Experiment: KB with Quercetin 360 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2820 and Psyr_2821 overlap by 4 nucleotides Psyr_2821 and Psyr_2822 overlap by 4 nucleotides Psyr_2822 and Psyr_2823 are separated by 81 nucleotides
Psyr_2820: Psyr_2820 - RecT protein, at 3,394,228 to 3,395,115
_2820
Psyr_2821: Psyr_2821 - conserved hypothetical protein, at 3,395,112 to 3,396,773
_2821
Psyr_2822: Psyr_2822 - hypothetical protein, at 3,396,770 to 3,397,132
_2822
Psyr_2823: Psyr_2823 - hypothetical protein, at 3,397,214 to 3,397,597
_2823
Position (kb) 3395
3396
3397 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 3394.177 kb on - strand at 3394.227 kb on + strand at 3394.227 kb on + strand at 3394.228 kb on - strand at 3394.316 kb on + strand at 3394.534 kb on - strand, within Psyr_2820 at 3394.831 kb on + strand, within Psyr_2820 at 3394.832 kb on - strand, within Psyr_2820 at 3394.945 kb on + strand, within Psyr_2820 at 3395.047 kb on + strand at 3395.048 kb on - strand at 3395.048 kb on - strand at 3395.123 kb on + strand at 3395.169 kb on + strand at 3395.170 kb on - strand at 3395.289 kb on + strand, within Psyr_2821 at 3395.289 kb on + strand, within Psyr_2821 at 3395.290 kb on - strand, within Psyr_2821 at 3395.290 kb on - strand, within Psyr_2821 at 3395.290 kb on - strand, within Psyr_2821 at 3395.290 kb on - strand, within Psyr_2821 at 3395.290 kb on - strand, within Psyr_2821 at 3395.290 kb on - strand, within Psyr_2821 at 3395.502 kb on + strand, within Psyr_2821 at 3395.502 kb on + strand, within Psyr_2821 at 3395.503 kb on - strand, within Psyr_2821 at 3395.503 kb on - strand, within Psyr_2821 at 3395.503 kb on - strand, within Psyr_2821 at 3395.505 kb on + strand, within Psyr_2821 at 3395.505 kb on + strand, within Psyr_2821 at 3395.602 kb on - strand, within Psyr_2821 at 3395.674 kb on - strand, within Psyr_2821 at 3395.747 kb on - strand, within Psyr_2821 at 3396.599 kb on + strand, within Psyr_2821 at 3396.600 kb on - strand, within Psyr_2821 at 3396.600 kb on - strand, within Psyr_2821 at 3396.600 kb on - strand, within Psyr_2821 at 3396.600 kb on - strand, within Psyr_2821 at 3396.600 kb on - strand, within Psyr_2821 at 3396.868 kb on - strand, within Psyr_2822 at 3396.938 kb on + strand, within Psyr_2822 at 3396.938 kb on + strand, within Psyr_2822 at 3396.962 kb on + strand, within Psyr_2822 at 3396.962 kb on + strand, within Psyr_2822 at 3396.962 kb on + strand, within Psyr_2822 at 3396.963 kb on - strand, within Psyr_2822 at 3396.963 kb on - strand, within Psyr_2822 at 3397.094 kb on + strand, within Psyr_2822 at 3397.094 kb on + strand, within Psyr_2822 at 3397.106 kb on + strand at 3397.211 kb on + strand at 3397.558 kb on + strand, within Psyr_2823 at 3397.559 kb on - strand at 3397.616 kb on + strand at 3397.617 kb on - strand at 3397.617 kb on - strand at 3397.617 kb on - strand at 3397.617 kb on - strand at 3397.617 kb on - strand at 3397.621 kb on - strand at 3397.664 kb on + strand at 3397.664 kb on + strand at 3397.664 kb on + strand at 3397.664 kb on + strand at 3397.665 kb on - strand at 3397.721 kb on + strand at 3397.757 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Quercetin 360 ug/ml remove 3,394,177 - +0.4 3,394,227 + -0.3 3,394,227 + -0.7 3,394,228 - +0.8 3,394,316 + -3.3 3,394,534 - Psyr_2820 0.34 +0.7 3,394,831 + Psyr_2820 0.68 -0.2 3,394,832 - Psyr_2820 0.68 +1.1 3,394,945 + Psyr_2820 0.81 -0.4 3,395,047 + -0.3 3,395,048 - +0.6 3,395,048 - -0.1 3,395,123 + -0.2 3,395,169 + +2.2 3,395,170 - -0.3 3,395,289 + Psyr_2821 0.11 +0.9 3,395,289 + Psyr_2821 0.11 -0.0 3,395,290 - Psyr_2821 0.11 +1.4 3,395,290 - Psyr_2821 0.11 +0.1 3,395,290 - Psyr_2821 0.11 +1.5 3,395,290 - Psyr_2821 0.11 -0.3 3,395,290 - Psyr_2821 0.11 -1.0 3,395,290 - Psyr_2821 0.11 -0.2 3,395,502 + Psyr_2821 0.23 -0.5 3,395,502 + Psyr_2821 0.23 +0.4 3,395,503 - Psyr_2821 0.24 +0.9 3,395,503 - Psyr_2821 0.24 -0.5 3,395,503 - Psyr_2821 0.24 +0.3 3,395,505 + Psyr_2821 0.24 +1.8 3,395,505 + Psyr_2821 0.24 +0.4 3,395,602 - Psyr_2821 0.29 +1.4 3,395,674 - Psyr_2821 0.34 +0.6 3,395,747 - Psyr_2821 0.38 -0.9 3,396,599 + Psyr_2821 0.89 -0.8 3,396,600 - Psyr_2821 0.90 -0.1 3,396,600 - Psyr_2821 0.90 +1.3 3,396,600 - Psyr_2821 0.90 +1.6 3,396,600 - Psyr_2821 0.90 -1.4 3,396,600 - Psyr_2821 0.90 -0.7 3,396,868 - Psyr_2822 0.27 -1.4 3,396,938 + Psyr_2822 0.46 +2.7 3,396,938 + Psyr_2822 0.46 +1.0 3,396,962 + Psyr_2822 0.53 +0.3 3,396,962 + Psyr_2822 0.53 -0.3 3,396,962 + Psyr_2822 0.53 -4.0 3,396,963 - Psyr_2822 0.53 +0.3 3,396,963 - Psyr_2822 0.53 +2.3 3,397,094 + Psyr_2822 0.89 +0.5 3,397,094 + Psyr_2822 0.89 +0.9 3,397,106 + -1.0 3,397,211 + -0.3 3,397,558 + Psyr_2823 0.90 -1.3 3,397,559 - +0.6 3,397,616 + -2.2 3,397,617 - -0.7 3,397,617 - +0.4 3,397,617 - -0.3 3,397,617 - +0.5 3,397,617 - +1.3 3,397,621 - -0.8 3,397,664 + -2.7 3,397,664 + +0.9 3,397,664 + -1.8 3,397,664 + -0.0 3,397,665 - +0.6 3,397,721 + -1.7 3,397,757 - +0.4
Or see this region's nucleotide sequence