Experiment: KB with Quercetin 360 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2121 and Psyr_2122 are separated by 141 nucleotides Psyr_2122 and Psyr_2123 are separated by 11 nucleotides Psyr_2123 and Psyr_2124 are separated by 87 nucleotides
Psyr_2121: Psyr_2121 - regulatory protein, LysR:LysR, substrate-binding protein, at 2,478,901 to 2,479,821
_2121
Psyr_2122: Psyr_2122 - protocatechuate 3,4-dioxygenase, beta subunit, at 2,479,963 to 2,480,682
_2122
Psyr_2123: Psyr_2123 - protocatechuate 3,4-dioxygenase, alpha subunit, at 2,480,694 to 2,481,296
_2123
Psyr_2124: Psyr_2124 - Benzoate transport, at 2,481,384 to 2,482,733
_2124
Position (kb)
2479
2480
2481 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2479.205 kb on + strand, within Psyr_2121 at 2479.205 kb on + strand, within Psyr_2121 at 2479.306 kb on + strand, within Psyr_2121 at 2479.307 kb on - strand, within Psyr_2121 at 2479.328 kb on + strand, within Psyr_2121 at 2479.402 kb on + strand, within Psyr_2121 at 2479.402 kb on + strand, within Psyr_2121 at 2479.403 kb on - strand, within Psyr_2121 at 2479.403 kb on - strand, within Psyr_2121 at 2479.938 kb on - strand at 2479.938 kb on - strand at 2480.035 kb on + strand, within Psyr_2122 at 2480.035 kb on + strand, within Psyr_2122 at 2480.035 kb on + strand, within Psyr_2122 at 2480.197 kb on - strand, within Psyr_2122 at 2480.197 kb on - strand, within Psyr_2122 at 2480.228 kb on - strand, within Psyr_2122 at 2480.308 kb on + strand, within Psyr_2122 at 2480.309 kb on - strand, within Psyr_2122 at 2480.375 kb on - strand, within Psyr_2122 at 2480.375 kb on - strand, within Psyr_2122 at 2480.405 kb on - strand, within Psyr_2122 at 2480.500 kb on + strand, within Psyr_2122 at 2480.500 kb on + strand, within Psyr_2122 at 2480.895 kb on + strand, within Psyr_2123 at 2481.101 kb on + strand, within Psyr_2123 at 2481.102 kb on - strand, within Psyr_2123 at 2481.102 kb on - strand, within Psyr_2123 at 2481.102 kb on - strand, within Psyr_2123 at 2481.123 kb on + strand, within Psyr_2123 at 2481.123 kb on + strand, within Psyr_2123 at 2481.123 kb on + strand, within Psyr_2123 at 2481.123 kb on + strand, within Psyr_2123 at 2481.124 kb on - strand, within Psyr_2123 at 2481.222 kb on + strand, within Psyr_2123 at 2481.222 kb on + strand, within Psyr_2123 at 2481.223 kb on - strand, within Psyr_2123 at 2481.223 kb on - strand, within Psyr_2123 at 2481.363 kb on + strand at 2481.363 kb on + strand at 2481.369 kb on + strand at 2481.416 kb on + strand at 2481.416 kb on + strand at 2481.417 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Quercetin 360 ug/ml remove 2,479,205 + Psyr_2121 0.33 +1.2 2,479,205 + Psyr_2121 0.33 -1.6 2,479,306 + Psyr_2121 0.44 -1.1 2,479,307 - Psyr_2121 0.44 -0.6 2,479,328 + Psyr_2121 0.46 -0.3 2,479,402 + Psyr_2121 0.54 +1.1 2,479,402 + Psyr_2121 0.54 +2.0 2,479,403 - Psyr_2121 0.55 +0.4 2,479,403 - Psyr_2121 0.55 -1.0 2,479,938 - +1.7 2,479,938 - -1.0 2,480,035 + Psyr_2122 0.10 -1.5 2,480,035 + Psyr_2122 0.10 -2.1 2,480,035 + Psyr_2122 0.10 +0.0 2,480,197 - Psyr_2122 0.33 -2.3 2,480,197 - Psyr_2122 0.33 -3.4 2,480,228 - Psyr_2122 0.37 -0.6 2,480,308 + Psyr_2122 0.48 -0.7 2,480,309 - Psyr_2122 0.48 -1.8 2,480,375 - Psyr_2122 0.57 -1.6 2,480,375 - Psyr_2122 0.57 +0.4 2,480,405 - Psyr_2122 0.61 +1.1 2,480,500 + Psyr_2122 0.75 +0.1 2,480,500 + Psyr_2122 0.75 +0.7 2,480,895 + Psyr_2123 0.33 -0.3 2,481,101 + Psyr_2123 0.67 -0.9 2,481,102 - Psyr_2123 0.68 +3.0 2,481,102 - Psyr_2123 0.68 -0.2 2,481,102 - Psyr_2123 0.68 +0.2 2,481,123 + Psyr_2123 0.71 +1.7 2,481,123 + Psyr_2123 0.71 +0.7 2,481,123 + Psyr_2123 0.71 -1.4 2,481,123 + Psyr_2123 0.71 -0.1 2,481,124 - Psyr_2123 0.71 +2.0 2,481,222 + Psyr_2123 0.88 -0.1 2,481,222 + Psyr_2123 0.88 +0.2 2,481,223 - Psyr_2123 0.88 +0.4 2,481,223 - Psyr_2123 0.88 -0.5 2,481,363 + -0.2 2,481,363 + +2.6 2,481,369 + +0.5 2,481,416 + +1.0 2,481,416 + -3.5 2,481,417 - +0.5
Or see this region's nucleotide sequence