 -1.0 2,415,768 - BBR_RS20455 0.31 -1.0 2,415,861 + BBR_RS20455 0.40 +0.4 2,415,861 - BBR_RS20455 0.40 +0.4 2,415,871 + BBR_RS20455 0.41 +0.8 2,415,920 - BBR_RS20455 0.46 -0.6 2,415,965 - BBR_RS20455 0.50 -2.4 2,416,010 - BBR_RS20455 0.55 -1.5 2,416,015 - BBR_RS20455 0.55 +1.1 2,416,028 - BBR_RS20455 0.56 -2.1 2,416,056 - BBR_RS20455 0.59 -1.3 2,416,058 + BBR_RS20455 0.59 +0.1 2,416,072 + BBR_RS20455 0.61 -0.8 2,416,072 + BBR_RS20455 0.61 +1.1 2,416,080 - BBR_RS20455 0.62 -0.0 2,416,105 + BBR_RS20455 0.64 -0.5 2,416,128 - BBR_RS20455 0.66 -0.2 2,416,141 - BBR_RS20455 0.68 -0.1 2,416,157 - BBR_RS20455 0.69 +0.3 2,416,191 + BBR_RS20455 0.72 -0.6 2,416,235 - BBR_RS20455 0.77 -1.7 2,416,267 + BBR_RS20455 0.80 -1.2 2,416,271 - BBR_RS20455 0.80 -1.2 2,416,272 + BBR_RS20455 0.80 +0.5 2,416,275 - BBR_RS20455 0.81 -0.1 2,416,297 + BBR_RS20455 0.83 -1.3 2,416,302 + BBR_RS20455 0.83 -0.1 2,416,305 - BBR_RS20455 0.84 -0.9 2,416,305 - BBR_RS20455 0.84 +0.2 2,416,340 + BBR_RS20455 0.87 -0.3 2,416,340 + BBR_RS20455 0.87 -0.6 2,416,344 - BBR_RS20455 0.87 +0.4 2,416,346 - BBR_RS20455 0.88 -0.5 2,416,364 - BBR_RS20455 0.89 -0.5 2,416,401 - +0.6 2,416,428 - +1.2 2,416,428 - -1.3 2,416,428 - +0.6 2,416,439 + -0.2 2,416,440 + +0.6 2,416,446 + -1.1 2,416,453 + -0.2 2,416,456 + +0.5 2,416,456 + -2.4 2,416,456 + +0.0 2,416,457 + -1.0 2,416,461 - +0.5 2,416,464 + +0.4 2,416,478 + +0.1 2,416,478 - +0.5 2,416,480 + -0.0 2,416,480 + -0.1 2,416,488 - +0.4 2,416,488 - +0.7 2,416,495 + -0.1 2,416,497 + +0.3 2,416,497 + +0.5 2,416,497 + -0.3 2,416,497 + -0.1 2,416,497 + +0.4 2,416,498 - -0.8 2,416,505 + -0.8 2,416,505 - +0.2 2,416,505 - -0.2 2,416,505 - -1.0 2,416,505 - -0.4 2,416,505 - -1.0 2,416,505 - +0.3 2,416,505 - +0.5 2,416,505 - +0.3 2,416,505 - -0.1 2,416,505 - +0.0 2,416,508 - -0.4 2,416,510 + +0.3 2,416,513 - +0.1 2,416,513 - +0.4 2,416,518 - +0.5 2,416,518 - -0.4 2,416,523 - -0.6 2,416,523 - -0.3 2,416,523 - +0.5 2,416,523 - -0.6 2,416,523 - -0.5 2,416,524 + +0.1 2,416,537 + -0.1 2,416,542 + +0.3 2,416,542 + -0.1 2,416,542 - -0.8 2,416,542 - +0.1 2,416,542 - -2.8 2,416,550 - +0.3 2,416,550 - +0.0 2,416,550 - -0.4 2,416,550 - +0.2 2,416,552 + -0.6 2,416,556 + -0.2 2,416,561 + +0.6 2,416,561 + -0.4 2,416,564 - -1.5 2,416,564 - +0.1 2,416,570 + -0.1 2,416,578 - -0.5 2,416,578 - +0.6 2,416,578 - -0.6 2,416,578 - +0.0 2,416,593 - -0.5 2,416,597 + -0.9 2,416,605 - +1.4 2,416,607 + +0.5 2,416,615 - -1.6 2,416,615 - -0.3 2,416,615 - -0.3 2,416,625 - -0.6 2,416,625 - -0.1 2,416,707 - -0.8 2,416,743 + BBR_RS20460 0.12 +0.7 2,416,825 - BBR_RS20460 0.19 -0.5 2,416,898 + BBR_RS20460 0.24 -0.4 2,416,911 - BBR_RS20460 0.26 -1.3 2,416,968 - BBR_RS20460 0.30 -1.3 2,416,978 - BBR_RS20460 0.31 +0.2 2,417,082 + BBR_RS20460 0.39 +1.1 2,417,112 - BBR_RS20460 0.41 -1.7 2,417,146 + BBR_RS20460 0.44 +0.3 2,417,187 - BBR_RS20460 0.47 -0.0 2,417,216 + BBR_RS20460 0.50 +0.2 2,417,236 + BBR_RS20460 0.51 -0.1 2,417,304 - BBR_RS20460 0.57 +0.1 2,417,309 - BBR_RS20460 0.57 +0.1 2,417,311 - BBR_RS20460 0.57 +0.1 2,417,361 - BBR_RS20460 0.61 -0.9 2,417,363 + BBR_RS20460 0.61 +2.5 2,417,479 - BBR_RS20460 0.70 -2.1 2,417,497 - BBR_RS20460 0.72 -1.5 2,417,530 - BBR_RS20460 0.74 -1.4 2,417,723 - BBR_RS20460 0.90 -0.6 2,417,738 + -2.2 2,417,801 - -1.2 2,417,838 - -1.1 2,417,838 - -0.2 2,417,860 - -0.3 2,417,867 + -0.6 2,417,875 + +0.4 2,417,875 - +0.2 2,417,875 - -1.8 2,417,875 - +0.9 2,417,883 - -1.6 2,417,883 - -0.6 2,417,930 - -0.3 2,417,944 - -1.5 2,417,946 - -1.5 2,418,024 - BBR_RS20465 0.18 +1.1 2,418,039 - BBR_RS20465 0.19 -0.2 2,418,039 - BBR_RS20465 0.19 -0.4 2,418,054 - BBR_RS20465 0.21 -1.2 2,418,054 - BBR_RS20465 0.21 +0.8 2,418,065 + BBR_RS20465 0.22 -0.1 2,418,065 - BBR_RS20465 0.22 -0.6 2,418,066 - BBR_RS20465 0.22 -1.0 2,418,072 + BBR_RS20465 0.23 -0.8 2,418,078 - BBR_RS20465 0.23 +0.3 2,418,078 - BBR_RS20465 0.23 -1.9 2,418,078 - BBR_RS20465 0.23 -1.4 2,418,080 + BBR_RS20465 0.23 -0.9 2,418,080 - BBR_RS20465 0.23 +2.4 2,418,080 - BBR_RS20465 0.23 -0.7 2,418,080 - BBR_RS20465 0.23 -1.7 2,418,080 - BBR_RS20465 0.23 +0.6 2,418,080 - BBR_RS20465 0.23 -0.5 2,418,080 - BBR_RS20465 0.23 -2.4 2,418,080 - BBR_RS20465 0.23 +0.6 2,418,080 - BBR_RS20465 0.23 -0.1 2,418,080 - BBR_RS20465 0.23 -1.5 2,418,088 - BBR_RS20465 0.24 -1.1 2,418,088 - BBR_RS20465 0.24 -0.5 2,418,088 - BBR_RS20465 0.24 -0.1 2,418,104 - BBR_RS20465 0.26 -0.9 2,418,115 - BBR_RS20465 0.27 -1.2 2,418,154 - BBR_RS20465 0.31 -1.6 2,418,154 - BBR_RS20465 0.31 +1.1 2,418,160 - BBR_RS20465 0.32 +0.1 2,418,160 - BBR_RS20465 0.32 -1.3 2,418,168 - BBR_RS20465 0.33 -0.9 2,418,168 - BBR_RS20465 0.33 -0.6 2,418,181 - BBR_RS20465 0.34 -0.4 2,418,182 - BBR_RS20465 0.34 -2.0 2,418,191 - BBR_RS20465 0.35 -1.4 2,418,272 - BBR_RS20465 0.43 -1.3 2,418,294 - BBR_RS20465 0.46 +1.8 2,418,307 - BBR_RS20465 0.47 -1.2 2,418,309 - BBR_RS20465 0.47 -1.4 2,418,377 + BBR_RS20465 0.54 -0.4 2,418,447 - BBR_RS20465 0.61 +0.6 2,418,447 - BBR_RS20465 0.61 -1.1 2,418,547 - BBR_RS20465 0.72 -1.7 2,418,556 - BBR_RS20465 0.73 -2.0 2,418,579 - BBR_RS20465 0.75 +1.5 2,418,579 - BBR_RS20465 0.75 -0.7 2,418,584 - BBR_RS20465 0.75 +0.1 2,418,607 - BBR_RS20465 0.78 -1.8 2,418,624 - BBR_RS20465 0.80 -1.7 2,418,657 + BBR_RS20465 0.83 -0.3 2,418,659 - BBR_RS20465 0.83 -2.2 2,418,659 - BBR_RS20465 0.83 +0.5 2,418,664 + BBR_RS20465 0.84 -1.4 2,418,672 - BBR_RS20465 0.85 -0.2 2,418,727 + -0.1 2,418,727 + +0.6 2,418,727 + -1.0 2,418,735 - -2.0 2,418,735 - -0.1 2,418,745 - -1.6 2,418,745 - -2.5 2,418,798 + -0.2 2,418,817 + +2.1 2,418,822 - -1.4 2,418,828 + +0.0 2,418,836 - -1.3 2,418,844 + -1.0 2,418,846 - -0.8 2,418,850 + +0.3 2,418,852 + +0.8 2,418,852 + -0.4 2,418,852 - -0.7 2,418,852 - -0.1
Or see this region's nucleotide sequence