Experiment: Recovery broth with Lacidipine 0.4 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS16810 and BBR_RS16815 are separated by 261 nucleotides BBR_RS16815 and BBR_RS16820 overlap by 4 nucleotides BBR_RS16820 and BBR_RS16825 are separated by 150 nucleotides
BBR_RS16810: BBR_RS16810 - aldose 1-epimerase, at 1,540,691 to 1,541,635
_RS16810
BBR_RS16815: BBR_RS16815 - hypothetical protein, at 1,541,897 to 1,542,205
_RS16815
BBR_RS16820: BBR_RS16820 - RNA polymerase sigma factor RpoE, at 1,542,202 to 1,542,963
_RS16820
BBR_RS16825: BBR_RS16825 - UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2, 6-diaminopimelate ligase, at 1,543,114 to 1,544,661
_RS16825
Position (kb)
1542
1543 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1541.215 kb on + strand, within BBR_RS16810 at 1541.259 kb on + strand, within BBR_RS16810 at 1541.261 kb on + strand, within BBR_RS16810 at 1541.267 kb on - strand, within BBR_RS16810 at 1541.268 kb on + strand, within BBR_RS16810 at 1541.268 kb on + strand, within BBR_RS16810 at 1541.269 kb on - strand, within BBR_RS16810 at 1541.271 kb on - strand, within BBR_RS16810 at 1541.272 kb on - strand, within BBR_RS16810 at 1541.291 kb on - strand, within BBR_RS16810 at 1541.320 kb on - strand, within BBR_RS16810 at 1541.331 kb on + strand, within BBR_RS16810 at 1541.331 kb on + strand, within BBR_RS16810 at 1541.331 kb on + strand, within BBR_RS16810 at 1541.331 kb on + strand, within BBR_RS16810 at 1541.331 kb on + strand, within BBR_RS16810 at 1541.331 kb on + strand, within BBR_RS16810 at 1541.331 kb on + strand, within BBR_RS16810 at 1541.331 kb on + strand, within BBR_RS16810 at 1541.339 kb on - strand, within BBR_RS16810 at 1541.356 kb on + strand, within BBR_RS16810 at 1541.382 kb on + strand, within BBR_RS16810 at 1541.397 kb on + strand, within BBR_RS16810 at 1541.397 kb on + strand, within BBR_RS16810 at 1541.397 kb on + strand, within BBR_RS16810 at 1541.402 kb on + strand, within BBR_RS16810 at 1541.405 kb on + strand, within BBR_RS16810 at 1541.405 kb on - strand, within BBR_RS16810 at 1541.413 kb on - strand, within BBR_RS16810 at 1541.435 kb on + strand, within BBR_RS16810 at 1541.470 kb on + strand, within BBR_RS16810 at 1541.516 kb on - strand, within BBR_RS16810 at 1541.535 kb on - strand, within BBR_RS16810 at 1541.545 kb on - strand at 1541.567 kb on + strand at 1541.570 kb on - strand at 1541.594 kb on + strand at 1541.594 kb on + strand at 1541.601 kb on + strand at 1541.602 kb on - strand at 1541.614 kb on + strand at 1541.619 kb on + strand at 1541.619 kb on + strand at 1541.629 kb on + strand at 1541.629 kb on + strand at 1541.629 kb on + strand at 1541.629 kb on + strand at 1541.632 kb on - strand at 1541.637 kb on - strand at 1541.656 kb on + strand at 1541.656 kb on + strand at 1541.664 kb on - strand at 1541.664 kb on - strand at 1541.664 kb on - strand at 1541.666 kb on - strand at 1541.725 kb on + strand at 1541.916 kb on - strand at 1542.240 kb on - strand at 1542.292 kb on + strand, within BBR_RS16820 at 1542.448 kb on - strand, within BBR_RS16820 at 1542.585 kb on - strand, within BBR_RS16820 at 1542.635 kb on - strand, within BBR_RS16820 at 1542.637 kb on + strand, within BBR_RS16820 at 1542.718 kb on + strand, within BBR_RS16820 at 1542.801 kb on + strand, within BBR_RS16820 at 1542.975 kb on - strand at 1543.043 kb on + strand at 1543.074 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Lacidipine 0.4 mM remove 1,541,215 + BBR_RS16810 0.55 -0.4 1,541,259 + BBR_RS16810 0.60 +1.6 1,541,261 + BBR_RS16810 0.60 -0.5 1,541,267 - BBR_RS16810 0.61 +0.6 1,541,268 + BBR_RS16810 0.61 +0.4 1,541,268 + BBR_RS16810 0.61 -0.3 1,541,269 - BBR_RS16810 0.61 -0.3 1,541,271 - BBR_RS16810 0.61 +0.6 1,541,272 - BBR_RS16810 0.61 -0.5 1,541,291 - BBR_RS16810 0.63 +0.3 1,541,320 - BBR_RS16810 0.67 -0.4 1,541,331 + BBR_RS16810 0.68 +0.4 1,541,331 + BBR_RS16810 0.68 +0.0 1,541,331 + BBR_RS16810 0.68 -0.7 1,541,331 + BBR_RS16810 0.68 +0.2 1,541,331 + BBR_RS16810 0.68 -0.1 1,541,331 + BBR_RS16810 0.68 +0.5 1,541,331 + BBR_RS16810 0.68 -0.4 1,541,331 + BBR_RS16810 0.68 -0.3 1,541,339 - BBR_RS16810 0.69 +0.2 1,541,356 + BBR_RS16810 0.70 -0.8 1,541,382 + BBR_RS16810 0.73 -0.4 1,541,397 + BBR_RS16810 0.75 +1.0 1,541,397 + BBR_RS16810 0.75 -0.1 1,541,397 + BBR_RS16810 0.75 +0.4 1,541,402 + BBR_RS16810 0.75 -0.6 1,541,405 + BBR_RS16810 0.76 +0.0 1,541,405 - BBR_RS16810 0.76 -0.6 1,541,413 - BBR_RS16810 0.76 +0.7 1,541,435 + BBR_RS16810 0.79 +1.1 1,541,470 + BBR_RS16810 0.82 -0.9 1,541,516 - BBR_RS16810 0.87 -0.1 1,541,535 - BBR_RS16810 0.89 -1.3 1,541,545 - -1.1 1,541,567 + -1.7 1,541,570 - -0.6 1,541,594 + +1.0 1,541,594 + -0.3 1,541,601 + +0.8 1,541,602 - -0.1 1,541,614 + -1.5 1,541,619 + -0.2 1,541,619 + +0.2 1,541,629 + +0.7 1,541,629 + -0.0 1,541,629 + +0.4 1,541,629 + -0.0 1,541,632 - +0.2 1,541,637 - +1.4 1,541,656 + -0.4 1,541,656 + +0.3 1,541,664 - +2.0 1,541,664 - -0.9 1,541,664 - -0.3 1,541,666 - +0.7 1,541,725 + +0.6 1,541,916 - +0.3 1,542,240 - +0.0 1,542,292 + BBR_RS16820 0.12 -1.6 1,542,448 - BBR_RS16820 0.32 -0.1 1,542,585 - BBR_RS16820 0.50 +2.8 1,542,635 - BBR_RS16820 0.57 -0.1 1,542,637 + BBR_RS16820 0.57 -1.5 1,542,718 + BBR_RS16820 0.68 +0.7 1,542,801 + BBR_RS16820 0.79 -2.2 1,542,975 - +0.5 1,543,043 + -0.8 1,543,074 + -1.7
Or see this region's nucleotide sequence