Experiment: Recovery broth with Lacidipine 0.4 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS12350 and BBR_RS12355 are separated by 159 nucleotides BBR_RS12355 and BBR_RS12360 are separated by 170 nucleotides BBR_RS12360 and BBR_RS12365 are separated by 89 nucleotides
BBR_RS12350: BBR_RS12350 - acetolactate synthase large subunit, at 522,036 to 524,000
_RS12350
BBR_RS12355: BBR_RS12355 - ribonuclease III, at 524,160 to 524,903
_RS12355
BBR_RS12360: BBR_RS12360 - 50S ribosomal protein L32, at 525,074 to 525,268
_RS12360
BBR_RS12365: BBR_RS12365 - DUF177 domain-containing protein, at 525,358 to 525,978
_RS12365
Position (kb)
524
525 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 523.175 kb on - strand, within BBR_RS12350 at 523.177 kb on + strand, within BBR_RS12350 at 523.177 kb on + strand, within BBR_RS12350 at 523.209 kb on + strand, within BBR_RS12350 at 523.214 kb on + strand, within BBR_RS12350 at 523.214 kb on + strand, within BBR_RS12350 at 523.215 kb on - strand, within BBR_RS12350 at 523.215 kb on - strand, within BBR_RS12350 at 523.243 kb on + strand, within BBR_RS12350 at 523.243 kb on + strand, within BBR_RS12350 at 523.243 kb on + strand, within BBR_RS12350 at 523.243 kb on + strand, within BBR_RS12350 at 523.243 kb on + strand, within BBR_RS12350 at 523.243 kb on + strand, within BBR_RS12350 at 523.244 kb on - strand, within BBR_RS12350 at 523.244 kb on - strand, within BBR_RS12350 at 523.245 kb on - strand, within BBR_RS12350 at 523.251 kb on - strand, within BBR_RS12350 at 523.251 kb on - strand, within BBR_RS12350 at 523.251 kb on - strand, within BBR_RS12350 at 523.262 kb on + strand, within BBR_RS12350 at 523.268 kb on - strand, within BBR_RS12350 at 523.280 kb on - strand, within BBR_RS12350 at 523.290 kb on + strand, within BBR_RS12350 at 523.290 kb on + strand, within BBR_RS12350 at 523.303 kb on + strand, within BBR_RS12350 at 523.303 kb on - strand, within BBR_RS12350 at 523.340 kb on + strand, within BBR_RS12350 at 523.348 kb on - strand, within BBR_RS12350 at 523.402 kb on + strand, within BBR_RS12350 at 523.402 kb on + strand, within BBR_RS12350 at 523.404 kb on + strand, within BBR_RS12350 at 523.405 kb on - strand, within BBR_RS12350 at 523.407 kb on + strand, within BBR_RS12350 at 523.407 kb on + strand, within BBR_RS12350 at 523.407 kb on + strand, within BBR_RS12350 at 523.412 kb on + strand, within BBR_RS12350 at 523.429 kb on + strand, within BBR_RS12350 at 523.437 kb on - strand, within BBR_RS12350 at 523.437 kb on - strand, within BBR_RS12350 at 523.462 kb on - strand, within BBR_RS12350 at 523.468 kb on + strand, within BBR_RS12350 at 523.487 kb on + strand, within BBR_RS12350 at 523.521 kb on + strand, within BBR_RS12350 at 523.549 kb on + strand, within BBR_RS12350 at 523.549 kb on + strand, within BBR_RS12350 at 523.557 kb on - strand, within BBR_RS12350 at 523.562 kb on - strand, within BBR_RS12350 at 523.563 kb on - strand, within BBR_RS12350 at 523.563 kb on - strand, within BBR_RS12350 at 523.577 kb on + strand, within BBR_RS12350 at 523.594 kb on + strand, within BBR_RS12350 at 523.619 kb on - strand, within BBR_RS12350 at 523.621 kb on + strand, within BBR_RS12350 at 523.631 kb on - strand, within BBR_RS12350 at 523.631 kb on - strand, within BBR_RS12350 at 523.642 kb on + strand, within BBR_RS12350 at 523.650 kb on - strand, within BBR_RS12350 at 523.668 kb on + strand, within BBR_RS12350 at 523.691 kb on + strand, within BBR_RS12350 at 523.691 kb on + strand, within BBR_RS12350 at 523.698 kb on + strand, within BBR_RS12350 at 523.699 kb on - strand, within BBR_RS12350 at 523.706 kb on + strand, within BBR_RS12350 at 523.716 kb on + strand, within BBR_RS12350 at 523.719 kb on - strand, within BBR_RS12350 at 523.731 kb on - strand, within BBR_RS12350 at 523.736 kb on - strand, within BBR_RS12350 at 523.740 kb on + strand, within BBR_RS12350 at 523.811 kb on - strand at 523.816 kb on + strand at 523.837 kb on - strand at 523.837 kb on - strand at 523.849 kb on + strand at 523.903 kb on + strand at 523.905 kb on + strand at 523.940 kb on - strand at 523.974 kb on - strand at 523.984 kb on + strand at 523.992 kb on - strand at 524.067 kb on + strand at 524.124 kb on + strand at 524.124 kb on - strand at 524.136 kb on - strand at 524.165 kb on - strand at 524.192 kb on - strand at 524.324 kb on + strand, within BBR_RS12355 at 524.327 kb on - strand, within BBR_RS12355 at 524.332 kb on - strand, within BBR_RS12355 at 524.347 kb on + strand, within BBR_RS12355 at 524.355 kb on - strand, within BBR_RS12355 at 524.355 kb on - strand, within BBR_RS12355 at 524.465 kb on + strand, within BBR_RS12355 at 524.468 kb on - strand, within BBR_RS12355 at 524.552 kb on - strand, within BBR_RS12355 at 524.554 kb on + strand, within BBR_RS12355 at 524.621 kb on - strand, within BBR_RS12355 at 524.654 kb on - strand, within BBR_RS12355 at 524.701 kb on - strand, within BBR_RS12355 at 524.789 kb on + strand, within BBR_RS12355 at 524.810 kb on + strand, within BBR_RS12355 at 524.836 kb on + strand at 524.838 kb on + strand at 524.932 kb on - strand at 524.936 kb on + strand at 524.943 kb on + strand at 524.944 kb on - strand at 524.944 kb on - strand at 524.948 kb on + strand at 524.948 kb on + strand at 524.963 kb on - strand at 524.973 kb on - strand at 524.975 kb on - strand at 524.977 kb on + strand at 524.982 kb on - strand at 524.983 kb on + strand at 525.379 kb on + strand at 525.382 kb on - strand at 525.388 kb on - strand at 525.394 kb on - strand at 525.456 kb on + strand, within BBR_RS12365 at 525.456 kb on + strand, within BBR_RS12365 at 525.458 kb on - strand, within BBR_RS12365 at 525.461 kb on + strand, within BBR_RS12365 at 525.464 kb on - strand, within BBR_RS12365 at 525.475 kb on + strand, within BBR_RS12365 at 525.475 kb on + strand, within BBR_RS12365 at 525.476 kb on - strand, within BBR_RS12365 at 525.478 kb on - strand, within BBR_RS12365 at 525.480 kb on + strand, within BBR_RS12365 at 525.488 kb on - strand, within BBR_RS12365 at 525.488 kb on - strand, within BBR_RS12365 at 525.502 kb on - strand, within BBR_RS12365 at 525.528 kb on - strand, within BBR_RS12365 at 525.529 kb on - strand, within BBR_RS12365 at 525.533 kb on - strand, within BBR_RS12365 at 525.602 kb on - strand, within BBR_RS12365 at 525.612 kb on - strand, within BBR_RS12365 at 525.662 kb on + strand, within BBR_RS12365 at 525.667 kb on + strand, within BBR_RS12365 at 525.675 kb on - strand, within BBR_RS12365 at 525.675 kb on - strand, within BBR_RS12365 at 525.676 kb on - strand, within BBR_RS12365 at 525.716 kb on + strand, within BBR_RS12365 at 525.721 kb on + strand, within BBR_RS12365 at 525.735 kb on + strand, within BBR_RS12365 at 525.743 kb on - strand, within BBR_RS12365 at 525.844 kb on - strand, within BBR_RS12365 at 525.850 kb on - strand, within BBR_RS12365 at 525.854 kb on - strand, within BBR_RS12365 at 525.862 kb on - strand, within BBR_RS12365 at 525.890 kb on - strand, within BBR_RS12365 at 525.896 kb on + strand, within BBR_RS12365
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Lacidipine 0.4 mM remove 523,175 - BBR_RS12350 0.58 +0.0 523,177 + BBR_RS12350 0.58 -0.1 523,177 + BBR_RS12350 0.58 +0.3 523,209 + BBR_RS12350 0.60 -0.0 523,214 + BBR_RS12350 0.60 -0.4 523,214 + BBR_RS12350 0.60 +0.6 523,215 - BBR_RS12350 0.60 +0.1 523,215 - BBR_RS12350 0.60 +0.3 523,243 + BBR_RS12350 0.61 +0.7 523,243 + BBR_RS12350 0.61 -0.5 523,243 + BBR_RS12350 0.61 +0.4 523,243 + BBR_RS12350 0.61 -0.1 523,243 + BBR_RS12350 0.61 +0.5 523,243 + BBR_RS12350 0.61 -0.3 523,244 - BBR_RS12350 0.61 +0.5 523,244 - BBR_RS12350 0.61 +0.6 523,245 - BBR_RS12350 0.62 -1.2 523,251 - BBR_RS12350 0.62 -0.6 523,251 - BBR_RS12350 0.62 +0.5 523,251 - BBR_RS12350 0.62 +0.3 523,262 + BBR_RS12350 0.62 +0.1 523,268 - BBR_RS12350 0.63 +0.2 523,280 - BBR_RS12350 0.63 -0.1 523,290 + BBR_RS12350 0.64 +0.3 523,290 + BBR_RS12350 0.64 +0.2 523,303 + BBR_RS12350 0.64 +0.7 523,303 - BBR_RS12350 0.64 +0.4 523,340 + BBR_RS12350 0.66 +0.2 523,348 - BBR_RS12350 0.67 +0.4 523,402 + BBR_RS12350 0.70 +0.4 523,402 + BBR_RS12350 0.70 +0.2 523,404 + BBR_RS12350 0.70 +0.7 523,405 - BBR_RS12350 0.70 +1.1 523,407 + BBR_RS12350 0.70 -0.4 523,407 + BBR_RS12350 0.70 -1.1 523,407 + BBR_RS12350 0.70 +0.8 523,412 + BBR_RS12350 0.70 -0.5 523,429 + BBR_RS12350 0.71 +1.7 523,437 - BBR_RS12350 0.71 -0.8 523,437 - BBR_RS12350 0.71 -0.4 523,462 - BBR_RS12350 0.73 +0.0 523,468 + BBR_RS12350 0.73 -0.5 523,487 + BBR_RS12350 0.74 +0.2 523,521 + BBR_RS12350 0.76 -0.4 523,549 + BBR_RS12350 0.77 +0.2 523,549 + BBR_RS12350 0.77 +0.2 523,557 - BBR_RS12350 0.77 +0.1 523,562 - BBR_RS12350 0.78 -0.5 523,563 - BBR_RS12350 0.78 -0.2 523,563 - BBR_RS12350 0.78 +0.1 523,577 + BBR_RS12350 0.78 -0.5 523,594 + BBR_RS12350 0.79 +0.0 523,619 - BBR_RS12350 0.81 +0.4 523,621 + BBR_RS12350 0.81 -1.1 523,631 - BBR_RS12350 0.81 +1.2 523,631 - BBR_RS12350 0.81 -0.3 523,642 + BBR_RS12350 0.82 +0.5 523,650 - BBR_RS12350 0.82 +1.2 523,668 + BBR_RS12350 0.83 +0.4 523,691 + BBR_RS12350 0.84 +0.4 523,691 + BBR_RS12350 0.84 +0.8 523,698 + BBR_RS12350 0.85 +1.0 523,699 - BBR_RS12350 0.85 +0.3 523,706 + BBR_RS12350 0.85 +0.2 523,716 + BBR_RS12350 0.85 +0.6 523,719 - BBR_RS12350 0.86 +0.7 523,731 - BBR_RS12350 0.86 -0.1 523,736 - BBR_RS12350 0.87 +0.4 523,740 + BBR_RS12350 0.87 +0.5 523,811 - +0.4 523,816 + +1.7 523,837 - -0.2 523,837 - +0.0 523,849 + +0.2 523,903 + -0.3 523,905 + -1.0 523,940 - -0.7 523,974 - -0.7 523,984 + -0.1 523,992 - +0.3 524,067 + -1.0 524,124 + -0.8 524,124 - +1.7 524,136 - -0.8 524,165 - -1.9 524,192 - -0.3 524,324 + BBR_RS12355 0.22 +2.0 524,327 - BBR_RS12355 0.22 -0.4 524,332 - BBR_RS12355 0.23 +0.1 524,347 + BBR_RS12355 0.25 +2.0 524,355 - BBR_RS12355 0.26 -1.9 524,355 - BBR_RS12355 0.26 -0.7 524,465 + BBR_RS12355 0.41 +0.0 524,468 - BBR_RS12355 0.41 +0.2 524,552 - BBR_RS12355 0.53 +0.9 524,554 + BBR_RS12355 0.53 -0.6 524,621 - BBR_RS12355 0.62 -0.4 524,654 - BBR_RS12355 0.66 -0.5 524,701 - BBR_RS12355 0.73 +0.8 524,789 + BBR_RS12355 0.85 -1.2 524,810 + BBR_RS12355 0.87 -0.5 524,836 + -0.4 524,838 + +1.9 524,932 - +1.0 524,936 + -1.1 524,943 + -0.9 524,944 - +0.3 524,944 - +0.0 524,948 + -0.1 524,948 + -0.6 524,963 - +0.8 524,973 - -0.5 524,975 - +0.4 524,977 + +0.9 524,982 - +0.9 524,983 + +0.1 525,379 + -0.3 525,382 - +1.1 525,388 - +1.2 525,394 - +0.3 525,456 + BBR_RS12365 0.16 -0.0 525,456 + BBR_RS12365 0.16 -1.0 525,458 - BBR_RS12365 0.16 +0.8 525,461 + BBR_RS12365 0.17 +0.8 525,464 - BBR_RS12365 0.17 -0.8 525,475 + BBR_RS12365 0.19 +0.1 525,475 + BBR_RS12365 0.19 -1.2 525,476 - BBR_RS12365 0.19 +1.0 525,478 - BBR_RS12365 0.19 -0.0 525,480 + BBR_RS12365 0.20 +0.0 525,488 - BBR_RS12365 0.21 +0.6 525,488 - BBR_RS12365 0.21 -0.5 525,502 - BBR_RS12365 0.23 +0.3 525,528 - BBR_RS12365 0.27 -0.7 525,529 - BBR_RS12365 0.28 -0.3 525,533 - BBR_RS12365 0.28 -1.4 525,602 - BBR_RS12365 0.39 -1.8 525,612 - BBR_RS12365 0.41 +0.3 525,662 + BBR_RS12365 0.49 -1.2 525,667 + BBR_RS12365 0.50 -0.2 525,675 - BBR_RS12365 0.51 -0.5 525,675 - BBR_RS12365 0.51 +0.6 525,676 - BBR_RS12365 0.51 -0.4 525,716 + BBR_RS12365 0.58 +1.4 525,721 + BBR_RS12365 0.58 +0.9 525,735 + BBR_RS12365 0.61 -1.5 525,743 - BBR_RS12365 0.62 -0.4 525,844 - BBR_RS12365 0.78 -1.5 525,850 - BBR_RS12365 0.79 +0.4 525,854 - BBR_RS12365 0.80 +0.3 525,862 - BBR_RS12365 0.81 +0.3 525,890 - BBR_RS12365 0.86 +1.1 525,896 + BBR_RS12365 0.87 +1.0
Or see this region's nucleotide sequence