Experiment: Recovery broth with Lacidipine 0.4 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS19165 and BBR_RS19170 are separated by 253 nucleotides BBR_RS19170 and BBR_RS19175 are separated by 118 nucleotides
BBR_RS19165: BBR_RS19165 - phosphate ABC transporter substrate-binding protein PstS, at 2,074,194 to 2,075,324
_RS19165
BBR_RS19170: BBR_RS19170 - DNA-binding response regulator, at 2,075,578 to 2,076,282
_RS19170
BBR_RS19175: BBR_RS19175 - two-component sensor histidine kinase, at 2,076,401 to 2,077,696
_RS19175
Position (kb)
2075
2076
2077 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2074.632 kb on - strand, within BBR_RS19165 at 2075.371 kb on - strand at 2075.381 kb on + strand at 2075.392 kb on + strand at 2075.408 kb on + strand at 2075.445 kb on + strand at 2075.445 kb on + strand at 2075.453 kb on + strand at 2075.460 kb on - strand at 2075.490 kb on - strand at 2075.496 kb on + strand at 2075.497 kb on + strand at 2075.497 kb on + strand at 2075.497 kb on + strand at 2075.497 kb on + strand at 2075.497 kb on + strand at 2075.504 kb on - strand at 2075.504 kb on - strand at 2075.505 kb on - strand at 2075.505 kb on - strand at 2075.513 kb on - strand at 2075.513 kb on - strand at 2075.604 kb on + strand at 2075.612 kb on + strand at 2075.612 kb on - strand at 2075.612 kb on - strand at 2075.621 kb on + strand at 2075.623 kb on + strand at 2075.623 kb on + strand at 2075.623 kb on + strand at 2075.631 kb on - strand at 2075.632 kb on + strand at 2075.632 kb on - strand at 2075.638 kb on - strand at 2075.638 kb on - strand at 2075.638 kb on - strand at 2075.638 kb on - strand at 2075.638 kb on - strand at 2075.640 kb on + strand at 2075.640 kb on + strand at 2075.640 kb on - strand at 2075.640 kb on - strand at 2075.672 kb on + strand, within BBR_RS19170 at 2075.672 kb on + strand, within BBR_RS19170 at 2075.672 kb on + strand, within BBR_RS19170 at 2075.672 kb on + strand, within BBR_RS19170 at 2075.672 kb on + strand, within BBR_RS19170 at 2075.680 kb on - strand, within BBR_RS19170 at 2075.680 kb on - strand, within BBR_RS19170 at 2075.680 kb on - strand, within BBR_RS19170 at 2075.680 kb on - strand, within BBR_RS19170 at 2075.680 kb on - strand, within BBR_RS19170 at 2075.680 kb on - strand, within BBR_RS19170 at 2075.680 kb on - strand, within BBR_RS19170 at 2075.680 kb on - strand, within BBR_RS19170 at 2075.680 kb on - strand, within BBR_RS19170 at 2075.685 kb on + strand, within BBR_RS19170 at 2075.685 kb on + strand, within BBR_RS19170 at 2075.685 kb on + strand, within BBR_RS19170 at 2075.685 kb on + strand, within BBR_RS19170 at 2075.691 kb on - strand, within BBR_RS19170 at 2075.693 kb on - strand, within BBR_RS19170 at 2075.693 kb on - strand, within BBR_RS19170 at 2075.762 kb on + strand, within BBR_RS19170 at 2075.764 kb on - strand, within BBR_RS19170 at 2075.770 kb on - strand, within BBR_RS19170 at 2075.770 kb on - strand, within BBR_RS19170 at 2075.788 kb on - strand, within BBR_RS19170 at 2075.812 kb on + strand, within BBR_RS19170 at 2075.832 kb on + strand, within BBR_RS19170 at 2075.832 kb on + strand, within BBR_RS19170 at 2075.832 kb on + strand, within BBR_RS19170 at 2075.849 kb on - strand, within BBR_RS19170 at 2075.849 kb on - strand, within BBR_RS19170 at 2075.862 kb on - strand, within BBR_RS19170 at 2075.890 kb on + strand, within BBR_RS19170 at 2075.903 kb on + strand, within BBR_RS19170 at 2075.903 kb on - strand, within BBR_RS19170 at 2075.908 kb on + strand, within BBR_RS19170 at 2075.911 kb on - strand, within BBR_RS19170 at 2075.948 kb on - strand, within BBR_RS19170 at 2076.014 kb on + strand, within BBR_RS19170 at 2076.014 kb on + strand, within BBR_RS19170 at 2076.015 kb on - strand, within BBR_RS19170 at 2076.021 kb on - strand, within BBR_RS19170 at 2076.021 kb on - strand, within BBR_RS19170 at 2076.021 kb on - strand, within BBR_RS19170 at 2076.022 kb on - strand, within BBR_RS19170 at 2076.022 kb on - strand, within BBR_RS19170 at 2076.022 kb on - strand, within BBR_RS19170 at 2076.022 kb on - strand, within BBR_RS19170 at 2076.057 kb on - strand, within BBR_RS19170 at 2076.071 kb on - strand, within BBR_RS19170 at 2076.078 kb on - strand, within BBR_RS19170 at 2076.096 kb on + strand, within BBR_RS19170 at 2076.096 kb on + strand, within BBR_RS19170 at 2076.096 kb on + strand, within BBR_RS19170 at 2076.099 kb on - strand, within BBR_RS19170 at 2076.099 kb on - strand, within BBR_RS19170 at 2076.104 kb on - strand, within BBR_RS19170 at 2076.109 kb on - strand, within BBR_RS19170 at 2076.133 kb on - strand, within BBR_RS19170 at 2076.135 kb on + strand, within BBR_RS19170 at 2076.136 kb on + strand, within BBR_RS19170 at 2076.138 kb on - strand, within BBR_RS19170 at 2076.141 kb on + strand, within BBR_RS19170 at 2076.149 kb on - strand, within BBR_RS19170 at 2076.150 kb on + strand, within BBR_RS19170 at 2076.158 kb on - strand, within BBR_RS19170 at 2076.158 kb on - strand, within BBR_RS19170 at 2076.165 kb on + strand, within BBR_RS19170 at 2076.166 kb on + strand, within BBR_RS19170 at 2076.173 kb on - strand, within BBR_RS19170 at 2076.173 kb on - strand, within BBR_RS19170 at 2076.173 kb on - strand, within BBR_RS19170 at 2076.173 kb on - strand, within BBR_RS19170 at 2076.173 kb on - strand, within BBR_RS19170 at 2076.173 kb on - strand, within BBR_RS19170 at 2076.173 kb on - strand, within BBR_RS19170 at 2076.173 kb on - strand, within BBR_RS19170 at 2076.173 kb on - strand, within BBR_RS19170 at 2076.173 kb on - strand, within BBR_RS19170 at 2076.174 kb on - strand, within BBR_RS19170 at 2076.201 kb on + strand, within BBR_RS19170 at 2076.201 kb on + strand, within BBR_RS19170 at 2076.202 kb on + strand, within BBR_RS19170 at 2076.202 kb on + strand, within BBR_RS19170 at 2076.202 kb on + strand, within BBR_RS19170 at 2076.202 kb on + strand, within BBR_RS19170 at 2076.202 kb on + strand, within BBR_RS19170 at 2076.209 kb on - strand, within BBR_RS19170 at 2076.209 kb on - strand, within BBR_RS19170 at 2076.209 kb on - strand, within BBR_RS19170 at 2076.209 kb on - strand, within BBR_RS19170 at 2076.209 kb on - strand, within BBR_RS19170 at 2076.209 kb on - strand, within BBR_RS19170 at 2076.209 kb on - strand, within BBR_RS19170 at 2076.210 kb on - strand, within BBR_RS19170 at 2076.210 kb on - strand, within BBR_RS19170 at 2076.210 kb on - strand, within BBR_RS19170 at 2076.210 kb on - strand, within BBR_RS19170 at 2076.218 kb on - strand at 2076.223 kb on - strand at 2076.223 kb on - strand at 2076.229 kb on + strand at 2076.302 kb on + strand at 2076.326 kb on + strand at 2076.356 kb on + strand at 2076.356 kb on + strand at 2076.356 kb on + strand at 2076.356 kb on + strand at 2076.356 kb on + strand at 2076.356 kb on + strand at 2076.364 kb on - strand at 2076.364 kb on - strand at 2076.369 kb on - strand at 2076.374 kb on - strand at 2076.380 kb on + strand at 2076.407 kb on + strand at 2076.417 kb on + strand at 2076.417 kb on + strand at 2076.417 kb on + strand at 2076.417 kb on + strand at 2076.424 kb on + strand at 2076.425 kb on + strand at 2076.428 kb on - strand at 2076.433 kb on + strand at 2076.433 kb on - strand at 2076.433 kb on - strand at 2076.441 kb on - strand at 2076.447 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Lacidipine 0.4 mM remove 2,074,632 - BBR_RS19165 0.39 -1.0 2,075,371 - +0.1 2,075,381 + +0.6 2,075,392 + +0.3 2,075,408 + -0.5 2,075,445 + +0.2 2,075,445 + -0.0 2,075,453 + +0.3 2,075,460 - +0.2 2,075,490 - -0.1 2,075,496 + +0.4 2,075,497 + -0.2 2,075,497 + -0.8 2,075,497 + +0.6 2,075,497 + +1.1 2,075,497 + +0.8 2,075,504 - +0.6 2,075,504 - +0.0 2,075,505 - -0.5 2,075,505 - +0.1 2,075,513 - -1.8 2,075,513 - +1.0 2,075,604 + -1.0 2,075,612 + -0.5 2,075,612 - -2.2 2,075,612 - -2.0 2,075,621 + -0.4 2,075,623 + -1.4 2,075,623 + -1.7 2,075,623 + -1.4 2,075,631 - -1.4 2,075,632 + -1.2 2,075,632 - -1.3 2,075,638 - -1.8 2,075,638 - -0.4 2,075,638 - -1.2 2,075,638 - -1.5 2,075,638 - -0.9 2,075,640 + -0.5 2,075,640 + -0.4 2,075,640 - -0.6 2,075,640 - -0.2 2,075,672 + BBR_RS19170 0.13 -2.7 2,075,672 + BBR_RS19170 0.13 +0.7 2,075,672 + BBR_RS19170 0.13 +0.1 2,075,672 + BBR_RS19170 0.13 -0.7 2,075,672 + BBR_RS19170 0.13 -1.3 2,075,680 - BBR_RS19170 0.14 -0.6 2,075,680 - BBR_RS19170 0.14 -1.0 2,075,680 - BBR_RS19170 0.14 -1.5 2,075,680 - BBR_RS19170 0.14 -2.4 2,075,680 - BBR_RS19170 0.14 -0.9 2,075,680 - BBR_RS19170 0.14 -0.7 2,075,680 - BBR_RS19170 0.14 -1.4 2,075,680 - BBR_RS19170 0.14 -0.5 2,075,680 - BBR_RS19170 0.14 -1.3 2,075,685 + BBR_RS19170 0.15 -1.4 2,075,685 + BBR_RS19170 0.15 -0.8 2,075,685 + BBR_RS19170 0.15 +0.1 2,075,685 + BBR_RS19170 0.15 -0.3 2,075,691 - BBR_RS19170 0.16 -0.8 2,075,693 - BBR_RS19170 0.16 -2.1 2,075,693 - BBR_RS19170 0.16 -0.7 2,075,762 + BBR_RS19170 0.26 -1.4 2,075,764 - BBR_RS19170 0.26 -0.7 2,075,770 - BBR_RS19170 0.27 -1.7 2,075,770 - BBR_RS19170 0.27 -0.2 2,075,788 - BBR_RS19170 0.30 -0.5 2,075,812 + BBR_RS19170 0.33 -0.3 2,075,832 + BBR_RS19170 0.36 -1.7 2,075,832 + BBR_RS19170 0.36 -1.4 2,075,832 + BBR_RS19170 0.36 -0.9 2,075,849 - BBR_RS19170 0.38 -1.9 2,075,849 - BBR_RS19170 0.38 -0.5 2,075,862 - BBR_RS19170 0.40 -0.4 2,075,890 + BBR_RS19170 0.44 -1.5 2,075,903 + BBR_RS19170 0.46 -1.1 2,075,903 - BBR_RS19170 0.46 -1.2 2,075,908 + BBR_RS19170 0.47 -1.1 2,075,911 - BBR_RS19170 0.47 -1.1 2,075,948 - BBR_RS19170 0.52 -0.6 2,076,014 + BBR_RS19170 0.62 -1.6 2,076,014 + BBR_RS19170 0.62 -1.0 2,076,015 - BBR_RS19170 0.62 -0.8 2,076,021 - BBR_RS19170 0.63 -2.0 2,076,021 - BBR_RS19170 0.63 -1.9 2,076,021 - BBR_RS19170 0.63 -0.2 2,076,022 - BBR_RS19170 0.63 -1.6 2,076,022 - BBR_RS19170 0.63 -0.4 2,076,022 - BBR_RS19170 0.63 -1.2 2,076,022 - BBR_RS19170 0.63 -2.2 2,076,057 - BBR_RS19170 0.68 -0.3 2,076,071 - BBR_RS19170 0.70 -0.8 2,076,078 - BBR_RS19170 0.71 -0.2 2,076,096 + BBR_RS19170 0.73 -0.6 2,076,096 + BBR_RS19170 0.73 -1.0 2,076,096 + BBR_RS19170 0.73 -1.8 2,076,099 - BBR_RS19170 0.74 -0.5 2,076,099 - BBR_RS19170 0.74 +0.5 2,076,104 - BBR_RS19170 0.75 -2.3 2,076,109 - BBR_RS19170 0.75 -2.0 2,076,133 - BBR_RS19170 0.79 -2.8 2,076,135 + BBR_RS19170 0.79 -1.2 2,076,136 + BBR_RS19170 0.79 -1.6 2,076,138 - BBR_RS19170 0.79 -0.9 2,076,141 + BBR_RS19170 0.80 -0.5 2,076,149 - BBR_RS19170 0.81 -2.4 2,076,150 + BBR_RS19170 0.81 -0.5 2,076,158 - BBR_RS19170 0.82 -1.7 2,076,158 - BBR_RS19170 0.82 -1.2 2,076,165 + BBR_RS19170 0.83 -0.5 2,076,166 + BBR_RS19170 0.83 -1.6 2,076,173 - BBR_RS19170 0.84 -1.7 2,076,173 - BBR_RS19170 0.84 -1.0 2,076,173 - BBR_RS19170 0.84 -2.0 2,076,173 - BBR_RS19170 0.84 -1.7 2,076,173 - BBR_RS19170 0.84 -1.6 2,076,173 - BBR_RS19170 0.84 -0.4 2,076,173 - BBR_RS19170 0.84 -1.5 2,076,173 - BBR_RS19170 0.84 -1.3 2,076,173 - BBR_RS19170 0.84 -0.4 2,076,173 - BBR_RS19170 0.84 -0.6 2,076,174 - BBR_RS19170 0.85 -1.4 2,076,201 + BBR_RS19170 0.88 -2.0 2,076,201 + BBR_RS19170 0.88 -0.8 2,076,202 + BBR_RS19170 0.89 -0.6 2,076,202 + BBR_RS19170 0.89 -0.6 2,076,202 + BBR_RS19170 0.89 -2.2 2,076,202 + BBR_RS19170 0.89 -0.6 2,076,202 + BBR_RS19170 0.89 +0.1 2,076,209 - BBR_RS19170 0.90 -3.1 2,076,209 - BBR_RS19170 0.90 -0.5 2,076,209 - BBR_RS19170 0.90 -1.0 2,076,209 - BBR_RS19170 0.90 -1.1 2,076,209 - BBR_RS19170 0.90 -0.8 2,076,209 - BBR_RS19170 0.90 -1.1 2,076,209 - BBR_RS19170 0.90 -1.7 2,076,210 - BBR_RS19170 0.90 -2.3 2,076,210 - BBR_RS19170 0.90 -0.8 2,076,210 - BBR_RS19170 0.90 -1.9 2,076,210 - BBR_RS19170 0.90 -1.3 2,076,218 - -1.5 2,076,223 - -0.9 2,076,223 - -0.9 2,076,229 + -1.2 2,076,302 + -0.9 2,076,326 + +0.3 2,076,356 + -0.5 2,076,356 + +0.4 2,076,356 + -0.0 2,076,356 + +0.4 2,076,356 + +0.2 2,076,356 + +0.1 2,076,364 - +0.3 2,076,364 - -0.2 2,076,369 - +0.1 2,076,374 - -0.5 2,076,380 + -0.6 2,076,407 + +0.9 2,076,417 + -0.2 2,076,417 + +0.2 2,076,417 + +0.5 2,076,417 + -0.3 2,076,424 + +1.2 2,076,425 + +0.7 2,076,428 - +0.6 2,076,433 + +0.7 2,076,433 - -0.4 2,076,433 - +0.7 2,076,441 - -0.5 2,076,447 - +0.4
Or see this region's nucleotide sequence