Experiment: Recovery broth with Lacidipine 0.4 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS18685 and BBR_RS18690 are separated by 239 nucleotides BBR_RS18690 and BBR_RS18695 are separated by 1 nucleotides BBR_RS18695 and BBR_RS18700 are separated by 51 nucleotides
BBR_RS18685: BBR_RS18685 - serine--tRNA ligase, at 1,979,683 to 1,980,969
_RS18685
BBR_RS18690: BBR_RS18690 - diacylglycerol kinase, at 1,981,209 to 1,982,390
_RS18690
BBR_RS18695: BBR_RS18695 - PRD domain-containing protein, at 1,982,392 to 1,983,285
_RS18695
BBR_RS18700: BBR_RS18700 - PTS beta-glucoside transporter subunit EIIBCA, at 1,983,337 to 1,985,427
_RS18700
Position (kb)
1981
1982
1983 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1981.021 kb on + strand at 1981.026 kb on + strand at 1981.029 kb on - strand at 1981.119 kb on + strand at 1981.133 kb on - strand at 1981.133 kb on - strand at 1981.160 kb on - strand at 1981.163 kb on + strand at 1981.184 kb on - strand at 1981.366 kb on - strand, within BBR_RS18690 at 1981.394 kb on - strand, within BBR_RS18690 at 1981.467 kb on + strand, within BBR_RS18690 at 1981.467 kb on + strand, within BBR_RS18690 at 1981.478 kb on + strand, within BBR_RS18690 at 1981.478 kb on + strand, within BBR_RS18690 at 1981.506 kb on + strand, within BBR_RS18690 at 1981.516 kb on + strand, within BBR_RS18690 at 1981.524 kb on - strand, within BBR_RS18690 at 1981.593 kb on + strand, within BBR_RS18690 at 1981.593 kb on + strand, within BBR_RS18690 at 1981.606 kb on - strand, within BBR_RS18690 at 1981.671 kb on + strand, within BBR_RS18690 at 1981.678 kb on - strand, within BBR_RS18690 at 1981.679 kb on - strand, within BBR_RS18690 at 1981.707 kb on + strand, within BBR_RS18690 at 1981.823 kb on + strand, within BBR_RS18690 at 1981.924 kb on + strand, within BBR_RS18690 at 1981.924 kb on + strand, within BBR_RS18690 at 1981.927 kb on + strand, within BBR_RS18690 at 1981.929 kb on + strand, within BBR_RS18690 at 1981.932 kb on - strand, within BBR_RS18690 at 1982.077 kb on + strand, within BBR_RS18690 at 1982.085 kb on - strand, within BBR_RS18690 at 1982.130 kb on - strand, within BBR_RS18690 at 1982.135 kb on - strand, within BBR_RS18690 at 1982.165 kb on + strand, within BBR_RS18690 at 1982.165 kb on + strand, within BBR_RS18690 at 1982.166 kb on + strand, within BBR_RS18690 at 1982.166 kb on + strand, within BBR_RS18690 at 1982.172 kb on + strand, within BBR_RS18690 at 1982.174 kb on - strand, within BBR_RS18690 at 1982.210 kb on + strand, within BBR_RS18690 at 1982.210 kb on + strand, within BBR_RS18690 at 1982.212 kb on - strand, within BBR_RS18690 at 1982.216 kb on + strand, within BBR_RS18690 at 1982.218 kb on - strand, within BBR_RS18690 at 1982.263 kb on - strand, within BBR_RS18690 at 1982.309 kb on + strand at 1982.317 kb on - strand at 1982.317 kb on - strand at 1982.331 kb on + strand at 1982.336 kb on + strand at 1982.452 kb on + strand at 1982.589 kb on - strand, within BBR_RS18695 at 1982.615 kb on + strand, within BBR_RS18695 at 1982.623 kb on - strand, within BBR_RS18695 at 1982.828 kb on + strand, within BBR_RS18695 at 1982.838 kb on + strand, within BBR_RS18695 at 1982.882 kb on + strand, within BBR_RS18695 at 1982.911 kb on - strand, within BBR_RS18695 at 1982.981 kb on - strand, within BBR_RS18695 at 1983.030 kb on + strand, within BBR_RS18695 at 1983.130 kb on + strand, within BBR_RS18695 at 1983.131 kb on - strand, within BBR_RS18695 at 1983.138 kb on - strand, within BBR_RS18695 at 1983.143 kb on - strand, within BBR_RS18695 at 1983.191 kb on + strand, within BBR_RS18695 at 1983.327 kb on - strand at 1983.327 kb on - strand at 1983.335 kb on - strand at 1983.338 kb on - strand at 1983.357 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Lacidipine 0.4 mM remove 1,981,021 + +0.0 1,981,026 + -0.8 1,981,029 - -1.1 1,981,119 + +0.0 1,981,133 - -0.1 1,981,133 - -0.7 1,981,160 - -0.1 1,981,163 + +0.4 1,981,184 - -0.6 1,981,366 - BBR_RS18690 0.13 +1.1 1,981,394 - BBR_RS18690 0.16 -3.1 1,981,467 + BBR_RS18690 0.22 -1.8 1,981,467 + BBR_RS18690 0.22 -0.8 1,981,478 + BBR_RS18690 0.23 -0.8 1,981,478 + BBR_RS18690 0.23 -2.7 1,981,506 + BBR_RS18690 0.25 +1.8 1,981,516 + BBR_RS18690 0.26 -1.9 1,981,524 - BBR_RS18690 0.27 +0.2 1,981,593 + BBR_RS18690 0.32 -1.2 1,981,593 + BBR_RS18690 0.32 +0.5 1,981,606 - BBR_RS18690 0.34 -2.3 1,981,671 + BBR_RS18690 0.39 -0.5 1,981,678 - BBR_RS18690 0.40 -1.1 1,981,679 - BBR_RS18690 0.40 -0.2 1,981,707 + BBR_RS18690 0.42 -1.3 1,981,823 + BBR_RS18690 0.52 -0.9 1,981,924 + BBR_RS18690 0.60 -0.2 1,981,924 + BBR_RS18690 0.60 +1.1 1,981,927 + BBR_RS18690 0.61 -2.1 1,981,929 + BBR_RS18690 0.61 -0.3 1,981,932 - BBR_RS18690 0.61 -1.3 1,982,077 + BBR_RS18690 0.73 -2.6 1,982,085 - BBR_RS18690 0.74 -1.6 1,982,130 - BBR_RS18690 0.78 -0.8 1,982,135 - BBR_RS18690 0.78 -2.5 1,982,165 + BBR_RS18690 0.81 -0.6 1,982,165 + BBR_RS18690 0.81 -0.1 1,982,166 + BBR_RS18690 0.81 -1.6 1,982,166 + BBR_RS18690 0.81 -1.7 1,982,172 + BBR_RS18690 0.81 -0.7 1,982,174 - BBR_RS18690 0.82 -1.1 1,982,210 + BBR_RS18690 0.85 -1.4 1,982,210 + BBR_RS18690 0.85 -1.5 1,982,212 - BBR_RS18690 0.85 +0.5 1,982,216 + BBR_RS18690 0.85 -0.1 1,982,218 - BBR_RS18690 0.85 -2.0 1,982,263 - BBR_RS18690 0.89 -0.1 1,982,309 + -0.6 1,982,317 - -1.0 1,982,317 - +0.1 1,982,331 + -0.3 1,982,336 + -0.1 1,982,452 + -0.7 1,982,589 - BBR_RS18695 0.22 -0.3 1,982,615 + BBR_RS18695 0.25 -0.1 1,982,623 - BBR_RS18695 0.26 +0.0 1,982,828 + BBR_RS18695 0.49 -1.4 1,982,838 + BBR_RS18695 0.50 -1.1 1,982,882 + BBR_RS18695 0.55 +0.1 1,982,911 - BBR_RS18695 0.58 -0.2 1,982,981 - BBR_RS18695 0.66 +0.3 1,983,030 + BBR_RS18695 0.71 -1.1 1,983,130 + BBR_RS18695 0.83 -0.9 1,983,131 - BBR_RS18695 0.83 -1.1 1,983,138 - BBR_RS18695 0.83 +0.1 1,983,143 - BBR_RS18695 0.84 -0.5 1,983,191 + BBR_RS18695 0.89 -0.8 1,983,327 - -1.5 1,983,327 - +0.3 1,983,335 - +0.0 1,983,338 - -0.3 1,983,357 - +0.4
Or see this region's nucleotide sequence