Experiment: Recovery broth with Lacidipine 0.4 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS18555 and BBR_RS18560 are separated by 159 nucleotides BBR_RS18560 and BBR_RS18565 are separated by 101 nucleotides BBR_RS18565 and BBR_RS18570 are separated by 253 nucleotides
BBR_RS18555: BBR_RS18555 - NlpC/P60 family protein, at 1,948,821 to 1,949,546
_RS18555
BBR_RS18560: BBR_RS18560 - hypothetical protein, at 1,949,706 to 1,950,443
_RS18560
BBR_RS18565: BBR_RS18565 - CHAP domain-containing protein, at 1,950,545 to 1,951,504
_RS18565
BBR_RS18570: BBR_RS18570 - phosphoserine transaminase, at 1,951,758 to 1,952,894
_RS18570
Position (kb)
1950
1951
1952 Strain fitness (log2 ratio)
-2
-1
0
1 at 1949.549 kb on + strand at 1949.549 kb on + strand at 1949.586 kb on + strand at 1949.586 kb on + strand at 1949.594 kb on - strand at 1949.594 kb on - strand at 1949.645 kb on + strand at 1949.653 kb on - strand at 1949.696 kb on + strand at 1949.696 kb on + strand at 1949.705 kb on - strand at 1949.728 kb on - strand at 1949.772 kb on + strand at 1949.780 kb on - strand, within BBR_RS18560 at 1949.780 kb on - strand, within BBR_RS18560 at 1949.780 kb on - strand, within BBR_RS18560 at 1949.886 kb on - strand, within BBR_RS18560 at 1949.886 kb on - strand, within BBR_RS18560 at 1949.901 kb on - strand, within BBR_RS18560 at 1949.911 kb on - strand, within BBR_RS18560 at 1949.911 kb on - strand, within BBR_RS18560 at 1949.923 kb on + strand, within BBR_RS18560 at 1949.941 kb on - strand, within BBR_RS18560 at 1949.951 kb on + strand, within BBR_RS18560 at 1949.982 kb on + strand, within BBR_RS18560 at 1949.982 kb on + strand, within BBR_RS18560 at 1949.982 kb on + strand, within BBR_RS18560 at 1949.982 kb on - strand, within BBR_RS18560 at 1949.984 kb on + strand, within BBR_RS18560 at 1949.985 kb on - strand, within BBR_RS18560 at 1949.985 kb on - strand, within BBR_RS18560 at 1949.989 kb on + strand, within BBR_RS18560 at 1949.990 kb on - strand, within BBR_RS18560 at 1949.990 kb on - strand, within BBR_RS18560 at 1949.990 kb on - strand, within BBR_RS18560 at 1949.990 kb on - strand, within BBR_RS18560 at 1949.999 kb on + strand, within BBR_RS18560 at 1950.005 kb on - strand, within BBR_RS18560 at 1950.007 kb on - strand, within BBR_RS18560 at 1950.059 kb on + strand, within BBR_RS18560 at 1950.076 kb on + strand, within BBR_RS18560 at 1950.077 kb on - strand, within BBR_RS18560 at 1950.099 kb on - strand, within BBR_RS18560 at 1950.109 kb on - strand, within BBR_RS18560 at 1950.121 kb on + strand, within BBR_RS18560 at 1950.126 kb on + strand, within BBR_RS18560 at 1950.143 kb on - strand, within BBR_RS18560 at 1950.147 kb on + strand, within BBR_RS18560 at 1950.163 kb on - strand, within BBR_RS18560 at 1950.175 kb on - strand, within BBR_RS18560 at 1950.205 kb on + strand, within BBR_RS18560 at 1950.205 kb on + strand, within BBR_RS18560 at 1950.227 kb on + strand, within BBR_RS18560 at 1950.235 kb on - strand, within BBR_RS18560 at 1950.242 kb on + strand, within BBR_RS18560 at 1950.263 kb on + strand, within BBR_RS18560 at 1950.296 kb on + strand, within BBR_RS18560 at 1950.304 kb on - strand, within BBR_RS18560 at 1950.304 kb on - strand, within BBR_RS18560 at 1950.304 kb on - strand, within BBR_RS18560 at 1950.304 kb on - strand, within BBR_RS18560 at 1950.329 kb on + strand, within BBR_RS18560 at 1950.332 kb on + strand, within BBR_RS18560 at 1950.332 kb on + strand, within BBR_RS18560 at 1950.340 kb on - strand, within BBR_RS18560 at 1950.341 kb on + strand, within BBR_RS18560 at 1950.347 kb on - strand, within BBR_RS18560 at 1950.348 kb on - strand, within BBR_RS18560 at 1950.348 kb on - strand, within BBR_RS18560 at 1950.351 kb on - strand, within BBR_RS18560 at 1950.453 kb on + strand at 1950.461 kb on - strand at 1950.461 kb on - strand at 1950.461 kb on - strand at 1950.462 kb on - strand at 1950.509 kb on - strand at 1950.871 kb on - strand, within BBR_RS18565 at 1950.881 kb on - strand, within BBR_RS18565 at 1951.137 kb on + strand, within BBR_RS18565 at 1951.475 kb on - strand at 1951.589 kb on - strand at 1951.591 kb on + strand at 1951.619 kb on - strand at 1951.725 kb on - strand at 1951.727 kb on + strand at 1951.737 kb on + strand at 1951.742 kb on + strand at 1951.748 kb on - strand at 1951.748 kb on - strand at 1951.781 kb on + strand at 1951.781 kb on + strand at 1951.782 kb on + strand at 1951.807 kb on + strand at 1951.807 kb on + strand at 1951.815 kb on - strand at 1951.841 kb on - strand at 1951.856 kb on - strand at 1951.871 kb on + strand at 1951.871 kb on + strand at 1951.871 kb on + strand at 1951.871 kb on + strand at 1951.876 kb on + strand, within BBR_RS18570 at 1951.879 kb on - strand, within BBR_RS18570 at 1951.879 kb on - strand, within BBR_RS18570 at 1951.879 kb on - strand, within BBR_RS18570 at 1951.879 kb on - strand, within BBR_RS18570 at 1951.913 kb on - strand, within BBR_RS18570 at 1951.971 kb on + strand, within BBR_RS18570 at 1951.976 kb on + strand, within BBR_RS18570 at 1951.983 kb on + strand, within BBR_RS18570 at 1952.027 kb on - strand, within BBR_RS18570 at 1952.054 kb on + strand, within BBR_RS18570 at 1952.073 kb on - strand, within BBR_RS18570 at 1952.090 kb on - strand, within BBR_RS18570 at 1952.094 kb on + strand, within BBR_RS18570 at 1952.101 kb on + strand, within BBR_RS18570 at 1952.101 kb on + strand, within BBR_RS18570 at 1952.123 kb on - strand, within BBR_RS18570 at 1952.132 kb on + strand, within BBR_RS18570 at 1952.132 kb on + strand, within BBR_RS18570 at 1952.159 kb on + strand, within BBR_RS18570 at 1952.159 kb on + strand, within BBR_RS18570 at 1952.159 kb on + strand, within BBR_RS18570 at 1952.159 kb on + strand, within BBR_RS18570 at 1952.167 kb on + strand, within BBR_RS18570 at 1952.174 kb on + strand, within BBR_RS18570 at 1952.177 kb on - strand, within BBR_RS18570 at 1952.198 kb on + strand, within BBR_RS18570 at 1952.198 kb on + strand, within BBR_RS18570 at 1952.200 kb on - strand, within BBR_RS18570 at 1952.203 kb on + strand, within BBR_RS18570 at 1952.242 kb on + strand, within BBR_RS18570 at 1952.242 kb on + strand, within BBR_RS18570 at 1952.273 kb on - strand, within BBR_RS18570 at 1952.301 kb on + strand, within BBR_RS18570 at 1952.305 kb on + strand, within BBR_RS18570 at 1952.305 kb on + strand, within BBR_RS18570 at 1952.322 kb on + strand, within BBR_RS18570 at 1952.330 kb on - strand, within BBR_RS18570 at 1952.339 kb on + strand, within BBR_RS18570 at 1952.339 kb on + strand, within BBR_RS18570 at 1952.367 kb on + strand, within BBR_RS18570 at 1952.369 kb on + strand, within BBR_RS18570 at 1952.371 kb on + strand, within BBR_RS18570 at 1952.377 kb on - strand, within BBR_RS18570 at 1952.377 kb on - strand, within BBR_RS18570 at 1952.377 kb on - strand, within BBR_RS18570 at 1952.379 kb on - strand, within BBR_RS18570 at 1952.394 kb on + strand, within BBR_RS18570 at 1952.394 kb on + strand, within BBR_RS18570 at 1952.394 kb on + strand, within BBR_RS18570 at 1952.394 kb on - strand, within BBR_RS18570 at 1952.402 kb on - strand, within BBR_RS18570 at 1952.412 kb on + strand, within BBR_RS18570 at 1952.412 kb on + strand, within BBR_RS18570 at 1952.413 kb on + strand, within BBR_RS18570 at 1952.413 kb on + strand, within BBR_RS18570 at 1952.415 kb on + strand, within BBR_RS18570 at 1952.420 kb on + strand, within BBR_RS18570 at 1952.420 kb on + strand, within BBR_RS18570 at 1952.432 kb on + strand, within BBR_RS18570 at 1952.432 kb on + strand, within BBR_RS18570 at 1952.432 kb on + strand, within BBR_RS18570 at 1952.432 kb on + strand, within BBR_RS18570 at 1952.432 kb on + strand, within BBR_RS18570 at 1952.432 kb on + strand, within BBR_RS18570 at 1952.440 kb on - strand, within BBR_RS18570 at 1952.440 kb on - strand, within BBR_RS18570 at 1952.462 kb on + strand, within BBR_RS18570 at 1952.468 kb on + strand, within BBR_RS18570 at 1952.469 kb on + strand, within BBR_RS18570 at 1952.477 kb on - strand, within BBR_RS18570 at 1952.492 kb on - strand, within BBR_RS18570 at 1952.501 kb on + strand, within BBR_RS18570
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Lacidipine 0.4 mM remove 1,949,549 + +0.1 1,949,549 + +0.2 1,949,586 + +0.2 1,949,586 + +0.2 1,949,594 - -0.2 1,949,594 - +1.4 1,949,645 + +0.5 1,949,653 - -0.7 1,949,696 + +0.4 1,949,696 + -0.1 1,949,705 - +0.0 1,949,728 - -0.3 1,949,772 + -0.7 1,949,780 - BBR_RS18560 0.10 +0.5 1,949,780 - BBR_RS18560 0.10 -0.6 1,949,780 - BBR_RS18560 0.10 +0.1 1,949,886 - BBR_RS18560 0.24 +1.0 1,949,886 - BBR_RS18560 0.24 -0.4 1,949,901 - BBR_RS18560 0.26 +0.4 1,949,911 - BBR_RS18560 0.28 -0.4 1,949,911 - BBR_RS18560 0.28 -0.3 1,949,923 + BBR_RS18560 0.29 +0.8 1,949,941 - BBR_RS18560 0.32 -0.3 1,949,951 + BBR_RS18560 0.33 -0.5 1,949,982 + BBR_RS18560 0.37 +0.8 1,949,982 + BBR_RS18560 0.37 +0.2 1,949,982 + BBR_RS18560 0.37 +0.8 1,949,982 - BBR_RS18560 0.37 -1.5 1,949,984 + BBR_RS18560 0.38 +0.5 1,949,985 - BBR_RS18560 0.38 +0.9 1,949,985 - BBR_RS18560 0.38 -1.0 1,949,989 + BBR_RS18560 0.38 -0.1 1,949,990 - BBR_RS18560 0.38 -0.5 1,949,990 - BBR_RS18560 0.38 -0.9 1,949,990 - BBR_RS18560 0.38 +0.1 1,949,990 - BBR_RS18560 0.38 -0.5 1,949,999 + BBR_RS18560 0.40 +1.3 1,950,005 - BBR_RS18560 0.41 +0.5 1,950,007 - BBR_RS18560 0.41 -0.0 1,950,059 + BBR_RS18560 0.48 +0.9 1,950,076 + BBR_RS18560 0.50 +0.5 1,950,077 - BBR_RS18560 0.50 +0.2 1,950,099 - BBR_RS18560 0.53 +0.7 1,950,109 - BBR_RS18560 0.55 +0.1 1,950,121 + BBR_RS18560 0.56 +0.6 1,950,126 + BBR_RS18560 0.57 -0.0 1,950,143 - BBR_RS18560 0.59 -0.3 1,950,147 + BBR_RS18560 0.60 +0.4 1,950,163 - BBR_RS18560 0.62 -0.5 1,950,175 - BBR_RS18560 0.64 +0.3 1,950,205 + BBR_RS18560 0.68 +1.0 1,950,205 + BBR_RS18560 0.68 +0.3 1,950,227 + BBR_RS18560 0.71 +1.4 1,950,235 - BBR_RS18560 0.72 +0.9 1,950,242 + BBR_RS18560 0.73 +0.9 1,950,263 + BBR_RS18560 0.75 -0.4 1,950,296 + BBR_RS18560 0.80 -0.4 1,950,304 - BBR_RS18560 0.81 +0.7 1,950,304 - BBR_RS18560 0.81 -1.1 1,950,304 - BBR_RS18560 0.81 -1.0 1,950,304 - BBR_RS18560 0.81 +0.1 1,950,329 + BBR_RS18560 0.84 +0.1 1,950,332 + BBR_RS18560 0.85 +1.1 1,950,332 + BBR_RS18560 0.85 +0.3 1,950,340 - BBR_RS18560 0.86 -0.9 1,950,341 + BBR_RS18560 0.86 +1.3 1,950,347 - BBR_RS18560 0.87 -0.0 1,950,348 - BBR_RS18560 0.87 -0.5 1,950,348 - BBR_RS18560 0.87 -0.9 1,950,351 - BBR_RS18560 0.87 +0.1 1,950,453 + +1.0 1,950,461 - -0.6 1,950,461 - +0.1 1,950,461 - +0.0 1,950,462 - -1.2 1,950,509 - +0.8 1,950,871 - BBR_RS18565 0.34 -0.1 1,950,881 - BBR_RS18565 0.35 -1.3 1,951,137 + BBR_RS18565 0.62 +0.5 1,951,475 - -0.2 1,951,589 - -0.7 1,951,591 + +0.4 1,951,619 - -0.9 1,951,725 - +0.0 1,951,727 + -0.4 1,951,737 + +1.4 1,951,742 + -0.7 1,951,748 - -0.4 1,951,748 - -0.1 1,951,781 + +1.0 1,951,781 + -1.3 1,951,782 + +0.0 1,951,807 + -0.5 1,951,807 + -1.3 1,951,815 - +0.5 1,951,841 - -0.4 1,951,856 - -0.5 1,951,871 + +0.0 1,951,871 + -0.6 1,951,871 + +0.5 1,951,871 + -0.6 1,951,876 + BBR_RS18570 0.10 +0.1 1,951,879 - BBR_RS18570 0.11 -0.6 1,951,879 - BBR_RS18570 0.11 -0.9 1,951,879 - BBR_RS18570 0.11 -0.3 1,951,879 - BBR_RS18570 0.11 -0.5 1,951,913 - BBR_RS18570 0.14 +0.5 1,951,971 + BBR_RS18570 0.19 +1.3 1,951,976 + BBR_RS18570 0.19 -0.9 1,951,983 + BBR_RS18570 0.20 +1.0 1,952,027 - BBR_RS18570 0.24 +1.1 1,952,054 + BBR_RS18570 0.26 -1.0 1,952,073 - BBR_RS18570 0.28 +0.2 1,952,090 - BBR_RS18570 0.29 +0.8 1,952,094 + BBR_RS18570 0.30 -0.3 1,952,101 + BBR_RS18570 0.30 +1.4 1,952,101 + BBR_RS18570 0.30 -0.9 1,952,123 - BBR_RS18570 0.32 +0.6 1,952,132 + BBR_RS18570 0.33 -1.5 1,952,132 + BBR_RS18570 0.33 +0.8 1,952,159 + BBR_RS18570 0.35 +0.0 1,952,159 + BBR_RS18570 0.35 +0.9 1,952,159 + BBR_RS18570 0.35 +0.4 1,952,159 + BBR_RS18570 0.35 -0.6 1,952,167 + BBR_RS18570 0.36 -1.5 1,952,174 + BBR_RS18570 0.37 -0.4 1,952,177 - BBR_RS18570 0.37 +0.7 1,952,198 + BBR_RS18570 0.39 +0.3 1,952,198 + BBR_RS18570 0.39 -0.1 1,952,200 - BBR_RS18570 0.39 +0.1 1,952,203 + BBR_RS18570 0.39 -0.4 1,952,242 + BBR_RS18570 0.43 +0.6 1,952,242 + BBR_RS18570 0.43 +0.2 1,952,273 - BBR_RS18570 0.45 +1.2 1,952,301 + BBR_RS18570 0.48 -0.0 1,952,305 + BBR_RS18570 0.48 -0.5 1,952,305 + BBR_RS18570 0.48 +0.0 1,952,322 + BBR_RS18570 0.50 -0.5 1,952,330 - BBR_RS18570 0.50 +0.9 1,952,339 + BBR_RS18570 0.51 -0.0 1,952,339 + BBR_RS18570 0.51 -1.1 1,952,367 + BBR_RS18570 0.54 +0.7 1,952,369 + BBR_RS18570 0.54 -0.2 1,952,371 + BBR_RS18570 0.54 +0.8 1,952,377 - BBR_RS18570 0.54 -1.3 1,952,377 - BBR_RS18570 0.54 +0.6 1,952,377 - BBR_RS18570 0.54 -0.1 1,952,379 - BBR_RS18570 0.55 -1.6 1,952,394 + BBR_RS18570 0.56 +0.9 1,952,394 + BBR_RS18570 0.56 -0.3 1,952,394 + BBR_RS18570 0.56 +0.3 1,952,394 - BBR_RS18570 0.56 -0.2 1,952,402 - BBR_RS18570 0.57 +0.1 1,952,412 + BBR_RS18570 0.58 +0.4 1,952,412 + BBR_RS18570 0.58 -0.9 1,952,413 + BBR_RS18570 0.58 -0.1 1,952,413 + BBR_RS18570 0.58 -2.5 1,952,415 + BBR_RS18570 0.58 +0.3 1,952,420 + BBR_RS18570 0.58 -0.4 1,952,420 + BBR_RS18570 0.58 -1.6 1,952,432 + BBR_RS18570 0.59 +0.2 1,952,432 + BBR_RS18570 0.59 +0.1 1,952,432 + BBR_RS18570 0.59 +0.8 1,952,432 + BBR_RS18570 0.59 +0.4 1,952,432 + BBR_RS18570 0.59 +0.7 1,952,432 + BBR_RS18570 0.59 +1.0 1,952,440 - BBR_RS18570 0.60 +0.7 1,952,440 - BBR_RS18570 0.60 -1.2 1,952,462 + BBR_RS18570 0.62 +0.3 1,952,468 + BBR_RS18570 0.62 -0.5 1,952,469 + BBR_RS18570 0.63 -1.0 1,952,477 - BBR_RS18570 0.63 -1.1 1,952,492 - BBR_RS18570 0.65 +0.4 1,952,501 + BBR_RS18570 0.65 -0.0
Or see this region's nucleotide sequence