Experiment: R2A with Vogels_fungal_media 0.8X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS20020 and HSERO_RS20025 are separated by 131 nucleotides HSERO_RS20025 and HSERO_RS20030 are separated by 13 nucleotides
HSERO_RS20020: HSERO_RS20020 - enoyl-CoA hydratase, at 4,594,299 to 4,595,078
_RS20020
HSERO_RS20025: HSERO_RS20025 - feruloyl-CoA synthase, at 4,595,210 to 4,597,072
_RS20025
HSERO_RS20030: HSERO_RS20030 - acetyl-CoA acetyltransferase, at 4,597,086 to 4,598,339
_RS20030
Position (kb)
4595
4596
4597
4598 Strain fitness (log2 ratio)
-1
0
1
2 at 4594.327 kb on - strand at 4594.489 kb on + strand, within HSERO_RS20020 at 4594.498 kb on + strand, within HSERO_RS20020 at 4594.756 kb on - strand, within HSERO_RS20020 at 4594.782 kb on - strand, within HSERO_RS20020 at 4594.838 kb on - strand, within HSERO_RS20020 at 4595.057 kb on + strand at 4595.317 kb on + strand at 4595.413 kb on - strand at 4595.605 kb on + strand, within HSERO_RS20025 at 4595.616 kb on + strand, within HSERO_RS20025 at 4596.034 kb on + strand, within HSERO_RS20025 at 4596.045 kb on + strand, within HSERO_RS20025 at 4596.060 kb on - strand, within HSERO_RS20025 at 4596.234 kb on - strand, within HSERO_RS20025 at 4596.250 kb on - strand, within HSERO_RS20025 at 4596.308 kb on + strand, within HSERO_RS20025 at 4596.316 kb on - strand, within HSERO_RS20025 at 4596.563 kb on + strand, within HSERO_RS20025 at 4596.608 kb on + strand, within HSERO_RS20025 at 4596.837 kb on + strand, within HSERO_RS20025 at 4596.968 kb on - strand at 4597.000 kb on + strand at 4597.003 kb on - strand at 4597.054 kb on + strand at 4597.075 kb on - strand at 4597.119 kb on - strand at 4597.121 kb on + strand at 4597.178 kb on - strand at 4597.245 kb on - strand, within HSERO_RS20030 at 4597.437 kb on - strand, within HSERO_RS20030 at 4597.517 kb on + strand, within HSERO_RS20030 at 4597.866 kb on + strand at 4597.912 kb on + strand, within HSERO_RS20030
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.8X remove 4,594,327 - +2.2 4,594,489 + HSERO_RS20020 0.24 +0.4 4,594,498 + HSERO_RS20020 0.26 +0.0 4,594,756 - HSERO_RS20020 0.59 +0.8 4,594,782 - HSERO_RS20020 0.62 -0.1 4,594,838 - HSERO_RS20020 0.69 +0.4 4,595,057 + +0.0 4,595,317 + -0.1 4,595,413 - +0.8 4,595,605 + HSERO_RS20025 0.21 -1.5 4,595,616 + HSERO_RS20025 0.22 +0.4 4,596,034 + HSERO_RS20025 0.44 +0.2 4,596,045 + HSERO_RS20025 0.45 -0.3 4,596,060 - HSERO_RS20025 0.46 -0.5 4,596,234 - HSERO_RS20025 0.55 -0.0 4,596,250 - HSERO_RS20025 0.56 +0.1 4,596,308 + HSERO_RS20025 0.59 -0.2 4,596,316 - HSERO_RS20025 0.59 -0.0 4,596,563 + HSERO_RS20025 0.73 -0.3 4,596,608 + HSERO_RS20025 0.75 -0.0 4,596,837 + HSERO_RS20025 0.87 +0.9 4,596,968 - -0.2 4,597,000 + -1.6 4,597,003 - -0.0 4,597,054 + +0.8 4,597,075 - -0.5 4,597,119 - +0.3 4,597,121 + +1.9 4,597,178 - +0.2 4,597,245 - HSERO_RS20030 0.13 +0.9 4,597,437 - HSERO_RS20030 0.28 +0.5 4,597,517 + HSERO_RS20030 0.34 +0.6 4,597,866 + +2.2 4,597,912 + HSERO_RS20030 0.66 +0.9
Or see this region's nucleotide sequence