Experiment: Varel_Bryant_medium_Glucose with Ethanol 3.33 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0250 and BT0251 overlap by 4 nucleotides BT0251 and BT0252 are separated by 244 nucleotides BT0252 and BT0253 are separated by 113 nucleotides
BT0250: BT0250 - dihydroorotase (DHOase) (NCBI ptt file), at 275,479 to 276,816
BT0250
BT0251: BT0251 - dolichol-phosphate mannosyltransferase (NCBI ptt file), at 276,813 to 277,556
BT0251
BT0252: BT0252 - transcription-repair coupling factor (NCBI ptt file), at 277,801 to 281,184
BT0252
BT0253: BT0253 - hypothetical protein (NCBI ptt file), at 281,298 to 282,143
BT0253
Position (kb)
277
278
279
280
281
282 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 277.603 kb on - strand at 277.618 kb on - strand at 277.801 kb on - strand at 277.801 kb on - strand at 277.801 kb on - strand at 277.891 kb on - strand at 278.292 kb on - strand, within BT0252 at 278.302 kb on + strand, within BT0252 at 278.312 kb on - strand, within BT0252 at 278.332 kb on + strand, within BT0252 at 278.332 kb on + strand, within BT0252 at 278.333 kb on - strand, within BT0252 at 278.658 kb on + strand, within BT0252 at 278.956 kb on - strand, within BT0252 at 278.956 kb on - strand, within BT0252 at 279.119 kb on - strand, within BT0252 at 279.183 kb on + strand, within BT0252 at 279.184 kb on - strand, within BT0252 at 279.268 kb on + strand, within BT0252 at 279.343 kb on - strand, within BT0252 at 279.719 kb on - strand, within BT0252 at 279.908 kb on + strand, within BT0252 at 279.909 kb on - strand, within BT0252 at 279.925 kb on - strand, within BT0252 at 279.957 kb on + strand, within BT0252 at 279.999 kb on - strand, within BT0252 at 280.048 kb on - strand, within BT0252 at 280.258 kb on + strand, within BT0252 at 280.258 kb on + strand, within BT0252 at 280.258 kb on + strand, within BT0252 at 280.258 kb on + strand, within BT0252 at 280.282 kb on - strand, within BT0252 at 280.530 kb on - strand, within BT0252 at 280.756 kb on + strand, within BT0252 at 280.757 kb on - strand, within BT0252 at 280.757 kb on - strand, within BT0252 at 280.963 kb on - strand at 281.049 kb on + strand at 281.092 kb on - strand at 281.112 kb on + strand at 281.113 kb on - strand at 281.150 kb on + strand at 281.182 kb on + strand at 281.243 kb on + strand at 281.255 kb on + strand at 281.366 kb on + strand at 281.415 kb on - strand, within BT0253 at 281.424 kb on + strand, within BT0253 at 281.425 kb on - strand, within BT0253 at 281.431 kb on - strand, within BT0253 at 281.483 kb on - strand, within BT0253 at 281.543 kb on + strand, within BT0253 at 281.559 kb on + strand, within BT0253 at 281.560 kb on - strand, within BT0253 at 281.568 kb on + strand, within BT0253 at 281.569 kb on - strand, within BT0253 at 281.629 kb on + strand, within BT0253 at 281.764 kb on - strand, within BT0253 at 281.778 kb on - strand, within BT0253 at 281.832 kb on - strand, within BT0253 at 281.885 kb on + strand, within BT0253 at 281.975 kb on + strand, within BT0253 at 281.992 kb on + strand, within BT0253 at 282.014 kb on + strand, within BT0253 at 282.036 kb on - strand, within BT0253 at 282.068 kb on - strand at 282.072 kb on - strand at 282.147 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Ethanol 3.33 vol% remove 277,603 - -1.3 277,618 - -0.2 277,801 - -0.0 277,801 - -2.1 277,801 - +0.3 277,891 - +0.7 278,292 - BT0252 0.15 +0.1 278,302 + BT0252 0.15 -1.4 278,312 - BT0252 0.15 +1.2 278,332 + BT0252 0.16 +1.2 278,332 + BT0252 0.16 -0.1 278,333 - BT0252 0.16 +0.0 278,658 + BT0252 0.25 +1.5 278,956 - BT0252 0.34 +2.2 278,956 - BT0252 0.34 +0.9 279,119 - BT0252 0.39 -0.1 279,183 + BT0252 0.41 -0.1 279,184 - BT0252 0.41 +1.2 279,268 + BT0252 0.43 -0.6 279,343 - BT0252 0.46 +0.0 279,719 - BT0252 0.57 -1.2 279,908 + BT0252 0.62 +0.4 279,909 - BT0252 0.62 +1.0 279,925 - BT0252 0.63 +0.2 279,957 + BT0252 0.64 +0.1 279,999 - BT0252 0.65 -0.4 280,048 - BT0252 0.66 +0.2 280,258 + BT0252 0.73 -1.7 280,258 + BT0252 0.73 -1.7 280,258 + BT0252 0.73 +0.8 280,258 + BT0252 0.73 -0.8 280,282 - BT0252 0.73 -0.4 280,530 - BT0252 0.81 -1.7 280,756 + BT0252 0.87 -0.9 280,757 - BT0252 0.87 -0.1 280,757 - BT0252 0.87 -0.4 280,963 - -1.0 281,049 + -0.3 281,092 - +0.0 281,112 + -0.7 281,113 - +0.5 281,150 + +0.1 281,182 + -0.1 281,243 + -1.3 281,255 + -0.2 281,366 + -2.2 281,415 - BT0253 0.14 -2.0 281,424 + BT0253 0.15 +0.1 281,425 - BT0253 0.15 -2.0 281,431 - BT0253 0.16 +1.3 281,483 - BT0253 0.22 +1.0 281,543 + BT0253 0.29 +2.5 281,559 + BT0253 0.31 -0.5 281,560 - BT0253 0.31 -2.5 281,568 + BT0253 0.32 +0.4 281,569 - BT0253 0.32 +1.5 281,629 + BT0253 0.39 -0.3 281,764 - BT0253 0.55 +0.0 281,778 - BT0253 0.57 -0.3 281,832 - BT0253 0.63 -1.8 281,885 + BT0253 0.69 +0.2 281,975 + BT0253 0.80 +0.0 281,992 + BT0253 0.82 -0.4 282,014 + BT0253 0.85 -0.1 282,036 - BT0253 0.87 -0.2 282,068 - +0.4 282,072 - -0.3 282,147 - -0.7
Or see this region's nucleotide sequence