Experiment: Varel_Bryant_medium_Glucose with Lithocholic 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4560 and BT4561 are separated by 39 nucleotides BT4561 and BT4562 are separated by 6 nucleotides BT4562 and BT4563 are separated by 163 nucleotides BT4563 and BT4564 are separated by 20 nucleotides
BT4560: BT4560 - thiamine biosynthesis lipoprotein apbE precursor (NCBI ptt file), at 5,981,615 to 5,982,631
BT4560
BT4561: BT4561 - conserved hypothetical protein, putative integral membrane protein (NCBI ptt file), at 5,982,671 to 5,983,252
BT4561
BT4562: BT4562 - hypothetical protein (NCBI ptt file), at 5,983,259 to 5,983,825
BT4562
BT4563: BT4563 - hypothetical protein (NCBI ptt file), at 5,983,989 to 5,984,540
BT4563
BT4564: BT4564 - putative glycosyltransferase (NCBI ptt file), at 5,984,561 to 5,985,511
BT4564
Position (kb)
5983
5984 Strain fitness (log2 ratio)
-2
-1
0
1 at 5982.670 kb on - strand at 5982.684 kb on - strand at 5982.684 kb on - strand at 5982.693 kb on - strand at 5982.693 kb on - strand at 5982.790 kb on - strand, within BT4561 at 5982.819 kb on - strand, within BT4561 at 5982.911 kb on - strand, within BT4561 at 5982.911 kb on - strand, within BT4561 at 5983.004 kb on - strand, within BT4561 at 5983.004 kb on - strand, within BT4561 at 5983.050 kb on - strand, within BT4561 at 5983.163 kb on - strand, within BT4561 at 5983.351 kb on - strand, within BT4562 at 5983.389 kb on - strand, within BT4562 at 5983.582 kb on - strand, within BT4562 at 5983.707 kb on - strand, within BT4562 at 5984.199 kb on - strand, within BT4563 at 5984.204 kb on - strand, within BT4563 at 5984.211 kb on - strand, within BT4563 at 5984.426 kb on - strand, within BT4563 at 5984.426 kb on - strand, within BT4563 at 5984.515 kb on - strand at 5984.541 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Lithocholic 0.2 mg/ml remove 5,982,670 - -1.6 5,982,684 - +0.6 5,982,684 - -0.2 5,982,693 - -0.7 5,982,693 - +0.2 5,982,790 - BT4561 0.20 +0.4 5,982,819 - BT4561 0.25 -0.8 5,982,911 - BT4561 0.41 +0.6 5,982,911 - BT4561 0.41 +1.0 5,983,004 - BT4561 0.57 -1.1 5,983,004 - BT4561 0.57 -0.2 5,983,050 - BT4561 0.65 -0.6 5,983,163 - BT4561 0.85 -2.0 5,983,351 - BT4562 0.16 -1.4 5,983,389 - BT4562 0.23 -0.8 5,983,582 - BT4562 0.57 -1.9 5,983,707 - BT4562 0.79 -2.2 5,984,199 - BT4563 0.38 -0.8 5,984,204 - BT4563 0.39 -1.4 5,984,211 - BT4563 0.40 -1.2 5,984,426 - BT4563 0.79 -1.5 5,984,426 - BT4563 0.79 -1.3 5,984,515 - -1.8 5,984,541 - -1.3
Or see this region's nucleotide sequence