Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS03040

Experiment: Fungal_growth_media_mCAFEs_v1 with Iron (II) 0.209 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS03030 and BWI76_RS03035 overlap by 1 nucleotidesBWI76_RS03035 and BWI76_RS03040 are separated by 98 nucleotidesBWI76_RS03040 and BWI76_RS03045 are separated by 158 nucleotidesBWI76_RS03045 and BWI76_RS03050 overlap by 11 nucleotides BWI76_RS03030: BWI76_RS03030 - valine--tRNA ligase, at 619,030 to 621,885 _RS03030 BWI76_RS03035: BWI76_RS03035 - DNA polymerase III subunit chi, at 621,885 to 622,328 _RS03035 BWI76_RS03040: BWI76_RS03040 - leucyl aminopeptidase, at 622,427 to 623,938 _RS03040 BWI76_RS03045: BWI76_RS03045 - hypothetical protein, at 624,097 to 624,216 _RS03045 BWI76_RS03050: BWI76_RS03050 - LPS export ABC transporter permease LptF, at 624,206 to 625,303 _RS03050 Position (kb) 622 623 624Strain fitness (log2 ratio) -1 0 1at 622.466 kb on - strandat 622.850 kb on - strand, within BWI76_RS03040at 622.895 kb on - strand, within BWI76_RS03040at 623.609 kb on - strand, within BWI76_RS03040at 623.808 kb on - strandat 623.962 kb on - strandat 624.198 kb on + strand, within BWI76_RS03045

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Per-strain Table

Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Iron (II) 0.209 mg/mL
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622,466 - +0.6
622,850 - BWI76_RS03040 0.28 -1.5
622,895 - BWI76_RS03040 0.31 -1.3
623,609 - BWI76_RS03040 0.78 -0.2
623,808 - -0.4
623,962 - +0.4
624,198 + BWI76_RS03045 0.84 +1.0

Or see this region's nucleotide sequence