Strain Fitness in Bifidobacterium breve UCC2003 around BBR_RS15880

Experiment: Recovery broth with Lacidipine 0.4 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBBR_RS15875 and BBR_RS15880 are separated by 162 nucleotidesBBR_RS15880 and BBR_RS20950 are separated by 592 nucleotidesBBR_RS20950 and BBR_RS20955 overlap by 59 nucleotides BBR_RS15875: BBR_RS15875 - DNA-binding protein, at 1,324,662 to 1,325,468 _RS15875 BBR_RS15880: BBR_RS15880 - hypothetical protein, at 1,325,631 to 1,325,885 _RS15880 BBR_RS20950: BBR_RS20950 - hypothetical protein, at 1,326,478 to 1,326,684 _RS20950 BBR_RS20955: BBR_RS20955 - hypothetical protein, at 1,326,626 to 1,326,813 _RS20955 Position (kb) 1325 1326Strain fitness (log2 ratio) -2 -1 0 1at 1324.762 kb on + strand, within BBR_RS15875at 1324.764 kb on + strand, within BBR_RS15875at 1324.767 kb on + strand, within BBR_RS15875at 1324.902 kb on + strand, within BBR_RS15875at 1324.926 kb on + strand, within BBR_RS15875at 1324.927 kb on - strand, within BBR_RS15875at 1324.933 kb on - strand, within BBR_RS15875at 1324.959 kb on - strand, within BBR_RS15875at 1324.998 kb on + strand, within BBR_RS15875at 1324.998 kb on + strand, within BBR_RS15875at 1325.006 kb on - strand, within BBR_RS15875at 1325.006 kb on - strand, within BBR_RS15875at 1325.006 kb on - strand, within BBR_RS15875at 1325.006 kb on - strand, within BBR_RS15875at 1325.006 kb on - strand, within BBR_RS15875at 1325.006 kb on - strand, within BBR_RS15875at 1325.011 kb on - strand, within BBR_RS15875at 1325.011 kb on - strand, within BBR_RS15875at 1325.026 kb on + strand, within BBR_RS15875at 1325.035 kb on + strand, within BBR_RS15875at 1325.081 kb on - strand, within BBR_RS15875at 1325.116 kb on - strand, within BBR_RS15875at 1325.132 kb on - strand, within BBR_RS15875at 1325.202 kb on - strand, within BBR_RS15875at 1325.232 kb on + strand, within BBR_RS15875at 1325.303 kb on + strand, within BBR_RS15875at 1325.389 kb on + strandat 1325.395 kb on + strandat 1325.418 kb on - strandat 1325.579 kb on + strandat 1325.634 kb on + strandat 1325.720 kb on - strand, within BBR_RS15880at 1325.737 kb on + strand, within BBR_RS15880at 1325.791 kb on + strand, within BBR_RS15880at 1325.804 kb on - strand, within BBR_RS15880at 1325.823 kb on + strand, within BBR_RS15880at 1325.998 kb on + strandat 1326.000 kb on - strandat 1326.000 kb on - strandat 1326.021 kb on - strandat 1326.034 kb on + strandat 1326.132 kb on + strandat 1326.172 kb on - strandat 1326.172 kb on - strandat 1326.204 kb on - strandat 1326.412 kb on + strandat 1326.435 kb on + strandat 1326.459 kb on - strandat 1326.739 kb on + strand, within BBR_RS20955at 1326.739 kb on + strand, within BBR_RS20955at 1326.807 kb on + strandat 1326.815 kb on - strandat 1326.864 kb on + strandat 1326.866 kb on + strandat 1326.866 kb on + strandat 1326.874 kb on - strandat 1326.874 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Recovery broth with Lacidipine 0.4 mM
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1,324,762 + BBR_RS15875 0.12 -2.1
1,324,764 + BBR_RS15875 0.13 +0.4
1,324,767 + BBR_RS15875 0.13 +0.2
1,324,902 + BBR_RS15875 0.30 -1.3
1,324,926 + BBR_RS15875 0.33 +0.2
1,324,927 - BBR_RS15875 0.33 +0.2
1,324,933 - BBR_RS15875 0.34 -0.2
1,324,959 - BBR_RS15875 0.37 -0.8
1,324,998 + BBR_RS15875 0.42 -0.2
1,324,998 + BBR_RS15875 0.42 +0.4
1,325,006 - BBR_RS15875 0.43 +0.1
1,325,006 - BBR_RS15875 0.43 -1.1
1,325,006 - BBR_RS15875 0.43 -0.1
1,325,006 - BBR_RS15875 0.43 +0.1
1,325,006 - BBR_RS15875 0.43 -1.5
1,325,006 - BBR_RS15875 0.43 +0.3
1,325,011 - BBR_RS15875 0.43 +1.1
1,325,011 - BBR_RS15875 0.43 +0.5
1,325,026 + BBR_RS15875 0.45 +0.2
1,325,035 + BBR_RS15875 0.46 -0.0
1,325,081 - BBR_RS15875 0.52 +0.1
1,325,116 - BBR_RS15875 0.56 +1.1
1,325,132 - BBR_RS15875 0.58 -1.4
1,325,202 - BBR_RS15875 0.67 +0.8
1,325,232 + BBR_RS15875 0.71 +0.5
1,325,303 + BBR_RS15875 0.79 +0.2
1,325,389 + +0.2
1,325,395 + +0.3
1,325,418 - -0.1
1,325,579 + +0.2
1,325,634 + -0.3
1,325,720 - BBR_RS15880 0.35 -0.6
1,325,737 + BBR_RS15880 0.42 -1.1
1,325,791 + BBR_RS15880 0.63 -0.2
1,325,804 - BBR_RS15880 0.68 -0.3
1,325,823 + BBR_RS15880 0.75 +0.1
1,325,998 + +0.3
1,326,000 - -0.5
1,326,000 - +0.4
1,326,021 - +1.0
1,326,034 + +1.4
1,326,132 + -1.4
1,326,172 - -0.2
1,326,172 - -0.5
1,326,204 - +1.1
1,326,412 + +0.5
1,326,435 + +0.0
1,326,459 - -0.4
1,326,739 + BBR_RS20955 0.60 -0.0
1,326,739 + BBR_RS20955 0.60 -1.7
1,326,807 + +0.2
1,326,815 - +1.0
1,326,864 + +0.2
1,326,866 + +0.2
1,326,866 + -0.9
1,326,874 - +1.4
1,326,874 - -1.3

Or see this region's nucleotide sequence