Experiment: Recovery broth with Lacidipine 0.4 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS14610 and BBR_RS14615 are separated by 21 nucleotides BBR_RS14615 and BBR_RS14620 are separated by 81 nucleotides
BBR_RS14610: BBR_RS14610 - DNA-binding response regulator, at 1,052,467 to 1,053,273
_RS14610
BBR_RS14615: BBR_RS14615 - histidine kinase, at 1,053,295 to 1,055,763
_RS14615
BBR_RS14620: BBR_RS14620 - proline iminopeptidase, at 1,055,845 to 1,057,323
_RS14620
Position (kb)
1053
1054
1055
1056 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1052.364 kb on + strand at 1052.465 kb on + strand at 1052.470 kb on + strand at 1052.502 kb on - strand at 1052.520 kb on - strand at 1052.544 kb on - strand at 1052.735 kb on + strand, within BBR_RS14610 at 1052.908 kb on - strand, within BBR_RS14610 at 1052.919 kb on + strand, within BBR_RS14610 at 1053.013 kb on + strand, within BBR_RS14610 at 1053.013 kb on + strand, within BBR_RS14610 at 1053.014 kb on - strand, within BBR_RS14610 at 1053.194 kb on + strand at 1053.228 kb on - strand at 1053.269 kb on + strand at 1053.277 kb on + strand at 1053.284 kb on - strand at 1053.318 kb on + strand at 1053.326 kb on - strand at 1053.326 kb on - strand at 1053.366 kb on - strand at 1053.368 kb on - strand at 1053.401 kb on + strand at 1053.559 kb on + strand, within BBR_RS14615 at 1053.559 kb on + strand, within BBR_RS14615 at 1054.002 kb on + strand, within BBR_RS14615 at 1054.010 kb on - strand, within BBR_RS14615 at 1054.021 kb on + strand, within BBR_RS14615 at 1054.033 kb on + strand, within BBR_RS14615 at 1054.088 kb on + strand, within BBR_RS14615 at 1054.158 kb on + strand, within BBR_RS14615 at 1054.160 kb on + strand, within BBR_RS14615 at 1054.165 kb on + strand, within BBR_RS14615 at 1054.168 kb on - strand, within BBR_RS14615 at 1054.168 kb on - strand, within BBR_RS14615 at 1054.168 kb on - strand, within BBR_RS14615 at 1054.168 kb on - strand, within BBR_RS14615 at 1054.173 kb on - strand, within BBR_RS14615 at 1054.173 kb on - strand, within BBR_RS14615 at 1054.175 kb on + strand, within BBR_RS14615 at 1054.188 kb on - strand, within BBR_RS14615 at 1054.235 kb on + strand, within BBR_RS14615 at 1054.235 kb on + strand, within BBR_RS14615 at 1054.413 kb on - strand, within BBR_RS14615 at 1054.565 kb on - strand, within BBR_RS14615 at 1054.603 kb on + strand, within BBR_RS14615 at 1054.623 kb on + strand, within BBR_RS14615 at 1054.698 kb on - strand, within BBR_RS14615 at 1054.707 kb on - strand, within BBR_RS14615 at 1054.707 kb on - strand, within BBR_RS14615 at 1054.709 kb on + strand, within BBR_RS14615 at 1054.774 kb on - strand, within BBR_RS14615 at 1054.865 kb on + strand, within BBR_RS14615 at 1054.929 kb on + strand, within BBR_RS14615 at 1054.983 kb on + strand, within BBR_RS14615 at 1055.123 kb on + strand, within BBR_RS14615 at 1055.142 kb on + strand, within BBR_RS14615 at 1055.165 kb on + strand, within BBR_RS14615 at 1055.184 kb on + strand, within BBR_RS14615 at 1055.246 kb on + strand, within BBR_RS14615 at 1055.249 kb on - strand, within BBR_RS14615 at 1055.258 kb on + strand, within BBR_RS14615 at 1055.309 kb on + strand, within BBR_RS14615 at 1055.364 kb on - strand, within BBR_RS14615 at 1055.382 kb on - strand, within BBR_RS14615 at 1055.428 kb on + strand, within BBR_RS14615 at 1055.444 kb on + strand, within BBR_RS14615 at 1055.593 kb on + strand at 1055.688 kb on - strand at 1055.688 kb on - strand at 1055.705 kb on + strand at 1055.715 kb on - strand at 1055.727 kb on + strand at 1055.738 kb on + strand at 1055.752 kb on - strand at 1055.752 kb on - strand at 1055.786 kb on - strand at 1055.804 kb on + strand at 1055.812 kb on - strand at 1055.875 kb on + strand at 1055.877 kb on - strand at 1055.965 kb on + strand at 1055.965 kb on + strand at 1055.965 kb on + strand at 1055.975 kb on - strand at 1055.975 kb on - strand at 1055.989 kb on + strand at 1056.058 kb on + strand, within BBR_RS14620 at 1056.066 kb on - strand, within BBR_RS14620 at 1056.066 kb on - strand, within BBR_RS14620 at 1056.066 kb on - strand, within BBR_RS14620 at 1056.066 kb on - strand, within BBR_RS14620 at 1056.066 kb on - strand, within BBR_RS14620 at 1056.118 kb on + strand, within BBR_RS14620 at 1056.130 kb on - strand, within BBR_RS14620 at 1056.137 kb on + strand, within BBR_RS14620 at 1056.137 kb on + strand, within BBR_RS14620 at 1056.182 kb on - strand, within BBR_RS14620 at 1056.343 kb on + strand, within BBR_RS14620 at 1056.344 kb on + strand, within BBR_RS14620 at 1056.439 kb on + strand, within BBR_RS14620 at 1056.445 kb on + strand, within BBR_RS14620 at 1056.476 kb on - strand, within BBR_RS14620 at 1056.476 kb on - strand, within BBR_RS14620 at 1056.537 kb on + strand, within BBR_RS14620 at 1056.659 kb on - strand, within BBR_RS14620 at 1056.672 kb on + strand, within BBR_RS14620 at 1056.708 kb on - strand, within BBR_RS14620 at 1056.711 kb on - strand, within BBR_RS14620 at 1056.760 kb on + strand, within BBR_RS14620
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Lacidipine 0.4 mM remove 1,052,364 + -0.3 1,052,465 + +0.3 1,052,470 + -1.2 1,052,502 - -0.2 1,052,520 - -0.7 1,052,544 - +0.4 1,052,735 + BBR_RS14610 0.33 -0.1 1,052,908 - BBR_RS14610 0.55 +1.6 1,052,919 + BBR_RS14610 0.56 -0.1 1,053,013 + BBR_RS14610 0.68 -1.3 1,053,013 + BBR_RS14610 0.68 -0.6 1,053,014 - BBR_RS14610 0.68 -0.5 1,053,194 + +0.3 1,053,228 - +0.7 1,053,269 + +0.6 1,053,277 + -0.2 1,053,284 - +0.6 1,053,318 + -0.3 1,053,326 - +1.0 1,053,326 - -0.4 1,053,366 - -1.1 1,053,368 - +0.5 1,053,401 + -1.2 1,053,559 + BBR_RS14615 0.11 -3.0 1,053,559 + BBR_RS14615 0.11 -1.2 1,054,002 + BBR_RS14615 0.29 -0.3 1,054,010 - BBR_RS14615 0.29 -1.1 1,054,021 + BBR_RS14615 0.29 -1.4 1,054,033 + BBR_RS14615 0.30 -1.9 1,054,088 + BBR_RS14615 0.32 -0.2 1,054,158 + BBR_RS14615 0.35 +0.0 1,054,160 + BBR_RS14615 0.35 -0.4 1,054,165 + BBR_RS14615 0.35 +0.7 1,054,168 - BBR_RS14615 0.35 -0.5 1,054,168 - BBR_RS14615 0.35 +0.3 1,054,168 - BBR_RS14615 0.35 +0.9 1,054,168 - BBR_RS14615 0.35 -1.0 1,054,173 - BBR_RS14615 0.36 -0.6 1,054,173 - BBR_RS14615 0.36 +0.7 1,054,175 + BBR_RS14615 0.36 +0.5 1,054,188 - BBR_RS14615 0.36 -1.2 1,054,235 + BBR_RS14615 0.38 -0.9 1,054,235 + BBR_RS14615 0.38 -0.5 1,054,413 - BBR_RS14615 0.45 -1.7 1,054,565 - BBR_RS14615 0.51 -0.2 1,054,603 + BBR_RS14615 0.53 -0.7 1,054,623 + BBR_RS14615 0.54 +0.7 1,054,698 - BBR_RS14615 0.57 +0.6 1,054,707 - BBR_RS14615 0.57 -1.5 1,054,707 - BBR_RS14615 0.57 -1.8 1,054,709 + BBR_RS14615 0.57 -0.3 1,054,774 - BBR_RS14615 0.60 -0.4 1,054,865 + BBR_RS14615 0.64 -0.5 1,054,929 + BBR_RS14615 0.66 -0.2 1,054,983 + BBR_RS14615 0.68 +0.4 1,055,123 + BBR_RS14615 0.74 -0.6 1,055,142 + BBR_RS14615 0.75 -0.1 1,055,165 + BBR_RS14615 0.76 +0.7 1,055,184 + BBR_RS14615 0.77 -1.0 1,055,246 + BBR_RS14615 0.79 +0.2 1,055,249 - BBR_RS14615 0.79 -0.7 1,055,258 + BBR_RS14615 0.80 -0.4 1,055,309 + BBR_RS14615 0.82 +1.1 1,055,364 - BBR_RS14615 0.84 +0.3 1,055,382 - BBR_RS14615 0.85 -0.1 1,055,428 + BBR_RS14615 0.86 -0.3 1,055,444 + BBR_RS14615 0.87 -0.1 1,055,593 + +1.6 1,055,688 - -0.1 1,055,688 - -1.0 1,055,705 + -0.3 1,055,715 - -0.5 1,055,727 + -0.9 1,055,738 + -0.2 1,055,752 - +0.4 1,055,752 - +0.2 1,055,786 - +0.5 1,055,804 + -0.2 1,055,812 - +0.1 1,055,875 + +0.3 1,055,877 - -0.7 1,055,965 + +0.5 1,055,965 + -0.0 1,055,965 + +0.9 1,055,975 - -1.0 1,055,975 - -0.7 1,055,989 + +0.3 1,056,058 + BBR_RS14620 0.14 -0.1 1,056,066 - BBR_RS14620 0.15 -0.5 1,056,066 - BBR_RS14620 0.15 -0.4 1,056,066 - BBR_RS14620 0.15 -0.5 1,056,066 - BBR_RS14620 0.15 -0.4 1,056,066 - BBR_RS14620 0.15 -0.2 1,056,118 + BBR_RS14620 0.18 +0.0 1,056,130 - BBR_RS14620 0.19 -1.5 1,056,137 + BBR_RS14620 0.20 +0.3 1,056,137 + BBR_RS14620 0.20 +0.2 1,056,182 - BBR_RS14620 0.23 +0.7 1,056,343 + BBR_RS14620 0.34 +0.6 1,056,344 + BBR_RS14620 0.34 +0.4 1,056,439 + BBR_RS14620 0.40 +1.0 1,056,445 + BBR_RS14620 0.41 +0.4 1,056,476 - BBR_RS14620 0.43 -1.5 1,056,476 - BBR_RS14620 0.43 +0.1 1,056,537 + BBR_RS14620 0.47 +0.7 1,056,659 - BBR_RS14620 0.55 -0.7 1,056,672 + BBR_RS14620 0.56 +0.3 1,056,708 - BBR_RS14620 0.58 +0.2 1,056,711 - BBR_RS14620 0.59 -0.6 1,056,760 + BBR_RS14620 0.62 -1.2
Or see this region's nucleotide sequence