Experiment: KB with Quercetin 360 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4365 and Psyr_4366 are separated by 28 nucleotides Psyr_4366 and Psyr_4367 are separated by 9 nucleotides Psyr_4367 and Psyr_4368 are separated by 60 nucleotides Psyr_4368 and Psyr_4369 are separated by 13 nucleotides
Psyr_4365: Psyr_4365 - cell elongation-specific peptidoglycan D,D-transpeptidase / peptidoglycan glycosyltransferase, at 5,218,555 to 5,220,450
_4365
Psyr_4366: Psyr_4366 - Protein of unknown function DUF163, at 5,220,479 to 5,220,946
_4366
Psyr_4367: Psyr_4367 - Iojap-related protein, at 5,220,956 to 5,221,342
_4367
Psyr_4368: Psyr_4368 - nicotinate-nucleotide adenylyltransferase, at 5,221,403 to 5,222,071
_4368
Psyr_4369: Psyr_4369 - glutamate-5-semialdehyde dehydrogenase, at 5,222,085 to 5,223,350
_4369
Position (kb)
5220
5221
5222 Strain fitness (log2 ratio)
-2
-1
0
1 at 5220.453 kb on - strand at 5220.469 kb on - strand at 5220.469 kb on - strand at 5220.481 kb on - strand at 5220.481 kb on - strand at 5220.481 kb on - strand at 5220.481 kb on - strand at 5220.481 kb on - strand at 5220.481 kb on - strand at 5220.481 kb on - strand at 5220.481 kb on - strand at 5220.481 kb on - strand at 5220.490 kb on - strand at 5220.523 kb on - strand at 5220.523 kb on - strand at 5220.958 kb on - strand at 5220.958 kb on - strand at 5221.222 kb on - strand, within Psyr_4367 at 5221.222 kb on - strand, within Psyr_4367 at 5221.222 kb on - strand, within Psyr_4367 at 5221.222 kb on - strand, within Psyr_4367 at 5221.222 kb on - strand, within Psyr_4367 at 5221.222 kb on - strand, within Psyr_4367 at 5221.222 kb on - strand, within Psyr_4367 at 5221.222 kb on - strand, within Psyr_4367 at 5221.222 kb on - strand, within Psyr_4367 at 5221.343 kb on - strand at 5221.343 kb on - strand at 5221.345 kb on - strand at 5221.345 kb on - strand at 5221.345 kb on - strand at 5221.345 kb on - strand at 5221.345 kb on - strand at 5221.345 kb on - strand at 5221.345 kb on - strand at 5221.345 kb on - strand at 5221.363 kb on - strand at 5221.363 kb on - strand at 5221.363 kb on - strand at 5221.363 kb on - strand at 5221.363 kb on - strand at 5221.363 kb on - strand at 5221.363 kb on - strand at 5222.126 kb on - strand at 5222.126 kb on - strand at 5222.126 kb on - strand at 5222.126 kb on - strand at 5222.126 kb on - strand at 5222.178 kb on - strand at 5222.178 kb on - strand at 5222.178 kb on - strand at 5222.184 kb on - strand at 5222.184 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Quercetin 360 ug/ml remove 5,220,453 - -1.0 5,220,469 - +1.0 5,220,469 - -0.2 5,220,481 - -1.5 5,220,481 - -0.2 5,220,481 - +0.3 5,220,481 - -1.8 5,220,481 - -0.5 5,220,481 - +0.8 5,220,481 - +0.4 5,220,481 - -1.2 5,220,481 - +1.0 5,220,490 - -1.7 5,220,523 - -0.1 5,220,523 - -1.4 5,220,958 - -0.5 5,220,958 - +0.6 5,221,222 - Psyr_4367 0.69 -0.2 5,221,222 - Psyr_4367 0.69 -1.0 5,221,222 - Psyr_4367 0.69 +0.2 5,221,222 - Psyr_4367 0.69 -0.1 5,221,222 - Psyr_4367 0.69 -2.6 5,221,222 - Psyr_4367 0.69 -0.4 5,221,222 - Psyr_4367 0.69 -1.6 5,221,222 - Psyr_4367 0.69 -0.6 5,221,222 - Psyr_4367 0.69 -0.3 5,221,343 - +0.4 5,221,343 - -1.3 5,221,345 - -0.1 5,221,345 - -0.9 5,221,345 - -0.0 5,221,345 - +1.0 5,221,345 - +1.0 5,221,345 - +0.4 5,221,345 - -0.8 5,221,345 - +0.4 5,221,363 - +0.1 5,221,363 - -1.2 5,221,363 - -0.7 5,221,363 - +0.5 5,221,363 - +1.1 5,221,363 - +0.2 5,221,363 - +0.6 5,222,126 - +1.1 5,222,126 - -0.5 5,222,126 - -0.1 5,222,126 - -0.2 5,222,126 - -1.2 5,222,178 - -1.4 5,222,178 - -1.7 5,222,178 - -0.3 5,222,184 - -0.4 5,222,184 - -0.3
Or see this region's nucleotide sequence