Experiment: Recovery broth with Lacidipine 0.4 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS16975 and BBR_RS16980 are separated by 53 nucleotides BBR_RS16980 and BBR_RS16985 are separated by 150 nucleotides BBR_RS16985 and BBR_RS16990 are separated by 150 nucleotides
BBR_RS16975: BBR_RS16975 - transcriptional repressor NrdR, at 1,581,786 to 1,582,226
_RS16975
BBR_RS16980: BBR_RS16980 - LysM peptidoglycan-binding domain-containing protein, at 1,582,280 to 1,582,663
_RS16980
BBR_RS16985: BBR_RS16985 - transcriptional repressor LexA, at 1,582,814 to 1,583,527
_RS16985
BBR_RS16990: BBR_RS16990 - cation transporter, at 1,583,678 to 1,584,622
_RS16990
Position (kb)
1582
1583
1584 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1581.875 kb on + strand, within BBR_RS16975 at 1581.917 kb on + strand, within BBR_RS16975 at 1581.946 kb on - strand, within BBR_RS16975 at 1581.946 kb on - strand, within BBR_RS16975 at 1581.946 kb on - strand, within BBR_RS16975 at 1581.989 kb on - strand, within BBR_RS16975 at 1582.003 kb on + strand, within BBR_RS16975 at 1582.018 kb on + strand, within BBR_RS16975 at 1582.026 kb on - strand, within BBR_RS16975 at 1582.058 kb on + strand, within BBR_RS16975 at 1582.066 kb on - strand, within BBR_RS16975 at 1582.238 kb on - strand at 1582.247 kb on - strand at 1582.294 kb on + strand at 1582.302 kb on - strand at 1582.304 kb on - strand at 1582.320 kb on + strand, within BBR_RS16980 at 1582.357 kb on - strand, within BBR_RS16980 at 1582.372 kb on - strand, within BBR_RS16980 at 1582.422 kb on - strand, within BBR_RS16980 at 1582.432 kb on + strand, within BBR_RS16980 at 1582.440 kb on - strand, within BBR_RS16980 at 1582.516 kb on - strand, within BBR_RS16980 at 1582.516 kb on - strand, within BBR_RS16980 at 1582.533 kb on + strand, within BBR_RS16980 at 1582.541 kb on - strand, within BBR_RS16980 at 1582.541 kb on - strand, within BBR_RS16980 at 1582.566 kb on + strand, within BBR_RS16980 at 1582.566 kb on + strand, within BBR_RS16980 at 1582.580 kb on - strand, within BBR_RS16980 at 1582.580 kb on - strand, within BBR_RS16980 at 1582.635 kb on - strand at 1582.641 kb on + strand at 1582.772 kb on + strand at 1582.791 kb on - strand at 1582.804 kb on - strand at 1582.816 kb on + strand at 1582.819 kb on - strand at 1583.100 kb on + strand, within BBR_RS16985 at 1583.108 kb on - strand, within BBR_RS16985 at 1583.192 kb on + strand, within BBR_RS16985 at 1583.220 kb on + strand, within BBR_RS16985 at 1583.282 kb on - strand, within BBR_RS16985 at 1583.328 kb on + strand, within BBR_RS16985 at 1583.383 kb on + strand, within BBR_RS16985 at 1583.395 kb on + strand, within BBR_RS16985 at 1583.410 kb on - strand, within BBR_RS16985 at 1583.410 kb on - strand, within BBR_RS16985 at 1583.431 kb on + strand, within BBR_RS16985 at 1583.439 kb on - strand, within BBR_RS16985 at 1583.461 kb on + strand at 1583.505 kb on + strand at 1583.505 kb on + strand at 1583.518 kb on - strand at 1583.654 kb on - strand at 1583.655 kb on + strand at 1583.689 kb on - strand at 1583.696 kb on - strand at 1583.743 kb on - strand at 1583.746 kb on + strand at 1583.769 kb on - strand at 1583.802 kb on - strand, within BBR_RS16990 at 1583.817 kb on + strand, within BBR_RS16990 at 1583.817 kb on + strand, within BBR_RS16990 at 1583.851 kb on - strand, within BBR_RS16990 at 1583.851 kb on - strand, within BBR_RS16990 at 1583.859 kb on + strand, within BBR_RS16990 at 1583.891 kb on + strand, within BBR_RS16990 at 1583.894 kb on - strand, within BBR_RS16990 at 1583.923 kb on + strand, within BBR_RS16990 at 1583.923 kb on + strand, within BBR_RS16990 at 1583.978 kb on - strand, within BBR_RS16990 at 1583.983 kb on - strand, within BBR_RS16990 at 1584.002 kb on + strand, within BBR_RS16990 at 1584.004 kb on + strand, within BBR_RS16990 at 1584.139 kb on - strand, within BBR_RS16990 at 1584.154 kb on + strand, within BBR_RS16990 at 1584.184 kb on + strand, within BBR_RS16990 at 1584.198 kb on - strand, within BBR_RS16990 at 1584.200 kb on + strand, within BBR_RS16990 at 1584.200 kb on + strand, within BBR_RS16990 at 1584.251 kb on + strand, within BBR_RS16990 at 1584.259 kb on - strand, within BBR_RS16990 at 1584.307 kb on + strand, within BBR_RS16990 at 1584.316 kb on + strand, within BBR_RS16990 at 1584.321 kb on + strand, within BBR_RS16990 at 1584.321 kb on + strand, within BBR_RS16990 at 1584.324 kb on - strand, within BBR_RS16990 at 1584.347 kb on - strand, within BBR_RS16990 at 1584.347 kb on - strand, within BBR_RS16990 at 1584.355 kb on + strand, within BBR_RS16990 at 1584.356 kb on - strand, within BBR_RS16990 at 1584.371 kb on - strand, within BBR_RS16990 at 1584.374 kb on + strand, within BBR_RS16990 at 1584.377 kb on + strand, within BBR_RS16990 at 1584.436 kb on - strand, within BBR_RS16990 at 1584.462 kb on + strand, within BBR_RS16990 at 1584.475 kb on + strand, within BBR_RS16990 at 1584.491 kb on - strand, within BBR_RS16990
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Lacidipine 0.4 mM remove 1,581,875 + BBR_RS16975 0.20 +1.2 1,581,917 + BBR_RS16975 0.30 +1.5 1,581,946 - BBR_RS16975 0.36 +1.8 1,581,946 - BBR_RS16975 0.36 +0.8 1,581,946 - BBR_RS16975 0.36 +0.3 1,581,989 - BBR_RS16975 0.46 +1.3 1,582,003 + BBR_RS16975 0.49 -0.3 1,582,018 + BBR_RS16975 0.53 +0.7 1,582,026 - BBR_RS16975 0.54 +0.7 1,582,058 + BBR_RS16975 0.62 -0.3 1,582,066 - BBR_RS16975 0.63 +1.7 1,582,238 - -0.1 1,582,247 - +1.8 1,582,294 + +0.0 1,582,302 - +1.0 1,582,304 - -0.2 1,582,320 + BBR_RS16980 0.10 -1.8 1,582,357 - BBR_RS16980 0.20 +0.1 1,582,372 - BBR_RS16980 0.24 -0.2 1,582,422 - BBR_RS16980 0.37 -1.0 1,582,432 + BBR_RS16980 0.40 -0.1 1,582,440 - BBR_RS16980 0.42 +0.2 1,582,516 - BBR_RS16980 0.61 +1.0 1,582,516 - BBR_RS16980 0.61 -0.2 1,582,533 + BBR_RS16980 0.66 +0.4 1,582,541 - BBR_RS16980 0.68 -0.2 1,582,541 - BBR_RS16980 0.68 -0.3 1,582,566 + BBR_RS16980 0.74 +0.5 1,582,566 + BBR_RS16980 0.74 -1.0 1,582,580 - BBR_RS16980 0.78 +0.3 1,582,580 - BBR_RS16980 0.78 -0.3 1,582,635 - -0.0 1,582,641 + -0.2 1,582,772 + +0.7 1,582,791 - +1.2 1,582,804 - -0.6 1,582,816 + -0.0 1,582,819 - -0.6 1,583,100 + BBR_RS16985 0.40 -1.0 1,583,108 - BBR_RS16985 0.41 -0.1 1,583,192 + BBR_RS16985 0.53 +0.5 1,583,220 + BBR_RS16985 0.57 -0.1 1,583,282 - BBR_RS16985 0.66 -1.6 1,583,328 + BBR_RS16985 0.72 +0.3 1,583,383 + BBR_RS16985 0.80 -2.6 1,583,395 + BBR_RS16985 0.81 -0.4 1,583,410 - BBR_RS16985 0.83 +0.2 1,583,410 - BBR_RS16985 0.83 +0.2 1,583,431 + BBR_RS16985 0.86 -1.8 1,583,439 - BBR_RS16985 0.88 -0.5 1,583,461 + -0.8 1,583,505 + +0.2 1,583,505 + +0.3 1,583,518 - -1.1 1,583,654 - -0.3 1,583,655 + -0.2 1,583,689 - +0.3 1,583,696 - -0.0 1,583,743 - -0.6 1,583,746 + -1.5 1,583,769 - +3.4 1,583,802 - BBR_RS16990 0.13 +0.7 1,583,817 + BBR_RS16990 0.15 +0.4 1,583,817 + BBR_RS16990 0.15 -0.4 1,583,851 - BBR_RS16990 0.18 -0.4 1,583,851 - BBR_RS16990 0.18 -0.7 1,583,859 + BBR_RS16990 0.19 -0.1 1,583,891 + BBR_RS16990 0.23 +0.2 1,583,894 - BBR_RS16990 0.23 -0.9 1,583,923 + BBR_RS16990 0.26 +0.8 1,583,923 + BBR_RS16990 0.26 +0.5 1,583,978 - BBR_RS16990 0.32 +0.0 1,583,983 - BBR_RS16990 0.32 -1.1 1,584,002 + BBR_RS16990 0.34 -0.6 1,584,004 + BBR_RS16990 0.34 -1.2 1,584,139 - BBR_RS16990 0.49 -0.0 1,584,154 + BBR_RS16990 0.50 +0.2 1,584,184 + BBR_RS16990 0.54 +0.4 1,584,198 - BBR_RS16990 0.55 +0.7 1,584,200 + BBR_RS16990 0.55 -0.3 1,584,200 + BBR_RS16990 0.55 -0.1 1,584,251 + BBR_RS16990 0.61 +0.8 1,584,259 - BBR_RS16990 0.61 -1.5 1,584,307 + BBR_RS16990 0.67 +0.2 1,584,316 + BBR_RS16990 0.68 +0.9 1,584,321 + BBR_RS16990 0.68 -0.6 1,584,321 + BBR_RS16990 0.68 -0.5 1,584,324 - BBR_RS16990 0.68 -0.1 1,584,347 - BBR_RS16990 0.71 +0.7 1,584,347 - BBR_RS16990 0.71 -0.4 1,584,355 + BBR_RS16990 0.72 -0.5 1,584,356 - BBR_RS16990 0.72 -0.5 1,584,371 - BBR_RS16990 0.73 +1.8 1,584,374 + BBR_RS16990 0.74 +0.2 1,584,377 + BBR_RS16990 0.74 -0.5 1,584,436 - BBR_RS16990 0.80 +0.7 1,584,462 + BBR_RS16990 0.83 +0.1 1,584,475 + BBR_RS16990 0.84 -0.2 1,584,491 - BBR_RS16990 0.86 +0.2
Or see this region's nucleotide sequence