Experiment: Recovery broth with Lacidipine 0.4 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS15080 and BBR_RS15085 are separated by 34 nucleotides BBR_RS15085 and BBR_RS15090 are separated by 151 nucleotides BBR_RS15090 and BBR_RS20880 are separated by 59 nucleotides BBR_RS20880 and BBR_RS15095 overlap by 36 nucleotides
BBR_RS15080: BBR_RS15080 - chorismate synthase, at 1,151,320 to 1,152,507
_RS15080
BBR_RS15085: BBR_RS15085 - 3-dehydroquinate synthase, at 1,152,542 to 1,154,164
_RS15085
BBR_RS15090: BBR_RS15090 - type II 3-dehydroquinate dehydratase, at 1,154,316 to 1,154,762
_RS15090
BBR_RS20880: BBR_RS20880 - CTP synthetase, at 1,154,822 to 1,154,917
_RS20880
BBR_RS15095: BBR_RS15095 - CTP synthase, at 1,154,882 to 1,156,546
_RS15095
Position (kb)
1152
1153
1154
1155 Strain fitness (log2 ratio)
-2
-1
0
1 at 1151.833 kb on + strand, within BBR_RS15080 at 1151.841 kb on - strand, within BBR_RS15080 at 1151.843 kb on + strand, within BBR_RS15080 at 1152.090 kb on + strand, within BBR_RS15080 at 1152.275 kb on + strand, within BBR_RS15080 at 1152.353 kb on + strand, within BBR_RS15080 at 1152.369 kb on - strand, within BBR_RS15080 at 1152.416 kb on - strand at 1152.464 kb on + strand at 1152.464 kb on + strand at 1152.464 kb on + strand at 1152.522 kb on + strand at 1152.523 kb on - strand at 1152.530 kb on + strand at 1152.530 kb on + strand at 1152.538 kb on - strand at 1152.859 kb on + strand, within BBR_RS15085 at 1152.993 kb on + strand, within BBR_RS15085 at 1153.016 kb on - strand, within BBR_RS15085 at 1154.191 kb on + strand at 1154.193 kb on + strand at 1154.201 kb on - strand at 1154.219 kb on - strand at 1154.228 kb on - strand at 1154.229 kb on - strand at 1154.239 kb on + strand at 1154.273 kb on - strand at 1154.292 kb on - strand at 1154.521 kb on + strand, within BBR_RS15090 at 1154.706 kb on + strand, within BBR_RS15090
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Lacidipine 0.4 mM remove 1,151,833 + BBR_RS15080 0.43 -1.2 1,151,841 - BBR_RS15080 0.44 -0.6 1,151,843 + BBR_RS15080 0.44 +0.8 1,152,090 + BBR_RS15080 0.65 -1.0 1,152,275 + BBR_RS15080 0.80 -0.8 1,152,353 + BBR_RS15080 0.87 -0.0 1,152,369 - BBR_RS15080 0.88 -0.8 1,152,416 - -0.8 1,152,464 + -1.4 1,152,464 + +0.5 1,152,464 + -0.3 1,152,522 + -0.4 1,152,523 - -0.4 1,152,530 + -0.1 1,152,530 + +0.0 1,152,538 - +0.6 1,152,859 + BBR_RS15085 0.20 -0.2 1,152,993 + BBR_RS15085 0.28 -1.1 1,153,016 - BBR_RS15085 0.29 -1.5 1,154,191 + +0.3 1,154,193 + -0.6 1,154,201 - -0.4 1,154,219 - -1.1 1,154,228 - -0.4 1,154,229 - +0.1 1,154,239 + -0.4 1,154,273 - +0.2 1,154,292 - -0.8 1,154,521 + BBR_RS15090 0.46 -1.9 1,154,706 + BBR_RS15090 0.87 -0.4
Or see this region's nucleotide sequence