Experiment: Varel_Bryant_medium_Glucose with Deoxycholic 0.075 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0535 and BT0536 are separated by 172 nucleotides BT0536 and BT0537 overlap by 4 nucleotides BT0537 and BT0538 overlap by 11 nucleotides BT0538 and BT0539 are separated by 170 nucleotides
BT0535: BT0535 - iron-containing alcohol dehydrogenase (NCBI ptt file), at 658,163 to 659,308
BT0535
BT0536: BT0536 - two-component system sensor protein (NCBI ptt file), at 659,481 to 660,041
BT0536
BT0537: BT0537 - conserved hypothetical protein (NCBI ptt file), at 660,038 to 661,102
BT0537
BT0538: BT0538 - hypothetical protein (NCBI ptt file), at 661,092 to 661,277
BT0538
BT0539: BT0539 - putative Na+/sulphate transporter (NCBI ptt file), at 661,448 to 663,307
BT0539
Position (kb) 660
661
662 Strain fitness (log2 ratio) -2
-1
0
1
2 at 659.066 kb on + strand, within BT0535 at 659.134 kb on + strand, within BT0535 at 659.246 kb on + strand at 659.247 kb on - strand at 659.289 kb on + strand at 659.289 kb on + strand at 659.352 kb on + strand at 659.394 kb on - strand at 659.394 kb on - strand at 659.396 kb on + strand at 659.397 kb on - strand at 659.404 kb on - strand at 659.407 kb on - strand at 659.453 kb on - strand at 659.459 kb on + strand at 659.460 kb on - strand at 659.544 kb on - strand, within BT0536 at 659.702 kb on - strand, within BT0536 at 659.702 kb on - strand, within BT0536 at 659.997 kb on + strand at 659.998 kb on - strand at 660.033 kb on + strand at 660.033 kb on + strand at 660.120 kb on - strand at 660.323 kb on - strand, within BT0537 at 660.325 kb on + strand, within BT0537 at 660.427 kb on + strand, within BT0537 at 660.428 kb on - strand, within BT0537 at 660.429 kb on + strand, within BT0537 at 660.479 kb on + strand, within BT0537 at 660.501 kb on + strand, within BT0537 at 660.524 kb on + strand, within BT0537 at 660.533 kb on + strand, within BT0537 at 660.671 kb on + strand, within BT0537 at 660.732 kb on - strand, within BT0537 at 660.796 kb on - strand, within BT0537 at 660.818 kb on + strand, within BT0537 at 660.871 kb on - strand, within BT0537 at 660.874 kb on - strand, within BT0537 at 660.902 kb on + strand, within BT0537 at 660.925 kb on + strand, within BT0537 at 661.000 kb on - strand at 661.069 kb on + strand at 661.090 kb on + strand at 661.091 kb on - strand at 661.414 kb on + strand at 661.448 kb on - strand at 661.502 kb on - strand at 661.528 kb on - strand at 661.560 kb on + strand at 661.564 kb on - strand at 661.643 kb on - strand, within BT0539 at 661.672 kb on - strand, within BT0539 at 661.687 kb on + strand, within BT0539 at 661.729 kb on + strand, within BT0539 at 661.729 kb on + strand, within BT0539 at 661.730 kb on - strand, within BT0539 at 661.730 kb on - strand, within BT0539 at 661.870 kb on + strand, within BT0539 at 662.027 kb on - strand, within BT0539 at 662.063 kb on - strand, within BT0539 at 662.063 kb on - strand, within BT0539 at 662.086 kb on - strand, within BT0539
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Deoxycholic 0.075 mg/ml remove 659,066 + BT0535 0.79 -0.1 659,134 + BT0535 0.85 +0.3 659,246 + -0.7 659,247 - +0.8 659,289 + +0.0 659,289 + -2.4 659,352 + +0.4 659,394 - +0.1 659,394 - -0.4 659,396 + -0.1 659,397 - -0.8 659,404 - +1.5 659,407 - +0.3 659,453 - +0.0 659,459 + -1.6 659,460 - +2.3 659,544 - BT0536 0.11 -1.8 659,702 - BT0536 0.39 +0.1 659,702 - BT0536 0.39 -0.4 659,997 + -0.2 659,998 - +0.0 660,033 + +0.2 660,033 + -1.0 660,120 - -0.4 660,323 - BT0537 0.27 -0.4 660,325 + BT0537 0.27 +0.1 660,427 + BT0537 0.37 -0.4 660,428 - BT0537 0.37 -2.6 660,429 + BT0537 0.37 +0.5 660,479 + BT0537 0.41 +0.3 660,501 + BT0537 0.43 -0.9 660,524 + BT0537 0.46 -0.4 660,533 + BT0537 0.46 -2.0 660,671 + BT0537 0.59 -0.4 660,732 - BT0537 0.65 -0.6 660,796 - BT0537 0.71 +0.1 660,818 + BT0537 0.73 -2.3 660,871 - BT0537 0.78 -0.1 660,874 - BT0537 0.78 -1.3 660,902 + BT0537 0.81 +1.8 660,925 + BT0537 0.83 -0.5 661,000 - -0.3 661,069 + -1.2 661,090 + -1.3 661,091 - +0.3 661,414 + +0.5 661,448 - +0.9 661,502 - -1.4 661,528 - -0.9 661,560 + +0.3 661,564 - +0.7 661,643 - BT0539 0.10 -0.9 661,672 - BT0539 0.12 +2.7 661,687 + BT0539 0.13 -0.9 661,729 + BT0539 0.15 -1.2 661,729 + BT0539 0.15 -0.1 661,730 - BT0539 0.15 +0.9 661,730 - BT0539 0.15 +1.0 661,870 + BT0539 0.23 -0.6 662,027 - BT0539 0.31 -1.9 662,063 - BT0539 0.33 +1.8 662,063 - BT0539 0.33 -0.1 662,086 - BT0539 0.34 +0.8
Or see this region's nucleotide sequence