Experiment: Recovery broth with Lacidipine 0.4 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS15875 and BBR_RS15880 are separated by 162 nucleotides BBR_RS15880 and BBR_RS20950 are separated by 592 nucleotides BBR_RS20950 and BBR_RS20955 overlap by 59 nucleotides
BBR_RS15875: BBR_RS15875 - DNA-binding protein, at 1,324,662 to 1,325,468
_RS15875
BBR_RS15880: BBR_RS15880 - hypothetical protein, at 1,325,631 to 1,325,885
_RS15880
BBR_RS20950: BBR_RS20950 - hypothetical protein, at 1,326,478 to 1,326,684
_RS20950
BBR_RS20955: BBR_RS20955 - hypothetical protein, at 1,326,626 to 1,326,813
_RS20955
Position (kb)
1325
1326 Strain fitness (log2 ratio)
-2
-1
0
1 at 1324.762 kb on + strand, within BBR_RS15875 at 1324.764 kb on + strand, within BBR_RS15875 at 1324.767 kb on + strand, within BBR_RS15875 at 1324.902 kb on + strand, within BBR_RS15875 at 1324.926 kb on + strand, within BBR_RS15875 at 1324.927 kb on - strand, within BBR_RS15875 at 1324.933 kb on - strand, within BBR_RS15875 at 1324.959 kb on - strand, within BBR_RS15875 at 1324.998 kb on + strand, within BBR_RS15875 at 1324.998 kb on + strand, within BBR_RS15875 at 1325.006 kb on - strand, within BBR_RS15875 at 1325.006 kb on - strand, within BBR_RS15875 at 1325.006 kb on - strand, within BBR_RS15875 at 1325.006 kb on - strand, within BBR_RS15875 at 1325.006 kb on - strand, within BBR_RS15875 at 1325.006 kb on - strand, within BBR_RS15875 at 1325.011 kb on - strand, within BBR_RS15875 at 1325.011 kb on - strand, within BBR_RS15875 at 1325.026 kb on + strand, within BBR_RS15875 at 1325.035 kb on + strand, within BBR_RS15875 at 1325.081 kb on - strand, within BBR_RS15875 at 1325.116 kb on - strand, within BBR_RS15875 at 1325.132 kb on - strand, within BBR_RS15875 at 1325.202 kb on - strand, within BBR_RS15875 at 1325.232 kb on + strand, within BBR_RS15875 at 1325.303 kb on + strand, within BBR_RS15875 at 1325.389 kb on + strand at 1325.395 kb on + strand at 1325.418 kb on - strand at 1325.579 kb on + strand at 1325.634 kb on + strand at 1325.720 kb on - strand, within BBR_RS15880 at 1325.737 kb on + strand, within BBR_RS15880 at 1325.791 kb on + strand, within BBR_RS15880 at 1325.804 kb on - strand, within BBR_RS15880 at 1325.823 kb on + strand, within BBR_RS15880 at 1325.998 kb on + strand at 1326.000 kb on - strand at 1326.000 kb on - strand at 1326.021 kb on - strand at 1326.034 kb on + strand at 1326.132 kb on + strand at 1326.172 kb on - strand at 1326.172 kb on - strand at 1326.204 kb on - strand at 1326.412 kb on + strand at 1326.435 kb on + strand at 1326.459 kb on - strand at 1326.739 kb on + strand, within BBR_RS20955 at 1326.739 kb on + strand, within BBR_RS20955 at 1326.807 kb on + strand at 1326.815 kb on - strand at 1326.864 kb on + strand at 1326.866 kb on + strand at 1326.866 kb on + strand at 1326.874 kb on - strand at 1326.874 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Lacidipine 0.4 mM remove 1,324,762 + BBR_RS15875 0.12 -2.1 1,324,764 + BBR_RS15875 0.13 +0.4 1,324,767 + BBR_RS15875 0.13 +0.2 1,324,902 + BBR_RS15875 0.30 -1.3 1,324,926 + BBR_RS15875 0.33 +0.2 1,324,927 - BBR_RS15875 0.33 +0.2 1,324,933 - BBR_RS15875 0.34 -0.2 1,324,959 - BBR_RS15875 0.37 -0.8 1,324,998 + BBR_RS15875 0.42 -0.2 1,324,998 + BBR_RS15875 0.42 +0.4 1,325,006 - BBR_RS15875 0.43 +0.1 1,325,006 - BBR_RS15875 0.43 -1.1 1,325,006 - BBR_RS15875 0.43 -0.1 1,325,006 - BBR_RS15875 0.43 +0.1 1,325,006 - BBR_RS15875 0.43 -1.5 1,325,006 - BBR_RS15875 0.43 +0.3 1,325,011 - BBR_RS15875 0.43 +1.1 1,325,011 - BBR_RS15875 0.43 +0.5 1,325,026 + BBR_RS15875 0.45 +0.2 1,325,035 + BBR_RS15875 0.46 -0.0 1,325,081 - BBR_RS15875 0.52 +0.1 1,325,116 - BBR_RS15875 0.56 +1.1 1,325,132 - BBR_RS15875 0.58 -1.4 1,325,202 - BBR_RS15875 0.67 +0.8 1,325,232 + BBR_RS15875 0.71 +0.5 1,325,303 + BBR_RS15875 0.79 +0.2 1,325,389 + +0.2 1,325,395 + +0.3 1,325,418 - -0.1 1,325,579 + +0.2 1,325,634 + -0.3 1,325,720 - BBR_RS15880 0.35 -0.6 1,325,737 + BBR_RS15880 0.42 -1.1 1,325,791 + BBR_RS15880 0.63 -0.2 1,325,804 - BBR_RS15880 0.68 -0.3 1,325,823 + BBR_RS15880 0.75 +0.1 1,325,998 + +0.3 1,326,000 - -0.5 1,326,000 - +0.4 1,326,021 - +1.0 1,326,034 + +1.4 1,326,132 + -1.4 1,326,172 - -0.2 1,326,172 - -0.5 1,326,204 - +1.1 1,326,412 + +0.5 1,326,435 + +0.0 1,326,459 - -0.4 1,326,739 + BBR_RS20955 0.60 -0.0 1,326,739 + BBR_RS20955 0.60 -1.7 1,326,807 + +0.2 1,326,815 - +1.0 1,326,864 + +0.2 1,326,866 + +0.2 1,326,866 + -0.9 1,326,874 - +1.4 1,326,874 - -1.3
Or see this region's nucleotide sequence