Experiment: Recovery broth with Lacidipine 0.4 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS17470 and BBR_RS17475 are separated by 237 nucleotides BBR_RS17475 and BBR_RS17480 are separated by 223 nucleotides BBR_RS17480 and BBR_RS17485 are separated by 116 nucleotides BBR_RS17485 and BBR_RS17490 are separated by 38 nucleotides
BBR_RS17470: BBR_RS17470 - chaperonin GroEL, at 1,699,528 to 1,701,153
_RS17470
BBR_RS17475: BBR_RS17475 - cold-shock protein, at 1,701,391 to 1,701,630
_RS17475
BBR_RS17480: BBR_RS17480 - hypothetical protein, at 1,701,854 to 1,702,510
_RS17480
BBR_RS17485: BBR_RS17485 - uracil-DNA glycosylase, at 1,702,627 to 1,703,316
_RS17485
BBR_RS17490: BBR_RS17490 - MoxR family ATPase, at 1,703,355 to 1,704,434
_RS17490
Position (kb)
1701
1702
1703 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1701.277 kb on + strand at 1701.345 kb on + strand at 1701.359 kb on + strand at 1701.359 kb on + strand at 1701.361 kb on - strand at 1701.362 kb on - strand at 1701.367 kb on - strand at 1701.388 kb on + strand at 1701.406 kb on - strand at 1701.410 kb on + strand at 1701.413 kb on - strand at 1701.418 kb on - strand, within BBR_RS17475 at 1701.463 kb on + strand, within BBR_RS17475 at 1701.464 kb on + strand, within BBR_RS17475 at 1701.464 kb on + strand, within BBR_RS17475 at 1701.464 kb on + strand, within BBR_RS17475 at 1701.464 kb on + strand, within BBR_RS17475 at 1701.466 kb on + strand, within BBR_RS17475 at 1701.466 kb on + strand, within BBR_RS17475 at 1701.472 kb on - strand, within BBR_RS17475 at 1701.472 kb on - strand, within BBR_RS17475 at 1701.474 kb on + strand, within BBR_RS17475 at 1701.474 kb on - strand, within BBR_RS17475 at 1701.474 kb on - strand, within BBR_RS17475 at 1701.493 kb on - strand, within BBR_RS17475 at 1701.552 kb on + strand, within BBR_RS17475 at 1701.579 kb on - strand, within BBR_RS17475 at 1701.617 kb on - strand at 1701.634 kb on - strand at 1701.635 kb on + strand at 1701.650 kb on + strand at 1701.658 kb on - strand at 1701.670 kb on + strand at 1701.670 kb on + strand at 1701.672 kb on + strand at 1701.673 kb on - strand at 1701.706 kb on + strand at 1701.714 kb on - strand at 1701.722 kb on - strand at 1701.732 kb on - strand at 1701.732 kb on - strand at 1701.814 kb on + strand at 1701.814 kb on - strand at 1701.865 kb on - strand at 1701.890 kb on + strand at 1701.890 kb on + strand at 1701.890 kb on + strand at 1701.898 kb on - strand at 1701.898 kb on - strand at 1701.914 kb on + strand at 1701.957 kb on + strand, within BBR_RS17480 at 1701.976 kb on - strand, within BBR_RS17480 at 1701.986 kb on + strand, within BBR_RS17480 at 1702.025 kb on + strand, within BBR_RS17480 at 1702.025 kb on + strand, within BBR_RS17480 at 1702.033 kb on - strand, within BBR_RS17480 at 1702.033 kb on - strand, within BBR_RS17480 at 1702.048 kb on - strand, within BBR_RS17480 at 1702.086 kb on + strand, within BBR_RS17480 at 1702.105 kb on + strand, within BBR_RS17480 at 1702.133 kb on - strand, within BBR_RS17480 at 1702.133 kb on - strand, within BBR_RS17480 at 1702.214 kb on + strand, within BBR_RS17480 at 1702.226 kb on - strand, within BBR_RS17480 at 1702.288 kb on - strand, within BBR_RS17480 at 1702.327 kb on - strand, within BBR_RS17480 at 1702.330 kb on - strand, within BBR_RS17480 at 1702.330 kb on - strand, within BBR_RS17480 at 1702.522 kb on + strand at 1702.530 kb on - strand at 1702.530 kb on - strand at 1702.584 kb on + strand at 1702.594 kb on + strand at 1702.594 kb on - strand at 1702.594 kb on - strand at 1702.605 kb on + strand at 1702.617 kb on + strand at 1702.620 kb on - strand at 1702.625 kb on - strand at 1702.673 kb on + strand at 1702.673 kb on - strand at 1702.673 kb on - strand at 1702.677 kb on + strand at 1702.677 kb on + strand at 1702.677 kb on + strand at 1702.742 kb on + strand, within BBR_RS17485 at 1702.742 kb on + strand, within BBR_RS17485 at 1702.776 kb on - strand, within BBR_RS17485 at 1702.785 kb on - strand, within BBR_RS17485 at 1702.785 kb on - strand, within BBR_RS17485 at 1702.785 kb on - strand, within BBR_RS17485 at 1702.819 kb on + strand, within BBR_RS17485 at 1702.819 kb on + strand, within BBR_RS17485 at 1702.827 kb on + strand, within BBR_RS17485 at 1702.827 kb on + strand, within BBR_RS17485 at 1702.828 kb on + strand, within BBR_RS17485 at 1702.891 kb on + strand, within BBR_RS17485 at 1702.891 kb on + strand, within BBR_RS17485 at 1702.899 kb on - strand, within BBR_RS17485 at 1702.899 kb on - strand, within BBR_RS17485 at 1702.900 kb on + strand, within BBR_RS17485 at 1702.913 kb on - strand, within BBR_RS17485 at 1702.913 kb on - strand, within BBR_RS17485 at 1702.916 kb on + strand, within BBR_RS17485 at 1703.015 kb on - strand, within BBR_RS17485 at 1703.016 kb on + strand, within BBR_RS17485 at 1703.031 kb on + strand, within BBR_RS17485 at 1703.039 kb on - strand, within BBR_RS17485 at 1703.039 kb on - strand, within BBR_RS17485 at 1703.039 kb on - strand, within BBR_RS17485 at 1703.046 kb on + strand, within BBR_RS17485 at 1703.046 kb on + strand, within BBR_RS17485 at 1703.053 kb on + strand, within BBR_RS17485 at 1703.065 kb on - strand, within BBR_RS17485 at 1703.087 kb on + strand, within BBR_RS17485 at 1703.106 kb on - strand, within BBR_RS17485 at 1703.137 kb on + strand, within BBR_RS17485 at 1703.145 kb on - strand, within BBR_RS17485 at 1703.162 kb on + strand, within BBR_RS17485 at 1703.162 kb on + strand, within BBR_RS17485 at 1703.162 kb on + strand, within BBR_RS17485 at 1703.162 kb on + strand, within BBR_RS17485 at 1703.162 kb on + strand, within BBR_RS17485 at 1703.162 kb on + strand, within BBR_RS17485 at 1703.170 kb on - strand, within BBR_RS17485 at 1703.170 kb on - strand, within BBR_RS17485 at 1703.170 kb on - strand, within BBR_RS17485 at 1703.243 kb on + strand, within BBR_RS17485 at 1703.262 kb on + strand at 1703.266 kb on + strand at 1703.266 kb on + strand at 1703.266 kb on + strand at 1703.266 kb on + strand at 1703.274 kb on - strand at 1703.274 kb on - strand at 1703.283 kb on - strand at 1703.293 kb on - strand at 1703.302 kb on + strand at 1703.319 kb on - strand at 1703.319 kb on - strand at 1703.339 kb on + strand at 1703.421 kb on + strand at 1703.429 kb on - strand at 1703.476 kb on + strand, within BBR_RS17490 at 1703.482 kb on - strand, within BBR_RS17490 at 1703.496 kb on - strand, within BBR_RS17490
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Lacidipine 0.4 mM remove 1,701,277 + +0.2 1,701,345 + -0.2 1,701,359 + -0.1 1,701,359 + -0.5 1,701,361 - -1.5 1,701,362 - -0.3 1,701,367 - +1.6 1,701,388 + +0.6 1,701,406 - +0.6 1,701,410 + -1.0 1,701,413 - +0.2 1,701,418 - BBR_RS17475 0.11 +0.5 1,701,463 + BBR_RS17475 0.30 +0.5 1,701,464 + BBR_RS17475 0.30 +0.3 1,701,464 + BBR_RS17475 0.30 -0.9 1,701,464 + BBR_RS17475 0.30 +0.4 1,701,464 + BBR_RS17475 0.30 +0.4 1,701,466 + BBR_RS17475 0.31 +0.3 1,701,466 + BBR_RS17475 0.31 -1.3 1,701,472 - BBR_RS17475 0.34 +0.8 1,701,472 - BBR_RS17475 0.34 +0.9 1,701,474 + BBR_RS17475 0.35 +0.4 1,701,474 - BBR_RS17475 0.35 -0.2 1,701,474 - BBR_RS17475 0.35 -0.6 1,701,493 - BBR_RS17475 0.42 +0.1 1,701,552 + BBR_RS17475 0.67 +0.6 1,701,579 - BBR_RS17475 0.78 -0.0 1,701,617 - +0.6 1,701,634 - +0.1 1,701,635 + +0.8 1,701,650 + -0.5 1,701,658 - +0.4 1,701,670 + +0.0 1,701,670 + +0.2 1,701,672 + +1.0 1,701,673 - -0.3 1,701,706 + +0.3 1,701,714 - +0.3 1,701,722 - -0.5 1,701,732 - +0.7 1,701,732 - +0.2 1,701,814 + -0.2 1,701,814 - -0.6 1,701,865 - +0.1 1,701,890 + -1.0 1,701,890 + +0.6 1,701,890 + -0.8 1,701,898 - +0.6 1,701,898 - +2.6 1,701,914 + -0.3 1,701,957 + BBR_RS17480 0.16 -0.6 1,701,976 - BBR_RS17480 0.19 -0.3 1,701,986 + BBR_RS17480 0.20 -0.2 1,702,025 + BBR_RS17480 0.26 -0.4 1,702,025 + BBR_RS17480 0.26 +0.1 1,702,033 - BBR_RS17480 0.27 -1.1 1,702,033 - BBR_RS17480 0.27 -1.7 1,702,048 - BBR_RS17480 0.30 +0.5 1,702,086 + BBR_RS17480 0.35 -1.3 1,702,105 + BBR_RS17480 0.38 -0.3 1,702,133 - BBR_RS17480 0.42 -1.3 1,702,133 - BBR_RS17480 0.42 +0.1 1,702,214 + BBR_RS17480 0.55 +0.1 1,702,226 - BBR_RS17480 0.57 -0.8 1,702,288 - BBR_RS17480 0.66 -1.8 1,702,327 - BBR_RS17480 0.72 +1.4 1,702,330 - BBR_RS17480 0.72 -0.5 1,702,330 - BBR_RS17480 0.72 -0.7 1,702,522 + -0.3 1,702,530 - +0.0 1,702,530 - +0.1 1,702,584 + -0.5 1,702,594 + -0.0 1,702,594 - -1.1 1,702,594 - -1.2 1,702,605 + -0.6 1,702,617 + -0.9 1,702,620 - +0.4 1,702,625 - -0.0 1,702,673 + -0.5 1,702,673 - +0.0 1,702,673 - -0.3 1,702,677 + +0.2 1,702,677 + +0.7 1,702,677 + +0.6 1,702,742 + BBR_RS17485 0.17 +0.4 1,702,742 + BBR_RS17485 0.17 +0.6 1,702,776 - BBR_RS17485 0.22 -0.1 1,702,785 - BBR_RS17485 0.23 +1.0 1,702,785 - BBR_RS17485 0.23 -2.2 1,702,785 - BBR_RS17485 0.23 +0.2 1,702,819 + BBR_RS17485 0.28 +0.8 1,702,819 + BBR_RS17485 0.28 -0.7 1,702,827 + BBR_RS17485 0.29 +0.3 1,702,827 + BBR_RS17485 0.29 +0.7 1,702,828 + BBR_RS17485 0.29 +0.4 1,702,891 + BBR_RS17485 0.38 +0.1 1,702,891 + BBR_RS17485 0.38 -1.2 1,702,899 - BBR_RS17485 0.39 +1.3 1,702,899 - BBR_RS17485 0.39 -0.7 1,702,900 + BBR_RS17485 0.40 -0.1 1,702,913 - BBR_RS17485 0.41 -0.8 1,702,913 - BBR_RS17485 0.41 -0.6 1,702,916 + BBR_RS17485 0.42 +0.0 1,703,015 - BBR_RS17485 0.56 -0.6 1,703,016 + BBR_RS17485 0.56 +1.6 1,703,031 + BBR_RS17485 0.59 -0.8 1,703,039 - BBR_RS17485 0.60 -0.3 1,703,039 - BBR_RS17485 0.60 -0.3 1,703,039 - BBR_RS17485 0.60 -1.1 1,703,046 + BBR_RS17485 0.61 -0.5 1,703,046 + BBR_RS17485 0.61 -0.7 1,703,053 + BBR_RS17485 0.62 -0.3 1,703,065 - BBR_RS17485 0.63 +0.2 1,703,087 + BBR_RS17485 0.67 -0.5 1,703,106 - BBR_RS17485 0.69 +0.0 1,703,137 + BBR_RS17485 0.74 +0.4 1,703,145 - BBR_RS17485 0.75 -0.1 1,703,162 + BBR_RS17485 0.78 +0.2 1,703,162 + BBR_RS17485 0.78 +0.7 1,703,162 + BBR_RS17485 0.78 +1.0 1,703,162 + BBR_RS17485 0.78 +0.9 1,703,162 + BBR_RS17485 0.78 -0.2 1,703,162 + BBR_RS17485 0.78 +0.5 1,703,170 - BBR_RS17485 0.79 +1.0 1,703,170 - BBR_RS17485 0.79 +0.3 1,703,170 - BBR_RS17485 0.79 +0.1 1,703,243 + BBR_RS17485 0.89 -0.7 1,703,262 + -0.4 1,703,266 + +0.7 1,703,266 + +0.3 1,703,266 + +0.2 1,703,266 + +0.0 1,703,274 - +0.6 1,703,274 - +0.5 1,703,283 - -1.1 1,703,293 - +0.5 1,703,302 + -0.3 1,703,319 - +0.2 1,703,319 - -0.4 1,703,339 + +0.3 1,703,421 + -0.2 1,703,429 - -0.9 1,703,476 + BBR_RS17490 0.11 +1.1 1,703,482 - BBR_RS17490 0.12 -0.9 1,703,496 - BBR_RS17490 0.13 +0.1
Or see this region's nucleotide sequence