Experiment: Recovery broth with Lacidipine 0.4 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS18170 and BBR_RS18175 are separated by 197 nucleotides BBR_RS18175 and BBR_RS18180 are separated by 135 nucleotides BBR_RS18180 and BBR_RS18185 overlap by 4 nucleotides BBR_RS18185 and BBR_RS18190 overlap by 14 nucleotides
BBR_RS18170: BBR_RS18170 - dihydropteroate synthase, at 1,862,194 to 1,863,069
_RS18170
BBR_RS18175: BBR_RS18175 - GTP cyclohydrolase I FolE, at 1,863,267 to 1,863,887
_RS18175
BBR_RS18180: BBR_RS18180 - ATP-dependent zinc metalloprotease FtsH, at 1,864,023 to 1,866,122
_RS18180
BBR_RS18185: BBR_RS18185 - hypoxanthine phosphoribosyltransferase, at 1,866,119 to 1,866,682
_RS18185
BBR_RS18190: BBR_RS18190 - tRNA lysidine(34) synthetase TilS, at 1,866,669 to 1,867,820
_RS18190
Position (kb)
1864
1865
1866
1867 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1863.093 kb on + strand at 1863.098 kb on - strand at 1863.108 kb on + strand at 1863.116 kb on - strand at 1863.116 kb on - strand at 1863.116 kb on - strand at 1863.122 kb on - strand at 1863.126 kb on - strand at 1863.127 kb on - strand at 1863.198 kb on + strand at 1863.211 kb on - strand at 1863.211 kb on - strand at 1863.220 kb on + strand at 1863.228 kb on - strand at 1863.236 kb on - strand at 1863.251 kb on + strand at 1863.310 kb on + strand at 1863.464 kb on - strand, within BBR_RS18175 at 1863.489 kb on - strand, within BBR_RS18175 at 1863.975 kb on + strand at 1864.012 kb on - strand at 1864.068 kb on + strand at 1864.090 kb on - strand at 1864.118 kb on + strand at 1864.136 kb on - strand at 1864.136 kb on - strand at 1864.146 kb on - strand at 1864.163 kb on - strand at 1864.194 kb on - strand at 1864.286 kb on - strand, within BBR_RS18180 at 1864.339 kb on - strand, within BBR_RS18180 at 1864.339 kb on - strand, within BBR_RS18180 at 1864.339 kb on - strand, within BBR_RS18180 at 1864.361 kb on - strand, within BBR_RS18180 at 1864.372 kb on + strand, within BBR_RS18180 at 1864.380 kb on - strand, within BBR_RS18180 at 1864.499 kb on - strand, within BBR_RS18180 at 1864.499 kb on - strand, within BBR_RS18180 at 1864.570 kb on - strand, within BBR_RS18180 at 1864.575 kb on - strand, within BBR_RS18180 at 1864.593 kb on - strand, within BBR_RS18180 at 1864.593 kb on - strand, within BBR_RS18180 at 1864.647 kb on - strand, within BBR_RS18180 at 1864.667 kb on - strand, within BBR_RS18180 at 1864.683 kb on - strand, within BBR_RS18180 at 1864.683 kb on - strand, within BBR_RS18180 at 1864.731 kb on - strand, within BBR_RS18180 at 1864.771 kb on - strand, within BBR_RS18180 at 1864.795 kb on - strand, within BBR_RS18180 at 1864.817 kb on - strand, within BBR_RS18180 at 1864.850 kb on - strand, within BBR_RS18180 at 1864.856 kb on + strand, within BBR_RS18180 at 1864.931 kb on - strand, within BBR_RS18180 at 1864.945 kb on - strand, within BBR_RS18180 at 1864.945 kb on - strand, within BBR_RS18180 at 1864.945 kb on - strand, within BBR_RS18180 at 1864.965 kb on - strand, within BBR_RS18180 at 1865.059 kb on - strand, within BBR_RS18180 at 1865.122 kb on - strand, within BBR_RS18180 at 1865.236 kb on - strand, within BBR_RS18180 at 1865.318 kb on - strand, within BBR_RS18180 at 1865.342 kb on - strand, within BBR_RS18180 at 1865.352 kb on - strand, within BBR_RS18180 at 1865.451 kb on - strand, within BBR_RS18180 at 1865.471 kb on + strand, within BBR_RS18180 at 1865.509 kb on - strand, within BBR_RS18180 at 1865.614 kb on - strand, within BBR_RS18180 at 1865.614 kb on - strand, within BBR_RS18180 at 1865.655 kb on - strand, within BBR_RS18180 at 1865.655 kb on - strand, within BBR_RS18180 at 1865.697 kb on - strand, within BBR_RS18180 at 1865.699 kb on + strand, within BBR_RS18180 at 1865.707 kb on - strand, within BBR_RS18180 at 1865.722 kb on + strand, within BBR_RS18180 at 1865.785 kb on - strand, within BBR_RS18180 at 1865.853 kb on - strand, within BBR_RS18180 at 1865.862 kb on - strand, within BBR_RS18180 at 1865.988 kb on - strand at 1866.010 kb on + strand at 1866.155 kb on - strand at 1866.156 kb on - strand at 1866.173 kb on - strand at 1866.173 kb on - strand at 1866.238 kb on - strand, within BBR_RS18185 at 1866.306 kb on - strand, within BBR_RS18185 at 1866.306 kb on - strand, within BBR_RS18185 at 1866.381 kb on - strand, within BBR_RS18185 at 1866.447 kb on + strand, within BBR_RS18185 at 1866.448 kb on - strand, within BBR_RS18185 at 1866.470 kb on - strand, within BBR_RS18185 at 1866.498 kb on - strand, within BBR_RS18185 at 1866.501 kb on + strand, within BBR_RS18185 at 1866.503 kb on - strand, within BBR_RS18185 at 1866.509 kb on - strand, within BBR_RS18185 at 1866.518 kb on - strand, within BBR_RS18185 at 1866.585 kb on + strand, within BBR_RS18185 at 1866.585 kb on + strand, within BBR_RS18185 at 1866.588 kb on - strand, within BBR_RS18185 at 1866.631 kb on - strand at 1866.636 kb on - strand at 1866.641 kb on - strand at 1866.656 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Lacidipine 0.4 mM remove 1,863,093 + -1.8 1,863,098 - -0.4 1,863,108 + -0.3 1,863,116 - +0.5 1,863,116 - +0.2 1,863,116 - -0.9 1,863,122 - +1.1 1,863,126 - +0.7 1,863,127 - -0.8 1,863,198 + +0.1 1,863,211 - +0.1 1,863,211 - -0.4 1,863,220 + +0.3 1,863,228 - -0.3 1,863,236 - -0.1 1,863,251 + +0.2 1,863,310 + +0.8 1,863,464 - BBR_RS18175 0.32 +0.1 1,863,489 - BBR_RS18175 0.36 +1.3 1,863,975 + +0.9 1,864,012 - -1.2 1,864,068 + -1.4 1,864,090 - -1.0 1,864,118 + +0.2 1,864,136 - -0.4 1,864,136 - -0.4 1,864,146 - +0.6 1,864,163 - -1.2 1,864,194 - +0.6 1,864,286 - BBR_RS18180 0.13 +2.5 1,864,339 - BBR_RS18180 0.15 -0.5 1,864,339 - BBR_RS18180 0.15 -2.8 1,864,339 - BBR_RS18180 0.15 +0.6 1,864,361 - BBR_RS18180 0.16 -1.8 1,864,372 + BBR_RS18180 0.17 +0.2 1,864,380 - BBR_RS18180 0.17 +0.2 1,864,499 - BBR_RS18180 0.23 +0.1 1,864,499 - BBR_RS18180 0.23 -0.1 1,864,570 - BBR_RS18180 0.26 +1.1 1,864,575 - BBR_RS18180 0.26 -1.3 1,864,593 - BBR_RS18180 0.27 -0.7 1,864,593 - BBR_RS18180 0.27 -0.7 1,864,647 - BBR_RS18180 0.30 -0.4 1,864,667 - BBR_RS18180 0.31 -2.1 1,864,683 - BBR_RS18180 0.31 -0.8 1,864,683 - BBR_RS18180 0.31 -0.0 1,864,731 - BBR_RS18180 0.34 -1.6 1,864,771 - BBR_RS18180 0.36 -1.1 1,864,795 - BBR_RS18180 0.37 -1.2 1,864,817 - BBR_RS18180 0.38 -0.9 1,864,850 - BBR_RS18180 0.39 -1.9 1,864,856 + BBR_RS18180 0.40 +0.6 1,864,931 - BBR_RS18180 0.43 -0.1 1,864,945 - BBR_RS18180 0.44 +1.6 1,864,945 - BBR_RS18180 0.44 -0.1 1,864,945 - BBR_RS18180 0.44 -1.0 1,864,965 - BBR_RS18180 0.45 -1.3 1,865,059 - BBR_RS18180 0.49 -1.8 1,865,122 - BBR_RS18180 0.52 -0.7 1,865,236 - BBR_RS18180 0.58 -2.0 1,865,318 - BBR_RS18180 0.62 -1.2 1,865,342 - BBR_RS18180 0.63 +0.5 1,865,352 - BBR_RS18180 0.63 -0.5 1,865,451 - BBR_RS18180 0.68 -0.9 1,865,471 + BBR_RS18180 0.69 -0.3 1,865,509 - BBR_RS18180 0.71 -0.6 1,865,614 - BBR_RS18180 0.76 +0.9 1,865,614 - BBR_RS18180 0.76 +0.8 1,865,655 - BBR_RS18180 0.78 +0.2 1,865,655 - BBR_RS18180 0.78 -0.7 1,865,697 - BBR_RS18180 0.80 -2.6 1,865,699 + BBR_RS18180 0.80 +0.8 1,865,707 - BBR_RS18180 0.80 -0.8 1,865,722 + BBR_RS18180 0.81 -0.3 1,865,785 - BBR_RS18180 0.84 -0.6 1,865,853 - BBR_RS18180 0.87 -0.6 1,865,862 - BBR_RS18180 0.88 +0.0 1,865,988 - +0.7 1,866,010 + +0.1 1,866,155 - -0.7 1,866,156 - +0.4 1,866,173 - -0.1 1,866,173 - -0.1 1,866,238 - BBR_RS18185 0.21 -0.1 1,866,306 - BBR_RS18185 0.33 +0.1 1,866,306 - BBR_RS18185 0.33 +0.4 1,866,381 - BBR_RS18185 0.46 -0.7 1,866,447 + BBR_RS18185 0.58 -0.5 1,866,448 - BBR_RS18185 0.58 -0.2 1,866,470 - BBR_RS18185 0.62 -1.5 1,866,498 - BBR_RS18185 0.67 +0.5 1,866,501 + BBR_RS18185 0.68 +0.1 1,866,503 - BBR_RS18185 0.68 -0.4 1,866,509 - BBR_RS18185 0.69 -0.8 1,866,518 - BBR_RS18185 0.71 +1.0 1,866,585 + BBR_RS18185 0.83 +0.7 1,866,585 + BBR_RS18185 0.83 +0.3 1,866,588 - BBR_RS18185 0.83 +0.3 1,866,631 - +0.5 1,866,636 - +0.8 1,866,641 - -1.7 1,866,656 + -0.6
Or see this region's nucleotide sequence