Experiment: KB with Quercetin 360 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2277 and Psyr_2278 are separated by 546 nucleotides Psyr_2278 and Psyr_2279 are separated by 206 nucleotides Psyr_2279 and Psyr_2280 are separated by 323 nucleotides
Psyr_2277: Psyr_2277 - ammonium transporter, at 2,643,672 to 2,644,997
_2277
Psyr_2278: Psyr_2278 - luciferase, at 2,645,544 to 2,646,581
_2278
Psyr_2279: Psyr_2279 - conserved hypothetical protein, at 2,646,788 to 2,647,123
_2279
Psyr_2280: Psyr_2280 - Alkanesulfonate monooxygenase, at 2,647,447 to 2,648,547
_2280
Position (kb)
2645
2646
2647 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2644.704 kb on + strand, within Psyr_2277 at 2644.763 kb on - strand, within Psyr_2277 at 2644.986 kb on + strand at 2644.987 kb on - strand at 2645.041 kb on + strand at 2645.041 kb on + strand at 2645.042 kb on - strand at 2645.042 kb on - strand at 2645.042 kb on - strand at 2645.043 kb on + strand at 2645.043 kb on + strand at 2645.043 kb on + strand at 2645.043 kb on + strand at 2645.043 kb on + strand at 2645.043 kb on + strand at 2645.043 kb on + strand at 2645.043 kb on + strand at 2645.043 kb on + strand at 2645.043 kb on + strand at 2645.044 kb on - strand at 2645.044 kb on - strand at 2645.044 kb on - strand at 2645.044 kb on - strand at 2645.044 kb on - strand at 2645.044 kb on - strand at 2645.044 kb on - strand at 2645.044 kb on - strand at 2645.272 kb on + strand at 2645.318 kb on - strand at 2645.367 kb on + strand at 2645.494 kb on + strand at 2645.494 kb on + strand at 2645.660 kb on - strand, within Psyr_2278 at 2645.702 kb on + strand, within Psyr_2278 at 2645.703 kb on - strand, within Psyr_2278 at 2645.703 kb on - strand, within Psyr_2278 at 2645.703 kb on - strand, within Psyr_2278 at 2645.828 kb on - strand, within Psyr_2278 at 2646.146 kb on - strand, within Psyr_2278 at 2646.169 kb on - strand, within Psyr_2278 at 2646.169 kb on - strand, within Psyr_2278 at 2646.397 kb on + strand, within Psyr_2278 at 2646.434 kb on - strand, within Psyr_2278 at 2646.434 kb on - strand, within Psyr_2278 at 2646.434 kb on - strand, within Psyr_2278 at 2646.588 kb on + strand at 2646.588 kb on + strand at 2646.588 kb on + strand at 2646.588 kb on + strand at 2646.589 kb on - strand at 2646.597 kb on + strand at 2646.646 kb on - strand at 2646.812 kb on - strand at 2646.812 kb on - strand at 2646.813 kb on + strand at 2646.813 kb on + strand at 2646.814 kb on - strand at 2646.814 kb on - strand at 2646.814 kb on - strand at 2646.814 kb on - strand at 2646.862 kb on + strand, within Psyr_2279 at 2646.928 kb on + strand, within Psyr_2279 at 2646.928 kb on + strand, within Psyr_2279 at 2646.928 kb on + strand, within Psyr_2279 at 2646.928 kb on + strand, within Psyr_2279 at 2646.928 kb on + strand, within Psyr_2279 at 2646.929 kb on - strand, within Psyr_2279 at 2646.930 kb on + strand, within Psyr_2279 at 2646.930 kb on + strand, within Psyr_2279 at 2646.930 kb on + strand, within Psyr_2279 at 2646.931 kb on - strand, within Psyr_2279 at 2646.931 kb on - strand, within Psyr_2279 at 2646.931 kb on - strand, within Psyr_2279 at 2646.945 kb on + strand, within Psyr_2279 at 2646.945 kb on + strand, within Psyr_2279 at 2646.945 kb on + strand, within Psyr_2279 at 2646.946 kb on - strand, within Psyr_2279 at 2646.946 kb on - strand, within Psyr_2279 at 2646.946 kb on - strand, within Psyr_2279 at 2646.946 kb on - strand, within Psyr_2279 at 2647.052 kb on + strand, within Psyr_2279 at 2647.194 kb on - strand at 2647.408 kb on + strand at 2647.447 kb on - strand at 2647.546 kb on + strand at 2647.547 kb on - strand at 2647.547 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Quercetin 360 ug/ml remove 2,644,704 + Psyr_2277 0.78 -0.8 2,644,763 - Psyr_2277 0.82 +0.2 2,644,986 + -0.8 2,644,987 - -0.2 2,645,041 + -0.1 2,645,041 + +0.1 2,645,042 - -0.3 2,645,042 - +0.1 2,645,042 - -0.9 2,645,043 + +0.1 2,645,043 + +0.7 2,645,043 + +1.2 2,645,043 + -0.1 2,645,043 + -1.9 2,645,043 + -0.3 2,645,043 + +0.5 2,645,043 + -0.2 2,645,043 + +1.7 2,645,043 + -0.9 2,645,044 - +0.1 2,645,044 - -1.0 2,645,044 - +0.8 2,645,044 - +0.7 2,645,044 - +0.7 2,645,044 - -0.3 2,645,044 - -0.9 2,645,044 - -2.1 2,645,272 + +0.3 2,645,318 - -0.2 2,645,367 + +0.7 2,645,494 + -0.1 2,645,494 + +1.0 2,645,660 - Psyr_2278 0.11 -0.1 2,645,702 + Psyr_2278 0.15 -2.7 2,645,703 - Psyr_2278 0.15 +0.0 2,645,703 - Psyr_2278 0.15 -0.9 2,645,703 - Psyr_2278 0.15 +1.7 2,645,828 - Psyr_2278 0.27 -1.3 2,646,146 - Psyr_2278 0.58 -1.6 2,646,169 - Psyr_2278 0.60 -1.1 2,646,169 - Psyr_2278 0.60 +2.2 2,646,397 + Psyr_2278 0.82 -1.1 2,646,434 - Psyr_2278 0.86 +0.7 2,646,434 - Psyr_2278 0.86 +0.1 2,646,434 - Psyr_2278 0.86 -2.7 2,646,588 + -0.6 2,646,588 + -0.3 2,646,588 + -0.7 2,646,588 + +0.2 2,646,589 - -0.9 2,646,597 + -0.3 2,646,646 - -1.1 2,646,812 - -1.7 2,646,812 - +1.3 2,646,813 + -1.4 2,646,813 + -0.9 2,646,814 - +2.7 2,646,814 - +0.7 2,646,814 - -0.2 2,646,814 - +0.5 2,646,862 + Psyr_2279 0.22 -0.9 2,646,928 + Psyr_2279 0.42 +0.7 2,646,928 + Psyr_2279 0.42 +0.7 2,646,928 + Psyr_2279 0.42 -0.7 2,646,928 + Psyr_2279 0.42 -0.5 2,646,928 + Psyr_2279 0.42 -1.8 2,646,929 - Psyr_2279 0.42 +1.2 2,646,930 + Psyr_2279 0.42 +0.2 2,646,930 + Psyr_2279 0.42 +0.4 2,646,930 + Psyr_2279 0.42 +0.3 2,646,931 - Psyr_2279 0.43 +1.2 2,646,931 - Psyr_2279 0.43 +0.2 2,646,931 - Psyr_2279 0.43 +0.7 2,646,945 + Psyr_2279 0.47 -0.7 2,646,945 + Psyr_2279 0.47 -0.8 2,646,945 + Psyr_2279 0.47 +0.2 2,646,946 - Psyr_2279 0.47 -0.7 2,646,946 - Psyr_2279 0.47 -3.6 2,646,946 - Psyr_2279 0.47 +1.4 2,646,946 - Psyr_2279 0.47 -1.3 2,647,052 + Psyr_2279 0.79 +0.1 2,647,194 - +0.1 2,647,408 + +0.1 2,647,447 - +0.7 2,647,546 + +0.0 2,647,547 - -1.7 2,647,547 - +0.5
Or see this region's nucleotide sequence