Experiment: Recovery broth with Lacidipine 0.4 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS14320 and BBR_RS14325 are separated by 42 nucleotides BBR_RS14325 and BBR_RS14330 are separated by 73 nucleotides BBR_RS14330 and BBR_RS14335 are separated by 137 nucleotides BBR_RS14335 and BBR_RS14340 are separated by 148 nucleotides BBR_RS14340 and BBR_RS14345 are separated by 3 nucleotides
BBR_RS14320: BBR_RS14320 - Nif3-like dinuclear metal center hexameric protein, at 989,045 to 989,965
_RS14320
BBR_RS14325: BBR_RS14325 - NUDIX hydrolase, at 990,008 to 990,682
_RS14325
BBR_RS14330: BBR_RS14330 - glycogen debranching enzyme GlgX, at 990,756 to 992,867
_RS14330
BBR_RS14335: BBR_RS14335 - hypothetical protein, at 993,005 to 993,205
_RS14335
BBR_RS14340: BBR_RS14340 - helix-turn-helix domain-containing protein, at 993,354 to 993,839
_RS14340
BBR_RS14345: BBR_RS14345 - hypothetical protein, at 993,843 to 994,493
_RS14345
Position (kb) 990
991
992
993 Strain fitness (log2 ratio) -2
-1
0
1
2 at 989.757 kb on + strand, within BBR_RS14320 at 989.759 kb on - strand, within BBR_RS14320 at 989.791 kb on - strand, within BBR_RS14320 at 989.811 kb on - strand, within BBR_RS14320 at 989.850 kb on + strand, within BBR_RS14320 at 989.850 kb on + strand, within BBR_RS14320 at 989.881 kb on + strand at 989.941 kb on + strand at 989.941 kb on + strand at 989.941 kb on + strand at 989.949 kb on - strand at 989.954 kb on + strand at 989.954 kb on + strand at 989.963 kb on - strand at 989.978 kb on - strand at 989.980 kb on - strand at 990.008 kb on - strand at 990.084 kb on + strand, within BBR_RS14325 at 990.114 kb on + strand, within BBR_RS14325 at 990.114 kb on + strand, within BBR_RS14325 at 990.165 kb on - strand, within BBR_RS14325 at 990.204 kb on + strand, within BBR_RS14325 at 990.260 kb on + strand, within BBR_RS14325 at 990.260 kb on + strand, within BBR_RS14325 at 990.269 kb on - strand, within BBR_RS14325 at 990.275 kb on - strand, within BBR_RS14325 at 990.301 kb on - strand, within BBR_RS14325 at 990.309 kb on + strand, within BBR_RS14325 at 990.372 kb on + strand, within BBR_RS14325 at 990.411 kb on - strand, within BBR_RS14325 at 990.444 kb on + strand, within BBR_RS14325 at 990.475 kb on + strand, within BBR_RS14325 at 990.475 kb on + strand, within BBR_RS14325 at 990.489 kb on + strand, within BBR_RS14325 at 990.561 kb on - strand, within BBR_RS14325 at 990.943 kb on - strand at 990.960 kb on + strand at 991.420 kb on - strand, within BBR_RS14330 at 991.487 kb on + strand, within BBR_RS14330 at 991.825 kb on - strand, within BBR_RS14330 at 991.989 kb on - strand, within BBR_RS14330 at 992.029 kb on + strand, within BBR_RS14330 at 992.037 kb on - strand, within BBR_RS14330 at 992.047 kb on - strand, within BBR_RS14330 at 992.078 kb on + strand, within BBR_RS14330 at 992.284 kb on - strand, within BBR_RS14330 at 992.546 kb on - strand, within BBR_RS14330 at 992.573 kb on + strand, within BBR_RS14330 at 992.678 kb on + strand at 992.788 kb on + strand at 992.910 kb on + strand at 992.917 kb on + strand at 992.917 kb on + strand at 992.956 kb on - strand at 992.962 kb on + strand at 993.000 kb on + strand at 993.101 kb on + strand, within BBR_RS14335 at 993.101 kb on + strand, within BBR_RS14335 at 993.125 kb on - strand, within BBR_RS14335 at 993.132 kb on + strand, within BBR_RS14335 at 993.132 kb on + strand, within BBR_RS14335 at 993.140 kb on - strand, within BBR_RS14335 at 993.181 kb on + strand, within BBR_RS14335 at 993.189 kb on - strand at 993.286 kb on + strand at 993.294 kb on - strand at 993.304 kb on - strand at 993.371 kb on + strand at 993.390 kb on + strand at 993.519 kb on - strand, within BBR_RS14340 at 993.732 kb on + strand, within BBR_RS14340 at 993.742 kb on + strand, within BBR_RS14340 at 993.760 kb on - strand, within BBR_RS14340 at 993.760 kb on - strand, within BBR_RS14340 at 993.809 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Lacidipine 0.4 mM remove 989,757 + BBR_RS14320 0.77 +1.2 989,759 - BBR_RS14320 0.78 +0.0 989,791 - BBR_RS14320 0.81 +0.0 989,811 - BBR_RS14320 0.83 +0.1 989,850 + BBR_RS14320 0.87 -0.7 989,850 + BBR_RS14320 0.87 -0.6 989,881 + +0.6 989,941 + -0.6 989,941 + -1.4 989,941 + +0.5 989,949 - +0.2 989,954 + +0.0 989,954 + +0.0 989,963 - -0.8 989,978 - +2.4 989,980 - +0.2 990,008 - +0.6 990,084 + BBR_RS14325 0.11 -0.2 990,114 + BBR_RS14325 0.16 +1.1 990,114 + BBR_RS14325 0.16 -0.2 990,165 - BBR_RS14325 0.23 +1.2 990,204 + BBR_RS14325 0.29 +0.4 990,260 + BBR_RS14325 0.37 +0.6 990,260 + BBR_RS14325 0.37 -1.4 990,269 - BBR_RS14325 0.39 +1.4 990,275 - BBR_RS14325 0.40 +1.4 990,301 - BBR_RS14325 0.43 -0.4 990,309 + BBR_RS14325 0.45 -2.0 990,372 + BBR_RS14325 0.54 +1.5 990,411 - BBR_RS14325 0.60 -0.1 990,444 + BBR_RS14325 0.65 -0.2 990,475 + BBR_RS14325 0.69 -0.4 990,475 + BBR_RS14325 0.69 -1.0 990,489 + BBR_RS14325 0.71 +1.6 990,561 - BBR_RS14325 0.82 +0.1 990,943 - +0.8 990,960 + +2.1 991,420 - BBR_RS14330 0.31 +2.4 991,487 + BBR_RS14330 0.35 +0.6 991,825 - BBR_RS14330 0.51 +1.8 991,989 - BBR_RS14330 0.58 +1.8 992,029 + BBR_RS14330 0.60 +1.1 992,037 - BBR_RS14330 0.61 +1.8 992,047 - BBR_RS14330 0.61 -0.9 992,078 + BBR_RS14330 0.63 +1.4 992,284 - BBR_RS14330 0.72 -0.3 992,546 - BBR_RS14330 0.85 -1.1 992,573 + BBR_RS14330 0.86 +0.1 992,678 + +0.2 992,788 + +2.4 992,910 + -0.3 992,917 + -0.2 992,917 + +0.5 992,956 - +0.2 992,962 + +1.3 993,000 + -0.8 993,101 + BBR_RS14335 0.48 +0.4 993,101 + BBR_RS14335 0.48 -1.4 993,125 - BBR_RS14335 0.60 +0.0 993,132 + BBR_RS14335 0.63 +1.4 993,132 + BBR_RS14335 0.63 +0.2 993,140 - BBR_RS14335 0.67 +0.1 993,181 + BBR_RS14335 0.88 -0.5 993,189 - +0.1 993,286 + +0.5 993,294 - +0.1 993,304 - -0.1 993,371 + +0.0 993,390 + +0.3 993,519 - BBR_RS14340 0.34 +0.2 993,732 + BBR_RS14340 0.78 -0.1 993,742 + BBR_RS14340 0.80 -1.5 993,760 - BBR_RS14340 0.84 -0.3 993,760 - BBR_RS14340 0.84 +0.2 993,809 - -0.7
Or see this region's nucleotide sequence