Experiment: KB with D-Salicin 240 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1565 and Psyr_1566 are separated by 66 nucleotides Psyr_1566 and Psyr_1567 are separated by 210 nucleotides Psyr_1567 and Psyr_1568 are separated by 4 nucleotides
Psyr_1565: Psyr_1565 - D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding protein, at 1,761,509 to 1,762,441
_1565
Psyr_1566: Psyr_1566 - Nitroreductase, at 1,762,508 to 1,763,101
_1566
Psyr_1567: Psyr_1567 - Uncharacterized conserved protein UCP006173, at 1,763,312 to 1,763,761
_1567
Psyr_1568: Psyr_1568 - conserved hypothetical protein, at 1,763,766 to 1,764,146
_1568
Position (kb)
1762
1763
1764 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1761.545 kb on + strand at 1761.545 kb on + strand at 1761.545 kb on + strand at 1761.545 kb on + strand at 1761.546 kb on - strand at 1761.546 kb on - strand at 1761.546 kb on - strand at 1761.546 kb on - strand at 1761.818 kb on + strand, within Psyr_1565 at 1761.818 kb on + strand, within Psyr_1565 at 1761.818 kb on + strand, within Psyr_1565 at 1761.818 kb on + strand, within Psyr_1565 at 1761.818 kb on + strand, within Psyr_1565 at 1761.818 kb on + strand, within Psyr_1565 at 1761.818 kb on + strand, within Psyr_1565 at 1761.818 kb on + strand, within Psyr_1565 at 1761.819 kb on - strand, within Psyr_1565 at 1761.819 kb on - strand, within Psyr_1565 at 1761.819 kb on - strand, within Psyr_1565 at 1761.819 kb on - strand, within Psyr_1565 at 1761.819 kb on - strand, within Psyr_1565 at 1761.989 kb on + strand, within Psyr_1565 at 1761.990 kb on - strand, within Psyr_1565 at 1761.990 kb on - strand, within Psyr_1565 at 1762.079 kb on + strand, within Psyr_1565 at 1762.079 kb on + strand, within Psyr_1565 at 1762.080 kb on - strand, within Psyr_1565 at 1762.080 kb on - strand, within Psyr_1565 at 1762.080 kb on - strand, within Psyr_1565 at 1762.080 kb on - strand, within Psyr_1565 at 1762.080 kb on - strand, within Psyr_1565 at 1762.080 kb on - strand, within Psyr_1565 at 1762.304 kb on + strand, within Psyr_1565 at 1762.304 kb on + strand, within Psyr_1565 at 1762.304 kb on + strand, within Psyr_1565 at 1762.510 kb on - strand at 1762.584 kb on + strand, within Psyr_1566 at 1762.584 kb on + strand, within Psyr_1566 at 1762.657 kb on + strand, within Psyr_1566 at 1762.658 kb on - strand, within Psyr_1566 at 1762.743 kb on + strand, within Psyr_1566 at 1762.897 kb on - strand, within Psyr_1566 at 1762.929 kb on + strand, within Psyr_1566 at 1762.929 kb on + strand, within Psyr_1566 at 1762.930 kb on - strand, within Psyr_1566 at 1762.930 kb on - strand, within Psyr_1566 at 1762.954 kb on + strand, within Psyr_1566 at 1762.956 kb on + strand, within Psyr_1566 at 1762.956 kb on + strand, within Psyr_1566 at 1762.956 kb on + strand, within Psyr_1566 at 1762.957 kb on - strand, within Psyr_1566 at 1762.957 kb on - strand, within Psyr_1566 at 1763.196 kb on - strand at 1763.264 kb on + strand at 1763.264 kb on + strand at 1763.265 kb on - strand at 1763.270 kb on + strand at 1763.271 kb on - strand at 1763.447 kb on + strand, within Psyr_1567 at 1763.450 kb on + strand, within Psyr_1567 at 1763.450 kb on + strand, within Psyr_1567 at 1763.450 kb on + strand, within Psyr_1567 at 1763.450 kb on + strand, within Psyr_1567 at 1763.677 kb on + strand, within Psyr_1567 at 1763.677 kb on + strand, within Psyr_1567 at 1763.677 kb on + strand, within Psyr_1567 at 1763.738 kb on + strand at 1763.739 kb on - strand at 1763.767 kb on + strand at 1763.767 kb on + strand at 1763.767 kb on + strand at 1763.768 kb on - strand at 1763.768 kb on - strand at 1763.768 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with D-Salicin 240 ug/ml remove 1,761,545 + -0.2 1,761,545 + +0.0 1,761,545 + -1.6 1,761,545 + +0.3 1,761,546 - +0.8 1,761,546 - +0.7 1,761,546 - -1.4 1,761,546 - +0.7 1,761,818 + Psyr_1565 0.33 +0.3 1,761,818 + Psyr_1565 0.33 +0.5 1,761,818 + Psyr_1565 0.33 -1.3 1,761,818 + Psyr_1565 0.33 -0.4 1,761,818 + Psyr_1565 0.33 +0.2 1,761,818 + Psyr_1565 0.33 -0.2 1,761,818 + Psyr_1565 0.33 -0.9 1,761,818 + Psyr_1565 0.33 +0.3 1,761,819 - Psyr_1565 0.33 -0.0 1,761,819 - Psyr_1565 0.33 +0.9 1,761,819 - Psyr_1565 0.33 -0.1 1,761,819 - Psyr_1565 0.33 +0.4 1,761,819 - Psyr_1565 0.33 +0.5 1,761,989 + Psyr_1565 0.51 -0.5 1,761,990 - Psyr_1565 0.52 +1.3 1,761,990 - Psyr_1565 0.52 +1.1 1,762,079 + Psyr_1565 0.61 -0.3 1,762,079 + Psyr_1565 0.61 +0.7 1,762,080 - Psyr_1565 0.61 -1.6 1,762,080 - Psyr_1565 0.61 -1.4 1,762,080 - Psyr_1565 0.61 +0.7 1,762,080 - Psyr_1565 0.61 -0.2 1,762,080 - Psyr_1565 0.61 +0.5 1,762,080 - Psyr_1565 0.61 +0.3 1,762,304 + Psyr_1565 0.85 +0.1 1,762,304 + Psyr_1565 0.85 -0.3 1,762,304 + Psyr_1565 0.85 +1.2 1,762,510 - +0.5 1,762,584 + Psyr_1566 0.13 +0.0 1,762,584 + Psyr_1566 0.13 +0.7 1,762,657 + Psyr_1566 0.25 -0.2 1,762,658 - Psyr_1566 0.25 +0.3 1,762,743 + Psyr_1566 0.40 -0.5 1,762,897 - Psyr_1566 0.65 -0.2 1,762,929 + Psyr_1566 0.71 +0.0 1,762,929 + Psyr_1566 0.71 -0.3 1,762,930 - Psyr_1566 0.71 -2.4 1,762,930 - Psyr_1566 0.71 -0.9 1,762,954 + Psyr_1566 0.75 -0.9 1,762,956 + Psyr_1566 0.75 -1.4 1,762,956 + Psyr_1566 0.75 -0.6 1,762,956 + Psyr_1566 0.75 -0.3 1,762,957 - Psyr_1566 0.76 +0.1 1,762,957 - Psyr_1566 0.76 -0.9 1,763,196 - -0.3 1,763,264 + -2.0 1,763,264 + -0.1 1,763,265 - -2.6 1,763,270 + +0.4 1,763,271 - -0.6 1,763,447 + Psyr_1567 0.30 +0.3 1,763,450 + Psyr_1567 0.31 +0.3 1,763,450 + Psyr_1567 0.31 +0.5 1,763,450 + Psyr_1567 0.31 +2.1 1,763,450 + Psyr_1567 0.31 -0.0 1,763,677 + Psyr_1567 0.81 -1.1 1,763,677 + Psyr_1567 0.81 +0.1 1,763,677 + Psyr_1567 0.81 +0.1 1,763,738 + +0.7 1,763,739 - +1.7 1,763,767 + -0.5 1,763,767 + -0.9 1,763,767 + +0.0 1,763,768 - -0.6 1,763,768 - +0.1 1,763,768 - -0.5
Or see this region's nucleotide sequence