Experiment: Recovery broth with Lacidipine 0.4 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS15295 and BBR_RS20900 are separated by 359 nucleotides BBR_RS20900 and BBR_RS15300 are separated by 39 nucleotides BBR_RS15300 and BBR_RS15305 are separated by 64 nucleotides
BBR_RS15295: BBR_RS15295 - Cof-type HAD-IIB family hydrolase, at 1,204,987 to 1,205,832
_RS15295
BBR_RS20900: BBR_RS20900 - hypothetical protein, at 1,206,192 to 1,206,434
_RS20900
BBR_RS15300: BBR_RS15300 - peroxiredoxin, at 1,206,474 to 1,207,061
_RS15300
BBR_RS15305: BBR_RS15305 - hypothetical protein, at 1,207,126 to 1,207,950
_RS15305
Position (kb)
1206
1207
1208 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1205.531 kb on + strand, within BBR_RS15295 at 1205.544 kb on + strand, within BBR_RS15295 at 1205.550 kb on - strand, within BBR_RS15295 at 1205.643 kb on + strand, within BBR_RS15295 at 1205.725 kb on + strand, within BBR_RS15295 at 1205.733 kb on - strand, within BBR_RS15295 at 1205.757 kb on - strand at 1205.757 kb on - strand at 1205.766 kb on + strand at 1205.766 kb on - strand at 1206.091 kb on - strand at 1206.446 kb on + strand at 1206.475 kb on - strand at 1206.544 kb on - strand, within BBR_RS15300 at 1206.588 kb on - strand, within BBR_RS15300 at 1206.606 kb on - strand, within BBR_RS15300 at 1206.606 kb on - strand, within BBR_RS15300 at 1206.667 kb on + strand, within BBR_RS15300 at 1206.679 kb on + strand, within BBR_RS15300 at 1206.739 kb on + strand, within BBR_RS15300 at 1206.834 kb on + strand, within BBR_RS15300 at 1206.851 kb on - strand, within BBR_RS15300 at 1206.899 kb on - strand, within BBR_RS15300 at 1206.901 kb on + strand, within BBR_RS15300 at 1206.931 kb on - strand, within BBR_RS15300 at 1206.983 kb on + strand, within BBR_RS15300 at 1207.054 kb on + strand at 1207.062 kb on - strand at 1207.067 kb on - strand at 1207.083 kb on + strand at 1207.151 kb on - strand at 1207.171 kb on - strand at 1207.293 kb on + strand, within BBR_RS15305 at 1207.328 kb on - strand, within BBR_RS15305 at 1207.371 kb on - strand, within BBR_RS15305 at 1207.379 kb on + strand, within BBR_RS15305 at 1207.392 kb on - strand, within BBR_RS15305 at 1207.419 kb on + strand, within BBR_RS15305 at 1207.419 kb on + strand, within BBR_RS15305 at 1207.429 kb on + strand, within BBR_RS15305 at 1207.464 kb on + strand, within BBR_RS15305 at 1207.584 kb on + strand, within BBR_RS15305 at 1207.592 kb on - strand, within BBR_RS15305 at 1207.594 kb on + strand, within BBR_RS15305 at 1207.777 kb on - strand, within BBR_RS15305 at 1207.880 kb on + strand at 1207.992 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Lacidipine 0.4 mM remove 1,205,531 + BBR_RS15295 0.64 +0.4 1,205,544 + BBR_RS15295 0.66 -1.0 1,205,550 - BBR_RS15295 0.67 +0.4 1,205,643 + BBR_RS15295 0.78 +0.7 1,205,725 + BBR_RS15295 0.87 -0.2 1,205,733 - BBR_RS15295 0.88 +0.9 1,205,757 - -0.3 1,205,757 - -0.5 1,205,766 + -0.2 1,205,766 - -0.4 1,206,091 - -0.7 1,206,446 + +1.3 1,206,475 - +0.3 1,206,544 - BBR_RS15300 0.12 +0.5 1,206,588 - BBR_RS15300 0.19 -0.3 1,206,606 - BBR_RS15300 0.22 -0.0 1,206,606 - BBR_RS15300 0.22 +0.1 1,206,667 + BBR_RS15300 0.33 +1.8 1,206,679 + BBR_RS15300 0.35 -1.6 1,206,739 + BBR_RS15300 0.45 +0.3 1,206,834 + BBR_RS15300 0.61 -1.9 1,206,851 - BBR_RS15300 0.64 +0.5 1,206,899 - BBR_RS15300 0.72 -1.4 1,206,901 + BBR_RS15300 0.73 -0.5 1,206,931 - BBR_RS15300 0.78 -0.2 1,206,983 + BBR_RS15300 0.87 -0.9 1,207,054 + +0.3 1,207,062 - +1.8 1,207,067 - -1.3 1,207,083 + +0.2 1,207,151 - -0.4 1,207,171 - -1.4 1,207,293 + BBR_RS15305 0.20 +0.3 1,207,328 - BBR_RS15305 0.24 +0.5 1,207,371 - BBR_RS15305 0.30 +0.2 1,207,379 + BBR_RS15305 0.31 +0.4 1,207,392 - BBR_RS15305 0.32 +0.4 1,207,419 + BBR_RS15305 0.36 -0.4 1,207,419 + BBR_RS15305 0.36 -0.1 1,207,429 + BBR_RS15305 0.37 +0.2 1,207,464 + BBR_RS15305 0.41 -0.0 1,207,584 + BBR_RS15305 0.56 +0.2 1,207,592 - BBR_RS15305 0.56 -1.1 1,207,594 + BBR_RS15305 0.57 -0.9 1,207,777 - BBR_RS15305 0.79 +0.9 1,207,880 + +0.9 1,207,992 + +0.8
Or see this region's nucleotide sequence